(Circular) (Six-base) MAPSORT of: pFN21AA0774.seq Check: 2734 from: 1 to: 8842 pFN21AA0774 8842 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2827 3278 4079 5422 2827 Size: 451 801 1343 6247 Fragments arranged by size: 6247 1343 801 451 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6652 278 Size: 53 83 186 936 5116 2468 Fragments arranged by size: 5116 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6185 1937 Size: 4248 4594 Acc65I G'GTAC_C Cuts at: 6163 6163 Size: 8842 AclI AA'CG_TT Cuts at: 1563 6969 7342 1563 Size: 5406 373 3063 Fragments arranged by size: 5406 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 4121 4144 4395 4461 4733 5057 5111 Size: 629 1860 23 251 66 272 324 54 Cuts at: 5111 5780 6905 8289 8557 1632 Size: 669 1125 1384 268 1917 Fragments arranged by size: 1917 1860 1384 1125 669 629 324 272 268 251 66 54 23 AflII C'TTAA_G Cuts at: 820 1017 2122 4331 820 Size: 197 1105 2209 5331 Fragments arranged by size: 5331 2209 1105 197 AflIII A'CryG_T Cuts at: 1203 2192 2827 3780 4052 7741 8718 1203 Size: 989 635 953 272 3689 977 1327 Fragments arranged by size: 3689 1327 989 977 953 635 272 AgeI A'CCGG_T Cuts at: 7673 7673 Size: 8842 AhdI GACnn_n'nnGTC Cuts at: 7571 7571 Size: 8842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4974 1184 Size: 3790 5052 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5261 5293 5261 Size: 32 8810 AlwNI CAG_nnn'CTG Cuts at: 2879 3997 5332 5404 5650 6585 8157 2879 Size: 1118 1335 72 246 935 1572 3564 Fragments arranged by size: 3564 1572 1335 1118 935 246 72 ApaI G_GGCC'C Cuts at: 6108 6108 Size: 8842 ApaLI G'TGCA_C Cuts at: 3765 4647 6899 8055 3765 Size: 882 2252 1156 4552 Fragments arranged by size: 4552 2252 1156 882 ApoI r'AATT_y Cuts at: 1493 2224 2264 2493 2887 3273 4127 5584 6151 Size: 731 40 229 394 386 854 1457 567 Cuts at: 6151 6377 6529 8638 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 854 731 567 394 386 229 226 152 40 AscI GG'CGCG_CC Cuts at: 5372 5372 Size: 8842 AseI AT'TA_AT Cuts at: 160 1032 7396 160 Size: 872 6364 1606 Fragments arranged by size: 6364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8842 AvaI C'yCGr_G Cuts at: 1870 1943 2640 4195 6100 6167 1870 Size: 73 697 1555 1905 67 4545 Fragments arranged by size: 4545 1905 1555 697 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4546 4579 4546 Size: 33 8809 BamHI G'GATC_C Cuts at: 3073 3822 6172 6539 3073 Size: 749 2350 367 5376 Fragments arranged by size: 5376 2350 749 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3481 6163 7618 618 Size: 325 217 627 1694 2682 1455 1842 Fragments arranged by size: 2682 1842 1694 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3233 3492 4607 5325 5665 6108 6161 1391 Size: 1842 259 1115 718 340 443 53 4072 Fragments arranged by size: 4072 1842 1115 718 443 340 259 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2693 3829 4150 4401 5751 5895 928 Size: 971 794 1136 321 251 1350 144 3875 Fragments arranged by size: 3875 1350 1136 971 794 321 251 144 BbvCI CC'TCA_GC Cuts at: 3601 3601 Size: 8842 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7033 7067 1903 Size: 34 5096 34 3678 Fragments arranged by size: 5096 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4547 4934 6734 7944 4547 Size: 387 1800 1210 5445 Fragments arranged by size: 5445 1800 1210 387 BclI T'GATC_A Cuts at: 1548 1548 Size: 8842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4580 5009 7453 Size: 107 122 71 631 605 2908 429 2444 Cuts at: 7453 136 Size: 1525 Fragments arranged by size: 2908 2444 1525 631 605 429 122 107 71 BglII A'GATC_T Cuts at: 5440 8837 5440 Size: 3397 5445 BlpI GC'TnA_GC Cuts at: 5013 5405 6259 5013 Size: 392 854 7596 Fragments arranged by size: 7596 854 392 Bme1580I G_kGCm'C Cuts at: 1790 2827 3769 4651 5164 6108 6903 8059 1790 Size: 1037 942 882 513 944 795 1156 2573 Fragments arranged by size: 2573 1156 1037 944 942 882 795 513 BmgBI CAC'GTC Cuts at: 1318 3783 4562 1318 Size: 2465 779 5598 Fragments arranged by size: 5598 2465 779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 3681 3810 Size: 416 53 83 399 24 622 1642 129 Cuts at: 3810 4627 6048 6573 7531 442 Size: 817 1421 525 958 1753 Fragments arranged by size: 1753 1642 1421 958 817 622 525 416 399 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2185 2622 5664 2185 Size: 437 3042 5363 Fragments arranged by size: 5363 3042 437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4591 4609 4752 4762 5288 5309 5354 5729 Size: 3352 18 143 10 526 21 45 375 Cuts at: 5729 5942 7502 1239 Size: 213 1560 2579 Fragments arranged by size: 3352 2579 1560 526 375 213 143 45 21 18 10 Bpu10I CC'TnA_GC Cuts at: 3486 3601 3486 Size: 115 8727 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5704 5837 6967 7832 8130 5704 Size: 133 1130 865 298 6416 Fragments arranged by size: 6416 1130 865 298 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2801 4544 7505 882 Size: 617 1302 1743 2961 2219 Fragments arranged by size: 2961 2219 1743 1302 617 BsaAI yAC'GTr Cuts at: 493 1204 6633 493 Size: 711 5429 2702 Fragments arranged by size: 5429 2702 711 BsaBI GATnn'nnATC Cuts at: 8836 8836 Size: 8842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4011 6649 7031 7666 Size: 53 83 186 936 2478 2638 382 635 Cuts at: 7666 275 Size: 1451 Fragments arranged by size: 2638 2478 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4166 4599 5412 7275 7673 7947 8094 8505 4166 Size: 433 813 1863 398 274 147 411 4503 Fragments arranged by size: 4503 1863 813 433 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5261 5291 6077 6107 1178 Size: 30 165 30 3858 30 786 30 3913 Fragments arranged by size: 3913 3858 786 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2161 2566 3195 3410 3920 4266 4606 5276 Size: 975 405 629 215 510 346 340 670 Cuts at: 5276 5351 5399 5714 6085 6088 6100 1186 Size: 75 48 315 371 3 12 3928 Fragments arranged by size: 3928 975 670 629 510 405 371 346 340 315 215 75 48 12 3 BseYI C'CCAG_C Cuts at: 4338 5068 5299 8045 8541 4338 Size: 730 231 2746 496 4639 Fragments arranged by size: 4639 2746 730 496 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2863 3306 1163 Size: 573 1127 443 6699 Fragments arranged by size: 6699 1127 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4756 6292 7053 8081 1540 Size: 275 186 2755 1536 761 1028 2301 Fragments arranged by size: 2755 2301 1536 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2798 2853 3769 4607 4651 5325 5391 6903 Size: 428 55 916 838 44 674 66 1512 Cuts at: 6903 6988 8059 2370 Size: 85 1071 3153 Fragments arranged by size: 3153 1512 1071 916 838 674 428 85 66 55 44 BsmI GAATG_Cn' Cuts at: 2146 2557 3583 6353 6446 2146 Size: 411 1026 2770 93 4542 Fragments arranged by size: 4542 2770 1026 411 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4568 4618 4568 Size: 50 8792 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2370 2798 2827 2853 3233 3492 3769 Size: 399 580 428 29 26 380 259 277 Cuts at: 3769 4607 4651 5164 5325 5391 5665 6108 6161 Size: 838 44 513 161 66 274 443 53 Cuts at: 6161 6903 6988 8059 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 838 742 580 513 443 428 399 380 277 274 259 161 85 66 53 44 29 26 BspEI T'CCGG_A Cuts at: 4166 4599 8505 4166 Size: 433 3906 4503 Fragments arranged by size: 4503 3906 433 BspHI T'CATG_A Cuts at: 2176 5554 6731 8461 2176 Size: 3378 1177 1730 2557 Fragments arranged by size: 3378 2557 1730 1177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2827 3278 4079 4149 4891 5422 5755 6197 Size: 1983 451 801 70 742 531 333 442 Cuts at: 6197 844 Size: 3489 Fragments arranged by size: 3489 1983 801 742 531 451 442 333 70 BsrBI CCG'CTC Cuts at: 1394 1823 3513 5017 5044 5341 6729 1394 Size: 429 1690 1504 27 297 1388 3507 Fragments arranged by size: 3507 1690 1504 1388 429 297 27 BsrDI GCAATG_nn' Cuts at: 65 1248 2690 4362 5606 7337 7511 65 Size: 1183 1442 1672 1244 1731 174 1396 Fragments arranged by size: 1731 1672 1442 1396 1244 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4665 4751 7486 7673 1700 Size: 110 2855 86 2735 187 2869 Fragments arranged by size: 2869 2855 2735 187 110 86 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8842 BssHII G'CGCG_C Cuts at: 1925 5372 1925 Size: 3447 5395 BssSI C'ACGA_G Cuts at: 5795 6902 7914 5795 Size: 1107 1012 6723 Fragments arranged by size: 6723 1107 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5026 794 Size: 4232 4610 BstBI TT'CG_AA Cuts at: 5673 7646 5673 Size: 1973 6869 BstEII G'GTnAC_C Cuts at: 1184 3538 4094 5393 1184 Size: 2354 556 1299 4633 Fragments arranged by size: 4633 2354 1299 556 BstXI CCAn_nnnn'nTGG Cuts at: 3146 5068 3146 Size: 1922 6920 BstYI r'GATC_y Cuts at: 1972 2965 3073 3822 5440 5862 6172 6539 6924 Size: 993 108 749 1618 422 310 367 385 Cuts at: 6924 6941 8393 8479 8491 8837 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1618 1452 993 749 422 385 367 346 310 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3241 3241 Size: 8842 BtgI C'CryG_G Cuts at: 513 1065 2004 5124 6088 513 Size: 552 939 3120 964 3267 Fragments arranged by size: 3267 3120 964 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8103 Fragments arranged by size: 8103 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3421 6358 7152 7172 2851 Size: 570 2937 794 20 4521 Fragments arranged by size: 4521 2937 794 570 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3532 3567 529 Size: 35 2968 35 5804 Fragments arranged by size: 5804 2968 35 35 DraI TTT'AAA Cuts at: 4488 6146 4488 Size: 1658 7184 DraIII CAC_nnn'GTG Cuts at: 2374 3284 3332 2374 Size: 910 48 7884 Fragments arranged by size: 7884 910 48 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2705 5531 7849 809 Size: 507 1389 2826 2318 1802 Fragments arranged by size: 2826 2318 1802 1389 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3115 3508 4667 Size: 54 1425 50 275 18 1285 393 1159 Cuts at: 4667 4753 5127 6117 6289 7178 8807 8 Size: 86 374 990 172 889 1629 43 Fragments arranged by size: 1629 1425 1285 1159 990 889 393 374 275 172 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4753 6289 1537 Size: 275 2941 1536 4090 Fragments arranged by size: 4090 2941 1536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 3039 3151 3165 3170 4071 4257 5087 Size: 1005 681 112 14 5 901 186 830 Cuts at: 5087 5288 5670 5747 5987 6009 6772 1353 Size: 201 382 77 240 22 763 3423 Fragments arranged by size: 3423 1005 901 830 763 681 382 240 201 186 112 77 22 14 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2854 4971 5378 7429 7803 7949 Size: 1064 138 1265 2117 407 2051 374 146 Cuts at: 7949 387 Size: 1280 Fragments arranged by size: 2117 2051 1280 1265 1064 407 374 146 138 EcoICRI GAG'CTC Cuts at: 4605 5323 4605 Size: 718 8124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 4121 4144 4395 4461 4591 4609 Size: 393 629 1860 23 251 66 130 18 Cuts at: 4609 4733 4752 4762 5057 5111 5288 5309 5354 Size: 124 19 10 295 54 177 21 45 Cuts at: 5354 5729 5780 5942 6905 7502 8289 8557 1239 Size: 375 51 162 963 597 787 268 1524 Fragments arranged by size: 1860 1524 963 787 629 597 393 375 295 268 251 177 162 130 124 66 54 51 45 23 21 19 18 10 EcoNI CCTnn'n_nnAGG Cuts at: 3976 4001 5381 3976 Size: 25 1380 7437 Fragments arranged by size: 7437 1380 25 EcoO109I rG'GnC_Cy Cuts at: 3732 3994 4436 6043 6104 6105 3732 Size: 262 442 1607 61 1 6469 Fragments arranged by size: 6469 1607 442 262 61 1 EcoRI G'AATT_C Cuts at: 6151 6529 6151 Size: 378 8464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3853 6038 3853 Size: 2185 6657 FseI GG_CCGG'CC Cuts at: 4669 4755 4669 Size: 86 8756 FspI TGC'GCA Cuts at: 5079 7348 5079 Size: 2269 6573 HaeII r_GCGC'y Cuts at: 1678 4422 4817 7989 1678 Size: 2744 395 3172 2531 Fragments arranged by size: 3172 2744 2531 395 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2743 2755 2775 2840 Size: 32 135 32 1151 20 12 20 65 Cuts at: 2840 2872 3038 3070 3716 3748 4384 4416 5536 Size: 32 166 32 646 32 636 32 1120 Cuts at: 5536 5568 7483 7515 7557 7589 1373 Size: 32 1915 32 42 32 2626 Fragments arranged by size: 2626 1915 1151 1120 646 636 166 135 65 42 32 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2031 3187 5420 6186 6432 669 Size: 988 281 93 1156 2233 766 246 3079 Fragments arranged by size: 3079 2233 1156 988 766 281 246 93 HindIII A'AGCT_T Cuts at: 748 4494 4925 748 Size: 3746 431 4665 Fragments arranged by size: 4665 3746 431 HpaI GTT'AAC Cuts at: 6432 6432 Size: 8842 KpnI G_GTAC'C Cuts at: 6167 6167 Size: 8842 MfeI C'AATT_G Cuts at: 6441 6441 Size: 8842 MluI A'CGCG_T Cuts at: 8718 8718 Size: 8842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2417 3442 4328 5899 5919 7956 8140 Size: 834 278 1025 886 1571 20 2037 184 Cuts at: 8140 1305 Size: 2007 Fragments arranged by size: 2037 2007 1571 1025 886 834 278 184 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 3117 5129 10 Size: 54 1425 343 1285 2012 3723 Fragments arranged by size: 3723 2012 1425 1285 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 6800 7159 7318 518 Size: 648 2766 2868 359 159 2042 Fragments arranged by size: 2868 2766 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4838 5023 5068 5350 5401 5707 5935 6090 Size: 3232 185 45 282 51 306 228 155 Cuts at: 6090 6258 6935 8083 8328 1606 Size: 168 677 1148 245 2120 Fragments arranged by size: 3232 2120 1148 677 306 282 245 228 185 168 155 51 45 NaeI GCC'GGC Cuts at: 4667 4753 4667 Size: 86 8756 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7351 Fragments arranged by size: 7351 939 552 NdeI CA'TA_TG Cuts at: 387 4039 387 Size: 3652 5190 NgoMIV G'CCGG_C Cuts at: 4665 4751 4665 Size: 86 8756 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8842 NotI GC'GGCC_GC Cuts at: 6289 6289 Size: 8842 NspI r_CATG'y Cuts at: 1132 1229 2196 2507 2831 3953 4056 6200 7745 Size: 97 967 311 324 1122 103 2144 1545 Cuts at: 7745 1132 Size: 2229 Fragments arranged by size: 2229 2144 1545 1122 967 324 311 103 97 PasI CC'CwG_GG Cuts at: 1348 3191 3997 1348 Size: 1843 806 6193 Fragments arranged by size: 6193 1843 806 PciI A'CATG_T Cuts at: 2192 2827 4052 7741 2192 Size: 635 1225 3689 3293 Fragments arranged by size: 3689 3293 1225 635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2818 3997 5332 1108 Size: 1710 1179 1335 4618 Fragments arranged by size: 4618 1710 1335 1179 PfoI T'CCnGG_A Cuts at: 4549 5325 5958 4549 Size: 776 633 7433 Fragments arranged by size: 7433 776 633 PmeI GTTT'AAAC Cuts at: 6146 6146 Size: 8842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5261 5293 6911 6943 8424 8456 5261 Size: 32 1618 32 1481 32 5647 Fragments arranged by size: 5647 1618 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3732 4436 6043 3732 Size: 704 1607 6531 Fragments arranged by size: 6531 1607 704 PshAI GACnn'nnGTC Cuts at: 1613 5935 1613 Size: 4322 4520 PsiI TTA'TAA Cuts at: 6412 6412 Size: 8842 PspOMI G'GGCC_C Cuts at: 6104 6104 Size: 8842 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3653 3685 3653 Size: 32 8810 PstI C_TGCA'G Cuts at: 830 2400 2593 2838 4146 5349 5382 5514 6194 Size: 1570 193 245 1308 1203 33 132 680 Cuts at: 6194 830 Size: 3478 Fragments arranged by size: 3478 1570 1308 1203 680 245 193 132 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8842 PvuII CAG'CTG Cuts at: 5023 5068 5350 5401 5707 5935 5023 Size: 45 282 51 306 228 7930 Fragments arranged by size: 7930 306 282 228 51 45 SacI G_AGCT'C Cuts at: 4607 5325 4607 Size: 718 8124 SacII CC_GC'GG Cuts at: 6091 6091 Size: 8842 SalI G'TCGA_C Cuts at: 1936 6184 1936 Size: 4248 4594 SanDI GG'GwC_CC Cuts at: 3732 6043 3732 Size: 2311 6531 SapI GCTCTTCn'nnn_ Cuts at: 2358 6009 2358 Size: 3651 5191 SbfI CC_TGCA'GG Cuts at: 5382 5514 6194 5382 Size: 132 680 8030 Fragments arranged by size: 8030 680 132 ScaI AGT'ACT Cuts at: 7090 8802 7090 Size: 1712 7130 SexAI A'CCwGG_T Cuts at: 3328 5616 3328 Size: 2288 6554 SfcI C'TryA_G Cuts at: 826 1045 2396 2583 2589 2834 4142 4462 5345 Size: 219 1351 187 6 245 1308 320 883 Cuts at: 5345 5378 5510 6190 7325 8006 8197 826 Size: 33 132 680 1135 681 191 1471 Fragments arranged by size: 1471 1351 1308 1135 883 681 680 320 245 219 191 187 132 33 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8842 SmaI CCC'GGG Cuts at: 1872 2642 4197 6169 1872 Size: 770 1555 1972 4545 Fragments arranged by size: 4545 1972 1555 770 SmlI C'TyrA_G Cuts at: 820 1017 1943 2122 4331 5683 5816 6946 7847 Size: 197 926 179 2209 1352 133 1130 901 Cuts at: 7847 8109 820 Size: 262 1553 Fragments arranged by size: 2209 1553 1352 1130 926 901 262 197 179 133 SnaBI TAC'GTA Cuts at: 493 6633 493 Size: 6140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8842 SphI G_CATG'C Cuts at: 1132 6200 1132 Size: 5068 3774 SspI AAT'ATT Cuts at: 5 52 3628 6766 5 Size: 47 3576 3138 2081 Fragments arranged by size: 3576 3138 2081 47 StuI AGG'CCT Cuts at: 1517 5681 1517 Size: 4164 4678 StyI C'CwwG_G Cuts at: 513 1065 2004 2511 3060 3145 3381 3738 3849 Size: 552 939 507 549 85 236 357 111 Cuts at: 3849 4151 4743 6281 513 Size: 302 592 1538 3074 Fragments arranged by size: 3074 1538 939 592 552 549 507 357 302 236 111 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3290 3660 3700 4305 4340 4443 4646 Size: 1255 1710 370 40 605 35 103 203 Cuts at: 4646 4756 6151 6869 6886 7039 7224 7669 325 Size: 110 1395 718 17 153 185 445 1498 Fragments arranged by size: 1710 1498 1395 1255 718 605 445 370 203 185 153 110 103 40 35 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2830 7088 8800 Size: 274 80 33 51 1443 853 4258 1712 Cuts at: 8800 96 Size: 138 Fragments arranged by size: 4258 1712 1443 853 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2739 2771 4161 4193 8802 8834 2739 Size: 32 1390 32 4609 32 2747 Fragments arranged by size: 4609 2747 1390 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2424 1316 Size: 1108 7734 XbaI T'CTAG_A Cuts at: 2464 3571 6178 2464 Size: 1107 2607 5128 Fragments arranged by size: 5128 2607 1107 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 8842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8842 XmaI C'CCGG_G Cuts at: 1870 2640 4195 6167 1870 Size: 770 1555 1972 4545 Fragments arranged by size: 4545 1972 1555 770 XmnI GAAnn'nnTTC Cuts at: 6971 6971 Size: 8842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6650 276 Size: 53 83 186 936 5116 2468 Fragments arranged by size: 5116 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SfiI SfoI SrfI SwaI