(Circular) (Six-base) MAPSORT of: pFN21AA0816.seq Check: 6181 from: 1 to: 10411 pFN21AA0816 10411 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3349 3349 Size: 10411 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8221 278 Size: 53 83 186 936 6685 2468 Fragments arranged by size: 6685 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2203 3524 5012 5268 5418 7754 1937 Size: 266 1321 1488 256 150 2336 4594 Fragments arranged by size: 4594 2336 1488 1321 266 256 150 Acc65I G'GTAC_C Cuts at: 3701 5224 6444 6958 7732 3701 Size: 1523 1220 514 774 6380 Fragments arranged by size: 6380 1523 1220 774 514 AclI AA'CG_TT Cuts at: 1563 8538 8911 1563 Size: 6975 373 3063 Fragments arranged by size: 6975 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 6047 6305 8474 9858 10126 1632 Size: 4415 258 2169 1384 268 1917 Fragments arranged by size: 4415 2169 1917 1384 268 258 AfeI AGC'GCT Cuts at: 4000 4915 4000 Size: 915 9496 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10214 AflIII A'CryG_T Cuts at: 1203 2102 3177 4362 4620 9310 10287 1203 Size: 899 1075 1185 258 4690 977 1327 Fragments arranged by size: 4690 1327 1185 1075 977 899 258 AgeI A'CCGG_T Cuts at: 5755 9242 5755 Size: 3487 6924 AhdI GACnn_n'nnGTC Cuts at: 7537 9140 7537 Size: 1603 8808 AleI CACnn'nnGTG Cuts at: 1166 4209 6131 6212 1166 Size: 3043 1922 81 5365 Fragments arranged by size: 5365 3043 1922 81 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5126 1184 Size: 3942 6469 AlwNI CAG_nnn'CTG Cuts at: 2956 3323 3364 3661 8154 9726 2956 Size: 367 41 297 4493 1572 3641 Fragments arranged by size: 4493 3641 1572 367 297 41 ApaI G_GGCC'C Cuts at: 3634 3634 Size: 10411 ApaLI G'TGCA_C Cuts at: 7116 8468 9624 7116 Size: 1352 1156 7903 Fragments arranged by size: 7903 1352 1156 ApoI r'AATT_y Cuts at: 1493 4007 4787 7720 7946 8098 10207 1493 Size: 2514 780 2933 226 152 2109 1697 Fragments arranged by size: 2933 2514 2109 1697 780 226 152 AseI AT'TA_AT Cuts at: 160 1032 8965 160 Size: 872 7933 1606 Fragments arranged by size: 7933 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10411 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2325 3601 3672 4350 4587 5511 Size: 73 129 253 1276 71 678 237 924 Cuts at: 5511 5947 7736 1870 Size: 436 1789 4545 Fragments arranged by size: 4545 1789 1276 924 678 436 253 237 129 73 71 AvrII C'CTAG_G Cuts at: 5632 6971 5632 Size: 1339 9072 BamHI G'GATC_C Cuts at: 3477 7741 8108 3477 Size: 4264 367 5780 Fragments arranged by size: 5780 4264 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 3596 3701 3831 5224 Size: 325 217 627 249 1560 105 130 1393 Cuts at: 5224 5729 6444 6958 7732 9187 618 Size: 505 715 514 774 1455 1842 Fragments arranged by size: 1842 1560 1455 1393 774 715 627 514 505 325 249 217 130 105 BanII G_rGCy'C Cuts at: 1391 3542 3634 4457 4649 5391 6156 7001 7730 Size: 2151 92 823 192 742 765 845 729 Cuts at: 7730 1391 Size: 4072 Fragments arranged by size: 4072 2151 845 823 765 742 729 192 92 BbeI G_GCGC'C Cuts at: 2040 3835 2040 Size: 1795 8616 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3442 3960 4784 4978 6047 6293 7532 Size: 971 1543 518 824 194 1069 246 1239 Cuts at: 7532 928 Size: 3807 Fragments arranged by size: 3807 1543 1239 1069 971 824 518 246 194 BbvCI CC'TCA_GC Cuts at: 2554 6819 2554 Size: 4265 6146 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8602 8636 1903 Size: 34 6665 34 3678 Fragments arranged by size: 6665 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4432 8303 9513 4432 Size: 3871 1210 5330 Fragments arranged by size: 5330 3871 1210 BclI T'GATC_A Cuts at: 1548 6399 1548 Size: 4851 5560 BfrBI ATG'CAT Cuts at: 7521 7521 Size: 10411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2361 3150 3292 Size: 107 122 71 631 605 689 789 142 Cuts at: 3292 3546 4182 4461 5100 6015 9022 136 Size: 254 636 279 639 915 3007 1525 Fragments arranged by size: 3007 1525 915 789 689 639 636 631 605 279 254 142 122 107 71 BglII A'GATC_T Cuts at: 10406 10406 Size: 10411 BlpI GC'TnA_GC Cuts at: 2490 3855 7828 2490 Size: 1365 3973 5073 Fragments arranged by size: 5073 3973 1365 Bme1580I G_kGCm'C Cuts at: 1790 3103 3177 3634 4216 5920 7120 8472 9628 Size: 1313 74 457 582 1704 1200 1352 1156 Cuts at: 9628 1790 Size: 2573 Fragments arranged by size: 2573 1704 1352 1313 1200 1156 582 457 74 BmgBI CAC'GTC Cuts at: 1318 4318 7059 1318 Size: 3000 2741 4670 Fragments arranged by size: 4670 3000 2741 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 3537 3730 Size: 416 53 83 399 24 723 1397 193 Cuts at: 3730 3853 4143 4351 4645 4768 5000 5569 6322 Size: 123 290 208 294 123 232 569 753 Cuts at: 6322 6472 6595 6995 8142 9100 442 Size: 150 123 400 1147 958 1753 Fragments arranged by size: 1753 1397 1147 958 753 723 569 416 400 399 294 290 232 208 193 150 123 123 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 10411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2603 3425 3554 3683 3733 6064 7085 7131 Size: 1364 822 129 129 50 2331 1021 46 Cuts at: 7131 7623 9071 1239 Size: 492 1448 2579 Fragments arranged by size: 2579 2331 1448 1364 1021 822 492 129 129 50 46 Bpu10I CC'TnA_GC Cuts at: 2554 6150 6819 2554 Size: 3596 669 6146 Fragments arranged by size: 6146 3596 669 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7457 8536 9401 9699 7457 Size: 1079 865 298 8169 Fragments arranged by size: 8169 1079 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5194 6056 9074 882 Size: 617 3695 862 3018 2219 Fragments arranged by size: 3695 3018 2219 862 617 BsaAI yAC'GTr Cuts at: 493 1204 3180 4363 8202 493 Size: 711 1976 1183 3839 2702 Fragments arranged by size: 3839 2702 1976 1183 711 BsaBI GATnn'nnATC Cuts at: 3789 4256 6597 7418 10405 3789 Size: 467 2341 821 2987 3795 Fragments arranged by size: 3795 2987 2341 821 467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 3769 3832 4767 Size: 53 83 186 936 504 1732 63 935 Cuts at: 4767 6365 8218 8600 9235 275 Size: 1598 1853 382 635 1451 Fragments arranged by size: 1853 1732 1598 1451 936 935 635 504 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 2373 3474 3636 4518 5442 5588 5755 6606 8844 Size: 1101 162 882 924 146 167 851 2238 Cuts at: 8844 9242 9516 9663 10074 2373 Size: 398 274 147 411 2710 Fragments arranged by size: 2710 2238 1101 924 882 851 411 398 274 167 162 147 146 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 2579 2609 2666 Size: 30 165 30 927 30 219 30 57 Cuts at: 2666 2696 2699 2729 3506 3536 3883 3913 4331 Size: 30 3 30 777 30 347 30 418 Cuts at: 4331 4361 7574 7604 1178 Size: 30 3213 30 3985 Fragments arranged by size: 3985 3213 927 777 418 347 219 165 57 30 30 30 30 30 30 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2555 2600 2671 3083 3221 3527 7082 Size: 1165 204 45 71 412 138 306 3555 Cuts at: 7082 7178 7493 7684 1186 Size: 96 315 191 3913 Fragments arranged by size: 3913 3555 1165 412 315 306 204 191 138 96 71 45 BseYI C'CCAG_C Cuts at: 4080 4456 4746 7105 9614 10110 4080 Size: 376 290 2359 2509 496 4381 Fragments arranged by size: 4381 2509 2359 496 376 290 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2127 2782 2910 3152 3214 4114 5294 Size: 573 391 655 128 242 62 900 1180 Cuts at: 5294 5780 6850 1163 Size: 486 1070 4724 Fragments arranged by size: 4724 1180 1070 900 655 573 486 391 242 128 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 7861 8622 9650 1540 Size: 275 186 1291 4569 761 1028 2301 Fragments arranged by size: 4569 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2119 2450 5619 6156 7120 8472 8557 9628 2119 Size: 331 3169 537 964 1352 85 1071 2902 Fragments arranged by size: 3169 2902 1352 1071 964 537 331 85 BsmI GAATG_Cn' Cuts at: 3415 5575 7922 8015 3415 Size: 2160 2347 93 5811 Fragments arranged by size: 5811 2347 2160 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7065 7571 7065 Size: 506 9905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2450 3103 3177 3542 3634 4216 Size: 399 329 331 653 74 365 92 582 Cuts at: 4216 4457 4649 5391 5619 5920 6156 7001 7120 Size: 241 192 742 228 301 236 845 119 Cuts at: 7120 7730 8472 8557 9628 1391 Size: 610 742 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 742 653 610 582 399 365 331 329 301 241 236 228 192 119 92 85 74 BspEI T'CCGG_A Cuts at: 2373 5588 10074 2373 Size: 3215 4486 2710 Fragments arranged by size: 4486 3215 2710 BspHI T'CATG_A Cuts at: 8300 10030 8300 Size: 1730 8681 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2141 3149 3349 4099 4499 5099 5118 5138 Size: 1297 1008 200 750 400 600 19 20 Cuts at: 5138 5889 6078 6634 7766 844 Size: 751 189 556 1132 3489 Fragments arranged by size: 3489 1297 1132 1008 751 750 600 556 400 200 189 20 19 BsrBI CCG'CTC Cuts at: 1394 1823 2262 2700 3375 5584 6511 8298 1394 Size: 429 439 438 675 2209 927 1787 3507 Fragments arranged by size: 3507 2209 1787 927 675 439 438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2484 3085 3414 3678 3777 5253 5863 Size: 1183 1236 601 329 264 99 1476 610 Cuts at: 5863 6420 6471 8906 9080 65 Size: 557 51 2435 174 1396 Fragments arranged by size: 2435 1476 1396 1236 1183 610 601 557 329 264 174 99 51 BsrFI r'CCGG_y Cuts at: 1700 1810 3051 3156 3291 4769 4920 4998 5755 Size: 110 1241 105 135 1478 151 78 757 Cuts at: 5755 9055 9242 1700 Size: 3300 187 2869 Fragments arranged by size: 3300 2869 1478 1241 757 187 151 135 110 105 78 BsrGI T'GTAC_A Cuts at: 96 7233 7353 96 Size: 7137 120 3154 Fragments arranged by size: 7137 3154 120 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 10314 BssSI C'ACGA_G Cuts at: 5867 8471 9483 5867 Size: 2604 1012 6795 Fragments arranged by size: 6795 2604 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2114 3364 5130 794 Size: 1320 1250 1766 6075 Fragments arranged by size: 6075 1766 1320 1250 BstBI TT'CG_AA Cuts at: 9215 9215 Size: 10411 BstEII G'GTnAC_C Cuts at: 1184 4480 1184 Size: 3296 7115 BstXI CCAn_nnnn'nTGG Cuts at: 2636 4850 2636 Size: 2214 8197 BstYI r'GATC_y Cuts at: 1972 3477 6579 6699 7741 8108 8493 8510 9962 Size: 1505 3102 120 1042 367 385 17 1452 Cuts at: 9962 10048 10060 10406 1972 Size: 86 12 346 1977 Fragments arranged by size: 3102 1977 1505 1452 1042 385 367 346 120 86 17 12 BstZ17I GTA'TAC Cuts at: 2204 5419 2204 Size: 3215 7196 Bsu36I CC'TnA_GG Cuts at: 4678 4678 Size: 10411 BtgI C'CryG_G Cuts at: 513 1065 3693 4608 6085 513 Size: 552 2628 915 1477 4839 Fragments arranged by size: 4839 2628 1477 915 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2202 2406 2436 2649 2769 2916 Size: 683 56 976 204 30 213 120 147 Cuts at: 2916 487 Size: 7982 Fragments arranged by size: 7982 976 683 213 204 147 120 56 30 BtsI GCAGTG_nn' Cuts at: 2021 2115 2159 2386 2435 2565 2629 3135 3140 Size: 94 44 227 49 130 64 506 5 Cuts at: 3140 3386 4330 5306 6312 7927 8721 8741 2021 Size: 246 944 976 1006 1615 794 20 3691 Fragments arranged by size: 3691 1615 1006 976 944 794 506 246 227 130 94 64 49 44 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10411 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2389 2424 2995 3030 6677 6712 6826 Size: 35 1825 35 571 35 3647 35 114 Cuts at: 6826 6861 529 Size: 35 4079 Fragments arranged by size: 4079 3647 1825 571 114 35 35 35 35 35 DraI TTT'AAA Cuts at: 7715 7715 Size: 10411 DraIII CAC_nnn'GTG Cuts at: 5021 5021 Size: 10411 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4982 7057 9418 809 Size: 507 3666 2075 2361 1802 Fragments arranged by size: 3666 2361 2075 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 3615 4153 Size: 54 1425 50 275 18 231 1554 538 Cuts at: 4153 4467 5407 6318 6655 7858 8747 10376 8 Size: 314 940 911 337 1203 889 1629 43 Fragments arranged by size: 1629 1554 1425 1203 940 911 889 538 337 314 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7858 1537 Size: 275 6046 4090 Fragments arranged by size: 6046 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2901 3800 4705 6080 6455 7593 8341 1353 Size: 1548 899 905 1375 375 1138 748 3423 Fragments arranged by size: 3423 1548 1375 1138 905 899 748 375 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2246 2843 8998 9372 9518 387 Size: 1064 138 657 597 6155 374 146 1280 Fragments arranged by size: 6155 1280 1064 657 597 374 146 138 EcoICRI GAG'CTC Cuts at: 6154 6154 Size: 10411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 3425 3554 3683 3733 6047 6064 Size: 393 971 822 129 129 50 2314 17 Cuts at: 6064 6305 7085 7131 7623 8474 9071 9858 10126 Size: 241 780 46 492 851 597 787 268 Cuts at: 10126 1239 Size: 1524 Fragments arranged by size: 2314 1524 971 851 822 787 780 597 492 393 268 241 129 129 50 46 17 EcoO109I rG'GnC_Cy Cuts at: 3547 3630 3919 4285 4743 5061 6517 7385 7498 Size: 83 289 366 458 318 1456 868 113 Cuts at: 7498 3547 Size: 6460 Fragments arranged by size: 6460 1456 868 458 366 318 289 113 83 EcoRI G'AATT_C Cuts at: 7720 8098 7720 Size: 378 10033 EcoRV GAT'ATC Cuts at: 6802 6802 Size: 10411 FspI TGC'GCA Cuts at: 2050 2441 4322 6597 8917 2050 Size: 391 1881 2275 2320 3544 Fragments arranged by size: 3544 2320 2275 1881 391 FspAI rTGC'GCAy Cuts at: 6597 6597 Size: 10411 HaeII r_GCGC'y Cuts at: 1678 2040 2570 2752 2869 2974 3835 4002 4087 Size: 362 530 182 117 105 861 167 85 Cuts at: 4087 4917 9558 1678 Size: 830 4641 2531 Fragments arranged by size: 4641 2531 861 830 530 362 182 167 117 105 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2598 2630 2666 2698 2765 Size: 32 135 32 1026 32 36 32 67 Cuts at: 2765 2797 3506 3538 3759 3791 3865 3897 4274 Size: 32 709 32 221 32 74 32 377 Cuts at: 4274 4306 4331 4363 4727 4759 4860 4892 6034 Size: 32 25 32 364 32 101 32 1142 Cuts at: 6034 6066 6734 6766 7149 7181 7523 7555 9052 Size: 32 668 32 383 32 342 32 1497 Cuts at: 9052 9084 9126 9158 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1497 1142 1026 709 668 383 377 364 342 221 135 101 74 67 42 36 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3007 3085 6742 7755 8001 669 Size: 988 281 1069 78 3657 1013 246 3079 Fragments arranged by size: 3657 3079 1069 1013 988 281 246 78 HindIII A'AGCT_T Cuts at: 748 748 Size: 10411 HpaI GTT'AAC Cuts at: 3085 8001 3085 Size: 4916 5495 KasI G'GCGC_C Cuts at: 2036 3831 2036 Size: 1795 8616 KpnI G_GTAC'C Cuts at: 3705 5228 6448 6962 7736 3705 Size: 1523 1220 514 774 6380 Fragments arranged by size: 6380 1523 1220 774 514 MfeI C'AATT_G Cuts at: 4782 8010 4782 Size: 3228 7183 MluI A'CGCG_T Cuts at: 10287 10287 Size: 10411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2069 2475 2942 5242 5901 9525 9709 1305 Size: 764 406 467 2300 659 3624 184 2007 Fragments arranged by size: 3624 2300 2007 764 659 467 406 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 3617 4155 4469 6320 Size: 54 1425 343 231 1554 538 314 1851 Cuts at: 6320 10 Size: 4101 Fragments arranged by size: 4101 1851 1554 1425 538 343 314 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2391 2634 2807 3944 4209 4598 Size: 648 1021 204 243 173 1137 265 389 Cuts at: 4598 5183 5240 5769 5867 5897 6129 6131 6212 Size: 585 57 529 98 30 232 2 81 Cuts at: 6212 8369 8728 8887 518 Size: 2157 359 159 2042 Fragments arranged by size: 2157 2042 1137 1021 648 585 529 389 359 265 243 232 204 173 159 98 81 57 30 2 MspA1I CmG'CkG Cuts at: 1606 2466 2832 2847 3300 4107 4460 5085 6025 Size: 860 366 15 453 807 353 625 940 Cuts at: 6025 6823 7363 7588 7827 8504 9652 9897 1606 Size: 798 540 225 239 677 1148 245 2120 Fragments arranged by size: 2120 1148 940 860 807 798 677 625 540 453 366 353 245 239 225 15 NaeI GCC'GGC Cuts at: 3053 4771 5000 3053 Size: 1718 229 8464 Fragments arranged by size: 8464 1718 229 NarI GG'CG_CC Cuts at: 2037 3832 2037 Size: 1795 8616 NcoI C'CATG_G Cuts at: 513 1065 3693 4608 513 Size: 552 2628 915 6316 Fragments arranged by size: 6316 2628 915 552 NdeI CA'TA_TG Cuts at: 387 5770 387 Size: 5383 5028 NgoMIV G'CCGG_C Cuts at: 3051 4769 4998 3051 Size: 1718 229 8464 Fragments arranged by size: 8464 1718 229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10411 NotI GC'GGCC_GC Cuts at: 7858 7858 Size: 10411 NsiI A_TGCA'T Cuts at: 7523 7523 Size: 10411 NspI r_CATG'y Cuts at: 1132 1229 2106 2440 4624 5902 6140 7769 9314 Size: 97 877 334 2184 1278 238 1629 1545 Cuts at: 9314 1132 Size: 2229 Fragments arranged by size: 2229 2184 1629 1545 1278 877 334 238 97 PasI CC'CwG_GG Cuts at: 1348 3542 1348 Size: 2194 8217 PciI A'CATG_T Cuts at: 2102 4620 9310 2102 Size: 2518 4690 3203 Fragments arranged by size: 4690 3203 2518 PflMI CCAn_nnn'nTGG Cuts at: 1108 3711 4476 5536 1108 Size: 2603 765 1060 5983 Fragments arranged by size: 5983 2603 1060 765 PfoI T'CCnGG_A Cuts at: 6219 7264 7506 6219 Size: 1045 242 9124 Fragments arranged by size: 9124 1045 242 PmeI GTTT'AAAC Cuts at: 7715 7715 Size: 10411 PmlI CAC'GTG Cuts at: 3180 4363 3180 Size: 1183 9228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2569 7674 7706 8480 8512 9993 10025 2537 Size: 32 5105 32 774 32 1481 32 2923 Fragments arranged by size: 5105 2923 1481 774 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4285 4743 5061 6517 4285 Size: 458 318 1456 8179 Fragments arranged by size: 8179 1456 458 318 PshAI GACnn'nnGTC Cuts at: 1613 4260 4376 6345 1613 Size: 2647 116 1969 5679 Fragments arranged by size: 5679 2647 1969 116 PsiI TTA'TAA Cuts at: 7981 7981 Size: 10411 PspOMI G'GGCC_C Cuts at: 3630 3630 Size: 10411 PspXI vC'TCGA_Gb Cuts at: 3601 3601 Size: 10411 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7268 7300 7268 Size: 32 10379 PstI C_TGCA'G Cuts at: 830 4096 4953 7633 7763 830 Size: 3266 857 2680 130 3478 Fragments arranged by size: 3478 3266 2680 857 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10411 PvuII CAG'CTG Cuts at: 3300 4460 5085 6025 7363 7588 3300 Size: 1160 625 940 1338 225 6123 Fragments arranged by size: 6123 1338 1160 940 625 225 RsrII CG'GwC_CG Cuts at: 5586 5586 Size: 10411 SacI G_AGCT'C Cuts at: 6156 6156 Size: 10411 SalI G'TCGA_C Cuts at: 1936 7753 1936 Size: 5817 4594 SanDI GG'GwC_CC Cuts at: 4743 4743 Size: 10411 SapI GCTCTTCn'nnn_ Cuts at: 6080 7593 6080 Size: 1513 8898 SbfI CC_TGCA'GG Cuts at: 4096 7763 4096 Size: 3667 6744 ScaI AGT'ACT Cuts at: 4560 6396 8659 10371 4560 Size: 1836 2263 1712 4600 Fragments arranged by size: 4600 2263 1836 1712 SfcI C'TryA_G Cuts at: 826 1045 2467 4092 4949 5041 5372 7285 7629 Size: 219 1422 1625 857 92 331 1913 344 Cuts at: 7629 7759 8894 9575 9766 826 Size: 130 1135 681 191 1471 Fragments arranged by size: 1913 1625 1471 1422 1135 857 681 344 331 219 191 130 92 SfoI GGC'GCC Cuts at: 2038 3833 2038 Size: 1795 8616 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10411 SgrAI Cr'CCGG_yG Cuts at: 1700 5755 1700 Size: 4055 6356 SmaI CCC'GGG Cuts at: 1872 2327 3674 4352 5513 7738 1872 Size: 455 1347 678 1161 2225 4545 Fragments arranged by size: 4545 2225 1347 1161 678 455 SmlI C'TyrA_G Cuts at: 820 1017 1943 3601 5947 7472 8515 9416 9678 Size: 197 926 1658 2346 1525 1043 901 262 Cuts at: 9678 820 Size: 1553 Fragments arranged by size: 2346 1658 1553 1525 1043 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 8202 493 Size: 7709 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10411 SphI G_CATG'C Cuts at: 1132 7769 1132 Size: 6637 3774 SspI AAT'ATT Cuts at: 5 52 8335 5 Size: 47 8283 2081 Fragments arranged by size: 8283 2081 47 StuI AGG'CCT Cuts at: 1517 3746 5889 1517 Size: 2229 2143 6039 Fragments arranged by size: 6039 2229 2143 StyI C'CwwG_G Cuts at: 513 1065 2943 3693 4608 5632 6971 7404 7850 Size: 552 1878 750 915 1024 1339 433 446 Cuts at: 7850 513 Size: 3074 Fragments arranged by size: 3074 1878 1339 1024 915 750 552 446 433 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 3602 3835 4860 5781 6304 6577 Size: 1255 677 1345 233 1025 921 523 273 Cuts at: 6577 6884 8438 8455 8608 8793 9238 325 Size: 307 1554 17 153 185 445 1498 Fragments arranged by size: 1554 1498 1345 1255 1025 921 677 523 445 307 273 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3381 3840 3963 Size: 274 80 33 51 1443 1404 459 123 Cuts at: 3963 4365 4494 4558 5289 5547 6330 6394 7233 Size: 402 129 64 731 258 783 64 839 Cuts at: 7233 7353 8657 10369 96 Size: 120 1304 1712 138 Fragments arranged by size: 1712 1443 1404 1304 839 783 731 459 402 274 258 138 129 123 120 80 64 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2699 2731 3469 3501 4413 4445 5308 Size: 32 88 32 738 32 912 32 863 Cuts at: 5308 5340 5640 5672 6031 6063 6601 6633 7232 Size: 32 300 32 359 32 538 32 599 Cuts at: 7232 7264 10371 10403 2579 Size: 32 3107 32 2587 Fragments arranged by size: 3107 2587 912 863 738 599 538 359 300 88 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6724 1316 Size: 5408 5003 XbaI T'CTAG_A Cuts at: 7747 7747 Size: 10411 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2600 6298 7553 7668 2600 Size: 3698 1255 115 5343 Fragments arranged by size: 5343 3698 1255 115 XhoI C'TCGA_G Cuts at: 1943 3601 5947 1943 Size: 1658 2346 6407 Fragments arranged by size: 6407 2346 1658 XmaI C'CCGG_G Cuts at: 1870 2325 3672 4350 5511 7736 1870 Size: 455 1347 678 1161 2225 4545 Fragments arranged by size: 4545 2225 1347 1161 678 455 XmnI GAAnn'nnTTC Cuts at: 4032 5150 7158 8540 4032 Size: 1118 2008 1382 5903 Fragments arranged by size: 5903 2008 1382 1118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8219 276 Size: 53 83 186 936 6685 2468 Fragments arranged by size: 6685 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BsiWI EcoNI FalI FseI NruI PacI SacII SexAI SfiI SrfI SwaI