(Circular) (Six-base) MAPSORT of: pFN21AA0773.seq Check: 4376 from: 1 to: 5785 pFN21AA0773 5785 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3033 3595 278 Size: 53 83 186 936 1497 562 2468 Fragments arranged by size: 2468 1497 936 562 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 3128 1937 Size: 1065 126 4594 Fragments arranged by size: 4594 1065 126 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3084 3912 4285 1563 Size: 1521 828 373 3063 Fragments arranged by size: 3063 1521 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2796 3848 5232 5500 1632 Size: 455 709 1052 1384 268 1917 Fragments arranged by size: 1917 1384 1052 709 455 268 AflII C'TTAA_G Cuts at: 820 1017 2216 820 Size: 197 1199 4389 Fragments arranged by size: 4389 1199 197 AflIII A'CryG_T Cuts at: 1203 2658 4684 5661 1203 Size: 1455 2026 977 1327 Fragments arranged by size: 2026 1455 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 2968 4514 2968 Size: 1546 4239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2599 3528 5100 2599 Size: 929 1572 3284 Fragments arranged by size: 3284 1572 929 ApaI G_GGCC'C Cuts at: 2251 2903 2251 Size: 652 5133 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 3094 3320 3472 5581 1493 Size: 1601 226 152 2109 1697 Fragments arranged by size: 2109 1697 1601 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 2741 3115 3482 2741 Size: 374 367 5044 Fragments arranged by size: 5044 374 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2929 3106 4561 618 Size: 325 217 627 1142 177 1455 1842 Fragments arranged by size: 1842 1455 1142 627 325 217 177 BanII G_rGCy'C Cuts at: 1391 2251 2869 2903 3104 1391 Size: 860 618 34 201 4072 Fragments arranged by size: 4072 860 618 201 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2372 2511 2994 928 Size: 971 473 139 483 3719 Fragments arranged by size: 3719 971 483 473 139 BbvCI CC'TCA_GC Cuts at: 2435 2907 2435 Size: 472 5313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2407 2810 3677 4887 2407 Size: 403 867 1210 3305 Fragments arranged by size: 3305 1210 867 403 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 4396 136 Size: 107 122 71 631 605 1060 1664 1525 Fragments arranged by size: 1664 1525 1060 631 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2251 2903 3846 5002 1790 Size: 461 652 943 1156 2573 Fragments arranged by size: 2573 1156 943 652 461 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2872 3516 4474 Size: 416 53 83 399 24 1455 644 958 Cuts at: 4474 442 Size: 1753 Fragments arranged by size: 1753 1455 958 644 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2849 2987 4445 1239 Size: 1610 138 1458 2579 Fragments arranged by size: 2579 1610 1458 138 Bpu10I CC'TnA_GC Cuts at: 2435 2907 2435 Size: 472 5313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2610 3910 4775 5073 2610 Size: 1300 865 298 3322 Fragments arranged by size: 3322 1300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4448 882 Size: 617 2949 2219 Fragments arranged by size: 2949 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2659 3576 493 Size: 711 1455 917 2702 Fragments arranged by size: 2702 1455 917 711 BsaBI GATnn'nnATC Cuts at: 2076 5779 2076 Size: 3703 2082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 3030 3592 3974 Size: 53 83 186 936 939 558 562 382 Cuts at: 3974 4609 275 Size: 635 1451 Fragments arranged by size: 1451 939 936 635 562 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2924 2927 2951 3077 1186 Size: 1738 3 24 126 3894 Fragments arranged by size: 3894 1738 126 24 3 BseYI C'CCAG_C Cuts at: 2358 2777 2845 4988 5484 2358 Size: 419 68 2143 496 2659 Fragments arranged by size: 2659 2143 496 419 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 3068 1163 Size: 573 357 975 3880 Fragments arranged by size: 3880 975 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2251 2869 2903 3104 3846 3931 5002 Size: 399 461 618 34 201 742 85 1071 Cuts at: 5002 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 618 461 399 201 85 34 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3140 844 Size: 2296 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3008 3672 1394 Size: 429 1185 664 3507 Fragments arranged by size: 3507 1185 664 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 4280 4454 65 Size: 1183 1473 1559 174 1396 Fragments arranged by size: 1559 1473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 2982 3845 4857 2982 Size: 863 1012 3910 Fragments arranged by size: 3910 1012 863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2509 2317 Size: 192 5593 BstYI r'GATC_y Cuts at: 1972 2274 2741 3115 3482 3867 3884 5336 5422 Size: 302 467 374 367 385 17 1452 86 Cuts at: 5422 5434 5780 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 467 385 374 367 346 302 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2934 3040 2934 Size: 106 5679 BtgI C'CryG_G Cuts at: 513 1065 2004 2184 2316 2523 2634 513 Size: 552 939 180 132 207 111 3664 Fragments arranged by size: 3664 939 552 207 180 132 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2454 487 Size: 683 56 1228 3818 Fragments arranged by size: 3818 1228 683 56 BtsI GCAGTG_nn' Cuts at: 2055 2105 2889 3301 4095 4115 2055 Size: 50 784 412 794 20 3725 Fragments arranged by size: 3725 794 784 412 50 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 2253 2733 Size: 54 1425 50 275 18 299 124 480 Cuts at: 2733 3232 4121 5750 8 Size: 499 889 1629 43 Fragments arranged by size: 1629 1425 889 499 480 299 275 124 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2912 2937 3715 1353 Size: 1559 25 778 3423 Fragments arranged by size: 3423 1559 778 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2796 2849 2987 3848 4445 5232 Size: 393 455 709 53 138 861 597 787 Cuts at: 5232 5500 1239 Size: 268 1524 Fragments arranged by size: 1524 861 787 709 597 455 393 268 138 53 EcoO109I rG'GnC_Cy Cuts at: 2062 2247 2248 2795 2899 2900 2962 2062 Size: 185 1 547 104 1 62 4885 Fragments arranged by size: 4885 547 185 104 62 1 1 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 2408 4291 2408 Size: 1883 3902 HaeII r_GCGC'y Cuts at: 1678 4932 1678 Size: 3254 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2593 2625 2954 Size: 32 135 32 839 32 150 32 329 Cuts at: 2954 2986 3020 3052 4426 4458 4500 4532 1373 Size: 32 34 32 1374 32 42 32 2626 Fragments arranged by size: 2626 1374 839 329 150 135 42 34 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3129 3375 669 Size: 988 281 1191 246 3079 Fragments arranged by size: 3079 1191 988 281 246 HindIII A'AGCT_T Cuts at: 748 2213 748 Size: 1465 4320 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2454 4899 5083 1305 Size: 1149 2445 184 2007 Fragments arranged by size: 2445 2007 1149 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 3540 Fragments arranged by size: 3540 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2394 2507 2543 3743 4102 4261 Size: 648 1149 79 113 36 1200 359 159 Cuts at: 4261 518 Size: 2042 Fragments arranged by size: 2042 1200 1149 648 359 159 113 79 36 MspA1I CmG'CkG Cuts at: 1606 2375 3201 3878 5026 5271 1606 Size: 769 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 769 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2184 2316 2523 2634 513 Size: 552 939 180 132 207 111 3664 Fragments arranged by size: 3664 939 552 207 180 132 111 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 2665 1348 Size: 1317 4468 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PfoI T'CCnGG_A Cuts at: 2021 2021 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2795 2962 2795 Size: 167 5618 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2247 2899 2247 Size: 652 5133 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2375 2375 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2578 3133 4268 4949 5140 826 Size: 219 1533 555 1135 681 191 1471 Fragments arranged by size: 1533 1471 1135 681 555 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2625 3889 4790 5052 820 Size: 197 926 273 409 1264 901 262 1553 Fragments arranged by size: 1553 1264 926 901 409 273 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2316 2523 2634 3224 513 Size: 552 939 180 132 207 111 590 3074 Fragments arranged by size: 3074 939 590 552 207 180 132 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2570 2647 3812 3829 3982 4167 4612 Size: 1255 990 77 1165 17 153 185 445 Cuts at: 4612 325 Size: 1498 Fragments arranged by size: 1498 1255 1165 990 445 185 153 77 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 4031 5743 Size: 274 80 33 51 1443 696 1358 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1712 1443 1358 696 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3031 3593 276 Size: 53 83 186 936 1497 562 2468 Fragments arranged by size: 2468 1497 936 562 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI