(Circular) (Six-base) MAPSORT of: pFN21AA0802.seq Check: 6778 from: 1 to: 9340 pFN21AA0802 9340 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2844 3589 5495 5580 2844 Size: 745 1906 85 6604 Fragments arranged by size: 6604 1906 745 85 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3281 6113 7150 278 Size: 53 83 186 936 1745 2832 1037 2468 Fragments arranged by size: 2832 2468 1745 1037 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5487 6683 1937 Size: 3550 1196 4594 Fragments arranged by size: 4594 3550 1196 Acc65I G'GTAC_C Cuts at: 5346 6661 5346 Size: 1315 8025 AclI AA'CG_TT Cuts at: 1563 4803 7467 7840 1563 Size: 3240 2664 373 3063 Fragments arranged by size: 3240 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 3038 3359 3865 4934 5895 7403 8787 Size: 1013 393 321 506 1069 961 1508 1384 Cuts at: 8787 9055 1632 Size: 268 1917 Fragments arranged by size: 1917 1508 1384 1069 1013 961 506 393 321 268 AfeI AGC'GCT Cuts at: 3107 3107 Size: 9340 AflII C'TTAA_G Cuts at: 820 1017 2240 820 Size: 197 1223 7920 Fragments arranged by size: 7920 1223 197 AflIII A'CryG_T Cuts at: 1203 8239 9216 1203 Size: 7036 977 1327 Fragments arranged by size: 7036 1327 977 AgeI A'CCGG_T Cuts at: 8171 8171 Size: 9340 AhdI GACnn_n'nnGTC Cuts at: 3733 8069 3733 Size: 4336 5004 AleI CACnn'nnGTG Cuts at: 1166 4203 5256 6419 1166 Size: 3037 1053 1163 4087 Fragments arranged by size: 4087 3037 1163 1053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3927 4440 1184 Size: 2743 513 6084 Fragments arranged by size: 6084 2743 513 AlwNI CAG_nnn'CTG Cuts at: 3538 4336 4874 5416 7083 8655 3538 Size: 798 538 542 1667 1572 4223 Fragments arranged by size: 4223 1667 1572 798 542 538 ApaI G_GGCC'C Cuts at: 2601 2756 3001 3725 6157 6323 6543 6597 2601 Size: 155 245 724 2432 166 220 54 5344 Fragments arranged by size: 5344 2432 724 245 220 166 155 54 ApaLI G'TGCA_C Cuts at: 4568 6185 7397 8553 4568 Size: 1617 1212 1156 5355 Fragments arranged by size: 5355 1617 1212 1156 ApoI r'AATT_y Cuts at: 1493 4718 6649 6875 7027 9136 1493 Size: 3225 1931 226 152 2109 1697 Fragments arranged by size: 3225 2109 1931 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6198 7894 160 Size: 872 5166 1696 1606 Fragments arranged by size: 5166 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9340 AvaI C'yCGr_G Cuts at: 1870 1943 2385 2610 2755 2946 3150 3345 3684 Size: 73 442 225 145 191 204 195 339 Cuts at: 3684 3767 3777 4151 4251 5006 5548 5619 6348 Size: 83 10 374 100 755 542 71 729 Cuts at: 6348 6544 6665 1870 Size: 196 121 4545 Fragments arranged by size: 4545 755 729 542 442 374 339 225 204 196 195 191 145 121 100 83 73 71 10 BamHI G'GATC_C Cuts at: 6477 6670 7037 6477 Size: 193 367 8780 Fragments arranged by size: 8780 367 193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2442 2984 5346 5643 6338 Size: 325 217 627 655 542 2362 297 695 Cuts at: 6338 6661 8116 618 Size: 323 1455 1842 Fragments arranged by size: 2362 1842 1455 695 655 627 542 325 323 297 217 BanII G_rGCy'C Cuts at: 1391 2535 2601 2756 2826 2922 3001 3048 3336 Size: 1144 66 155 70 96 79 47 288 Cuts at: 3336 3531 3725 4014 5549 6157 6182 6306 6323 Size: 195 194 289 1535 608 25 124 17 Cuts at: 6323 6543 6597 6659 1391 Size: 220 54 62 4072 Fragments arranged by size: 4072 1535 1144 608 289 288 220 195 194 155 124 96 79 70 66 62 54 47 25 17 BbeI G_GCGC'C Cuts at: 5647 5647 Size: 9340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2105 2898 3281 4502 4899 928 Size: 971 206 793 383 1221 397 5369 Fragments arranged by size: 5369 1221 971 793 397 383 206 BbvCI CC'TCA_GC Cuts at: 2271 2271 Size: 9340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2042 2076 3155 3189 7531 7565 1903 Size: 34 105 34 1079 34 4342 34 3678 Fragments arranged by size: 4342 3678 1079 105 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7232 8442 7232 Size: 1210 8130 BclI T'GATC_A Cuts at: 1548 4786 1548 Size: 3238 6102 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2959 3492 4352 Size: 107 122 71 631 605 1287 533 860 Cuts at: 4352 7951 136 Size: 3599 1525 Fragments arranged by size: 3599 1525 1287 860 631 605 533 122 107 71 BglII A'GATC_T Cuts at: 9335 9335 Size: 9340 BlpI GC'TnA_GC Cuts at: 2921 3047 5585 6295 6757 2921 Size: 126 2538 710 462 5504 Fragments arranged by size: 5504 2538 710 462 126 Bme1580I G_kGCm'C Cuts at: 1790 2601 2756 3001 3142 3725 4402 4572 5310 Size: 811 155 245 141 583 677 170 738 Cuts at: 5310 5641 6157 6189 6323 6416 6543 6597 7401 Size: 331 516 32 134 93 127 54 804 Cuts at: 7401 8557 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 811 804 738 677 583 516 331 245 170 155 141 134 127 93 54 32 BmgBI CAC'GTC Cuts at: 1318 4450 5428 1318 Size: 3132 978 5230 Fragments arranged by size: 5230 3132 978 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 7071 8029 Size: 416 53 83 399 24 1721 3933 958 Cuts at: 8029 442 Size: 1753 Fragments arranged by size: 3933 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3271 4159 3271 Size: 888 8452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2318 2366 2435 2519 2543 2708 2828 Size: 968 111 48 69 84 24 165 120 Cuts at: 2828 3473 3614 3776 3908 4000 4129 4382 5172 Size: 645 141 162 132 92 129 253 790 Cuts at: 5172 5556 5723 6196 8000 1239 Size: 384 167 473 1804 2579 Fragments arranged by size: 2579 1804 968 790 645 473 384 253 167 165 162 141 132 129 120 111 92 84 69 48 24 Bpu10I CC'TnA_GC Cuts at: 2271 2271 Size: 9340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 3937 4451 5183 5740 7465 8330 8628 2693 Size: 1244 514 732 557 1725 865 298 3405 Fragments arranged by size: 3405 1725 1244 865 732 557 514 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4130 6294 6554 8003 882 Size: 617 2631 2164 260 1449 2219 Fragments arranged by size: 2631 2219 2164 1449 617 260 BsaAI yAC'GTr Cuts at: 493 1204 7131 493 Size: 711 5927 2702 Fragments arranged by size: 5927 2702 711 BsaBI GATnn'nnATC Cuts at: 9334 9334 Size: 9340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3278 5644 6110 7147 Size: 53 83 186 936 1745 2366 466 1037 Cuts at: 7147 7529 8164 275 Size: 382 635 1451 Fragments arranged by size: 2366 1745 1451 1037 936 635 466 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2868 4014 7773 8171 8445 8592 9003 2868 Size: 1146 3759 398 274 147 411 3205 Fragments arranged by size: 3759 3205 1146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2316 2346 3005 3035 3251 Size: 30 165 30 913 30 659 30 216 Cuts at: 3251 3281 4001 4031 1178 Size: 30 720 30 6487 Fragments arranged by size: 6487 913 720 659 216 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2663 3026 3215 3275 3515 3911 3914 4112 Size: 1477 363 189 60 240 396 3 198 Cuts at: 4112 4124 4516 5225 5329 6530 6535 1186 Size: 12 392 709 104 1201 5 3991 Fragments arranged by size: 3991 1477 1201 709 396 392 363 240 198 189 104 60 12 5 3 BseYI C'CCAG_C Cuts at: 2603 2774 3216 3387 3531 3538 3718 3862 5226 Size: 171 442 171 144 7 180 144 1364 Cuts at: 5226 5933 6316 6571 8543 9039 2603 Size: 707 383 255 1972 496 2904 Fragments arranged by size: 2904 1972 1364 707 496 442 383 255 180 171 171 144 144 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3809 4277 4887 5678 6032 6269 6412 Size: 573 2073 468 610 791 354 237 143 Cuts at: 6412 1163 Size: 4091 Fragments arranged by size: 4091 2073 791 610 573 468 354 237 143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4675 4901 6790 7551 8579 1540 Size: 275 186 2674 226 1889 761 1028 2301 Fragments arranged by size: 2674 2301 1889 1028 761 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2535 2826 2922 3048 3063 3336 3906 4014 4239 Size: 291 96 126 15 273 570 108 225 Cuts at: 4239 4467 4572 4934 5400 6189 7401 7486 8557 Size: 228 105 362 466 789 1212 85 1071 Cuts at: 8557 2535 Size: 3318 Fragments arranged by size: 3318 1212 1071 789 570 466 362 291 273 228 225 126 108 105 96 85 15 BsmI GAATG_Cn' Cuts at: 2778 5764 6851 6944 2778 Size: 2986 1087 93 5174 Fragments arranged by size: 5174 2986 1087 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2144 5964 2144 Size: 3820 5520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 2601 2756 2826 2922 3001 3048 Size: 399 745 66 155 70 96 79 47 Cuts at: 3048 3063 3142 3336 3531 3725 3906 4014 4239 Size: 15 79 194 195 194 181 108 225 Cuts at: 4239 4402 4467 4572 4934 5310 5400 5549 5641 Size: 163 65 105 362 376 90 149 92 Cuts at: 5641 6157 6182 6189 6306 6323 6416 6543 6597 Size: 516 25 7 117 17 93 127 54 Cuts at: 6597 6659 7401 7486 8557 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 516 399 376 362 225 195 194 194 181 163 155 149 127 117 108 105 96 93 92 90 85 79 79 70 66 65 62 54 47 25 17 15 7 BspEI T'CCGG_A Cuts at: 2868 9003 2868 Size: 6135 3205 BspHI T'CATG_A Cuts at: 7229 8959 7229 Size: 1730 7610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2844 3589 3884 4620 5495 5580 6695 844 Size: 2000 745 295 736 875 85 1115 3489 Fragments arranged by size: 3489 2000 1115 875 745 736 295 85 BsrBI CCG'CTC Cuts at: 1394 1823 2746 3294 3400 3505 3790 4705 6159 Size: 429 923 548 106 105 285 915 1454 Cuts at: 6159 7227 1394 Size: 1068 3507 Fragments arranged by size: 3507 1454 1068 923 915 548 429 285 106 105 BsrDI GCAATG_nn' Cuts at: 65 1248 7835 8009 65 Size: 1183 6587 174 1396 Fragments arranged by size: 6587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2901 3772 4896 6278 7984 8171 1700 Size: 110 1091 871 1124 1382 1706 187 2869 Fragments arranged by size: 2869 1706 1382 1124 1091 871 187 110 BsrGI T'GTAC_A Cuts at: 96 2394 96 Size: 2298 7042 BssHII G'CGCG_C Cuts at: 1925 2260 1925 Size: 335 9005 BssSI C'ACGA_G Cuts at: 2915 3074 3380 5309 7400 8412 2915 Size: 159 306 1929 2091 1012 3843 Fragments arranged by size: 3843 2091 1929 1012 306 159 BstAPI GCAn_nnn'nTGC Cuts at: 794 5416 6036 794 Size: 4622 620 4098 Fragments arranged by size: 4622 4098 620 BstBI TT'CG_AA Cuts at: 2179 8144 2179 Size: 5965 3375 BstEII G'GTnAC_C Cuts at: 1184 4175 1184 Size: 2991 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2665 4187 5258 5816 5860 6376 2665 Size: 1522 1071 558 44 516 5629 Fragments arranged by size: 5629 1522 1071 558 516 44 BstYI r'GATC_y Cuts at: 1972 4623 6477 6670 7037 7422 7439 8891 8977 Size: 2651 1854 193 367 385 17 1452 86 Cuts at: 8977 8989 9335 1972 Size: 12 346 1977 Fragments arranged by size: 2651 1977 1854 1452 385 367 346 193 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5034 5975 5034 Size: 941 8399 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 2701 3327 3856 4290 5373 Size: 552 939 576 121 626 529 434 1083 Cuts at: 5373 6243 6535 6597 513 Size: 870 292 62 3256 Fragments arranged by size: 3256 1083 939 870 626 576 552 529 434 292 121 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3174 487 Size: 683 56 1948 6653 Fragments arranged by size: 6653 1948 683 56 BtsI GCAGTG_nn' Cuts at: 5398 5414 5690 6856 7650 7670 5398 Size: 16 276 1166 794 20 7068 Fragments arranged by size: 7068 1166 794 276 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9305 DraI TTT'AAA Cuts at: 6644 6644 Size: 9340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3679 5818 8347 809 Size: 507 2363 2139 2529 1802 Fragments arranged by size: 2529 2363 2139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 4851 4898 Size: 54 1425 50 275 18 663 2358 47 Cuts at: 4898 4935 6787 7676 9305 8 Size: 37 1852 889 1629 43 Fragments arranged by size: 2358 1852 1629 1425 889 663 275 54 50 47 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 4898 6787 1537 Size: 275 3086 1889 4090 Fragments arranged by size: 4090 3086 1889 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2844 3492 4046 4485 4980 6224 7270 Size: 651 840 648 554 439 495 1244 1046 Cuts at: 7270 1353 Size: 3423 Fragments arranged by size: 3423 1244 1046 840 651 648 554 495 439 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2816 4199 7927 8301 8447 387 Size: 1064 138 1227 1383 3728 374 146 1280 Fragments arranged by size: 3728 1383 1280 1227 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2533 2824 2920 3046 3334 4012 2533 Size: 291 96 126 288 678 7861 Fragments arranged by size: 7861 678 291 288 126 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2318 2366 2435 2519 2543 2645 Size: 393 575 111 48 69 84 24 102 Cuts at: 2645 2708 2828 3038 3359 3473 3614 3776 3865 Size: 63 120 210 321 114 141 162 89 Cuts at: 3865 3908 4000 4129 4382 4934 5172 5556 5723 Size: 43 92 129 253 552 238 384 167 Cuts at: 5723 5895 6196 7403 8000 8787 9055 1239 Size: 172 301 1207 597 787 268 1524 Fragments arranged by size: 1524 1207 787 597 575 552 393 384 321 301 268 253 238 210 172 167 162 141 129 120 114 111 102 92 89 84 69 63 48 43 24 EcoNI CCTnn'n_nnAGG Cuts at: 2189 3008 3596 3710 5033 5608 2189 Size: 819 588 114 1323 575 5921 Fragments arranged by size: 5921 1323 819 588 575 114 EcoO109I rG'GnC_Cy Cuts at: 2329 2598 2752 2997 2998 3233 3479 4545 5140 Size: 269 154 245 1 235 246 1066 595 Cuts at: 5140 5516 5567 5820 5835 6253 6540 6593 2329 Size: 376 51 253 15 418 287 53 5076 Fragments arranged by size: 5076 1066 595 418 376 287 269 253 246 245 235 154 53 51 15 1 EcoRI G'AATT_C Cuts at: 4718 6649 7027 4718 Size: 1931 378 7031 Fragments arranged by size: 7031 1931 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 4564 4738 5078 6448 2428 Size: 2136 174 340 1370 5320 Fragments arranged by size: 5320 2136 1370 340 174 FseI GG_CCGG'CC Cuts at: 3776 3776 Size: 9340 FspI TGC'GCA Cuts at: 2483 5480 7846 2483 Size: 2997 2366 3977 Fragments arranged by size: 3977 2997 2366 HaeII r_GCGC'y Cuts at: 1678 3109 3415 4347 5647 8487 1678 Size: 1431 306 932 1300 2840 2531 Fragments arranged by size: 2840 2531 1431 1300 932 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3005 3037 3263 3295 3608 Size: 32 135 32 1433 32 226 32 313 Cuts at: 3608 3640 3719 3751 4001 4033 4252 4284 4862 Size: 32 79 32 250 32 219 32 578 Cuts at: 4862 4894 4916 4948 4949 4981 6194 6226 6551 Size: 32 22 32 1 32 1213 32 325 Cuts at: 6551 6583 7981 8013 8055 8087 1373 Size: 32 1398 32 42 32 2626 Fragments arranged by size: 2626 1433 1398 1213 578 325 313 250 226 219 135 79 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 22 1 HincII GTy'rAC Cuts at: 669 1657 1938 5488 6684 6930 669 Size: 988 281 3550 1196 246 3079 Fragments arranged by size: 3550 3079 1196 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9340 HpaI GTT'AAC Cuts at: 6930 6930 Size: 9340 KasI G'GCGC_C Cuts at: 5643 5643 Size: 9340 KpnI G_GTAC'C Cuts at: 5350 6665 5350 Size: 1315 8025 MfeI C'AATT_G Cuts at: 6939 6939 Size: 9340 MluI A'CGCG_T Cuts at: 9216 9216 Size: 9340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2317 3120 4902 8454 8638 1305 Size: 1012 803 1782 3552 184 2007 Fragments arranged by size: 3552 2007 1782 1012 803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 4853 10 Size: 54 1425 343 663 2358 4497 Fragments arranged by size: 4497 2358 1425 663 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3909 4203 5256 5858 6374 6419 7298 Size: 648 2743 294 1053 602 516 45 879 Cuts at: 7298 7657 7816 518 Size: 359 159 2042 Fragments arranged by size: 2743 2042 1053 879 648 602 516 359 294 159 45 MspA1I CmG'CkG Cuts at: 1606 2050 3051 3391 3535 3754 4292 4336 4486 Size: 444 1001 340 144 219 538 44 150 Cuts at: 4486 4532 4903 5892 6756 7433 8581 8826 1606 Size: 46 371 989 864 677 1148 245 2120 Fragments arranged by size: 2120 1148 1001 989 864 677 538 444 371 340 245 219 150 144 46 44 NaeI GCC'GGC Cuts at: 3774 6280 3774 Size: 2506 6834 NarI GG'CG_CC Cuts at: 5644 5644 Size: 9340 NcoI C'CATG_G Cuts at: 513 1065 2004 6597 513 Size: 552 939 4593 3256 Fragments arranged by size: 4593 3256 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9340 NgoMIV G'CCGG_C Cuts at: 3772 6278 3772 Size: 2506 6834 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9340 NotI GC'GGCC_GC Cuts at: 6787 6787 Size: 9340 NspI r_CATG'y Cuts at: 1132 1229 2731 4840 6036 6506 6698 8243 1132 Size: 97 1502 2109 1196 470 192 1545 2229 Fragments arranged by size: 2229 2109 1545 1502 1196 470 192 97 PasI CC'CwG_GG Cuts at: 1348 5674 6630 1348 Size: 4326 956 4058 Fragments arranged by size: 4326 4058 956 PciI A'CATG_T Cuts at: 8239 8239 Size: 9340 PflMI CCAn_nnn'nTGG Cuts at: 1108 3538 3862 5933 6571 1108 Size: 2430 324 2071 638 3877 Fragments arranged by size: 3877 2430 2071 638 324 PfoI T'CCnGG_A Cuts at: 3591 5822 5966 3591 Size: 2231 144 6965 Fragments arranged by size: 6965 2231 144 PmeI GTTT'AAAC Cuts at: 6644 6644 Size: 9340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5000 5032 5568 5600 7409 7441 8922 8954 5000 Size: 32 536 32 1809 32 1481 32 5386 Fragments arranged by size: 5386 1809 1481 536 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5516 5820 5835 6253 5516 Size: 304 15 418 8603 Fragments arranged by size: 8603 418 304 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9340 PsiI TTA'TAA Cuts at: 6910 6910 Size: 9340 PspOMI G'GGCC_C Cuts at: 2597 2752 2997 3721 6153 6319 6539 6593 2597 Size: 155 245 724 2432 166 220 54 5344 Fragments arranged by size: 5344 2432 724 245 220 166 155 54 PstI C_TGCA'G Cuts at: 830 2055 2106 2841 3009 3036 3072 3585 4287 Size: 1225 51 735 168 27 36 513 702 Cuts at: 4287 4416 6069 6692 830 Size: 129 1653 623 3478 Fragments arranged by size: 3478 1653 1225 735 702 623 513 168 129 51 36 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9340 PvuII CAG'CTG Cuts at: 2050 3535 3754 4336 4486 2050 Size: 1485 219 582 150 6904 Fragments arranged by size: 6904 1485 582 219 150 SacI G_AGCT'C Cuts at: 2535 2826 2922 3048 3336 4014 2535 Size: 291 96 126 288 678 7861 Fragments arranged by size: 7861 678 291 288 126 96 SacII CC_GC'GG Cuts at: 4293 4293 Size: 9340 SalI G'TCGA_C Cuts at: 1936 5486 6682 1936 Size: 3550 1196 4594 Fragments arranged by size: 4594 3550 1196 SanDI GG'GwC_CC Cuts at: 5516 5820 5835 5516 Size: 304 15 9021 Fragments arranged by size: 9021 304 15 SapI GCTCTTCn'nnn_ Cuts at: 4980 4980 Size: 9340 SbfI CC_TGCA'GG Cuts at: 3009 6692 3009 Size: 3683 5657 ScaI AGT'ACT Cuts at: 7588 9300 7588 Size: 1712 7628 SfcI C'TryA_G Cuts at: 826 1045 2051 2102 2837 3005 3032 3068 3581 Size: 219 1006 51 735 168 27 36 513 Cuts at: 3581 4283 4412 6065 6688 7823 8504 8695 826 Size: 702 129 1653 623 1135 681 191 1471 Fragments arranged by size: 1653 1471 1135 1006 735 702 681 623 513 219 191 168 129 51 36 27 SfoI GGC'GCC Cuts at: 5645 5645 Size: 9340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9340 SmaI CCC'GGG Cuts at: 1872 2612 2757 2948 3152 3347 3686 4253 5550 Size: 740 145 191 204 195 339 567 1297 Cuts at: 5550 5621 6546 6667 1872 Size: 71 925 121 4545 Fragments arranged by size: 4545 1297 925 740 567 339 204 195 191 145 121 71 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 2708 3916 4466 5198 5719 Size: 197 926 297 468 1208 550 732 521 Cuts at: 5719 7444 8345 8607 820 Size: 1725 901 262 1553 Fragments arranged by size: 1725 1553 1208 926 901 732 550 521 468 297 262 197 SnaBI TAC'GTA Cuts at: 493 7131 493 Size: 6638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9340 SphI G_CATG'C Cuts at: 1132 4840 6698 1132 Size: 3708 1858 3774 Fragments arranged by size: 3774 3708 1858 SspI AAT'ATT Cuts at: 5 52 7264 5 Size: 47 7212 2081 Fragments arranged by size: 7212 2081 47 StuI AGG'CCT Cuts at: 1517 3587 4355 1517 Size: 2070 768 6502 Fragments arranged by size: 6502 2070 768 StyI C'CwwG_G Cuts at: 513 1065 2004 5559 6597 6779 513 Size: 552 939 3555 1038 182 3074 Fragments arranged by size: 3555 3074 1038 939 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2788 4224 5254 5303 6276 7367 7384 Size: 1255 1208 1436 1030 49 973 1091 17 Cuts at: 7384 7537 7722 8167 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1436 1255 1208 1091 1030 973 445 185 153 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 2541 5202 Size: 274 80 33 51 1443 417 147 2661 Cuts at: 5202 7586 9298 96 Size: 2384 1712 138 Fragments arranged by size: 2661 2384 1712 1443 417 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4439 4471 4709 4741 9300 9332 4439 Size: 32 238 32 4559 32 4447 Fragments arranged by size: 4559 4447 238 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9340 XbaI T'CTAG_A Cuts at: 6676 6676 Size: 9340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3703 4854 3703 Size: 1151 8189 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9340 XmaI C'CCGG_G Cuts at: 1870 2610 2755 2946 3150 3345 3684 4251 5548 Size: 740 145 191 204 195 339 567 1297 Cuts at: 5548 5619 6544 6665 1870 Size: 71 925 121 4545 Fragments arranged by size: 4545 1297 925 740 567 339 204 195 191 145 121 71 XmnI GAAnn'nnTTC Cuts at: 3566 4034 4718 7469 3566 Size: 468 684 2751 5437 Fragments arranged by size: 5437 2751 684 468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3279 6111 7148 276 Size: 53 83 186 936 1745 2832 1037 2468 Fragments arranged by size: 2832 2468 1745 1037 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI