(Circular) (Six-base) MAPSORT of: pFN21AA0877.seq Check: 1094 from: 1 to: 6361 pFN21AA0877 6361 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 3399 3704 1937 Size: 864 598 305 4594 Fragments arranged by size: 4594 864 598 305 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 4424 5808 6076 1632 Size: 653 2139 1384 268 1917 Fragments arranged by size: 2139 1917 1384 653 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 2953 3069 5260 6237 1203 Size: 1750 116 2191 977 1327 Fragments arranged by size: 2191 1750 1327 977 116 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 3070 3072 1166 Size: 1904 2 4455 Fragments arranged by size: 4455 1904 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6361 AlwNI CAG_nnn'CTG Cuts at: 4104 5676 4104 Size: 1572 4789 ApaLI G'TGCA_C Cuts at: 4418 5574 4418 Size: 1156 5205 ApoI r'AATT_y Cuts at: 1493 2715 2765 3528 3670 3896 4048 6157 1493 Size: 1222 50 763 142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1222 763 226 152 142 50 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 3686 1870 Size: 73 1743 4545 Fragments arranged by size: 4545 1743 73 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3296 3682 5137 618 Size: 325 217 627 1509 386 1455 1842 Fragments arranged by size: 1842 1509 1455 627 386 325 217 BanII G_rGCy'C Cuts at: 1391 3680 1391 Size: 2289 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3372 928 Size: 971 1473 3917 Fragments arranged by size: 3917 1473 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4552 4586 1903 Size: 34 2615 34 3678 Fragments arranged by size: 3678 2615 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 2974 1548 Size: 1426 4935 BfrBI ATG'CAT Cuts at: 2999 2999 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4972 136 Size: 107 122 71 631 605 3300 1525 Fragments arranged by size: 3300 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6356 6356 Size: 6361 BlpI GC'TnA_GC Cuts at: 3778 3778 Size: 6361 Bme1580I G_kGCm'C Cuts at: 1790 4422 5578 1790 Size: 2632 1156 2573 Fragments arranged by size: 2632 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3196 4092 5050 Size: 416 53 83 399 24 1779 896 958 Cuts at: 5050 442 Size: 1753 Fragments arranged by size: 1779 1753 958 896 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2078 2078 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5021 1239 Size: 3782 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3082 3397 4486 5351 5649 3082 Size: 315 1089 865 298 3794 Fragments arranged by size: 3794 1089 865 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5024 882 Size: 617 3525 2219 Fragments arranged by size: 3525 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4152 493 Size: 711 2948 2702 Fragments arranged by size: 2948 2702 711 BsaBI GATnn'nnATC Cuts at: 6355 6355 Size: 6361 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4168 4550 5185 275 Size: 53 83 186 936 2635 382 635 1451 Fragments arranged by size: 2635 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4794 5192 5466 5613 6024 4794 Size: 398 274 147 411 5131 Fragments arranged by size: 5131 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2187 2217 2250 2280 2973 Size: 30 165 30 784 30 33 30 693 Cuts at: 2973 3003 1178 Size: 30 4536 Fragments arranged by size: 4536 784 693 165 33 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2067 1186 Size: 881 5480 BseYI C'CCAG_C Cuts at: 3557 5564 6060 3557 Size: 2007 496 3858 Fragments arranged by size: 3858 2007 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2829 3315 1163 Size: 573 1093 486 4209 Fragments arranged by size: 4209 1093 573 486 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4422 4507 5578 4422 Size: 85 1071 5205 Fragments arranged by size: 5205 1071 85 BsmI GAATG_Cn' Cuts at: 3872 3965 3872 Size: 93 6268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3680 4422 4507 5578 1391 Size: 399 1890 742 85 1071 2174 Fragments arranged by size: 2174 1890 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3716 844 Size: 2872 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2613 2999 3174 4856 5030 65 Size: 1183 1365 386 175 1682 174 1396 Fragments arranged by size: 1682 1396 1365 1183 386 175 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6361 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 3290 794 Size: 2496 3865 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5501 BstYI r'GATC_y Cuts at: 1972 2095 3500 3544 3691 4058 4443 4460 5912 Size: 123 1405 44 147 367 385 17 1452 Cuts at: 5912 5998 6010 6356 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1405 385 367 346 147 123 86 44 17 12 BstZ17I GTA'TAC Cuts at: 2802 3400 2802 Size: 598 5763 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4870 Fragments arranged by size: 4870 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5622 Fragments arranged by size: 5622 683 56 BtsI GCAGTG_nn' Cuts at: 3291 3877 4671 4691 3291 Size: 586 794 20 4961 Fragments arranged by size: 4961 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3185 3220 529 Size: 35 2621 35 3670 Fragments arranged by size: 3670 2621 35 35 DraI TTT'AAA Cuts at: 2133 3665 2133 Size: 1532 4829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5368 809 Size: 507 4052 1802 Fragments arranged by size: 4052 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326 Size: 54 1425 50 275 18 1978 889 1629 Cuts at: 6326 8 Size: 43 Fragments arranged by size: 1978 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3059 3474 4291 1353 Size: 1706 415 817 3423 Fragments arranged by size: 3423 1706 817 415 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4948 5322 5468 387 Size: 1064 138 3359 374 146 1280 Fragments arranged by size: 3359 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 4424 5021 5808 6076 1239 Size: 393 653 2139 597 787 268 1524 Fragments arranged by size: 2139 1524 787 653 597 393 268 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3263 3263 Size: 6361 FspI TGC'GCA Cuts at: 4867 4867 Size: 6361 HaeII r_GCGC'y Cuts at: 1678 5508 1678 Size: 3830 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2280 3036 3068 5002 Size: 32 135 32 676 32 756 32 1934 Cuts at: 5002 5034 5076 5108 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1934 756 676 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3705 3951 669 Size: 988 281 1767 246 3079 Fragments arranged by size: 3079 1767 988 281 246 HindIII A'AGCT_T Cuts at: 748 2275 2353 748 Size: 1527 78 4756 Fragments arranged by size: 4756 1527 78 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 3005 3960 3005 Size: 955 5406 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2018 3395 5475 5659 1305 Size: 713 1377 2080 184 2007 Fragments arranged by size: 2080 2007 1377 713 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4539 Fragments arranged by size: 4539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3070 3072 3138 4319 4678 4837 518 Size: 648 1904 2 66 1181 359 159 2042 Fragments arranged by size: 2042 1904 1181 648 359 159 66 2 MspA1I CmG'CkG Cuts at: 1606 2813 3777 4454 5602 5847 1606 Size: 1207 964 677 1148 245 2120 Fragments arranged by size: 2120 1207 1148 964 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4870 Fragments arranged by size: 4870 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NsiI A_TGCA'T Cuts at: 3001 3001 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 2957 3073 3719 5264 1132 Size: 97 1728 116 646 1545 2229 Fragments arranged by size: 2229 1728 1545 646 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6361 PciI A'CATG_T Cuts at: 2953 3069 5260 2953 Size: 116 2191 4054 Fragments arranged by size: 4054 2191 116 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6361 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4430 4462 5943 5975 4430 Size: 32 1481 32 4816 Fragments arranged by size: 4816 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6361 PsiI TTA'TAA Cuts at: 2778 3931 2778 Size: 1153 5208 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3617 3649 3617 Size: 32 6329 PstI C_TGCA'G Cuts at: 830 3713 830 Size: 2883 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 3271 4609 6321 3271 Size: 1338 1712 3311 Fragments arranged by size: 3311 1712 1338 SfcI C'TryA_G Cuts at: 826 1045 3709 4844 5525 5716 826 Size: 219 2664 1135 681 191 1471 Fragments arranged by size: 2664 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3688 1872 Size: 1816 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3097 3376 4465 5366 5628 820 Size: 197 926 1154 279 1089 901 262 1553 Fragments arranged by size: 1553 1154 1089 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SspI AAT'ATT Cuts at: 5 52 3211 4285 5 Size: 47 3159 1074 2081 Fragments arranged by size: 3159 2081 1074 47 StuI AGG'CCT Cuts at: 1517 2199 1517 Size: 682 5679 StyI C'CwwG_G Cuts at: 513 1065 2004 2396 3025 3800 513 Size: 552 939 392 629 775 3074 Fragments arranged by size: 3074 939 775 629 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4388 4405 4558 4743 5188 325 Size: 1255 2808 17 153 185 445 1498 Fragments arranged by size: 2808 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2416 3130 3269 Size: 274 80 33 51 1443 439 714 139 Cuts at: 3269 4607 6319 96 Size: 1338 1712 138 Fragments arranged by size: 1712 1443 1338 714 439 274 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2250 2282 6321 6353 2250 Size: 32 4039 32 2258 Fragments arranged by size: 4039 2258 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2064 1316 Size: 748 5613 XbaI T'CTAG_A Cuts at: 3475 3697 3475 Size: 222 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2975 3307 2975 Size: 332 6029 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6361 XmaI C'CCGG_G Cuts at: 1870 3686 1870 Size: 1816 4545 XmnI GAAnn'nnTTC Cuts at: 4490 4490 Size: 6361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI