(Circular) (Six-base) MAPSORT of: pFN21AA0820.seq Check: 8276 from: 1 to: 7282 pFN21AA0820 7282 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2841 2841 Size: 7282 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5092 278 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3780 3831 3972 4625 1937 Size: 1843 51 141 653 4594 Fragments arranged by size: 4594 1843 653 141 51 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 4050 5409 5782 1563 Size: 2487 1359 373 3063 Fragments arranged by size: 3063 2487 1359 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2875 4080 5345 6729 6997 1632 Size: 953 290 1205 1265 1384 268 1917 Fragments arranged by size: 1917 1384 1265 1205 953 290 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 2058 2386 2388 3981 6181 7158 1203 Size: 855 328 2 1593 2200 977 1327 Fragments arranged by size: 2200 1593 1327 977 855 328 2 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 3396 1166 Size: 2230 5052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3765 1184 Size: 2581 4701 AlwNI CAG_nnn'CTG Cuts at: 3360 4364 5025 6597 3360 Size: 1004 661 1572 4045 Fragments arranged by size: 4045 1572 1004 661 ApaI G_GGCC'C Cuts at: 4498 4498 Size: 7282 ApaLI G'TGCA_C Cuts at: 5339 6495 5339 Size: 1156 6126 ApoI r'AATT_y Cuts at: 1493 2273 2950 4066 4591 4817 4969 7078 1493 Size: 780 677 1116 525 226 152 2109 1697 Fragments arranged by size: 2109 1697 1116 780 677 525 226 152 AseI AT'TA_AT Cuts at: 160 1032 2364 3308 5836 160 Size: 872 1332 944 2528 1606 Fragments arranged by size: 2528 1606 1332 944 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2178 4607 1870 Size: 73 204 31 2429 4545 Fragments arranged by size: 4545 2429 204 73 31 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3985 4018 3985 Size: 33 7249 BamHI G'GATC_C Cuts at: 4612 4979 4612 Size: 367 6915 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4234 4603 6058 618 Size: 325 217 627 2447 369 1455 1842 Fragments arranged by size: 2447 1842 1455 627 369 325 217 BanII G_rGCy'C Cuts at: 1391 2141 4077 4399 4465 4498 4601 1391 Size: 750 1936 322 66 33 103 4072 Fragments arranged by size: 4072 1936 750 322 103 66 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 2984 3419 3483 928 Size: 971 1071 14 435 64 4727 Fragments arranged by size: 4727 1071 971 435 64 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5473 5507 1903 Size: 34 3536 34 3678 Fragments arranged by size: 3678 3536 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2869 3605 4013 4254 5174 6384 2869 Size: 736 408 241 920 1210 3767 Fragments arranged by size: 3767 1210 920 736 408 241 BclI T'GATC_A Cuts at: 1548 4041 1548 Size: 2493 4789 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2762 5893 136 Size: 107 122 71 631 605 1090 3131 1525 Fragments arranged by size: 3131 1525 1090 631 605 122 107 71 BglII A'GATC_T Cuts at: 4009 7277 4009 Size: 3268 4014 BlpI GC'TnA_GC Cuts at: 3501 4131 4140 4699 3501 Size: 630 9 559 6084 Fragments arranged by size: 6084 630 559 9 Bme1580I G_kGCm'C Cuts at: 1790 4498 5343 6499 1790 Size: 2708 845 1156 2573 Fragments arranged by size: 2708 2573 1156 845 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4497 5013 5971 Size: 416 53 83 399 24 3080 516 958 Cuts at: 5971 442 Size: 1753 Fragments arranged by size: 3080 1753 958 516 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2573 1056 Size: 1517 5765 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2514 3201 4227 4386 5942 1239 Size: 872 403 687 1026 159 1556 2579 Fragments arranged by size: 2579 1556 1026 872 687 403 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 2574 3821 5407 6272 6570 2321 Size: 253 1247 1586 865 298 3033 Fragments arranged by size: 3033 1586 1247 865 298 253 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3947 4513 5945 882 Size: 617 2448 566 1432 2219 Fragments arranged by size: 2448 2219 1432 617 566 BsaAI yAC'GTr Cuts at: 493 1204 2389 2698 5073 493 Size: 711 1185 309 2375 2702 Fragments arranged by size: 2702 2375 1185 711 309 BsaBI GATnn'nnATC Cuts at: 7276 7276 Size: 7282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5089 5471 6106 275 Size: 53 83 186 936 3556 382 635 1451 Fragments arranged by size: 3556 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5715 6113 6387 6534 6945 5715 Size: 398 274 147 411 6052 Fragments arranged by size: 6052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7057 Fragments arranged by size: 7057 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 3523 4275 4389 1186 Size: 853 1484 752 114 4079 Fragments arranged by size: 4079 1484 853 752 114 BseYI C'CCAG_C Cuts at: 2071 3860 4439 6485 6981 2071 Size: 1789 579 2046 496 2372 Fragments arranged by size: 2372 2046 1789 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6709 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4351 4732 5493 6521 1540 Size: 275 186 2350 381 761 1028 2301 Fragments arranged by size: 2350 2301 1028 761 381 275 186 BsiHKAI G_wGCw'C Cuts at: 2141 2148 4077 4327 5343 5428 6499 2141 Size: 7 1929 250 1016 85 1071 2924 Fragments arranged by size: 2924 1929 1071 1016 250 85 7 BsmI GAATG_Cn' Cuts at: 4793 4886 4793 Size: 93 7189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2148 4077 4327 4399 4465 4498 Size: 399 351 7 1929 250 72 66 33 Cuts at: 4498 4601 5343 5428 6499 1391 Size: 103 742 85 1071 2174 Fragments arranged by size: 2174 1929 1071 742 399 351 250 103 85 72 66 33 7 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 3103 3591 5171 6901 3103 Size: 488 1580 1730 3484 Fragments arranged by size: 3484 1730 1580 488 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2841 4266 4637 844 Size: 1997 1425 371 3489 Fragments arranged by size: 3489 1997 1425 371 BsrBI CCG'CTC Cuts at: 1394 1823 5169 1394 Size: 429 3346 3507 Fragments arranged by size: 3507 3346 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2257 2987 5777 5951 65 Size: 1183 1009 730 2790 174 1396 Fragments arranged by size: 2790 1396 1183 1009 730 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 5926 6113 1700 Size: 110 319 3797 187 2869 Fragments arranged by size: 3797 2869 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7282 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 2497 5342 6354 2497 Size: 2845 1012 3425 Fragments arranged by size: 3425 2845 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 794 Size: 1449 5833 BstBI TT'CG_AA Cuts at: 4162 6086 4162 Size: 1924 5358 BstEII G'GTnAC_C Cuts at: 1184 4278 1184 Size: 3094 4188 BstXI CCAn_nnnn'nTGG Cuts at: 3340 3340 Size: 7282 BstYI r'GATC_y Cuts at: 1972 3289 4009 4612 4979 5364 5381 6833 6919 Size: 1317 720 603 367 385 17 1452 86 Cuts at: 6919 6931 7277 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1317 720 603 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3781 3781 Size: 7282 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 3924 513 Size: 552 939 105 1815 3871 Fragments arranged by size: 3871 1815 939 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6543 Fragments arranged by size: 6543 683 56 BtsI GCAGTG_nn' Cuts at: 4453 4798 5592 5612 4453 Size: 345 794 20 6123 Fragments arranged by size: 6123 794 345 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7247 DraI TTT'AAA Cuts at: 4586 4586 Size: 7282 DraIII CAC_nnn'GTG Cuts at: 4322 4322 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3449 6289 809 Size: 507 2133 2840 1802 Fragments arranged by size: 2840 2133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 4215 4348 Size: 54 1425 50 275 18 289 2096 133 Cuts at: 4348 4729 5618 7247 8 Size: 381 889 1629 43 Fragments arranged by size: 2096 1629 1425 889 381 289 275 133 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4348 4729 1537 Size: 275 2536 381 4090 Fragments arranged by size: 4090 2536 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 3023 3154 3275 5212 1353 Size: 1335 335 131 121 1937 3423 Fragments arranged by size: 3423 1937 1335 335 131 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4544 5869 6243 6389 387 Size: 1064 138 2955 1325 374 146 1280 Fragments arranged by size: 2955 1325 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2139 4075 2139 Size: 1936 5346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2514 2585 2875 3201 4080 4227 Size: 393 479 403 71 290 326 879 147 Cuts at: 4227 4386 5345 5942 6729 6997 1239 Size: 159 959 597 787 268 1524 Fragments arranged by size: 1524 959 879 787 597 479 403 393 326 290 268 159 147 71 EcoNI CCTnn'n_nnAGG Cuts at: 2837 4118 2837 Size: 1281 6001 EcoO109I rG'GnC_Cy Cuts at: 2842 3269 4494 4495 2842 Size: 427 1225 1 5629 Fragments arranged by size: 5629 1225 427 1 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 EcoRV GAT'ATC Cuts at: 2458 2458 Size: 7282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2315 3763 4205 2315 Size: 1448 442 5392 Fragments arranged by size: 5392 1448 442 FspI TGC'GCA Cuts at: 5788 5788 Size: 7282 HaeII r_GCGC'y Cuts at: 1678 2072 2130 4147 6429 1678 Size: 394 58 2017 2282 2531 Fragments arranged by size: 2531 2282 2017 394 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 3274 3306 3499 Size: 32 135 32 1329 32 341 32 193 Cuts at: 3499 3531 3849 3881 5923 5955 5997 6029 1373 Size: 32 318 32 2042 32 42 32 2626 Fragments arranged by size: 2626 2042 1329 341 318 193 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3832 4626 4872 669 Size: 988 281 1894 794 246 3079 Fragments arranged by size: 3079 1894 988 794 281 246 HindIII A'AGCT_T Cuts at: 748 3637 748 Size: 2889 4393 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 3935 4881 3935 Size: 946 6336 MluI A'CGCG_T Cuts at: 2058 7158 2058 Size: 5100 2182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3520 6396 6580 1305 Size: 2215 2876 184 2007 Fragments arranged by size: 2876 2215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5460 Fragments arranged by size: 5460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3396 5240 5599 5758 518 Size: 648 2230 1844 359 159 2042 Fragments arranged by size: 2230 2042 1844 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 3837 4698 5375 6523 6768 1606 Size: 432 1799 861 677 1148 245 2120 Fragments arranged by size: 2120 1799 1148 861 677 432 245 NaeI GCC'GGC Cuts at: 2131 2131 Size: 7282 NcoI C'CATG_G Cuts at: 513 1065 2004 3924 513 Size: 552 939 1920 3871 Fragments arranged by size: 3871 1920 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7282 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 7282 NheI G'CTAG_C Cuts at: 1052 2569 1052 Size: 1517 5765 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 3685 3813 3985 4640 6185 1132 Size: 97 2456 128 172 655 1545 2229 Fragments arranged by size: 2456 2229 1545 655 172 128 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7282 PciI A'CATG_T Cuts at: 3981 6181 3981 Size: 2200 5082 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7282 PfoI T'CCnGG_A Cuts at: 4256 4256 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PmlI CAC'GTG Cuts at: 2389 2389 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5351 5383 6864 6896 5351 Size: 32 1481 32 5737 Fragments arranged by size: 5737 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2842 2842 Size: 7282 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7282 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PspOMI G'GGCC_C Cuts at: 4494 4494 Size: 7282 PspXI vC'TCGA_Gb Cuts at: 2178 2178 Size: 7282 PstI C_TGCA'G Cuts at: 830 2055 2220 2838 2997 4634 830 Size: 1225 165 618 159 1637 3478 Fragments arranged by size: 3478 1637 1225 618 165 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 PvuII CAG'CTG Cuts at: 3837 3837 Size: 7282 SacI G_AGCT'C Cuts at: 2141 4077 2141 Size: 1936 5346 SalI G'TCGA_C Cuts at: 1936 3830 4624 1936 Size: 1894 794 4594 Fragments arranged by size: 4594 1894 794 SbfI CC_TGCA'GG Cuts at: 4634 4634 Size: 7282 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 3423 3423 Size: 7282 SfcI C'TryA_G Cuts at: 826 1045 2051 2216 2834 2903 2993 3882 4630 Size: 219 1006 165 618 69 90 889 748 Cuts at: 4630 5765 6446 6637 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 889 748 681 618 219 191 165 90 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7282 SmaI CCC'GGG Cuts at: 1872 4609 1872 Size: 2737 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2178 2300 2589 3800 5386 Size: 197 926 204 31 122 289 1211 1586 Cuts at: 5386 6287 6549 820 Size: 901 262 1553 Fragments arranged by size: 1586 1553 1211 926 901 289 262 204 197 122 31 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 4640 1132 Size: 3508 3774 SspI AAT'ATT Cuts at: 5 52 5206 5 Size: 47 5154 2081 Fragments arranged by size: 5154 2081 47 StuI AGG'CCT Cuts at: 1517 4486 1517 Size: 2969 4313 StyI C'CwwG_G Cuts at: 513 1065 2004 3690 3924 4189 4721 513 Size: 552 939 1686 234 265 532 3074 Fragments arranged by size: 3074 1686 939 552 532 265 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2829 5309 5326 5479 5664 6109 325 Size: 1255 1249 2480 17 153 185 445 1498 Fragments arranged by size: 2480 1498 1255 1249 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3678 5528 7240 Size: 274 80 33 51 1443 1701 1850 1712 Cuts at: 7240 96 Size: 138 Fragments arranged by size: 1850 1712 1701 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3534 3566 7242 7274 3534 Size: 32 3676 32 3542 Fragments arranged by size: 3676 3542 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7282 XbaI T'CTAG_A Cuts at: 2662 4618 2662 Size: 1956 5326 XhoI C'TCGA_G Cuts at: 1943 2147 2178 1943 Size: 204 31 7047 Fragments arranged by size: 7047 204 31 XmaI C'CCGG_G Cuts at: 1870 4607 1870 Size: 2737 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3111 5411 2777 Size: 334 2300 4648 Fragments arranged by size: 4648 2300 334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5090 276 Size: 53 83 186 936 3556 2468 Fragments arranged by size: 3556 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI