(Circular) (Six-base) MAPSORT of: pFN21AA0879.seq Check: 8629 from: 1 to: 6064 pFN21AA0879 6064 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 2147 2363 2616 3311 4127 5511 5779 Size: 463 52 216 253 695 816 1384 268 Cuts at: 5779 1632 Size: 1917 Fragments arranged by size: 1917 1384 816 695 463 268 253 216 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 3171 4793 3171 Size: 1622 4442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 2810 3807 5379 2810 Size: 997 1572 3495 Fragments arranged by size: 3495 1572 997 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 2138 2155 2934 3373 3599 3751 5860 1493 Size: 645 17 779 439 226 152 2109 1697 Fragments arranged by size: 2109 1697 779 645 439 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 3210 4618 160 Size: 872 2178 1408 1606 Fragments arranged by size: 2178 1606 1408 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2409 2442 2409 Size: 33 6031 BamHI G'GATC_C Cuts at: 2329 3394 3761 2329 Size: 1065 367 4632 Fragments arranged by size: 4632 1065 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3385 4840 618 Size: 325 217 627 1598 1455 1842 Fragments arranged by size: 1842 1598 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2804 3383 1391 Size: 1413 579 4072 Fragments arranged by size: 4072 1413 579 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2901 928 Size: 971 1002 4091 Fragments arranged by size: 4091 1002 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2107 3956 5166 2107 Size: 1849 1210 3005 Fragments arranged by size: 3005 1849 1210 BclI T'GATC_A Cuts at: 1548 2700 2713 1548 Size: 1152 13 4899 Fragments arranged by size: 4899 1152 13 BfrBI ATG'CAT Cuts at: 3040 3040 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4675 136 Size: 107 122 71 631 605 3003 1525 Fragments arranged by size: 3003 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2722 2798 3795 Size: 416 53 83 399 24 1305 76 997 Cuts at: 3795 4753 442 Size: 958 1753 Fragments arranged by size: 1753 1305 997 958 416 399 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 4724 1239 Size: 896 2589 2579 Fragments arranged by size: 2589 2579 896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 4189 5054 5352 2651 Size: 1538 865 298 3363 Fragments arranged by size: 3363 1538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 3114 3236 3348 6058 3114 Size: 122 112 2710 3120 Fragments arranged by size: 3120 2710 122 112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5839 Fragments arranged by size: 5839 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 1186 Size: 1275 4789 BseYI C'CCAG_C Cuts at: 5267 5763 5267 Size: 496 5568 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4125 4210 5281 4125 Size: 85 1071 4908 Fragments arranged by size: 4908 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2804 3383 4125 4210 5281 1391 Size: 399 1014 579 742 85 1071 2174 Fragments arranged by size: 2174 1071 1014 742 579 399 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3073 3419 844 Size: 2229 346 3489 Fragments arranged by size: 3489 2229 346 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 2525 4868 2525 Size: 2343 3721 BstEII G'GTnAC_C Cuts at: 1184 2365 3005 1184 Size: 1181 640 4243 Fragments arranged by size: 4243 1181 640 BstXI CCAn_nnnn'nTGG Cuts at: 2950 2950 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2329 3394 3761 4146 4163 5615 5701 5713 Size: 357 1065 367 385 17 1452 86 12 Cuts at: 5713 6059 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1065 385 367 357 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2260 3010 3058 513 Size: 552 1195 750 48 3519 Fragments arranged by size: 3519 1195 750 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3211 487 Size: 683 56 1985 3340 Fragments arranged by size: 3340 1985 683 56 BtsI GCAGTG_nn' Cuts at: 3580 4374 4394 3580 Size: 794 20 5250 Fragments arranged by size: 5250 794 20 ClaI AT'CG_AT Cuts at: 1554 2769 1554 Size: 1215 4849 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2578 3511 4400 Size: 54 1425 50 275 18 748 933 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 933 889 748 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 2452 3994 1353 Size: 715 384 1542 3423 Fragments arranged by size: 3423 1542 715 384 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2135 2147 2363 2616 3311 4127 Size: 393 463 40 12 216 253 695 816 Cuts at: 4127 4724 5511 5779 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 816 787 695 597 463 393 268 253 216 40 12 EcoO109I rG'GnC_Cy Cuts at: 3165 3165 Size: 6064 EcoRI G'AATT_C Cuts at: 2934 3373 3751 2934 Size: 439 378 5247 Fragments arranged by size: 5247 439 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2079 2323 2079 Size: 244 5820 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 5211 1678 Size: 3533 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2429 2461 3157 Size: 32 135 32 479 32 346 32 696 Cuts at: 3157 3189 4705 4737 4779 4811 1373 Size: 32 1516 32 42 32 2626 Fragments arranged by size: 2626 1516 696 479 346 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3196 3258 3408 3654 669 Size: 988 281 1258 62 150 246 3079 Fragments arranged by size: 3079 1258 988 281 246 150 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3258 3654 3258 Size: 396 5668 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 2973 3663 2973 Size: 690 5374 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 10 Size: 54 1425 343 748 3494 Fragments arranged by size: 3494 1425 748 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2877 3015 4022 4381 4540 518 Size: 648 1711 138 1007 359 159 2042 Fragments arranged by size: 2042 1711 1007 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 3480 4157 5305 5550 1606 Size: 1874 677 1148 245 2120 Fragments arranged by size: 2120 1874 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2260 3010 513 Size: 552 1195 750 3567 Fragments arranged by size: 3567 1195 750 552 NdeI CA'TA_TG Cuts at: 387 2874 387 Size: 2487 3577 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NruI TCG'CGA Cuts at: 3230 3230 Size: 6064 NsiI A_TGCA'T Cuts at: 3042 3042 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2189 3273 3422 4967 1132 Size: 97 960 1084 149 1545 2229 Fragments arranged by size: 2229 1545 1084 960 149 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 3136 1108 Size: 2028 4036 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3165 3165 Size: 6064 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 2042 2847 3634 2042 Size: 805 787 4472 Fragments arranged by size: 4472 805 787 PstI C_TGCA'G Cuts at: 830 3416 830 Size: 2586 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2227 2863 3412 4547 5228 5419 826 Size: 219 1182 636 549 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 636 549 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 4168 5069 5331 820 Size: 197 926 723 1502 901 262 1553 Fragments arranged by size: 1553 1502 926 901 723 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 2092 152 Size: 1940 4124 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 2503 3988 5 Size: 47 2451 1485 2081 Fragments arranged by size: 2451 2081 1485 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6064 StyI C'CwwG_G Cuts at: 513 1065 2260 3010 3503 513 Size: 552 1195 750 493 3074 Fragments arranged by size: 3074 1195 750 552 493 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2353 4091 4108 4261 4446 4891 325 Size: 1255 773 1738 17 153 185 445 1498 Fragments arranged by size: 1738 1498 1255 773 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 2654 1316 Size: 1338 4726 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 2898 4193 2898 Size: 1295 4769 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI