Restriction map for
pFN21AA0773
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# NO CUT ENZYMES ( 137 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AvrII BbeI
BbrPI BcgI BclI BglII BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1024 GACGTC GACGT^C
AccI 996 GTCTAC GT^[AC][GT]AC
AfaI 669 GTAC GT^AC
AflII 211 CTTAAG C^TTAAG
AflIII 653 ACGTGT A^C[GA][CT]GT
AhdI 958 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 589 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 958 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 401 TGCGCA TGC^GCA
BalI 248 TGGCCA TGG^CCA
BamHI 736 GGATCC G^GATCC
BanI 924 GGCACC G^G[CT][GA]CC
BfaI 460 CTAG C^TAG
BfrI 211 CTTAAG C^TTAAG
BglI 721 GCCTCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 652 TACGTG [CT]AC^GT[GA]
BsaBI 67 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 848 GAGAC GTCTC(1/5)
BsrBI 1001 CCGCTC CCGCTC(-3/-3)
BsrDI 717 CATTGC GCAATG(2/0)
BsrI 857 ACTGG ACTGG(1/-1)
BssSI 977 CACGAG CACGAG(-5/-1)
Eam1105I 958 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 946 GCGGG CCCGC(4/6)
FspI 401 TGCGCA TGC^GCA
HgaI 467 GCGTC GACGC(5/10)
HindIII 208 AAGCTT A^AGCTT
MaeI 460 CTAG C^TAG
MamI 67 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MseI 212 TTAA T^TAA
MspA1I 368 CAGCTG C[AC]G^C[GT]G
PvuII 368 CAGCTG CAG^CTG
RsaI 669 GTAC GT^AC
SfcI 573 CTACAG C^T[GA][CT]AG
Tru9I 212 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 1001 CCGC CCGC(-3/-1)
946 GCGG
AcyI 466 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
AocI 928 CCTGAGG CC^T[ACGT]AGG
1034 CCTCAGG
ApaI 242 GGGCCC GGGCC^C
894 GGGCCC
BbvCI 429 CCTCAGC CCTCAGC(-5/-2)
901 GCTGAGG
BciVI 408 GGATAC GTATCC(6/5)
811 GGATAC
BpmI 823 CTGGAG CTGGAG(16/14)
961 CTGGAG
BsaHI 466 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
BsgI 103 CTGCAC GTGCAG(16/14)
1042 GTGCAG
BstXI 305 CCACAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
497 CCACACTCATGG
BstYI 269 GGATCT [GA]^GATC[CT]
736 GGATCC
Bsu36I 928 CCTGAGG CC^T[ACGT]AGG
1034 CCTCAGG
CfoI 402 GCGC GCG^C
875 GCGC
EarI 912 GAAGAG CTCTTC(1/4)
937 GAAGAG
Eco81I 928 CCTGAGG CC^T[ACGT]AGG
1034 CCTCAGG
HhaI 402 GCGC GCG^C
875 GCGC
Hin1I 466 GGCGTC G[GA]^CG[CT]C
1024 GACGTC
HinP1I 402 GCGC G^CGC
875 GCGC
HphI 36 GGTGA GGTGA(8/7)
526 GGTGA
Ksp632I 912 GAAGAG CTCTTC(1/4)
937 GAAGAG
MflI 269 GGATCT [GA]^GATC[CT]
736 GGATCC
PpuMI 789 GGGACCT [GA]G^G[AT]CC[CT]
956 GGGACCT
SmlI 211 CTTAAG C^T[CT][GA]AG
620 CTCAAG
XhoII 269 GGATCT [GA]^GATC[CT]
736 GGATCC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AluI AlwI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I