(Circular) (Six-base) MAPSORT of: pFN21AA0853.seq Check: 8188 from: 1 to: 9709 pFN21AA0853 9709 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6416 6416 Size: 9709 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7519 278 Size: 53 83 186 936 5983 2468 Fragments arranged by size: 5983 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 3999 6879 7052 1937 Size: 1014 1048 2880 173 4594 Fragments arranged by size: 4594 2880 1048 1014 173 Acc65I G'GTAC_C Cuts at: 7030 7030 Size: 9709 AclI AA'CG_TT Cuts at: 1563 7836 8209 1563 Size: 6273 373 3063 Fragments arranged by size: 6273 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2673 4854 4983 5781 6778 6978 7772 Size: 530 511 2181 129 798 997 200 794 Cuts at: 7772 9156 9424 1632 Size: 1384 268 1917 Fragments arranged by size: 2181 1917 1384 997 798 794 530 511 268 200 129 AfeI AGC'GCT Cuts at: 4478 4478 Size: 9709 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9512 AflIII A'CryG_T Cuts at: 1203 2171 6647 8608 9585 1203 Size: 968 4476 1961 977 1327 Fragments arranged by size: 4476 1961 1327 977 968 AgeI A'CCGG_T Cuts at: 8540 8540 Size: 9709 AhdI GACnn_n'nnGTC Cuts at: 8438 8438 Size: 9709 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9709 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4690 4722 4690 Size: 32 9677 AlwNI CAG_nnn'CTG Cuts at: 5202 6620 6997 7452 9024 5202 Size: 1418 377 455 1572 5887 Fragments arranged by size: 5887 1572 1418 455 377 ApaLI G'TGCA_C Cuts at: 4803 7766 8922 4803 Size: 2963 1156 5590 Fragments arranged by size: 5590 2963 1156 ApoI r'AATT_y Cuts at: 1493 2752 4887 6417 6563 7018 7244 7396 9505 Size: 1259 2135 1530 146 455 226 152 2109 Cuts at: 9505 1493 Size: 1697 Fragments arranged by size: 2135 2109 1697 1530 1259 455 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 2472 2534 8263 160 Size: 872 1440 62 5729 1606 Fragments arranged by size: 5729 1606 1440 872 62 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9709 AvaI C'yCGr_G Cuts at: 1870 1943 3360 5579 6222 7034 1870 Size: 73 1417 2219 643 812 4545 Fragments arranged by size: 4545 2219 1417 812 643 73 BamHI G'GATC_C Cuts at: 7039 7406 7039 Size: 367 9342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6842 7030 8485 618 Size: 325 217 627 5055 188 1455 1842 Fragments arranged by size: 5055 1842 1455 627 325 217 188 BanII G_rGCy'C Cuts at: 1391 2304 3329 4861 6712 7028 1391 Size: 913 1025 1532 1851 316 4072 Fragments arranged by size: 4072 1851 1532 1025 913 316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2077 2150 2385 2679 2717 3883 4383 Size: 971 178 73 235 294 38 1166 500 Cuts at: 4383 4659 5472 5612 6746 6896 6929 928 Size: 276 813 140 1134 150 33 3708 Fragments arranged by size: 3708 1166 1134 971 813 500 294 276 235 178 150 140 73 38 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5560 5594 7900 7934 1903 Size: 34 3623 34 2306 34 3678 Fragments arranged by size: 3678 3623 2306 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3556 6377 6995 7601 8811 3556 Size: 2821 618 606 1210 4454 Fragments arranged by size: 4454 2821 1210 618 606 BclI T'GATC_A Cuts at: 1548 3208 5758 1548 Size: 1660 2550 5499 Fragments arranged by size: 5499 2550 1660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8320 136 Size: 107 122 71 631 605 6648 1525 Fragments arranged by size: 6648 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 6262 9704 5870 Size: 392 3442 5875 Fragments arranged by size: 5875 3442 392 BlpI GC'TnA_GC Cuts at: 7126 7126 Size: 9709 Bme1580I G_kGCm'C Cuts at: 1790 4807 6845 7770 8926 1790 Size: 3017 2038 925 1156 2573 Fragments arranged by size: 3017 2573 2038 1156 925 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 8737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2070 5775 7440 Size: 416 53 83 399 24 653 3705 1665 Cuts at: 7440 8398 442 Size: 958 1753 Fragments arranged by size: 3705 1753 1665 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2697 1056 Size: 1641 8068 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 2347 Size: 9709 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2391 3406 5826 6396 6594 6924 8369 1239 Size: 1152 1015 2420 570 198 330 1445 2579 Fragments arranged by size: 2579 2420 1445 1152 1015 570 330 198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3277 3424 3721 3986 4599 4615 4718 4777 5683 Size: 147 297 265 613 16 103 59 906 Cuts at: 5683 7834 8699 8997 3277 Size: 2151 865 298 3989 Fragments arranged by size: 3989 2151 906 865 613 298 297 265 147 103 59 16 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3330 3885 4185 4191 8372 882 Size: 617 1831 555 300 6 4181 2219 Fragments arranged by size: 4181 2219 1831 617 555 300 6 BsaAI yAC'GTr Cuts at: 493 1204 2789 5521 7500 493 Size: 711 1585 2732 1979 2702 Fragments arranged by size: 2732 2702 1979 1585 711 BsaBI GATnn'nnATC Cuts at: 3549 3567 3825 4437 6261 9703 3549 Size: 18 258 612 1824 3442 3555 Fragments arranged by size: 3555 3442 1824 612 258 18 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3241 5513 7516 7898 Size: 53 83 186 936 1708 2272 2003 382 Cuts at: 7898 8533 275 Size: 635 1451 Fragments arranged by size: 2272 2003 1708 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4540 8142 8540 8814 8961 9372 4540 Size: 3602 398 274 147 411 4877 Fragments arranged by size: 4877 3602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3079 3109 5272 5302 5584 Size: 30 165 30 1676 30 2163 30 282 Cuts at: 5584 5614 6853 6883 1178 Size: 30 1239 30 4004 Fragments arranged by size: 4004 2163 1676 1239 282 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 3039 4691 5280 5301 5581 5962 6476 Size: 1150 703 1652 589 21 280 381 514 Cuts at: 6476 1186 Size: 4419 Fragments arranged by size: 4419 1652 1150 703 589 514 381 280 21 BseYI C'CCAG_C Cuts at: 2099 2959 3789 4499 5070 8912 9408 2099 Size: 860 830 710 571 3842 496 2400 Fragments arranged by size: 3842 2400 860 830 710 571 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5672 6296 6537 1163 Size: 573 3936 624 241 4335 Fragments arranged by size: 4335 3936 624 573 241 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4207 7159 7920 8948 1540 Size: 275 186 2206 2952 761 1028 2301 Fragments arranged by size: 2952 2301 2206 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3329 4807 4861 6871 7770 7855 8926 3329 Size: 1478 54 2010 899 85 1071 4112 Fragments arranged by size: 4112 2010 1478 1071 899 85 54 BsiWI C'GTAC_G Cuts at: 3623 3623 Size: 9709 BsmI GAATG_Cn' Cuts at: 4803 5192 7220 7313 4803 Size: 389 2028 93 7199 Fragments arranged by size: 7199 2028 389 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3150 5504 3150 Size: 2354 7355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 3329 4807 4861 6712 6845 6871 Size: 399 514 1025 1478 54 1851 133 26 Cuts at: 6871 7028 7770 7855 8926 1391 Size: 157 742 85 1071 2174 Fragments arranged by size: 2174 1851 1478 1071 1025 742 514 399 157 133 85 54 26 BspEI T'CCGG_A Cuts at: 4540 9372 4540 Size: 4832 4877 BspHI T'CATG_A Cuts at: 2488 3211 3232 3781 4861 7598 9328 2488 Size: 723 21 549 1080 2737 1730 2869 Fragments arranged by size: 2869 2737 1730 1080 723 549 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6416 6605 7064 844 Size: 5572 189 459 3489 Fragments arranged by size: 5572 3489 459 189 BsrBI CCG'CTC Cuts at: 1394 1823 4069 4213 7596 1394 Size: 429 2246 144 3383 3507 Fragments arranged by size: 3507 3383 2246 429 144 BsrDI GCAATG_nn' Cuts at: 65 1248 5149 8204 8378 65 Size: 1183 3901 3055 174 1396 Fragments arranged by size: 3901 3055 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4524 8353 8540 1700 Size: 110 2714 3829 187 2869 Fragments arranged by size: 3829 2869 2714 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9709 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9709 BssSI C'ACGA_G Cuts at: 2199 3507 5493 6537 6862 7769 8781 2199 Size: 1308 1986 1044 325 907 1012 3127 Fragments arranged by size: 3127 1986 1308 1044 1012 907 325 BstAPI GCAn_nnn'nTGC Cuts at: 794 5360 6431 794 Size: 4566 1071 4072 Fragments arranged by size: 4566 4072 1071 BstBI TT'CG_AA Cuts at: 6814 8513 6814 Size: 1699 8010 BstEII G'GTnAC_C Cuts at: 1184 2232 1184 Size: 1048 8661 BstXI CCAn_nnnn'nTGG Cuts at: 5654 5654 Size: 9709 BstYI r'GATC_y Cuts at: 1972 2254 4422 5870 6205 6262 6269 6475 7039 Size: 282 2168 1448 335 57 7 206 564 Cuts at: 7039 7406 7791 7808 9260 9346 9358 9704 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2168 1977 1452 1448 564 385 367 346 335 282 206 86 57 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 5096 513 Size: 552 4031 5126 Fragments arranged by size: 5126 4031 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3161 5528 487 Size: 683 56 1935 2367 4668 Fragments arranged by size: 4668 2367 1935 683 56 BtsI GCAGTG_nn' Cuts at: 5334 7225 8019 8039 5334 Size: 1891 794 20 7004 Fragments arranged by size: 7004 1891 794 20 ClaI AT'CG_AT Cuts at: 1554 5914 1554 Size: 4360 5349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9674 DraI TTT'AAA Cuts at: 7013 7013 Size: 9709 DraIII CAC_nnn'GTG Cuts at: 2201 2201 Size: 9709 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8716 809 Size: 507 7400 1802 Fragments arranged by size: 7400 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3592 5996 7156 Size: 54 1425 50 275 18 1762 2404 1160 Cuts at: 7156 8045 9674 8 Size: 889 1629 43 Fragments arranged by size: 2404 1762 1629 1425 1160 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7156 1537 Size: 275 5344 4090 Fragments arranged by size: 5344 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2558 3334 3976 3988 4260 4413 5307 7639 Size: 1205 776 642 12 272 153 894 2332 Cuts at: 7639 1353 Size: 3423 Fragments arranged by size: 3423 2332 1205 894 776 642 272 153 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8296 8670 8816 387 Size: 1064 138 6707 374 146 1280 Fragments arranged by size: 6707 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3327 4859 3327 Size: 1532 8177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2391 2673 3406 4854 4983 5781 Size: 393 530 229 282 733 1448 129 798 Cuts at: 5781 5826 6396 6594 6778 6924 6978 7772 8369 Size: 45 570 198 184 146 54 794 597 Cuts at: 8369 9156 9424 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1448 798 794 787 733 597 570 530 393 282 268 229 198 184 146 129 54 45 EcoRI G'AATT_C Cuts at: 7018 7396 7018 Size: 378 9331 EcoRV GAT'ATC Cuts at: 2824 3827 2824 Size: 1003 8706 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2970 3084 3879 5811 2970 Size: 114 795 1932 6868 Fragments arranged by size: 6868 1932 795 114 FspI TGC'GCA Cuts at: 8215 8215 Size: 9709 HaeII r_GCGC'y Cuts at: 1678 2284 4480 4525 4717 8856 1678 Size: 606 2196 45 192 4139 2531 Fragments arranged by size: 4139 2531 2196 606 192 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2312 2344 3079 Size: 32 135 32 476 32 232 32 735 Cuts at: 3079 3111 3193 3225 3311 3343 3469 3485 3501 Size: 32 82 32 86 32 126 16 16 Cuts at: 3501 3517 4407 4439 4837 4869 4943 4975 5732 Size: 16 890 32 398 32 74 32 757 Cuts at: 5732 5764 6254 6286 6320 6352 6460 6492 8350 Size: 32 490 32 34 32 108 32 1858 Cuts at: 8350 8382 8424 8456 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1858 890 757 735 490 476 398 232 135 126 108 86 82 74 42 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 7053 7299 669 Size: 988 281 5115 246 3079 Fragments arranged by size: 5115 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 6855 748 Size: 6107 3602 HpaI GTT'AAC Cuts at: 7299 7299 Size: 9709 KpnI G_GTAC'C Cuts at: 7034 7034 Size: 9709 MfeI C'AATT_G Cuts at: 7308 7308 Size: 9709 MluI A'CGCG_T Cuts at: 9585 9585 Size: 9709 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2718 4909 7012 8823 9007 1305 Size: 1413 2191 2103 1811 184 2007 Fragments arranged by size: 2191 2103 2007 1811 1413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5998 10 Size: 54 1425 343 4166 3721 Fragments arranged by size: 4166 3721 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3210 3345 5652 7667 8026 8185 518 Size: 648 2044 135 2307 2015 359 159 2042 Fragments arranged by size: 2307 2044 2042 2015 648 359 159 135 MspA1I CmG'CkG Cuts at: 1606 3085 3789 7125 7802 8950 9195 1606 Size: 1479 704 3336 677 1148 245 2120 Fragments arranged by size: 3336 2120 1479 1148 704 677 245 NaeI GCC'GGC Cuts at: 4526 4526 Size: 9709 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 9157 NdeI CA'TA_TG Cuts at: 387 387 Size: 9709 NgoMIV G'CCGG_C Cuts at: 4524 4524 Size: 9709 NheI G'CTAG_C Cuts at: 1052 2693 1052 Size: 1641 8068 NotI GC'GGCC_GC Cuts at: 7156 7156 Size: 9709 NspI r_CATG'y Cuts at: 1132 1229 2175 3433 6651 7067 8612 1132 Size: 97 946 1258 3218 416 1545 2229 Fragments arranged by size: 3218 2229 1545 1258 946 416 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9709 PciI A'CATG_T Cuts at: 2171 6647 8608 2171 Size: 4476 1961 3272 Fragments arranged by size: 4476 3272 1961 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 4448 1108 Size: 1182 2158 6369 Fragments arranged by size: 6369 2158 1182 PfoI T'CCnGG_A Cuts at: 3581 4782 6940 3581 Size: 1201 2158 6350 Fragments arranged by size: 6350 2158 1201 PmeI GTTT'AAAC Cuts at: 7013 7013 Size: 9709 PmlI CAC'GTG Cuts at: 5521 5521 Size: 9709 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4716 4748 7778 7810 9291 9323 4716 Size: 32 3030 32 1481 32 5102 Fragments arranged by size: 5102 3030 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9709 PsiI TTA'TAA Cuts at: 7279 7279 Size: 9709 PstI C_TGCA'G Cuts at: 830 2722 7061 830 Size: 1892 4339 3478 Fragments arranged by size: 4339 3478 1892 PvuI CG_AT'CG Cuts at: 2001 4207 2001 Size: 2206 7503 PvuII CAG'CTG Cuts at: 3789 3789 Size: 9709 SacI G_AGCT'C Cuts at: 3329 4861 3329 Size: 1532 8177 SalI G'TCGA_C Cuts at: 1936 7051 1936 Size: 5115 4594 SapI GCTCTTCn'nnn_ Cuts at: 3334 3334 Size: 9709 SbfI CC_TGCA'GG Cuts at: 7061 7061 Size: 9709 ScaI AGT'ACT Cuts at: 5685 5791 7957 9669 5685 Size: 106 2166 1712 5725 Fragments arranged by size: 5725 2166 1712 106 SfcI C'TryA_G Cuts at: 826 1045 2247 2718 3523 4480 4752 5526 7057 Size: 219 1202 471 805 957 272 774 1531 Cuts at: 7057 8192 8873 9064 826 Size: 1135 681 191 1471 Fragments arranged by size: 1531 1471 1202 1135 957 805 774 681 471 272 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9709 SgrAI Cr'CCGG_yG Cuts at: 1700 4524 1700 Size: 2824 6885 SmaI CCC'GGG Cuts at: 1872 7036 1872 Size: 5164 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 3360 3439 3736 3965 4594 Size: 197 926 1349 68 79 297 229 629 Cuts at: 4594 4614 4733 4792 5698 7813 8714 8976 820 Size: 20 119 59 906 2115 901 262 1553 Fragments arranged by size: 2115 1553 1349 926 906 901 629 297 262 229 197 119 79 68 59 20 SnaBI TAC'GTA Cuts at: 493 7500 493 Size: 7007 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9709 SphI G_CATG'C Cuts at: 1132 7067 1132 Size: 5935 3774 SspI AAT'ATT Cuts at: 5 52 7633 5 Size: 47 7581 2081 Fragments arranged by size: 7581 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9709 StyI C'CwwG_G Cuts at: 513 1065 2945 6237 7148 513 Size: 552 1880 3292 911 3074 Fragments arranged by size: 3292 3074 1880 911 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3417 3489 3693 4209 5991 7736 7753 Size: 1255 1837 72 204 516 1782 1745 17 Cuts at: 7753 7906 8091 8536 325 Size: 153 185 445 1498 Fragments arranged by size: 1837 1782 1745 1498 1255 516 445 204 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2111 5683 5789 Size: 274 80 33 51 1443 134 3572 106 Cuts at: 5789 7955 9667 96 Size: 2166 1712 138 Fragments arranged by size: 3572 2166 1712 1443 274 138 134 106 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2990 3022 6853 6885 9669 9701 2990 Size: 32 3831 32 2784 32 2998 Fragments arranged by size: 3831 2998 2784 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9709 XbaI T'CTAG_A Cuts at: 2990 3332 5161 6208 7045 2990 Size: 342 1829 1047 837 5654 Fragments arranged by size: 5654 1829 1047 837 342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5870 5870 Size: 9709 XhoI C'TCGA_G Cuts at: 1943 3360 1943 Size: 1417 8292 XmaI C'CCGG_G Cuts at: 1870 7034 1870 Size: 5164 4545 XmnI GAAnn'nnTTC Cuts at: 3067 5791 5836 6891 7838 3067 Size: 2724 45 1055 947 4938 Fragments arranged by size: 4938 2724 1055 947 45 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7517 276 Size: 53 83 186 936 5983 2468 Fragments arranged by size: 5983 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI