Restriction map for
pFN21AA0802
(Linear) (Six-base) MAPSORT of: KIAA0802.seq Check: 8784 from: 1 to: 4635
KIAA0802.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 839 1584 3490 3575 4635
Size: 839 745 1906 85 1060
Fragments arranged by size:
1906 1060 839 745 85
AatII G_ACGT'C
Cuts at: 0 1276 4108 4635
Size: 1276 2832 527
Fragments arranged by size:
2832 1276 527
AccI GT'mk_AC
Cuts at: 0 3482 4635
Size: 3482 1153
Acc65I G'GTAC_C
Cuts at: 0 3341 4635
Size: 3341 1294
AclI AA'CG_TT
Cuts at: 0 2798 4635
Size: 2798 1837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1033 1354 1860 2929 3890 4635
Size: 640 393 321 506 1069 961 745
Fragments arranged by size:
1069 961 745 640 506 393 321
AfeI AGC'GCT
Cuts at: 0 1102 4635
Size: 1102 3533
AflII C'TTAA_G
Cuts at: 0 235 4635
Size: 235 4400
AhdI GACnn_n'nnGTC
Cuts at: 0 1728 4635
Size: 1728 2907
AleI CACnn'nnGTG
Cuts at: 0 2198 3251 4414 4635
Size: 2198 1053 1163 221
Fragments arranged by size:
2198 1163 1053 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1922 2435 4635
Size: 1922 513 2200
Fragments arranged by size:
2200 1922 513
AlwNI CAG_nnn'CTG
Cuts at: 0 1533 2331 2869 3411 4635
Size: 1533 798 538 542 1224
Fragments arranged by size:
1533 1224 798 542 538
ApaI G_GGCC'C
Cuts at: 0 596 751 996 1720 4152 4318 4538 4592
Size: 596 155 245 724 2432 166 220 54
Cuts at: 4592 4635
Size: 43
Fragments arranged by size:
2432 724 596 245 220 166 155 54
43
ApaLI G'TGCA_C
Cuts at: 0 2563 4180 4635
Size: 2563 1617 455
Fragments arranged by size:
2563 1617 455
ApoI r'AATT_y
Cuts at: 0 2713 4635
Size: 2713 1922
AseI AT'TA_AT
Cuts at: 0 4193 4635
Size: 4193 442
AvaI C'yCGr_G
Cuts at: 0 380 605 750 941 1145 1340 1679 1762
Size: 380 225 145 191 204 195 339 83
Cuts at: 1762 1772 2146 2246 3001 3543 3614 4343 4539
Size: 10 374 100 755 542 71 729 196
Cuts at: 4539 4635
Size: 96
Fragments arranged by size:
755 729 542 380 374 339 225 204
196 195 191 145 100 96 83 71
10
BamHI G'GATC_C
Cuts at: 0 4472 4635
Size: 4472 163
BanI G'GyrC_C
Cuts at: 0 437 979 3341 3638 4333 4635
Size: 437 542 2362 297 695 302
Fragments arranged by size:
2362 695 542 437 302 297
BanII G_rGCy'C
Cuts at: 0 530 596 751 821 917 996 1043 1331
Size: 530 66 155 70 96 79 47 288
Cuts at: 1331 1526 1720 2009 3544 4152 4177 4301 4318
Size: 195 194 289 1535 608 25 124 17
Cuts at: 4318 4538 4592 4635
Size: 220 54 43
Fragments arranged by size:
1535 608 530 289 288 220 195 194
155 124 96 79 70 66 54 47
43 25 17
BbeI G_GCGC'C
Cuts at: 0 3642 4635
Size: 3642 993
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 893 1276 2497 2894 4635
Size: 100 793 383 1221 397 1741
Fragments arranged by size:
1741 1221 793 397 383 100
BbvCI CC'TCA_GC
Cuts at: 0 266 4635
Size: 266 4369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 71 1150 1184 4635
Size: 37 34 1079 34 3451
Fragments arranged by size:
3451 1079 37 34 34
BclI T'GATC_A
Cuts at: 0 2781 4635
Size: 2781 1854
BglI GCCn_nnn'nGGC
Cuts at: 0 954 1487 2347 4635
Size: 954 533 860 2288
Fragments arranged by size:
2288 954 860 533
BlpI GC'TnA_GC
Cuts at: 0 916 1042 3580 4290 4635
Size: 916 126 2538 710 345
Fragments arranged by size:
2538 916 710 345 126
Bme1580I G_kGCm'C
Cuts at: 0 596 751 996 1137 1720 2397 2567 3305
Size: 596 155 245 141 583 677 170 738
Cuts at: 3305 3636 4152 4184 4318 4411 4538 4592 4635
Size: 331 516 32 134 93 127 54 43
Fragments arranged by size:
738 677 596 583 516 331 245 170
155 141 134 127 93 54 43 32
BmgBI CAC'GTC
Cuts at: 0 2445 3423 4635
Size: 2445 978 1212
Fragments arranged by size:
2445 1212 978
BmrI ACTGGGnnnn_n'
Cuts at: 0 1133 4635
Size: 1133 3502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1266 2154 4635
Size: 1266 888 2481
Fragments arranged by size:
2481 1266 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 313 361 430 514 538 703 823
Size: 202 111 48 69 84 24 165 120
Cuts at: 823 1468 1609 1771 1903 1995 2124 2377 3167
Size: 645 141 162 132 92 129 253 790
Cuts at: 3167 3551 3718 4191 4635
Size: 384 167 473 444
Fragments arranged by size:
790 645 473 444 384 253 202 167
165 162 141 132 129 120 111 92
84 69 48 24
Bpu10I CC'TnA_GC
Cuts at: 0 266 4635
Size: 266 4369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1932 2446 3178 3735 4635
Size: 688 1244 514 732 557 900
Fragments arranged by size:
1244 900 732 688 557 514
BsaI GGTCTCn'nnnn_
Cuts at: 0 2125 4289 4549 4635
Size: 2125 2164 260 86
Fragments arranged by size:
2164 2125 260 86
BsaHI Gr'CG_yC
Cuts at: 0 1273 3639 4105 4635
Size: 1273 2366 466 530
Fragments arranged by size:
2366 1273 530 466
BsaWI w'CCGG_w
Cuts at: 0 863 2009 4635
Size: 863 1146 2626
Fragments arranged by size:
2626 1146 863
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 311 341 1000 1030 1246 1276 1996 2026
Size: 311 30 659 30 216 30 720 30
Cuts at: 2026 4635
Size: 2609
Fragments arranged by size:
2609 720 659 311 216 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 658 1021 1210 1270 1510 1906 1909 2107
Size: 658 363 189 60 240 396 3 198
Cuts at: 2107 2119 2511 3220 3324 4525 4530 4635
Size: 12 392 709 104 1201 5 105
Fragments arranged by size:
1201 709 658 396 392 363 240 198
189 105 104 60 12 5 3
BseYI C'CCAG_C
Cuts at: 0 598 769 1211 1382 1526 1533 1713 1857
Size: 598 171 442 171 144 7 180 144
Cuts at: 1857 3221 3928 4311 4566 4635
Size: 1364 707 383 255 69
Fragments arranged by size:
1364 707 598 442 383 255 180 171
171 144 144 69 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1804 2272 2882 3673 4027 4264 4407 4635
Size: 1804 468 610 791 354 237 143 228
Fragments arranged by size:
1804 791 610 468 354 237 228 143
BsiEI CG_ry'CG
Cuts at: 0 2670 2896 4635
Size: 2670 226 1739
Fragments arranged by size:
2670 1739 226
BsiHKAI G_wGCw'C
Cuts at: 0 530 821 917 1043 1058 1331 1901 2009
Size: 530 291 96 126 15 273 570 108
Cuts at: 2009 2234 2462 2567 2929 3395 4184 4635
Size: 225 228 105 362 466 789 451
Fragments arranged by size:
789 570 530 466 451 362 291 273
228 225 126 108 105 96 15
BsmI GAATG_Cn'
Cuts at: 0 773 3759 4635
Size: 773 2986 876
Fragments arranged by size:
2986 876 773
BsmBI CGTCTCn'nnnn_
Cuts at: 0 139 3959 4635
Size: 139 3820 676
Fragments arranged by size:
3820 676 139
Bsp1286I G_dGCh'C
Cuts at: 0 530 596 751 821 917 996 1043 1058
Size: 530 66 155 70 96 79 47 15
Cuts at: 1058 1137 1331 1526 1720 1901 2009 2234 2397
Size: 79 194 195 194 181 108 225 163
Cuts at: 2397 2462 2567 2929 3305 3395 3544 3636 4152
Size: 65 105 362 376 90 149 92 516
Cuts at: 4152 4177 4184 4301 4318 4411 4538 4592 4635
Size: 25 7 117 17 93 127 54 43
Fragments arranged by size:
530 516 376 362 225 195 194 194
181 163 155 149 127 117 108 105
96 93 92 90 79 79 70 66
65 54 47 43 25 17 15 7
BspEI T'CCGG_A
Cuts at: 0 863 4635
Size: 863 3772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 839 1584 1879 2615 3490 3575 4635
Size: 839 745 295 736 875 85 1060
Fragments arranged by size:
1060 875 839 745 736 295 85
BsrBI CCG'CTC
Cuts at: 0 741 1289 1395 1500 1785 2700 4154 4635
Size: 741 548 106 105 285 915 1454 481
Fragments arranged by size:
1454 915 741 548 481 285 106 105
BsrFI r'CCGG_y
Cuts at: 0 896 1767 2891 4273 4635
Size: 896 871 1124 1382 362
Fragments arranged by size:
1382 1124 896 871 362
BsrGI T'GTAC_A
Cuts at: 0 389 4635
Size: 389 4246
BssHII G'CGCG_C
Cuts at: 0 255 4635
Size: 255 4380
BssSI C'ACGA_G
Cuts at: 0 910 1069 1375 3304 4635
Size: 910 159 306 1929 1331
Fragments arranged by size:
1929 1331 910 306 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3411 4031 4635
Size: 3411 620 604
Fragments arranged by size:
3411 620 604
BstBI TT'CG_AA
Cuts at: 0 174 4635
Size: 174 4461
BstEII G'GTnAC_C
Cuts at: 0 2170 4635
Size: 2170 2465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 2182 3253 3811 3855 4371 4635
Size: 660 1522 1071 558 44 516 264
Fragments arranged by size:
1522 1071 660 558 516 264 44
BstYI r'GATC_y
Cuts at: 0 2618 4472 4635
Size: 2618 1854 163
Fragments arranged by size:
2618 1854 163
Bsu36I CC'TnA_GG
Cuts at: 0 3029 3970 4635
Size: 3029 941 665
Fragments arranged by size:
3029 941 665
BtgI C'CryG_G
Cuts at: 0 575 696 1322 1851 2285 3368 4238 4530
Size: 575 121 626 529 434 1083 870 292
Cuts at: 4530 4592 4635
Size: 62 43
Fragments arranged by size:
1083 870 626 575 529 434 292 121
62 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1169 4635
Size: 1169 3466
BtsI GCAGTG_nn'
Cuts at: 0 3393 3409 3685 4635
Size: 3393 16 276 950
Fragments arranged by size:
3393 950 276 16
DrdI GACnn_nn'nnGTC
Cuts at: 0 1674 3813 4635
Size: 1674 2139 822
Fragments arranged by size:
2139 1674 822
EaeI y'GGCC_r
Cuts at: 0 488 2846 2893 2930 4635
Size: 488 2358 47 37 1705
Fragments arranged by size:
2358 1705 488 47 37
EagI C'GGCC_G
Cuts at: 0 2893 4635
Size: 2893 1742
EarI CTCTTCn'nnn_
Cuts at: 0 839 1487 2041 2480 2975 4219 4635
Size: 839 648 554 439 495 1244 416
Fragments arranged by size:
1244 839 648 554 495 439 416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 811 2194 4635
Size: 811 1383 2441
Fragments arranged by size:
2441 1383 811
EcoICRI GAG'CTC
Cuts at: 0 528 819 915 1041 1329 2007 4635
Size: 528 291 96 126 288 678 2628
Fragments arranged by size:
2628 678 528 291 288 126 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 313 361 430 514 538 640 703
Size: 202 111 48 69 84 24 102 63
Cuts at: 703 823 1033 1354 1468 1609 1771 1860 1903
Size: 120 210 321 114 141 162 89 43
Cuts at: 1903 1995 2124 2377 2929 3167 3551 3718 3890
Size: 92 129 253 552 238 384 167 172
Cuts at: 3890 4191 4635
Size: 301 444
Fragments arranged by size:
552 444 384 321 301 253 238 210
202 172 167 162 141 129 120 114
111 102 92 89 84 69 63 48
43 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 1003 1591 1705 3028 3603 4635
Size: 184 819 588 114 1323 575 1032
Fragments arranged by size:
1323 1032 819 588 575 184 114
EcoO109I rG'GnC_Cy
Cuts at: 0 324 593 747 992 993 1228 1474 2540
Size: 324 269 154 245 1 235 246 1066
Cuts at: 2540 3135 3511 3562 3815 3830 4248 4535 4588
Size: 595 376 51 253 15 418 287 53
Cuts at: 4588 4635
Size: 47
Fragments arranged by size:
1066 595 418 376 324 287 269 253
246 245 235 154 53 51 47 15
1
EcoRI G'AATT_C
Cuts at: 0 2713 4635
Size: 2713 1922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 423 2559 2733 3073 4443 4635
Size: 423 2136 174 340 1370 192
Fragments arranged by size:
2136 1370 423 340 192 174
FseI GG_CCGG'CC
Cuts at: 0 1771 4635
Size: 1771 2864
FspI TGC'GCA
Cuts at: 0 478 3475 4635
Size: 478 2997 1160
Fragments arranged by size:
2997 1160 478
HaeII r_GCGC'y
Cuts at: 0 1104 1410 2342 3642 4635
Size: 1104 306 932 1300 993
Fragments arranged by size:
1300 1104 993 932 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1258 1290 1603 1635 1714 1746
Size: 1000 32 226 32 313 32 79 32
Cuts at: 1746 1996 2028 2247 2279 2857 2889 2911 2943
Size: 250 32 219 32 578 32 22 32
Cuts at: 2943 2944 2976 4189 4221 4546 4578 4635
Size: 1 32 1213 32 325 32 57
Fragments arranged by size:
1213 1000 578 325 313 250 226 219
79 57 32 32 32 32 32 32
32 32 32 32 32 22 1
HincII GTy'rAC
Cuts at: 0 3483 4635
Size: 3483 1152
KasI G'GCGC_C
Cuts at: 0 3638 4635
Size: 3638 997
KpnI G_GTAC'C
Cuts at: 0 3345 4635
Size: 3345 1290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1115 2897 4635
Size: 312 803 1782 1738
Fragments arranged by size:
1782 1738 803 312
MscI TGG'CCA
Cuts at: 0 490 2848 4635
Size: 490 2358 1787
Fragments arranged by size:
2358 1787 490
MslI CAynn'nnrTG
Cuts at: 0 1904 2198 3251 3853 4369 4414 4635
Size: 1904 294 1053 602 516 45 221
Fragments arranged by size:
1904 1053 602 516 294 221 45
MspA1I CmG'CkG
Cuts at: 0 45 1046 1386 1530 1749 2287 2331 2481
Size: 45 1001 340 144 219 538 44 150
Cuts at: 2481 2527 2898 3887 4635
Size: 46 371 989 748
Fragments arranged by size:
1001 989 748 538 371 340 219 150
144 46 45 44
NaeI GCC'GGC
Cuts at: 0 1769 4275 4635
Size: 1769 2506 360
Fragments arranged by size:
2506 1769 360
NarI GG'CG_CC
Cuts at: 0 3639 4635
Size: 3639 996
NcoI C'CATG_G
Cuts at: 0 4592 4635
Size: 4592 43
NgoMIV G'CCGG_C
Cuts at: 0 1767 4273 4635
Size: 1767 2506 362
Fragments arranged by size:
2506 1767 362
NspI r_CATG'y
Cuts at: 0 726 2835 4031 4501 4635
Size: 726 2109 1196 470 134
Fragments arranged by size:
2109 1196 726 470 134
PasI CC'CwG_GG
Cuts at: 0 3669 4625 4635
Size: 3669 956 10
Fragments arranged by size:
3669 956 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 1533 1857 3928 4566 4635
Size: 1533 324 2071 638 69
Fragments arranged by size:
2071 1533 638 324 69
PfoI T'CCnGG_A
Cuts at: 0 1586 3817 3961 4635
Size: 1586 2231 144 674
Fragments arranged by size:
2231 1586 674 144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2995 3027 3563 3595 4635
Size: 2995 32 536 32 1040
Fragments arranged by size:
2995 1040 536 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3511 3815 3830 4248 4635
Size: 3511 304 15 418 387
Fragments arranged by size:
3511 418 387 304 15
PspOMI G'GGCC_C
Cuts at: 0 592 747 992 1716 4148 4314 4534 4588
Size: 592 155 245 724 2432 166 220 54
Cuts at: 4588 4635
Size: 47
Fragments arranged by size:
2432 724 592 245 220 166 155 54
47
PstI C_TGCA'G
Cuts at: 0 50 101 836 1004 1031 1067 1580 2282
Size: 50 51 735 168 27 36 513 702
Cuts at: 2282 2411 4064 4635
Size: 129 1653 571
Fragments arranged by size:
1653 735 702 571 513 168 129 51
50 36 27
PvuII CAG'CTG
Cuts at: 0 45 1530 1749 2331 2481 4635
Size: 45 1485 219 582 150 2154
Fragments arranged by size:
2154 1485 582 219 150 45
SacI G_AGCT'C
Cuts at: 0 530 821 917 1043 1331 2009 4635
Size: 530 291 96 126 288 678 2626
Fragments arranged by size:
2626 678 530 291 288 126 96
SacII CC_GC'GG
Cuts at: 0 2288 4635
Size: 2288 2347
SalI G'TCGA_C
Cuts at: 0 3481 4635
Size: 3481 1154
SanDI GG'GwC_CC
Cuts at: 0 3511 3815 3830 4635
Size: 3511 304 15 805
Fragments arranged by size:
3511 805 304 15
SapI GCTCTTCn'nnn_
Cuts at: 0 2975 4635
Size: 2975 1660
SbfI CC_TGCA'GG
Cuts at: 0 1004 4635
Size: 1004 3631
SfcI C'TryA_G
Cuts at: 0 46 97 832 1000 1027 1063 1576 2278
Size: 46 51 735 168 27 36 513 702
Cuts at: 2278 2407 4060 4635
Size: 129 1653 575
Fragments arranged by size:
1653 735 702 575 513 168 129 51
46 36 27
SfoI GGC'GCC
Cuts at: 0 3640 4635
Size: 3640 995
SmaI CCC'GGG
Cuts at: 0 607 752 943 1147 1342 1681 2248 3545
Size: 607 145 191 204 195 339 567 1297
Cuts at: 3545 3616 4541 4635
Size: 71 925 94
Fragments arranged by size:
1297 925 607 567 339 204 195 191
145 94 71
SmlI C'TyrA_G
Cuts at: 0 235 703 1911 2461 3193 3714 4635
Size: 235 468 1208 550 732 521 921
Fragments arranged by size:
1208 921 732 550 521 468 235
SphI G_CATG'C
Cuts at: 0 2835 4635
Size: 2835 1800
StuI AGG'CCT
Cuts at: 0 1582 2350 4635
Size: 1582 768 2285
Fragments arranged by size:
2285 1582 768
StyI C'CwwG_G
Cuts at: 0 3554 4592 4635
Size: 3554 1038 43
Fragments arranged by size:
3554 1038 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 2219 3249 3298 4271 4635
Size: 783 1436 1030 49 973 364
Fragments arranged by size:
1436 1030 973 783 364 49
TatI w'GTAC_w
Cuts at: 0 389 536 3197 4635
Size: 389 147 2661 1438
Fragments arranged by size:
2661 1438 389 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2434 2466 2704 2736 4635
Size: 2434 32 238 32 1899
Fragments arranged by size:
2434 1899 238 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1698 2849 4635
Size: 1698 1151 1786
Fragments arranged by size:
1786 1698 1151
XmaI C'CCGG_G
Cuts at: 0 605 750 941 1145 1340 1679 2246 3543
Size: 605 145 191 204 195 339 567 1297
Cuts at: 3543 3614 4539 4635
Size: 71 925 96
Fragments arranged by size:
1297 925 605 567 339 204 195 191
145 96 71
XmnI GAAnn'nnTTC
Cuts at: 0 1561 2029 2713 4635
Size: 1561 468 684 1922
Fragments arranged by size:
1922 1561 684 468
ZraI GAC'GTC
Cuts at: 0 1274 4106 4635
Size: 1274 2832 529
Fragments arranged by size:
2832 1274 529
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI SbfI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflIII AgeI AloI AscI AsiSI AvrII BaeI BciVI
BfrBI BglII BmtI BsaAI BsaBI BsiWI BspHI BsrDI
BstZ17I ClaI CspCI DraI DraIII EcoRV FspAI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI