(Circular) (Six-base) MAPSORT of: pFN21AA0864.seq Check: 1834 from: 1 to: 7819 pFN21AA0864 7819 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3952 3952 Size: 7819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5629 278 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5162 1937 Size: 3225 4594 Acc65I G'GTAC_C Cuts at: 5140 5140 Size: 7819 AclI AA'CG_TT Cuts at: 1563 5946 6319 1563 Size: 4383 373 3063 Fragments arranged by size: 4383 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2862 4014 5075 5882 7266 7534 1632 Size: 1230 1152 1061 807 1384 268 1917 Fragments arranged by size: 1917 1384 1230 1152 1061 807 268 AfeI AGC'GCT Cuts at: 3788 3788 Size: 7819 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7622 AflIII A'CryG_T Cuts at: 1203 6718 7695 1203 Size: 5515 977 1327 Fragments arranged by size: 5515 1327 977 AgeI A'CCGG_T Cuts at: 2196 6650 2196 Size: 4454 3365 AhdI GACnn_n'nnGTC Cuts at: 3130 6548 3130 Size: 3418 4401 AleI CACnn'nnGTG Cuts at: 1166 2318 1166 Size: 1152 6667 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7819 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4452 4484 4452 Size: 32 7787 AlwNI CAG_nnn'CTG Cuts at: 2854 3272 3667 3824 4432 4525 4546 4597 4750 Size: 418 395 157 608 93 21 51 153 Cuts at: 4750 5013 5562 7134 2854 Size: 263 549 1572 3539 Fragments arranged by size: 3539 1572 608 549 418 395 263 157 153 93 51 21 ApaI G_GGCC'C Cuts at: 2979 2979 Size: 7819 ApaLI G'TGCA_C Cuts at: 2192 2311 3449 3759 5876 7032 2192 Size: 119 1138 310 2117 1156 2979 Fragments arranged by size: 2979 2117 1156 1138 310 119 ApoI r'AATT_y Cuts at: 1493 2036 3166 5128 5354 5506 7615 1493 Size: 543 1130 1962 226 152 2109 1697 Fragments arranged by size: 2109 1962 1697 1130 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 6373 160 Size: 872 5341 1606 Fragments arranged by size: 5341 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 AvaI C'yCGr_G Cuts at: 1870 1943 3529 4040 5144 1870 Size: 73 1586 511 1104 4545 Fragments arranged by size: 4545 1586 1104 511 73 AvrII C'CTAG_G Cuts at: 3029 3029 Size: 7819 BamHI G'GATC_C Cuts at: 3426 5149 5516 3426 Size: 1723 367 5729 Fragments arranged by size: 5729 1723 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 3964 4422 5140 6595 Size: 325 217 627 504 1673 458 718 1455 Cuts at: 6595 618 Size: 1842 Fragments arranged by size: 1842 1673 1455 718 627 504 458 325 217 BanII G_rGCy'C Cuts at: 1391 2496 2979 3144 3717 3863 4578 4848 5138 Size: 1105 483 165 573 146 715 270 290 Cuts at: 5138 1391 Size: 4072 Fragments arranged by size: 4072 1105 715 573 483 290 270 165 146 BbeI G_GCGC'C Cuts at: 4426 4426 Size: 7819 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 3981 928 Size: 971 926 1156 4766 Fragments arranged by size: 4766 1156 971 926 BbvCI CC'TCA_GC Cuts at: 3273 4115 3273 Size: 842 6977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2183 2217 3772 3806 6010 6044 1903 Size: 34 246 34 1555 34 2204 34 3678 Fragments arranged by size: 3678 2204 1555 246 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3345 5711 6921 3345 Size: 2366 1210 4243 Fragments arranged by size: 4243 2366 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2343 2528 Size: 107 122 71 631 605 337 334 185 Cuts at: 2528 4514 6430 136 Size: 1986 1916 1525 Fragments arranged by size: 1986 1916 1525 631 605 337 334 185 122 107 71 BglII A'GATC_T Cuts at: 4225 7814 4225 Size: 3589 4230 BlpI GC'TnA_GC Cuts at: 4847 5236 4847 Size: 389 7430 Bme1580I G_kGCm'C Cuts at: 1790 2196 2294 2315 2979 3453 3763 3969 5880 Size: 406 98 21 664 474 310 206 1911 Cuts at: 5880 7036 1790 Size: 1156 2573 Fragments arranged by size: 2573 1911 1156 664 474 406 310 206 98 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2183 2571 3646 Size: 416 53 83 399 24 766 388 1075 Cuts at: 3646 4656 4680 4931 5550 6508 442 Size: 1010 24 251 619 958 1753 Fragments arranged by size: 1753 1075 1010 958 766 619 416 399 388 251 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7819 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2685 2709 3637 4048 4489 4501 4783 2685 Size: 24 928 411 441 12 282 5721 Fragments arranged by size: 5721 928 441 411 282 24 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2148 2450 2834 3767 4073 4106 4214 4385 Size: 909 302 384 933 306 33 108 171 Cuts at: 4385 4454 4484 4520 4547 6479 1239 Size: 69 30 36 27 1932 2579 Fragments arranged by size: 2579 1932 933 909 384 306 302 171 108 69 36 33 30 27 Bpu10I CC'TnA_GC Cuts at: 3273 3576 3672 4115 4863 3273 Size: 303 96 443 748 6229 Fragments arranged by size: 6229 748 443 303 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4757 4886 5944 6809 7107 4757 Size: 129 1058 865 298 5469 Fragments arranged by size: 5469 1058 865 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 2396 2954 3515 3929 6482 882 Size: 617 706 191 558 561 414 2553 2219 Fragments arranged by size: 2553 2219 706 617 561 558 414 191 BsaAI yAC'GTr Cuts at: 493 1204 3448 3904 4159 4173 5610 493 Size: 711 2244 456 255 14 1437 2702 Fragments arranged by size: 2702 2244 1437 711 456 255 14 BsaBI GATnn'nnATC Cuts at: 7813 7813 Size: 7819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2372 3078 3152 3804 Size: 53 83 186 936 839 706 74 652 Cuts at: 3804 4423 5626 6008 6643 275 Size: 619 1203 382 635 1451 Fragments arranged by size: 1451 1203 936 839 706 652 635 619 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 2196 3855 4955 6252 6650 6924 7071 7482 2196 Size: 1659 1100 1297 398 274 147 411 2533 Fragments arranged by size: 2533 1659 1297 1100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 3368 3398 3998 Size: 30 165 30 609 30 1326 30 600 Cuts at: 3998 4028 4430 4460 1178 Size: 30 402 30 4537 Fragments arranged by size: 4537 1326 609 600 402 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2756 3057 3290 3601 3788 4307 4451 1186 Size: 1570 301 233 311 187 519 144 4554 Fragments arranged by size: 4554 1570 519 311 301 233 187 144 BseYI C'CCAG_C Cuts at: 2678 2876 2882 3061 3559 7022 7518 2678 Size: 198 6 179 498 3463 496 2979 Fragments arranged by size: 3463 2979 498 496 198 179 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2050 4472 1163 Size: 573 314 2422 4510 Fragments arranged by size: 4510 2422 573 314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 5269 6030 7058 1540 Size: 275 186 763 2505 761 1028 2301 Fragments arranged by size: 2505 2301 1028 763 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2241 2315 2955 3453 3763 4578 4848 5880 Size: 45 74 640 498 310 815 270 1032 Cuts at: 5880 5965 7036 2196 Size: 85 1071 2979 Fragments arranged by size: 2979 1071 1032 815 640 498 310 270 85 74 45 BsmI GAATG_Cn' Cuts at: 4510 5330 5423 4510 Size: 820 93 6906 Fragments arranged by size: 6906 820 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2241 2294 2315 2496 2955 2979 Size: 399 406 45 53 21 181 459 24 Cuts at: 2979 3144 3453 3717 3763 3863 3969 4578 4848 Size: 165 309 264 46 100 106 609 270 Cuts at: 4848 5138 5880 5965 7036 1391 Size: 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 459 406 399 309 290 270 264 181 165 106 100 85 53 46 45 24 21 BspEI T'CCGG_A Cuts at: 4955 7482 4955 Size: 2527 5292 BspHI T'CATG_A Cuts at: 3438 5708 7438 3438 Size: 2270 1730 3819 Fragments arranged by size: 3819 2270 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3168 3533 3952 4875 5174 844 Size: 2324 365 419 923 299 3489 Fragments arranged by size: 3489 2324 923 419 365 299 BsrBI CCG'CTC Cuts at: 1394 1823 2799 2866 3604 3621 3679 3751 4357 Size: 429 976 67 738 17 58 72 606 Cuts at: 4357 4537 5706 1394 Size: 180 1169 3507 Fragments arranged by size: 3507 1169 976 738 606 429 180 72 67 58 17 BsrDI GCAATG_nn' Cuts at: 65 1248 4200 6314 6488 65 Size: 1183 2952 2114 174 1396 Fragments arranged by size: 2952 2114 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 4008 4095 6463 6650 1700 Size: 110 386 1812 87 2368 187 2869 Fragments arranged by size: 2869 2368 1812 386 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7819 BssSI C'ACGA_G Cuts at: 3710 4022 5879 6891 3710 Size: 312 1857 1012 4638 Fragments arranged by size: 4638 1857 1012 312 BstAPI GCAn_nnn'nTGC Cuts at: 794 4450 4546 794 Size: 3656 96 4067 Fragments arranged by size: 4067 3656 96 BstBI TT'CG_AA Cuts at: 6623 6623 Size: 7819 BstEII G'GTnAC_C Cuts at: 1184 4004 4674 1184 Size: 2820 670 4329 Fragments arranged by size: 4329 2820 670 BstXI CCAn_nnnn'nTGG Cuts at: 2648 2971 3121 2648 Size: 323 150 7346 Fragments arranged by size: 7346 323 150 BstYI r'GATC_y Cuts at: 1972 3426 4225 5009 5149 5516 5901 5918 7370 Size: 1454 799 784 140 367 385 17 1452 Cuts at: 7370 7456 7468 7814 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1454 1452 799 784 385 367 346 140 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2286 3726 2286 Size: 1440 6379 BtgI C'CryG_G Cuts at: 513 1065 3135 4947 513 Size: 552 2070 1812 3385 Fragments arranged by size: 3385 2070 1812 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3955 4837 487 Size: 683 56 2729 882 3469 Fragments arranged by size: 3469 2729 882 683 56 BtsI GCAGTG_nn' Cuts at: 2976 3459 4559 4793 5335 6129 6149 2976 Size: 483 1100 234 542 794 20 4646 Fragments arranged by size: 4646 1100 794 542 483 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7784 DraI TTT'AAA Cuts at: 5123 5123 Size: 7819 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6826 809 Size: 507 5510 1802 Fragments arranged by size: 5510 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2776 3205 3396 Size: 54 1425 50 275 18 946 429 191 Cuts at: 3396 4302 5266 6155 7784 8 Size: 906 964 889 1629 43 Fragments arranged by size: 1629 1425 964 946 906 889 429 275 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5266 1537 Size: 275 3454 4090 Fragments arranged by size: 4090 3454 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2733 3011 3103 3578 3948 4263 4364 5749 Size: 1380 278 92 475 370 315 101 1385 Cuts at: 5749 1353 Size: 3423 Fragments arranged by size: 3423 1385 1380 475 370 315 278 101 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2414 3433 3746 6406 6780 6926 Size: 1064 138 825 1019 313 2660 374 146 Cuts at: 6926 387 Size: 1280 Fragments arranged by size: 2660 1280 1064 1019 825 374 313 146 138 EcoICRI GAG'CTC Cuts at: 4576 4846 4576 Size: 270 7549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2450 2834 2862 3767 4014 4073 Size: 393 516 302 384 28 905 247 59 Cuts at: 4073 4106 4214 4385 4454 4484 4520 4547 5075 Size: 33 108 171 69 30 36 27 528 Cuts at: 5075 5882 6479 7266 7534 1239 Size: 807 597 787 268 1524 Fragments arranged by size: 1524 905 807 787 597 528 516 393 384 302 268 247 171 108 69 59 36 33 30 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2285 3529 3671 4289 4685 4754 2123 Size: 162 1244 142 618 396 69 5188 Fragments arranged by size: 5188 1244 618 396 162 142 69 EcoO109I rG'GnC_Cy Cuts at: 2116 2904 2975 2976 3664 3695 4414 4468 4543 Size: 788 71 1 688 31 719 54 75 Cuts at: 4543 2116 Size: 5392 Fragments arranged by size: 5392 788 719 688 75 71 54 31 1 EcoRI G'AATT_C Cuts at: 5128 5506 5128 Size: 378 7441 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3058 3058 Size: 7819 FspI TGC'GCA Cuts at: 3842 6325 3842 Size: 2483 5336 HaeII r_GCGC'y Cuts at: 1678 2751 3781 3790 4146 4426 4526 6966 1678 Size: 1073 1030 9 356 280 100 2440 2531 Fragments arranged by size: 2531 2440 1073 1030 356 280 100 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 2269 2301 3116 Size: 32 135 32 481 32 184 32 815 Cuts at: 3116 3148 3928 3960 3998 4030 4210 4242 4430 Size: 32 780 32 38 32 180 32 188 Cuts at: 4430 4462 5001 5033 6460 6492 6534 6566 1373 Size: 32 539 32 1427 32 42 32 2626 Fragments arranged by size: 2626 1427 815 780 539 481 188 184 180 135 42 38 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4399 5163 5409 669 Size: 988 281 2461 764 246 3079 Fragments arranged by size: 3079 2461 988 764 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7819 HpaI GTT'AAC Cuts at: 5409 5409 Size: 7819 KasI G'GCGC_C Cuts at: 4422 4422 Size: 7819 KpnI G_GTAC'C Cuts at: 5144 5144 Size: 7819 MfeI C'AATT_G Cuts at: 5418 5418 Size: 7819 MluI A'CGCG_T Cuts at: 7695 7695 Size: 7819 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6933 7117 1305 Size: 5628 184 2007 Fragments arranged by size: 5628 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5997 Fragments arranged by size: 5997 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2318 3848 5777 6136 6295 518 Size: 648 1134 18 1530 1929 359 159 2042 Fragments arranged by size: 2042 1929 1530 1134 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2854 3510 3919 4114 4246 4301 4378 4456 Size: 1248 656 409 195 132 55 77 78 Cuts at: 4456 5235 5912 7060 7305 1606 Size: 779 677 1148 245 2120 Fragments arranged by size: 2120 1248 1148 779 677 656 409 245 195 132 78 77 55 NarI GG'CG_CC Cuts at: 4423 4423 Size: 7819 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7267 NdeI CA'TA_TG Cuts at: 387 387 Size: 7819 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7819 NotI GC'GGCC_GC Cuts at: 5266 5266 Size: 7819 NruI TCG'CGA Cuts at: 4856 4856 Size: 7819 NspI r_CATG'y Cuts at: 1132 1229 3318 5177 6722 1132 Size: 97 2089 1859 1545 2229 Fragments arranged by size: 2229 2089 1859 1545 97 PasI CC'CwG_GG Cuts at: 1348 3863 4126 1348 Size: 2515 263 5041 Fragments arranged by size: 5041 2515 263 PciI A'CATG_T Cuts at: 6718 6718 Size: 7819 PflMI CCAn_nnn'nTGG Cuts at: 1108 2613 4432 4525 1108 Size: 1505 1819 93 4402 Fragments arranged by size: 4402 1819 1505 93 PfoI T'CCnGG_A Cuts at: 3948 3948 Size: 7819 PmeI GTTT'AAAC Cuts at: 5123 5123 Size: 7819 PmlI CAC'GTG Cuts at: 3448 3904 4173 3448 Size: 456 269 7094 Fragments arranged by size: 7094 456 269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5888 5920 7401 7433 5888 Size: 32 1481 32 6274 Fragments arranged by size: 6274 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 2904 4468 2116 Size: 788 1564 5467 Fragments arranged by size: 5467 1564 788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7819 PsiI TTA'TAA Cuts at: 5389 5389 Size: 7819 PspOMI G'GGCC_C Cuts at: 2975 2975 Size: 7819 PstI C_TGCA'G Cuts at: 830 4107 4167 4446 4618 4788 5171 830 Size: 3277 60 279 172 170 383 3478 Fragments arranged by size: 3478 3277 383 279 172 170 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7819 PvuII CAG'CTG Cuts at: 2854 3510 4114 2854 Size: 656 604 6559 Fragments arranged by size: 6559 656 604 SacI G_AGCT'C Cuts at: 4578 4848 4578 Size: 270 7549 SalI G'TCGA_C Cuts at: 1936 5161 1936 Size: 3225 4594 SapI GCTCTTCn'nnn_ Cuts at: 3103 4364 3103 Size: 1261 6558 SbfI CC_TGCA'GG Cuts at: 4107 5171 4107 Size: 1064 6755 ScaI AGT'ACT Cuts at: 4484 6067 7779 4484 Size: 1583 1712 4524 Fragments arranged by size: 4524 1712 1583 SfcI C'TryA_G Cuts at: 826 1045 3256 4103 4163 4442 4614 4784 5167 Size: 219 2211 847 60 279 172 170 383 Cuts at: 5167 6302 6983 7174 826 Size: 1135 681 191 1471 Fragments arranged by size: 2211 1471 1135 847 681 383 279 219 191 172 170 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2343 2343 Size: 7819 SfoI GGC'GCC Cuts at: 4424 4424 Size: 7819 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7819 SmaI CCC'GGG Cuts at: 1872 5146 1872 Size: 3274 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4040 4772 4901 5923 6824 7086 Size: 197 926 2097 732 129 1022 901 262 Cuts at: 7086 820 Size: 1553 Fragments arranged by size: 2097 1553 1022 926 901 732 262 197 129 SnaBI TAC'GTA Cuts at: 493 5610 493 Size: 5117 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7819 SphI G_CATG'C Cuts at: 1132 5177 1132 Size: 4045 3774 SspI AAT'ATT Cuts at: 5 52 5743 5 Size: 47 5691 2081 Fragments arranged by size: 5691 2081 47 StuI AGG'CCT Cuts at: 1517 2930 3535 4073 5064 1517 Size: 1413 605 538 991 4272 Fragments arranged by size: 4272 1413 991 605 538 StyI C'CwwG_G Cuts at: 513 1065 2016 2406 2523 2634 2931 3029 5258 Size: 552 951 390 117 111 297 98 2229 Cuts at: 5258 513 Size: 3074 Fragments arranged by size: 3074 2229 951 552 390 297 117 111 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2412 3722 5846 5863 6016 6201 Size: 1255 609 223 1310 2124 17 153 185 Cuts at: 6201 6646 325 Size: 445 1498 Fragments arranged by size: 2124 1498 1310 1255 609 445 223 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4482 4824 6065 Size: 274 80 33 51 1443 2505 342 1241 Cuts at: 6065 7777 96 Size: 1712 138 Fragments arranged by size: 2505 1712 1443 1241 342 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 2266 2298 2888 2920 3194 3226 3319 Size: 32 222 32 590 32 274 32 93 Cuts at: 3319 3351 7779 7811 2012 Size: 32 4428 32 2020 Fragments arranged by size: 4428 2020 590 274 222 93 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7819 XbaI T'CTAG_A Cuts at: 5155 5155 Size: 7819 XhoI C'TCGA_G Cuts at: 1943 4040 1943 Size: 2097 5722 XmaI C'CCGG_G Cuts at: 1870 5144 1870 Size: 3274 4545 XmnI GAAnn'nnTTC Cuts at: 2078 5948 2078 Size: 3870 3949 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5627 276 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI