Restriction map for
pFN21AA0853
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI ApaI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BfrI BglI BlnI
BlpI Bpu1102I Bsp1407I BsrGI BssHII
Bst1107I BstZ17I Bsu36I CelII CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoT22I FseI FspI HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NcoI
NdeI NotI NruI NsiI PacI
PflFI PinAI PmeI PpuMI PshAI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfoI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 3931 CGCG CG^CG
AccIII 2535 TCCGGA T^CCGGA
Alw44I 2798 GTGCAC G^TGCAC
Aor51HI 2471 AGCGCT AGC^GCT
ApaLI 2798 GTGCAC G^TGCAC
BalI 3991 TGGCCA TGG^CCA
BanI 4837 GGCACC G^G[CT][GA]CC
BbrPI 3514 CACGTG CAC^GTG
BcgI 3566 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1577 CCCGG CC^[GC]GG
BsaWI 2535 TCCGGA [AT]^CCGG[AT]
BseAI 2535 TCCGGA T^CCGGA
BsiEI 2199 CGATCG CG[GA][CT]^CG
BsiWI 1618 CGTACG C^GTACG
BspDI 3908 ATCGAT AT^CGAT
BspEI 2535 TCCGGA T^CCGGA
BsrDI 3137 GCAATG GCAATG(2/0)
BsrFI 2519 GCCGGC [GA]^CCGG[CT]
BstBI 4808 TTCGAA TT^CGAA
BstEII 227 GGTCACC G^GT[ACGT]ACC
BstPI 227 GGTCACC G^GT[ACGT]ACC
BstUI 3931 CGCG CG^CG
BstXI 3642 CCATCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2519 GCCGGC [GA]^CCGG[CT]
ClaI 3908 ATCGAT AT^CGAT
DraIII 191 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3091 CCACGG C^C[GA][CT]GG
Eco47III 2471 AGCGCT AGC^GCT
EcoO65I 227 GGTCACC G^GT[ACGT]ACC
HindIII 4850 AAGCTT A^AGCTT
MluNI 3991 TGGCCA TGG^CCA
MroI 2535 TCCGGA T^CCGGA
MscI 3991 TGGCCA TGG^CCA
MvnI 3931 CGCG CG^CG
NaeI 2519 GCCGGC GCC^GGC
NciI 1577 CCCGG CC^[GC]GG
NgoMIV 2519 GCCGGC G^CCGGC
NheI 688 GCTAGC G^CTAGC
NspV 4808 TTCGAA TT^CGAA
PaeR7I 1355 CTCGAG C^TCGAG
PmaCI 3514 CACGTG CAC^GTG
PmlI 3514 CACGTG CAC^GTG
PstI 713 CTGCAG CTGCA^G
PvuI 2199 CGATCG CGAT^CG
PvuII 1782 CAGCTG CAG^CTG
SapI 1322 GCTCTTC GCTCTTC(1/4)
SfuI 4808 TTCGAA TT^CGAA
SgrAI 2518 CGCCGGCG C[GA]^CCGG[CT]G
XcmI 3858 CCATTCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1355 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 1235 GACGCC G[GA]^CG[CT]C
3507 GACGCC
AflIII 166 ACATGT A^C[GA][CT]GT
4642 ACATGT
AseI 466 ATTAAT AT^TAAT
528 ATTAAT
AsnI 466 ATTAAT AT^TAAT
528 ATTAAT
BclI 1203 TGATCA T^GATCA
3753 TGATCA
BglII 3865 AGATCT A^GATCT
4257 AGATCT
BsaAI 782 CACGTA [CT]AC^GT[GA]
3514 CACGTG
BsaHI 1235 GACGCC G[GA]^CG[CT]C
3507 GACGCC
BsmBI 1139 CGTCTC CGTCTC(1/5)
3505 GAGACG
BsmI 2792 GAATGC GAATGC(1/-1)
3187 GCATTC
BspLU11I 166 ACATGT A^CATGT
4642 ACATGT
BspMI 4420 GCAGGT ACCTGC(4/8)
4609 GCAGGT
BsrBI 2062 GAGCGG CCGCTC(-3/-3)
2206 GAGCGG
BstAPI 3349 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4420 GCAGGTGATGC
EaeI 1587 TGGCCG [CT]^GGCC[GA]
3991 TGGCCA
EcoRV 817 GATATC GAT^ATC
1820 GATATC
EcoT14I 940 CCAAGG C^C[AT][AT]GG
4232 CCTTGG
FbaI 1203 TGATCA T^GATCA
3753 TGATCA
Hin1I 1235 GACGCC G[GA]^CG[CT]C
3507 GACGCC
MspA1I 1078 CAGCGG C[AC]G^C[GT]G
1782 CAGCTG
PflMI 279 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2437 CCAAGAGATGG
PshBI 466 ATTAAT AT^TAAT
528 ATTAAT
SacI 1320 GAGCTC GAGCT^C
2852 GAGCTC
ScaI 3678 AGTACT AGT^ACT
3784 AGTACT
StyI 940 CCAAGG C^C[AT][AT]GG
4232 CCTTGG
Van91I 279 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2437 CCAAGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI