Restriction map for
pFN21AA0816
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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI BcgI BfrI BglII BsiWI
BspDI BspHI BstBI ClaI DraI
EagI EclXI Eco52I EcoNI EcoRI
FseI HindIII KspI MluI NheI
NotI NruI NspV PacI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SexAI SfiI SfuI SnaBI
SpeI SphI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AgeI 3750 ACCGGT A^CCGGT
AhdI 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5111 GTGCAC G^TGCAC
AocI 2672 CCTCAGG CC^T[ACGT]AGG
ApaI 1625 GGGCCC GGGCC^C
ApaLI 5111 GTGCAC G^TGCAC
AspEI 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1472 GGATCC G^GATCC
BciVI 2433 GGATAC GTATCC(6/5)
BclI 4394 TGATCA T^GATCA
BsiEI 1284 CGACCG CG[GA][CT]^CG
BssHII 17 GCGCGC G^CGCGC
BssSI 3862 CTCGTG CACGAG(-5/-1)
BstEII 2475 GGTCACC G^GT[ACGT]ACC
BstPI 2475 GGTCACC G^GT[ACGT]ACC
Bsu36I 2672 CCTCAGG CC^T[ACGT]AGG
CpoI 3580 CGGTCCG CG^G[AT]CCG
DraIII 3011 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2672 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2475 GGTCACC G^GT[ACGT]ACC
EcoRV 4795 GATATC GAT^ATC
EcoT22I 5514 ATGCAT ATGCA^T
FbaI 4394 TGATCA T^GATCA
HpaI 1078 GTTAAC GTT^AAC
MfeI 2777 CAATTG C^AATTG
MunI 2777 CAATTG C^AATTG
NdeI 3764 CATATG CA^TATG
NsiI 5514 ATGCAT ATGCA^T
PflFI 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3750 ACCGGT A^CCGGT
RsrII 3580 CGGTCCG CG^G[AT]CCG
SacI 4147 GAGCTC GAGCT^C
SbfI 2086 CCTGCAGG CCTGCA^GG
SgrAI 3749 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 2086 CCTGCAGG CCTGCA^GG
Tth111I 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccIII 368 TCCGGA T^CCGGA
3583 TCCGGA
AcsI 2002 AAATTT [GA]^AATT[CT]
2782 GAATTT
Aor51HI 1993 AGCGCT AGC^GCT
2908 AGCGCT
ApoI 2002 AAATTT [GA]^AATT[CT]
2782 GAATTT
AvrII 3627 CCTAGG C^CTAGG
4966 CCTAGG
BbeI 31 GGCGCC GGCGC^C
1826 GGCGCC
BbrPI 1173 CACGTG CAC^GTG
2356 CACGTG
BbvCI 548 CCTCAGC CCTCAGC(-5/-2)
4813 CCTCAGC
BlnI 3627 CCTAGG C^CTAGG
4966 CCTAGG
BlpI 484 GCTGAGC GC^T[ACGT]AGC
1849 GCTAAGC
Bpu1102I 484 GCTGAGC GC^T[ACGT]AGC
1849 GCTAAGC
BsaAI 1173 CACGTG [CT]AC^GT[GA]
2356 CACGTG
BsaI 4057 GAGACC GGTCTC(1/5)
3183 GGTCTC
BseAI 368 TCCGGA T^CCGGA
3583 TCCGGA
BsmBI 5054 CGTCTC CGTCTC(1/5)
5572 GAGACG
BsmI 1404 GAATGC GAATGC(1/-1)
3564 GAATGC
Bsp1407I 5228 TGTACA T^GTACA
5348 TGTACA
BspEI 368 TCCGGA T^CCGGA
3583 TCCGGA
BspLU11I 97 ACATGT A^CATGT
2615 ACATGT
BsrGI 5228 TGTACA T^GTACA
5348 TGTACA
Bst1107I 197 GTATAC GTA^TAC
3412 GTATAC
BstXI 624 CCACTGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2838 CCACCCATTTGG
BstZ17I 197 GTATAC GTA^TAC
3412 GTATAC
CelII 484 GCTGAGC GC^T[ACGT]AGC
1849 GCTAAGC
DrdI 2971 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5046 GACCCGCACGTC
Eco47III 1993 AGCGCT AGC^GCT
2908 AGCGCT
KasI 31 GGCGCC G^GCGCC
1826 GGCGCC
MroI 368 TCCGGA T^CCGGA
3583 TCCGGA
NarI 31 GGCGCC GG^CGCC
1826 GGCGCC
NcoI 1688 CCATGG C^CATGG
2603 CCATGG
PaeR7I 1596 CTCGAG C^TCGAG
3942 CTCGAG
PmaCI 1173 CACGTG CAC^GTG
2356 CACGTG
PmlI 1173 CACGTG CAC^GTG
2356 CACGTG
SapI 5593 GAAGAGC GCTCTTC(1/4)
4068 GCTCTTC
ScaI 2553 AGTACT AGT^ACT
4389 AGTACT
SfoI 31 GGCGCC GGC^GCC
1826 GGCGCC
SspBI 5228 TGTACA T^GTACA
5348 TGTACA
StuI 1739 AGGCCT AGG^CCT
3882 AGGCCT
XhoI 1596 CTCGAG C^TCGAG
3942 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
Asp700I Asp718I AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI