Restriction map for
pFN21AA0816
(Linear) (Six-base) MAPSORT of: KIAA0816.seq Check: 3005 from: 1 to: 5706
KIAA0816.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1344 5706
Size: 1344 4362
AccI GT'mk_AC
Cuts at: 0 198 1519 3007 3263 3413 5706
Size: 198 1321 1488 256 150 2293
Fragments arranged by size:
2293 1488 1321 256 198 150
Acc65I G'GTAC_C
Cuts at: 0 1696 3219 4439 4953 5706
Size: 1696 1523 1220 514 753
Fragments arranged by size:
1696 1523 1220 753 514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4042 4300 5706
Size: 4042 258 1406
Fragments arranged by size:
4042 1406 258
AfeI AGC'GCT
Cuts at: 0 1995 2910 5706
Size: 1995 915 2796
Fragments arranged by size:
2796 1995 915
AflIII A'CryG_T
Cuts at: 0 97 1172 2357 2615 5706
Size: 97 1075 1185 258 3091
Fragments arranged by size:
3091 1185 1075 258 97
AgeI A'CCGG_T
Cuts at: 0 3750 5706
Size: 3750 1956
AhdI GACnn_n'nnGTC
Cuts at: 0 5532 5706
Size: 5532 174
AleI CACnn'nnGTG
Cuts at: 0 2204 4126 4207 5706
Size: 2204 1922 81 1499
Fragments arranged by size:
2204 1922 1499 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3121 5706
Size: 3121 2585
AlwNI CAG_nnn'CTG
Cuts at: 0 951 1318 1359 1656 5706
Size: 951 367 41 297 4050
Fragments arranged by size:
4050 951 367 297 41
ApaI G_GGCC'C
Cuts at: 0 1629 5706
Size: 1629 4077
ApaLI G'TGCA_C
Cuts at: 0 5111 5706
Size: 5111 595
ApoI r'AATT_y
Cuts at: 0 2002 2782 5706
Size: 2002 780 2924
Fragments arranged by size:
2924 2002 780
AvaI C'yCGr_G
Cuts at: 0 67 320 1596 1667 2345 2582 3506 3942
Size: 67 253 1276 71 678 237 924 436
Cuts at: 3942 5706
Size: 1764
Fragments arranged by size:
1764 1276 924 678 436 253 237 71
67
AvrII C'CTAG_G
Cuts at: 0 3627 4966 5706
Size: 3627 1339 740
Fragments arranged by size:
3627 1339 740
BamHI G'GATC_C
Cuts at: 0 1472 5706
Size: 1472 4234
BanI G'GyrC_C
Cuts at: 0 31 1591 1696 1826 3219 3724 4439 4953
Size: 31 1560 105 130 1393 505 715 514
Cuts at: 4953 5706
Size: 753
Fragments arranged by size:
1560 1393 753 715 514 505 130 105
31
BanII G_rGCy'C
Cuts at: 0 1537 1629 2452 2644 3386 4151 4996 5706
Size: 1537 92 823 192 742 765 845 710
Fragments arranged by size:
1537 845 823 765 742 710 192 92
BbeI G_GCGC'C
Cuts at: 0 35 1830 5706
Size: 35 1795 3876
Fragments arranged by size:
3876 1795 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1437 1955 2779 2973 4042 4288 5527 5706
Size: 1437 518 824 194 1069 246 1239 179
Fragments arranged by size:
1437 1239 1069 824 518 246 194 179
BbvCI CC'TCA_GC
Cuts at: 0 549 4814 5706
Size: 549 4265 892
Fragments arranged by size:
4265 892 549
BciVI GTATCCnnnnn_n'
Cuts at: 0 2427 5706
Size: 2427 3279
BclI T'GATC_A
Cuts at: 0 4394 5706
Size: 4394 1312
BfrBI ATG'CAT
Cuts at: 0 5516 5706
Size: 5516 190
BglI GCCn_nnn'nGGC
Cuts at: 0 356 1145 1287 1541 2177 2456 3095 4010
Size: 356 789 142 254 636 279 639 915
Cuts at: 4010 5706
Size: 1696
Fragments arranged by size:
1696 915 789 639 636 356 279 254
142
BlpI GC'TnA_GC
Cuts at: 0 485 1850 5706
Size: 485 1365 3856
Fragments arranged by size:
3856 1365 485
Bme1580I G_kGCm'C
Cuts at: 0 1098 1172 1629 2211 3915 5115 5706
Size: 1098 74 457 582 1704 1200 591
Fragments arranged by size:
1704 1200 1098 591 582 457 74
BmgBI CAC'GTC
Cuts at: 0 2313 5054 5706
Size: 2313 2741 652
Fragments arranged by size:
2741 2313 652
BmrI ACTGGGnnnn_n'
Cuts at: 0 135 1532 1725 1848 2138 2346 2640 2763
Size: 135 1397 193 123 290 208 294 123
Cuts at: 2763 2995 3564 4317 4467 4590 4990 5706
Size: 232 569 753 150 123 400 716
Fragments arranged by size:
1397 753 716 569 400 294 290 232
208 193 150 135 123 123 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 5706
Size: 312 5394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1420 1549 1678 1728 4059 5080 5126
Size: 598 822 129 129 50 2331 1021 46
Cuts at: 5126 5618 5706
Size: 492 88
Fragments arranged by size:
2331 1021 822 598 492 129 129 88
50 46
Bpu10I CC'TnA_GC
Cuts at: 0 549 4145 4814 5706
Size: 549 3596 669 892
Fragments arranged by size:
3596 892 669 549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5452 5706
Size: 5452 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 3189 4051 5706
Size: 3189 862 1655
Fragments arranged by size:
3189 1655 862
BsaAI yAC'GTr
Cuts at: 0 1175 2358 5706
Size: 1175 1183 3348
Fragments arranged by size:
3348 1183 1175
BsaBI GATnn'nnATC
Cuts at: 0 1784 2251 4592 5413 5706
Size: 1784 467 2341 821 293
Fragments arranged by size:
2341 1784 821 467 293
BsaHI Gr'CG_yC
Cuts at: 0 32 1764 1827 2762 4360 5706
Size: 32 1732 63 935 1598 1346
Fragments arranged by size:
1732 1598 1346 935 63 32
BsaWI w'CCGG_w
Cuts at: 0 368 1469 1631 2513 3437 3583 3750 4601
Size: 368 1101 162 882 924 146 167 851
Cuts at: 4601 5706
Size: 1105
Fragments arranged by size:
1105 1101 924 882 851 368 167 162
146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 574 604 661 691 694 724
Size: 325 30 219 30 57 30 3 30
Cuts at: 724 1501 1531 1878 1908 2326 2356 5569 5599
Size: 777 30 347 30 418 30 3213 30
Cuts at: 5599 5706
Size: 107
Fragments arranged by size:
3213 777 418 347 325 219 107 57
30 30 30 30 30 30 30 30
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 550 595 666 1078 1216 1522 5077
Size: 346 204 45 71 412 138 306 3555
Cuts at: 5077 5173 5488 5679 5706
Size: 96 315 191 27
Fragments arranged by size:
3555 412 346 315 306 204 191 138
96 71 45 27
BseYI C'CCAG_C
Cuts at: 0 2075 2451 2741 5100 5706
Size: 2075 376 290 2359 606
Fragments arranged by size:
2359 2075 606 376 290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 777 905 1147 1209 2109 3289 3775
Size: 122 655 128 242 62 900 1180 486
Cuts at: 3775 4845 5706
Size: 1070 861
Fragments arranged by size:
1180 1070 900 861 655 486 242 128
122 62
BsiEI CG_ry'CG
Cuts at: 0 1287 5706
Size: 1287 4419
BsiHKAI G_wGCw'C
Cuts at: 0 114 445 3614 4151 5115 5706
Size: 114 331 3169 537 964 591
Fragments arranged by size:
3169 964 591 537 331 114
BsmI GAATG_Cn'
Cuts at: 0 1410 3570 5706
Size: 1410 2160 2136
Fragments arranged by size:
2160 2136 1410
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5060 5566 5706
Size: 5060 506 140
Fragments arranged by size:
5060 506 140
Bsp1286I G_dGCh'C
Cuts at: 0 114 445 1098 1172 1537 1629 2211 2452
Size: 114 331 653 74 365 92 582 241
Cuts at: 2452 2644 3386 3614 3915 4151 4996 5115 5706
Size: 192 742 228 301 236 845 119 591
Fragments arranged by size:
845 742 653 591 582 365 331 301
241 236 228 192 119 114 92 74
BspEI T'CCGG_A
Cuts at: 0 368 3583 5706
Size: 368 3215 2123
Fragments arranged by size:
3215 2123 368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 1144 1344 2094 2494 3094 3113 3133
Size: 136 1008 200 750 400 600 19 20
Cuts at: 3133 3884 4073 4629 5706
Size: 751 189 556 1077
Fragments arranged by size:
1077 1008 751 750 600 556 400 200
189 136 20 19
BsrBI CCG'CTC
Cuts at: 0 257 695 1370 3579 4506 5706
Size: 257 438 675 2209 927 1200
Fragments arranged by size:
2209 1200 927 675 438 257
BsrDI GCAATG_nn'
Cuts at: 0 479 1080 1409 1673 1772 3248 3858 4415
Size: 479 601 329 264 99 1476 610 557
Cuts at: 4415 4466 5706
Size: 51 1240
Fragments arranged by size:
1476 1240 610 601 557 479 329 264
99 51
BsrFI r'CCGG_y
Cuts at: 0 1046 1151 1286 2764 2915 2993 3750 5706
Size: 1046 105 135 1478 151 78 757 1956
Fragments arranged by size:
1956 1478 1046 757 151 135 105 78
BsrGI T'GTAC_A
Cuts at: 0 5228 5348 5706
Size: 5228 120 358
Fragments arranged by size:
5228 358 120
BssHII G'CGCG_C
Cuts at: 0 17 5706
Size: 17 5689
BssSI C'ACGA_G
Cuts at: 0 3862 5706
Size: 3862 1844
BstAPI GCAn_nnn'nTGC
Cuts at: 0 109 1359 3125 5706
Size: 109 1250 1766 2581
Fragments arranged by size:
2581 1766 1250 109
BstEII G'GTnAC_C
Cuts at: 0 2475 5706
Size: 2475 3231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 631 2845 5706
Size: 631 2214 2861
Fragments arranged by size:
2861 2214 631
BstYI r'GATC_y
Cuts at: 0 1472 4574 4694 5706
Size: 1472 3102 120 1012
Fragments arranged by size:
3102 1472 1012 120
BstZ17I GTA'TAC
Cuts at: 0 199 3414 5706
Size: 199 3215 2292
Fragments arranged by size:
3215 2292 199
Bsu36I CC'TnA_GG
Cuts at: 0 2673 5706
Size: 2673 3033
BtgI C'CryG_G
Cuts at: 0 1688 2603 4080 5706
Size: 1688 915 1477 1626
Fragments arranged by size:
1688 1626 1477 915
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 197 401 431 644 764 911 5706
Size: 197 204 30 213 120 147 4795
Fragments arranged by size:
4795 213 204 197 147 120 30
BtsI GCAGTG_nn'
Cuts at: 0 16 110 154 381 430 560 624 1130
Size: 16 94 44 227 49 130 64 506
Cuts at: 1130 1135 1381 2325 3301 4307 5706
Size: 5 246 944 976 1006 1399
Fragments arranged by size:
1399 1006 976 944 506 246 227 130
94 64 49 44 16 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 384 419 990 1025 4672 4707 4821 4856
Size: 384 35 571 35 3647 35 114 35
Cuts at: 4856 5706
Size: 850
Fragments arranged by size:
3647 850 571 384 114 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 3016 5706
Size: 3016 2690
DrdI GACnn_nn'nnGTC
Cuts at: 0 2977 5052 5706
Size: 2977 2075 654
Fragments arranged by size:
2977 2075 654
EaeI y'GGCC_r
Cuts at: 0 56 1610 2148 2462 3402 4313 4650 5706
Size: 56 1554 538 314 940 911 337 1056
Fragments arranged by size:
1554 1056 940 911 538 337 314 56
EarI CTCTTCn'nnn_
Cuts at: 0 896 1795 2700 4075 4450 5588 5706
Size: 896 899 905 1375 375 1138 118
Fragments arranged by size:
1375 1138 905 899 896 375 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 241 838 5706
Size: 241 597 4868
Fragments arranged by size:
4868 597 241
EcoICRI GAG'CTC
Cuts at: 0 4149 5706
Size: 4149 1557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1420 1549 1678 1728 4042 4059 4300
Size: 598 822 129 129 50 2314 17 241
Cuts at: 4300 5080 5126 5618 5706
Size: 780 46 492 88
Fragments arranged by size:
2314 822 780 598 492 241 129 129
88 50 46 17
EcoO109I rG'GnC_Cy
Cuts at: 0 1542 1625 1914 2280 2738 3056 4512 5380
Size: 1542 83 289 366 458 318 1456 868
Cuts at: 5380 5493 5706
Size: 113 213
Fragments arranged by size:
1542 1456 868 458 366 318 289 213
113 83
EcoRV GAT'ATC
Cuts at: 0 4797 5706
Size: 4797 909
FspI TGC'GCA
Cuts at: 0 45 436 2317 4592 5706
Size: 45 391 1881 2275 1114
Fragments arranged by size:
2275 1881 1114 391 45
FspAI rTGC'GCAy
Cuts at: 0 4592 5706
Size: 4592 1114
HaeII r_GCGC'y
Cuts at: 0 35 565 747 864 969 1830 1997 2082
Size: 35 530 182 117 105 861 167 85
Cuts at: 2082 2912 5706
Size: 830 2794
Fragments arranged by size:
2794 861 830 530 182 167 117 105
85 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 593 625 661 693 760 792 1501 1533
Size: 593 32 36 32 67 32 709 32
Cuts at: 1533 1754 1786 1860 1892 2269 2301 2326 2358
Size: 221 32 74 32 377 32 25 32
Cuts at: 2358 2722 2754 2855 2887 4029 4061 4729 4761
Size: 364 32 101 32 1142 32 668 32
Cuts at: 4761 5144 5176 5518 5550 5706
Size: 383 32 342 32 156
Fragments arranged by size:
1142 709 668 593 383 377 364 342
221 156 101 74 67 36 32 32
32 32 32 32 32 32 32 32
32 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1002 1080 4737 5706
Size: 1002 78 3657 969
Fragments arranged by size:
3657 1002 969 78
HpaI GTT'AAC
Cuts at: 0 1080 5706
Size: 1080 4626
KasI G'GCGC_C
Cuts at: 0 31 1826 5706
Size: 31 1795 3880
Fragments arranged by size:
3880 1795 31
KpnI G_GTAC'C
Cuts at: 0 1700 3223 4443 4957 5706
Size: 1700 1523 1220 514 749
Fragments arranged by size:
1700 1523 1220 749 514
MfeI C'AATT_G
Cuts at: 0 2777 5706
Size: 2777 2929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 470 937 3237 3896 5706
Size: 64 406 467 2300 659 1810
Fragments arranged by size:
2300 1810 659 467 406 64
MscI TGG'CCA
Cuts at: 0 58 1612 2150 2464 4315 5706
Size: 58 1554 538 314 1851 1391
Fragments arranged by size:
1851 1554 1391 538 314 58
MslI CAynn'nnrTG
Cuts at: 0 182 386 629 802 1939 2204 2593 3178
Size: 182 204 243 173 1137 265 389 585
Cuts at: 3178 3235 3764 3862 3892 4124 4126 4207 5706
Size: 57 529 98 30 232 2 81 1499
Fragments arranged by size:
1499 1137 585 529 389 265 243 232
204 182 173 98 81 57 30 2
MspA1I CmG'CkG
Cuts at: 0 461 827 842 1295 2102 2455 3080 4020
Size: 461 366 15 453 807 353 625 940
Cuts at: 4020 4818 5358 5583 5706
Size: 798 540 225 123
Fragments arranged by size:
940 807 798 625 540 461 453 366
353 225 123 15
NaeI GCC'GGC
Cuts at: 0 1048 2766 2995 5706
Size: 1048 1718 229 2711
Fragments arranged by size:
2711 1718 1048 229
NarI GG'CG_CC
Cuts at: 0 32 1827 5706
Size: 32 1795 3879
Fragments arranged by size:
3879 1795 32
NcoI C'CATG_G
Cuts at: 0 1688 2603 5706
Size: 1688 915 3103
Fragments arranged by size:
3103 1688 915
NdeI CA'TA_TG
Cuts at: 0 3765 5706
Size: 3765 1941
NgoMIV G'CCGG_C
Cuts at: 0 1046 2764 2993 5706
Size: 1046 1718 229 2713
Fragments arranged by size:
2713 1718 1046 229
NsiI A_TGCA'T
Cuts at: 0 5518 5706
Size: 5518 188
NspI r_CATG'y
Cuts at: 0 101 435 2619 3897 4135 5706
Size: 101 334 2184 1278 238 1571
Fragments arranged by size:
2184 1571 1278 334 238 101
PasI CC'CwG_GG
Cuts at: 0 1537 5706
Size: 1537 4169
PciI A'CATG_T
Cuts at: 0 97 2615 5706
Size: 97 2518 3091
Fragments arranged by size:
3091 2518 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 1706 2471 3531 5706
Size: 1706 765 1060 2175
Fragments arranged by size:
2175 1706 1060 765
PfoI T'CCnGG_A
Cuts at: 0 4214 5259 5501 5706
Size: 4214 1045 242 205
Fragments arranged by size:
4214 1045 242 205
PmlI CAC'GTG
Cuts at: 0 1175 2358 5706
Size: 1175 1183 3348
Fragments arranged by size:
3348 1183 1175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 5669 5701 5706
Size: 532 32 5105 32 5
Fragments arranged by size:
5105 532 32 32 5
PpuMI rG'GwC_Cy
Cuts at: 0 2280 2738 3056 4512 5706
Size: 2280 458 318 1456 1194
Fragments arranged by size:
2280 1456 1194 458 318
PshAI GACnn'nnGTC
Cuts at: 0 2255 2371 4340 5706
Size: 2255 116 1969 1366
Fragments arranged by size:
2255 1969 1366 116
PspOMI G'GGCC_C
Cuts at: 0 1625 5706
Size: 1625 4081
PspXI vC'TCGA_Gb
Cuts at: 0 1596 5706
Size: 1596 4110
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5263 5295 5706
Size: 5263 32 411
Fragments arranged by size:
5263 411 32
PstI C_TGCA'G
Cuts at: 0 2091 2948 5628 5706
Size: 2091 857 2680 78
Fragments arranged by size:
2680 2091 857 78
PvuII CAG'CTG
Cuts at: 0 1295 2455 3080 4020 5358 5583 5706
Size: 1295 1160 625 940 1338 225 123
Fragments arranged by size:
1338 1295 1160 940 625 225 123
RsrII CG'GwC_CG
Cuts at: 0 3581 5706
Size: 3581 2125
SacI G_AGCT'C
Cuts at: 0 4151 5706
Size: 4151 1555
SanDI GG'GwC_CC
Cuts at: 0 2738 5706
Size: 2738 2968
SapI GCTCTTCn'nnn_
Cuts at: 0 4075 5588 5706
Size: 4075 1513 118
Fragments arranged by size:
4075 1513 118
SbfI CC_TGCA'GG
Cuts at: 0 2091 5706
Size: 2091 3615
ScaI AGT'ACT
Cuts at: 0 2555 4391 5706
Size: 2555 1836 1315
Fragments arranged by size:
2555 1836 1315
SfcI C'TryA_G
Cuts at: 0 462 2087 2944 3036 3367 5280 5624 5706
Size: 462 1625 857 92 331 1913 344 82
Fragments arranged by size:
1913 1625 857 462 344 331 92 82
SfoI GGC'GCC
Cuts at: 0 33 1828 5706
Size: 33 1795 3878
Fragments arranged by size:
3878 1795 33
SgrAI Cr'CCGG_yG
Cuts at: 0 3750 5706
Size: 3750 1956
SmaI CCC'GGG
Cuts at: 0 322 1669 2347 3508 5706
Size: 322 1347 678 1161 2198
Fragments arranged by size:
2198 1347 1161 678 322
SmlI C'TyrA_G
Cuts at: 0 1596 3942 5467 5706
Size: 1596 2346 1525 239
Fragments arranged by size:
2346 1596 1525 239
StuI AGG'CCT
Cuts at: 0 1741 3884 5706
Size: 1741 2143 1822
Fragments arranged by size:
2143 1822 1741
StyI C'CwwG_G
Cuts at: 0 938 1688 2603 3627 4966 5399 5706
Size: 938 750 915 1024 1339 433 307
Fragments arranged by size:
1339 1024 938 915 750 433 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 1597 1830 2855 3776 4299 4572 4879
Size: 252 1345 233 1025 921 523 273 307
Cuts at: 4879 5706
Size: 827
Fragments arranged by size:
1345 1025 921 827 523 307 273 252
233
TatI w'GTAC_w
Cuts at: 0 1376 1835 1958 2360 2489 2553 3284 3542
Size: 1376 459 123 402 129 64 731 258
Cuts at: 3542 4325 4389 5228 5348 5706
Size: 783 64 839 120 358
Fragments arranged by size:
1376 839 783 731 459 402 358 258
129 123 120 64 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 694 726 1464 1496 2408 2440
Size: 574 32 88 32 738 32 912 32
Cuts at: 2440 3303 3335 3635 3667 4026 4058 4596 4628
Size: 863 32 300 32 359 32 538 32
Cuts at: 4628 5227 5259 5706
Size: 599 32 447
Fragments arranged by size:
912 863 738 599 574 538 447 359
300 88 32 32 32 32 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4719 5706
Size: 4719 987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 595 4293 5548 5663 5706
Size: 595 3698 1255 115 43
Fragments arranged by size:
3698 1255 595 115 43
XhoI C'TCGA_G
Cuts at: 0 1596 3942 5706
Size: 1596 2346 1764
Fragments arranged by size:
2346 1764 1596
XmaI C'CCGG_G
Cuts at: 0 320 1667 2345 3506 5706
Size: 320 1347 678 1161 2200
Fragments arranged by size:
2200 1347 1161 678 320
XmnI GAAnn'nnTTC
Cuts at: 0 2027 3145 5153 5706
Size: 2027 1118 2008 553
Fragments arranged by size:
2027 2008 1118 553
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII RsrII SacI SanDI SapI
SbfI ScaI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AflII AloI AscI AseI AsiSI BaeI
BcgI BglII BmtI BsiWI BspHI BstBI ClaI DraI
EagI EcoNI EcoRI FalI FseI HindIII MluI NheI
NotI NruI PacI PmeI PsiI PvuI SacII SalI
SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI XbaI ZraI