Restriction map for
pFN21AA0853
(Linear) (Six-base) MAPSORT of: KIAA0853.seq Check: 8579 from: 1 to: 5004
KIAA0853.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4411 5004
Size: 4411 593
AccI GT'mk_AC
Cuts at: 0 946 1994 4874 5004
Size: 946 1048 2880 130
Fragments arranged by size:
2880 1048 946 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 668 2849 2978 3776 4773 4973 5004
Size: 157 511 2181 129 798 997 200 31
Fragments arranged by size:
2181 997 798 511 200 157 129 31
AfeI AGC'GCT
Cuts at: 0 2473 5004
Size: 2473 2531
AflIII A'CryG_T
Cuts at: 0 166 4642 5004
Size: 166 4476 362
Fragments arranged by size:
4476 362 166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2685 2717 5004
Size: 2685 32 2287
Fragments arranged by size:
2685 2287 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3197 4615 4992 5004
Size: 3197 1418 377 12
Fragments arranged by size:
3197 1418 377 12
ApaLI G'TGCA_C
Cuts at: 0 2798 5004
Size: 2798 2206
ApoI r'AATT_y
Cuts at: 0 747 2882 4412 4558 5004
Size: 747 2135 1530 146 446
Fragments arranged by size:
2135 1530 747 446 146
AseI AT'TA_AT
Cuts at: 0 467 529 5004
Size: 467 62 4475
Fragments arranged by size:
4475 467 62
AvaI C'yCGr_G
Cuts at: 0 1355 3574 4217 5004
Size: 1355 2219 643 787
Fragments arranged by size:
2219 1355 787 643
BanI G'GyrC_C
Cuts at: 0 4837 5004
Size: 4837 167
BanII G_rGCy'C
Cuts at: 0 299 1324 2856 4707 5004
Size: 299 1025 1532 1851 297
Fragments arranged by size:
1851 1532 1025 299 297
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 145 380 674 712 1878 2378 2654
Size: 72 73 235 294 38 1166 500 276
Cuts at: 2654 3467 3607 4741 4891 4924 5004
Size: 813 140 1134 150 33 80
Fragments arranged by size:
1166 1134 813 500 294 276 235 150
140 80 73 72 38 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3555 3589 5004
Size: 3555 34 1415
Fragments arranged by size:
3555 1415 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1551 4372 4990 5004
Size: 1551 2821 618 14
Fragments arranged by size:
2821 1551 618 14
BclI T'GATC_A
Cuts at: 0 1203 3753 5004
Size: 1203 2550 1251
Fragments arranged by size:
2550 1251 1203
BglII A'GATC_T
Cuts at: 0 3865 4257 5004
Size: 3865 392 747
Fragments arranged by size:
3865 747 392
Bme1580I G_kGCm'C
Cuts at: 0 2802 4840 5004
Size: 2802 2038 164
Fragments arranged by size:
2802 2038 164
BmgBI CAC'GTC
Cuts at: 0 285 5004
Size: 285 4719
BmrI ACTGGGnnnn_n'
Cuts at: 0 65 3770 5004
Size: 65 3705 1234
Fragments arranged by size:
3705 1234 65
BmtI G_CTAG'C
Cuts at: 0 692 5004
Size: 692 4312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 5004
Size: 342 4662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1401 3821 4391 4589 4919 5004
Size: 386 1015 2420 570 198 330 85
Fragments arranged by size:
2420 1015 570 386 330 198 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1272 1419 1716 1981 2594 2610 2713 2772
Size: 1272 147 297 265 613 16 103 59
Cuts at: 2772 3678 5004
Size: 906 1326
Fragments arranged by size:
1326 1272 906 613 297 265 147 103
59 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 1325 1880 2180 2186 5004
Size: 1325 555 300 6 2818
Fragments arranged by size:
2818 1325 555 300 6
BsaAI yAC'GTr
Cuts at: 0 784 3516 5004
Size: 784 2732 1488
Fragments arranged by size:
2732 1488 784
BsaBI GATnn'nnATC
Cuts at: 0 1544 1562 1820 2432 4256 5004
Size: 1544 18 258 612 1824 748
Fragments arranged by size:
1824 1544 748 612 258 18
BsaHI Gr'CG_yC
Cuts at: 0 1236 3508 5004
Size: 1236 2272 1496
Fragments arranged by size:
2272 1496 1236
BsaWI w'CCGG_w
Cuts at: 0 2535 5004
Size: 2535 2469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1074 1104 3267 3297 3579 3609 4848 4878
Size: 1074 30 2163 30 282 30 1239 30
Cuts at: 4878 5004
Size: 126
Fragments arranged by size:
2163 1239 1074 282 126 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 1034 2686 3275 3296 3576 3957 4471
Size: 331 703 1652 589 21 280 381 514
Cuts at: 4471 5004
Size: 533
Fragments arranged by size:
1652 703 589 533 514 381 331 280
21
BseYI C'CCAG_C
Cuts at: 0 94 954 1784 2494 3065 5004
Size: 94 860 830 710 571 1939
Fragments arranged by size:
1939 860 830 710 571 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3667 4291 4532 5004
Size: 3667 624 241 472
Fragments arranged by size:
3667 624 472 241
BsiEI CG_ry'CG
Cuts at: 0 2202 5004
Size: 2202 2802
BsiHKAI G_wGCw'C
Cuts at: 0 1324 2802 2856 4866 5004
Size: 1324 1478 54 2010 138
Fragments arranged by size:
2010 1478 1324 138 54
BsiWI C'GTAC_G
Cuts at: 0 1618 5004
Size: 1618 3386
BsmI GAATG_Cn'
Cuts at: 0 2798 3187 5004
Size: 2798 389 1817
Fragments arranged by size:
2798 1817 389
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1145 3499 5004
Size: 1145 2354 1505
Fragments arranged by size:
2354 1505 1145
Bsp1286I G_dGCh'C
Cuts at: 0 299 1324 2802 2856 4707 4840 4866 5004
Size: 299 1025 1478 54 1851 133 26 138
Fragments arranged by size:
1851 1478 1025 299 138 133 54 26
BspEI T'CCGG_A
Cuts at: 0 2535 5004
Size: 2535 2469
BspHI T'CATG_A
Cuts at: 0 483 1206 1227 1776 2856 5004
Size: 483 723 21 549 1080 2148
Fragments arranged by size:
2148 1080 723 549 483 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4411 4600 5004
Size: 4411 189 404
Fragments arranged by size:
4411 404 189
BsrBI CCG'CTC
Cuts at: 0 2064 2208 5004
Size: 2064 144 2796
Fragments arranged by size:
2796 2064 144
BsrDI GCAATG_nn'
Cuts at: 0 3144 5004
Size: 3144 1860
BsrFI r'CCGG_y
Cuts at: 0 2519 5004
Size: 2519 2485
BssSI C'ACGA_G
Cuts at: 0 194 1502 3488 4532 4857 5004
Size: 194 1308 1986 1044 325 147
Fragments arranged by size:
1986 1308 1044 325 194 147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3355 4426 5004
Size: 3355 1071 578
Fragments arranged by size:
3355 1071 578
BstBI TT'CG_AA
Cuts at: 0 4809 5004
Size: 4809 195
BstEII G'GTnAC_C
Cuts at: 0 227 5004
Size: 227 4777
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3649 5004
Size: 3649 1355
BstYI r'GATC_y
Cuts at: 0 249 2417 3865 4200 4257 4264 4470 5004
Size: 249 2168 1448 335 57 7 206 534
Fragments arranged by size:
2168 1448 534 335 249 206 57 7
BtgI C'CryG_G
Cuts at: 0 3091 5004
Size: 3091 1913
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1156 3523 5004
Size: 1156 2367 1481
Fragments arranged by size:
2367 1481 1156
BtsI GCAGTG_nn'
Cuts at: 0 3329 5004
Size: 3329 1675
ClaI AT'CG_AT
Cuts at: 0 3909 5004
Size: 3909 1095
DraIII CAC_nnn'GTG
Cuts at: 0 196 5004
Size: 196 4808
EaeI y'GGCC_r
Cuts at: 0 1587 3991 5004
Size: 1587 2404 1013
Fragments arranged by size:
2404 1587 1013
EarI CTCTTCn'nnn_
Cuts at: 0 553 1329 1971 1983 2255 2408 3302 5004
Size: 553 776 642 12 272 153 894 1702
Fragments arranged by size:
1702 894 776 642 553 272 153 12
EcoICRI GAG'CTC
Cuts at: 0 1322 2854 5004
Size: 1322 1532 2150
Fragments arranged by size:
2150 1532 1322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 386 668 1401 2849 2978 3776 3821
Size: 157 229 282 733 1448 129 798 45
Cuts at: 3821 4391 4589 4773 4919 4973 5004
Size: 570 198 184 146 54 31
Fragments arranged by size:
1448 798 733 570 282 229 198 184
157 146 129 54 45 31
EcoRV GAT'ATC
Cuts at: 0 819 1822 5004
Size: 819 1003 3182
Fragments arranged by size:
3182 1003 819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 965 1079 1874 3806 5004
Size: 965 114 795 1932 1198
Fragments arranged by size:
1932 1198 965 795 114
HaeII r_GCGC'y
Cuts at: 0 279 2475 2520 2712 5004
Size: 279 2196 45 192 2292
Fragments arranged by size:
2292 2196 279 192 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 307 339 1074 1106 1188 1220
Size: 43 32 232 32 735 32 82 32
Cuts at: 1220 1306 1338 1464 1480 1496 1512 2402 2434
Size: 86 32 126 16 16 16 890 32
Cuts at: 2434 2832 2864 2938 2970 3727 3759 4249 4281
Size: 398 32 74 32 757 32 490 32
Cuts at: 4281 4315 4347 4455 4487 5004
Size: 34 32 108 32 517
Fragments arranged by size:
890 757 735 517 490 398 232 126
108 86 82 74 43 34 32 32
32 32 32 32 32 32 32 32
32 32 16 16 16
HindIII A'AGCT_T
Cuts at: 0 4850 5004
Size: 4850 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 2904 5004
Size: 713 2191 2100
Fragments arranged by size:
2191 2100 713
MscI TGG'CCA
Cuts at: 0 3993 5004
Size: 3993 1011
MslI CAynn'nnrTG
Cuts at: 0 1205 1340 3647 5004
Size: 1205 135 2307 1357
Fragments arranged by size:
2307 1357 1205 135
MspA1I CmG'CkG
Cuts at: 0 1080 1784 5004
Size: 1080 704 3220
Fragments arranged by size:
3220 1080 704
NaeI GCC'GGC
Cuts at: 0 2521 5004
Size: 2521 2483
NgoMIV G'CCGG_C
Cuts at: 0 2519 5004
Size: 2519 2485
NheI G'CTAG_C
Cuts at: 0 688 5004
Size: 688 4316
NspI r_CATG'y
Cuts at: 0 170 1428 4646 5004
Size: 170 1258 3218 358
Fragments arranged by size:
3218 1258 358 170
PciI A'CATG_T
Cuts at: 0 166 4642 5004
Size: 166 4476 362
Fragments arranged by size:
4476 362 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 2443 5004
Size: 285 2158 2561
Fragments arranged by size:
2561 2158 285
PfoI T'CCnGG_A
Cuts at: 0 1576 2777 4935 5004
Size: 1576 1201 2158 69
Fragments arranged by size:
2158 1576 1201 69
PmlI CAC'GTG
Cuts at: 0 3516 5004
Size: 3516 1488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2711 2743 5004
Size: 2711 32 2261
Fragments arranged by size:
2711 2261 32
PstI C_TGCA'G
Cuts at: 0 717 5004
Size: 717 4287
PvuI CG_AT'CG
Cuts at: 0 2202 5004
Size: 2202 2802
PvuII CAG'CTG
Cuts at: 0 1784 5004
Size: 1784 3220
SacI G_AGCT'C
Cuts at: 0 1324 2856 5004
Size: 1324 1532 2148
Fragments arranged by size:
2148 1532 1324
SapI GCTCTTCn'nnn_
Cuts at: 0 1329 5004
Size: 1329 3675
ScaI AGT'ACT
Cuts at: 0 3680 3786 5004
Size: 3680 106 1218
Fragments arranged by size:
3680 1218 106
SfcI C'TryA_G
Cuts at: 0 242 713 1518 2475 2747 3521 5004
Size: 242 471 805 957 272 774 1483
Fragments arranged by size:
1483 957 805 774 471 272 242
SgrAI Cr'CCGG_yG
Cuts at: 0 2519 5004
Size: 2519 2485
SmlI C'TyrA_G
Cuts at: 0 1287 1355 1434 1731 1960 2589 2609 2728
Size: 1287 68 79 297 229 629 20 119
Cuts at: 2728 2787 3693 5004
Size: 59 906 1311
Fragments arranged by size:
1311 1287 906 629 297 229 119 79
68 59 20
StyI C'CwwG_G
Cuts at: 0 940 4232 5004
Size: 940 3292 772
Fragments arranged by size:
3292 940 772
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1412 1484 1688 2204 3986 5004
Size: 1412 72 204 516 1782 1018
Fragments arranged by size:
1782 1412 1018 516 204 72
TatI w'GTAC_w
Cuts at: 0 106 3678 3784 5004
Size: 106 3572 106 1220
Fragments arranged by size:
3572 1220 106 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 985 1017 4848 4880 5004
Size: 985 32 3831 32 124
Fragments arranged by size:
3831 985 124 32 32
XbaI T'CTAG_A
Cuts at: 0 985 1327 3156 4203 5004
Size: 985 342 1829 1047 801
Fragments arranged by size:
1829 1047 985 801 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3865 5004
Size: 3865 1139
XhoI C'TCGA_G
Cuts at: 0 1355 5004
Size: 1355 3649
XmnI GAAnn'nnTTC
Cuts at: 0 1062 3786 3831 4886 5004
Size: 1062 2724 45 1055 118
Fragments arranged by size:
2724 1062 1055 118 45
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AloI AlwNI ApaLI
ApoI AseI AvaI BanI BanII BbsI BcgI BciVI
BclI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
DraIII EaeI EarI EcoICRI Eco57MI EcoRV FalI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV
NheI NspI PciI PflMI PfoI PmlI PpiI PstI
PvuI PvuII SacI SapI ScaI SfcI SgrAI SmlI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
ApaI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BfrBI BglI BlpI Bpu10I BsrGI BssHII BstZ17I Bsu36I
CspCI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NotI NruI NsiI PacI
PasI PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XmaI ZraI