(Circular) (Six-base) MAPSORT of: pFN21AA0834.seq Check: 857 from: 1 to: 6058 pFN21AA0834 6058 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2891 2891 Size: 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2093 2397 3401 1937 Size: 156 304 1004 4594 Fragments arranged by size: 4594 1004 304 156 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2459 4121 5505 5773 1632 Size: 827 1662 1384 268 1917 Fragments arranged by size: 1917 1662 1384 827 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 2892 3243 4957 5934 1203 Size: 1689 351 1714 977 1327 Fragments arranged by size: 1714 1689 1327 977 351 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AlwNI CAG_nnn'CTG Cuts at: 2671 3290 3801 5373 2671 Size: 619 511 1572 3356 Fragments arranged by size: 3356 1572 619 511 ApaLI G'TGCA_C Cuts at: 2588 4115 5271 2588 Size: 1527 1156 3375 Fragments arranged by size: 3375 1527 1156 ApoI r'AATT_y Cuts at: 1493 2333 3367 3593 3745 5854 1493 Size: 840 1034 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 840 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 3383 1870 Size: 73 1440 4545 Fragments arranged by size: 4545 1440 73 BamHI G'GATC_C Cuts at: 3388 3755 3388 Size: 367 5691 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2043 3377 1391 Size: 652 1334 4072 Fragments arranged by size: 4072 1334 652 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3240 928 Size: 971 1341 3746 Fragments arranged by size: 3746 1341 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4249 4283 1903 Size: 34 50 34 2228 34 3678 Fragments arranged by size: 3678 2228 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3950 5160 3950 Size: 1210 4848 BclI T'GATC_A Cuts at: 1548 2124 2445 2739 1548 Size: 576 321 294 4867 Fragments arranged by size: 4867 576 321 294 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3223 4669 136 Size: 107 122 71 631 605 1551 1446 1525 Fragments arranged by size: 1551 1525 1446 631 605 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 3107 3475 3107 Size: 368 5690 Bme1580I G_kGCm'C Cuts at: 1790 2592 4119 5275 1790 Size: 802 1527 1156 2573 Fragments arranged by size: 2573 1527 1156 802 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3789 4747 442 Size: 416 53 83 399 24 2372 958 1753 Fragments arranged by size: 2372 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2832 3045 3312 4718 1239 Size: 1051 542 213 267 1406 2579 Fragments arranged by size: 2579 1406 1051 542 267 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3211 4183 5048 5346 3211 Size: 972 865 298 3923 Fragments arranged by size: 3923 972 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2709 4721 882 Size: 617 1210 2012 2219 Fragments arranged by size: 2219 2012 1210 617 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 6052 6052 Size: 6058 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4247 4882 275 Size: 53 83 186 936 2332 382 635 1451 Fragments arranged by size: 2332 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2798 2828 3122 3152 1178 Size: 30 165 30 1395 30 294 30 4084 Fragments arranged by size: 4084 1395 294 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2257 1186 Size: 1071 4987 BseYI C'CCAG_C Cuts at: 2312 5261 5757 2312 Size: 2949 496 2613 Fragments arranged by size: 2949 2613 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2592 4119 4204 5275 2186 Size: 406 1527 85 1071 2969 Fragments arranged by size: 2969 1527 1071 406 85 BsmI GAATG_Cn' Cuts at: 2230 3569 3662 2230 Size: 1339 93 4626 Fragments arranged by size: 4626 1339 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2555 2555 Size: 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2186 2592 3377 4119 4204 5275 Size: 399 253 143 406 785 742 85 1071 Cuts at: 5275 1391 Size: 2174 Fragments arranged by size: 2174 1071 785 742 406 399 253 143 85 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 2539 3947 5677 2539 Size: 1408 1730 2920 Fragments arranged by size: 2920 1730 1408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 3222 3413 844 Size: 2047 331 191 3489 Fragments arranged by size: 3489 2047 331 191 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2032 4553 4727 65 Size: 1183 784 2521 174 1396 Fragments arranged by size: 2521 1396 1183 784 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3069 96 Size: 2973 3085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 3197 4118 5130 3197 Size: 921 1012 4125 Fragments arranged by size: 4125 1012 921 BstAPI GCAn_nnn'nTGC Cuts at: 794 3185 794 Size: 2391 3667 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 2827 1184 Size: 1643 4415 BstXI CCAn_nnnn'nTGG Cuts at: 2824 3228 2824 Size: 404 5654 BstYI r'GATC_y Cuts at: 1972 2381 3388 3755 4140 4157 5609 5695 5707 Size: 409 1007 367 385 17 1452 86 12 Cuts at: 5707 6053 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1007 409 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2398 2398 Size: 6058 Bsu36I CC'TnA_GG Cuts at: 2148 2148 Size: 6058 BtgI C'CryG_G Cuts at: 513 1065 3221 513 Size: 552 2156 3350 Fragments arranged by size: 3350 2156 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3574 4368 4388 3574 Size: 794 20 5244 Fragments arranged by size: 5244 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2869 2904 529 Size: 35 2305 35 3683 Fragments arranged by size: 3683 2305 35 35 DraI TTT'AAA Cuts at: 2505 3362 2505 Size: 857 5201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3505 4394 6023 Size: 54 1425 50 275 18 1675 889 1629 Cuts at: 6023 8 Size: 43 Fragments arranged by size: 1675 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3988 1353 Size: 2635 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2459 2832 3045 3312 4121 4718 Size: 393 658 169 373 213 267 809 597 Cuts at: 4718 5505 5773 1239 Size: 787 268 1524 Fragments arranged by size: 1524 809 787 658 597 393 373 268 267 213 169 EcoO109I rG'GnC_Cy Cuts at: 3130 3130 Size: 6058 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2516 2516 Size: 6058 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 5205 1678 Size: 3527 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3122 3154 4699 4731 4773 Size: 32 135 32 1550 32 1545 32 42 Cuts at: 4773 4805 1373 Size: 32 2626 Fragments arranged by size: 2626 1550 1545 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 2493 748 Size: 1745 4313 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 3657 3657 Size: 6058 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2841 5172 5356 1305 Size: 1536 2331 184 2007 Fragments arranged by size: 2331 2007 1536 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4236 Fragments arranged by size: 4236 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2074 2087 3226 3242 4016 4375 4534 Size: 648 908 13 1139 16 774 359 159 Cuts at: 4534 518 Size: 2042 Fragments arranged by size: 2042 1139 908 774 648 359 159 16 13 MspA1I CmG'CkG Cuts at: 1606 3474 4151 5299 5544 1606 Size: 1868 677 1148 245 2120 Fragments arranged by size: 2120 1868 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5506 NdeI CA'TA_TG Cuts at: 387 2353 387 Size: 1966 4092 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 2522 2896 3247 3304 3416 4961 1132 Size: 97 1293 374 351 57 112 1545 2229 Fragments arranged by size: 2229 1545 1293 374 351 112 97 57 PasI CC'CwG_GG Cuts at: 1348 2624 1348 Size: 1276 4782 PciI A'CATG_T Cuts at: 2892 3243 4957 2892 Size: 351 1714 3993 Fragments arranged by size: 3993 1714 351 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 3290 1108 Size: 1005 1177 3876 Fragments arranged by size: 3876 1177 1005 PfoI T'CCnGG_A Cuts at: 2947 2947 Size: 6058 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4127 4159 5640 5672 4127 Size: 32 1481 32 4513 Fragments arranged by size: 4513 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3130 3130 Size: 6058 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 3628 3628 Size: 6058 PstI C_TGCA'G Cuts at: 830 2038 2457 3410 830 Size: 1208 419 953 3478 Fragments arranged by size: 3478 1208 953 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2034 2391 2453 3406 4541 5222 5413 Size: 219 989 357 62 953 1135 681 191 Cuts at: 5413 826 Size: 1471 Fragments arranged by size: 1471 1135 989 953 681 357 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 3385 1872 Size: 1513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4162 5063 5325 820 Size: 197 926 1247 972 901 262 1553 Fragments arranged by size: 1553 1247 972 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3304 3416 1132 Size: 2172 112 3774 Fragments arranged by size: 3774 2172 112 SspI AAT'ATT Cuts at: 5 52 2876 2985 3982 5 Size: 47 2824 109 997 2081 Fragments arranged by size: 2824 2081 997 109 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2556 2929 3497 513 Size: 552 1491 373 568 3074 Fragments arranged by size: 3074 1491 568 552 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2765 4085 4102 4255 4440 4885 325 Size: 1255 1185 1320 17 153 185 445 1498 Fragments arranged by size: 1498 1320 1255 1185 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 3069 4304 Size: 274 80 33 51 1443 630 462 1235 Cuts at: 4304 6016 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1235 630 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 6018 6050 2798 Size: 32 3188 32 2806 Fragments arranged by size: 3188 2806 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 3383 1870 Size: 1513 4545 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI