Restriction map for
pFN21AA0864
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AscI AseI AsnI Asp718I
AspI BalI BclI BfrI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NsiI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 2950 TCCGGA T^CCGGA
AgeI 191 ACCGGT A^CCGGT
AhdI 1120 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1781 AGCGCT AGC^GCT
ApaI 970 GGGCCC GGGCC^C
Asp700I 69 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1120 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1835 TGCGCA TGC^GCA
AvrII 1024 CCTAGG C^CTAGG
BamHI 1421 GGATCC G^GATCC
BbeI 2417 GGCGCC GGCGC^C
BciVI 1329 GTATCC GTATCC(6/5)
BglII 2220 AGATCT A^GATCT
BlnI 1024 CCTAGG C^CTAGG
BlpI 2841 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2841 GCTCAGC GC^T[ACGT]AGC
BseAI 2950 TCCGGA T^CCGGA
BsiEI 756 CGACCG CG[GA][CT]^CG
BsmI 2499 GAATGC GAATGC(1/-1)
BspEI 2950 TCCGGA T^CCGGA
BspHI 1433 TCATGA T^CATGA
BsrDI 2188 GCAATG GCAATG(2/0)
CelII 2841 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1120 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1781 AGCGCT AGC^GCT
FspI 1835 TGCGCA TGC^GCA
HincII 2392 GTCAAC GT[CT]^[GA]AC
HindII 2392 GTCAAC GT[CT]^[GA]AC
KasI 2417 GGCGCC G^GCGCC
MroI 2950 TCCGGA T^CCGGA
NarI 2417 GGCGCC GG^CGCC
NruI 2849 TCGCGA TCG^CGA
NspI 1309 GCATGT [GA]CATG^[CT]
PaeR7I 2035 CTCGAG C^TCGAG
PinAI 191 ACCGGT A^CCGGT
RcaI 1433 TCATGA T^CATGA
SbfI 2097 CCTGCAGG CCTGCA^GG
ScaI 2477 AGTACT AGT^ACT
SfiI 331 GGCCGCACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2417 GGCGCC GGC^GCC
Sse8387I 2097 CCTGCAGG CCTGCA^GG
XhoI 2035 CTCGAG C^TCGAG
XmnI 69 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 31 AAATTC [GA]^AATT[CT]
1161 GAATTT
AocI 280 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
ApoI 31 AAATTC [GA]^AATT[CT]
1161 GAATTT
AvaI 1524 CCCGAG C^[CT]CG[GA]G
2035 CTCGAG
BbsI 813 GAAGAC GAAGAC(2/6)
1983 GTCTTC
BbvCI 1267 GCTGAGG CCTCAGC(-5/-2)
2109 GCTGAGG
BcgI 189 GCACCGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1778 GCAAGCGCTTCG
BpuAI 813 GAAGAC GAAGAC(2/6)
1983 GTCTTC
BsgI 24 GTGCAG GTGCAG(16/14)
2446 GTGCAG
BsoBI 1524 CCCGAG C^[CT]CG[GA]G
2035 CTCGAG
BssSI 1705 CACGAG CACGAG(-5/-1)
2017 CACGAG
BstAPI 2439 GCAGTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2535 GCAGGCCCTGC
BstEII 1999 GGTGACC G^GT[ACGT]ACC
2669 GGTCACC
BstPI 1999 GGTGACC G^GT[ACGT]ACC
2669 GGTCACC
Bsu36I 280 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
DsaI 1130 CCACGG C^C[GA][CT]GG
2942 CCACGG
Eco81I 280 CCTCAGG CC^T[ACGT]AGG
1720 CCTGAGG
EcoO65I 1999 GGTGACC G^GT[ACGT]ACC
2669 GGTCACC
SacI 2569 GAGCTC GAGCT^C
2839 GAGCTC
SapI 1103 GAAGAGC GCTCTTC(1/4)
2364 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
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AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaII BanI BanII BbrPI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII