Restriction map for
pFN21AA0802
##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AflIII AgeI AscI AspI AvrII
BbrPI BciVI BglII BlnI BsaAI
BsaBI BsiWI BspDI BspHI BspLU11I
BsrDI Bst1107I BstZ17I ClaI CpoI
DraI DraIII EcoRV EcoT22I HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII ScaI SexAI SfiI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 3341 GGTACC G^GTACC
AccI 3481 GTCGAC GT^[AC][GT]AC
AccIII 863 TCCGGA T^CCGGA
AclI 2797 AACGTT AA^CGTT
AcsI 2713 GAATTC [GA]^AATT[CT]
AflII 235 CTTAAG C^TTAAG
AhdI 1723 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1100 AGCGCT AGC^GCT
ApoI 2713 GAATTC [GA]^AATT[CT]
AseI 4192 ATTAAT AT^TAAT
AsnI 4192 ATTAAT AT^TAAT
Asp718I 3341 GGTACC G^GTACC
AspEI 1723 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4472 GGATCC G^GATCC
BbeI 3638 GGCGCC GGCGC^C
BbvCI 265 GCTGAGG CCTCAGC(-5/-2)
BclI 2781 TGATCA T^GATCA
BfrI 235 CTTAAG C^TTAAG
BseAI 863 TCCGGA T^CCGGA
Bsp1407I 389 TGTACA T^GTACA
BspEI 863 TCCGGA T^CCGGA
BsrGI 389 TGTACA T^GTACA
BssHII 255 GCGCGC G^CGCGC
BstBI 173 TTCGAA TT^CGAA
BstEII 2170 GGTCACC G^GT[ACGT]ACC
BstPI 2170 GGTCACC G^GT[ACGT]ACC
EagI 2893 CGGCCG C^GGCCG
Eam1105I 1723 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2893 CGGCCG C^GGCCG
Eco47III 1100 AGCGCT AGC^GCT
Eco52I 2893 CGGCCG C^GGCCG
EcoO65I 2170 GGTCACC G^GT[ACGT]ACC
EcoRI 2713 GAATTC G^AATTC
FbaI 2781 TGATCA T^GATCA
FseI 1766 GGCCGGCC GGCCGG^CC
HgaI 3099 GCGTC GACGC(5/10)
HincII 3481 GTCGAC GT[CT]^[GA]AC
HindII 3481 GTCGAC GT[CT]^[GA]AC
KasI 3638 GGCGCC G^GCGCC
KpnI 3341 GGTACC GGTAC^C
KspI 2285 CCGCGG CCGC^GG
MroI 863 TCCGGA T^CCGGA
NarI 3638 GGCGCC GG^CGCC
NcoI 4592 CCATGG C^CATGG
NspV 173 TTCGAA TT^CGAA
PshBI 4192 ATTAAT AT^TAAT
Psp1406I 2797 AACGTT AA^CGTT
SacII 2285 CCGCGG CCGC^GG
SalI 3481 GTCGAC G^TCGAC
SapI 2980 GAAGAGC GCTCTTC(1/4)
SbfI 999 CCTGCAGG CCTGCA^GG
SfoI 3638 GGCGCC GGC^GCC
SfuI 173 TTCGAA TT^CGAA
SphI 2831 GCATGC GCATG^C
Sse8387I 999 CCTGCAGG CCTGCA^GG
SspBI 389 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1272 GACGTC GACGT^C
4104 GACGTC
Alw44I 2563 GTGCAC G^TGCAC
4180 GTGCAC
AocI 3028 CCTGAGG CC^T[ACGT]AGG
3969 CCTTAGG
ApaLI 2563 GTGCAC G^TGCAC
4180 GTGCAC
AviII 476 TGCGCA TGC^GCA
3473 TGCGCA
BalI 488 TGGCCA TGG^CCA
2846 TGGCCA
BcgI 1161 CGAGAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
48 GCAGGAACTTCG
BsaWI 863 TCCGGA [AT]^CCGG[AT]
2009 TCCGGT
BsiEI 2667 CGACCG CG[GA][CT]^CG
2893 CGGCCG
BsmBI 3953 CGTCTC CGTCTC(1/5)
145 GAGACG
BsmI 3753 GAATGC GAATGC(1/-1)
773 GCATTC
BstAPI 3405 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4025 GCACATGCTGC
BstYI 2618 AGATCC [GA]^GATC[CT]
4472 GGATCC
Bsu36I 3028 CCTGAGG CC^T[ACGT]AGG
3969 CCTTAGG
DrdI 1668 GACTTCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3807 GACCAATGGGTC
Eco81I 3028 CCTGAGG CC^T[ACGT]AGG
3969 CCTTAGG
EcoT14I 3554 CCAAGG C^C[AT][AT]GG
4592 CCATGG
FspI 476 TGCGCA TGC^GCA
3473 TGCGCA
MflI 2618 AGATCC [GA]^GATC[CT]
4472 GGATCC
MluNI 488 TGGCCA TGG^CCA
2846 TGGCCA
MscI 488 TGGCCA TGG^CCA
2846 TGGCCA
NaeI 1767 GCCGGC GCC^GGC
4273 GCCGGC
NgoMIV 1767 GCCGGC G^CCGGC
4273 GCCGGC
StuI 1580 AGGCCT AGG^CCT
2348 AGGCCT
StyI 3554 CCAAGG C^C[AT][AT]GG
4592 CCATGG
Tsp509I 2714 AATT ^AATT
4339 AATT
XcmI 1691 CCATCGGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2842 CCAGTGGCCATGTGG
XhoII 2618 AGATCC [GA]^GATC[CT]
4472 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XmaCI
XmaI XmnI