(Circular) (Six-base) MAPSORT of: pFN21AA0766.seq Check: 6682 from: 1 to: 6526 pFN21AA0766 6526 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2166 4336 278 Size: 53 83 186 936 630 2170 2468 Fragments arranged by size: 2468 2170 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2367 3869 1937 Size: 103 327 1502 4594 Fragments arranged by size: 4594 1502 327 103 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 3518 4589 5973 6241 1632 Size: 672 1214 1071 1384 268 1917 Fragments arranged by size: 1917 1384 1214 1071 672 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 5425 6402 1203 Size: 4222 977 1327 Fragments arranged by size: 4222 1327 977 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 5255 5255 Size: 6526 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3606 1184 Size: 2422 4104 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3425 3457 3425 Size: 32 6494 AlwNI CAG_nnn'CTG Cuts at: 4269 5841 4269 Size: 1572 4954 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 6526 ApaLI G'TGCA_C Cuts at: 4583 5739 4583 Size: 1156 5370 ApoI r'AATT_y Cuts at: 1493 2954 3262 3413 3835 4061 4213 6322 1493 Size: 1461 308 151 422 226 152 2109 1697 Fragments arranged by size: 2109 1697 1461 422 308 226 152 151 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2309 2405 3851 1870 Size: 73 120 246 96 1446 4545 Fragments arranged by size: 4545 1446 246 120 96 73 BamHI G'GATC_C Cuts at: 2463 3856 4223 2463 Size: 1393 367 4766 Fragments arranged by size: 4766 1393 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 3847 5302 618 Size: 325 217 627 382 1678 1455 1842 Fragments arranged by size: 1842 1678 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 2116 2316 2870 3783 3845 1391 Size: 725 200 554 913 62 4072 Fragments arranged by size: 4072 913 725 554 200 62 BbeI G_GCGC'C Cuts at: 2173 2173 Size: 6526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5555 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4717 4751 1903 Size: 34 2780 34 3678 Fragments arranged by size: 3678 2780 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4418 5628 4418 Size: 1210 5316 BclI T'GATC_A Cuts at: 1548 1548 Size: 6526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5137 136 Size: 107 122 71 631 605 3465 1525 Fragments arranged by size: 3465 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2623 6521 2623 Size: 3898 2628 BlpI GC'TnA_GC Cuts at: 2184 2711 3943 2184 Size: 527 1232 4767 Fragments arranged by size: 4767 1232 527 Bme1580I G_kGCm'C Cuts at: 1790 2116 4587 5743 1790 Size: 326 2471 1156 2573 Fragments arranged by size: 2573 2471 1156 326 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4257 5215 442 Size: 416 53 83 399 24 2840 958 1753 Fragments arranged by size: 2840 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2313 2313 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 5186 1239 Size: 1188 2759 2579 Fragments arranged by size: 2759 2579 1188 Bpu10I CC'TnA_GC Cuts at: 2468 3029 2468 Size: 561 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4651 5516 5814 4651 Size: 865 298 5363 Fragments arranged by size: 5363 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5189 882 Size: 617 3690 2219 Fragments arranged by size: 3690 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4317 493 Size: 711 3113 2702 Fragments arranged by size: 3113 2702 711 BsaBI GATnn'nnATC Cuts at: 2886 3381 3738 6520 2886 Size: 495 357 2782 2892 Fragments arranged by size: 2892 2782 495 357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2163 2170 4333 Size: 53 83 186 936 482 148 7 2163 Cuts at: 4333 4715 5350 275 Size: 382 635 1451 Fragments arranged by size: 2163 1451 936 635 482 382 186 148 83 53 7 BsaWI w'CCGG_w Cuts at: 2098 4959 5357 5631 5778 6189 2098 Size: 2861 398 274 147 411 2435 Fragments arranged by size: 2861 2435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6301 Fragments arranged by size: 6301 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6526 BseYI C'CCAG_C Cuts at: 2073 2346 5729 6225 2073 Size: 273 3383 496 2374 Fragments arranged by size: 3383 2374 496 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2322 1163 Size: 573 586 5367 Fragments arranged by size: 5367 586 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3976 4737 5765 1540 Size: 275 186 1975 761 1028 2301 Fragments arranged by size: 2301 1975 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2870 4587 4672 5743 2190 Size: 680 1717 85 1071 2973 Fragments arranged by size: 2973 1717 1071 680 85 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 BsmBI CGTCTCn'nnnn_ Cuts at: 2788 3407 2788 Size: 619 5907 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2190 2316 2870 3783 3845 4587 Size: 399 326 74 126 554 913 62 742 Cuts at: 4587 4672 5743 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 913 742 554 399 326 126 85 74 62 BspEI T'CCGG_A Cuts at: 2098 6189 2098 Size: 4091 2435 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3881 844 Size: 3037 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2203 4413 1394 Size: 429 380 2210 3507 Fragments arranged by size: 3507 2210 429 380 BsrDI GCAATG_nn' Cuts at: 65 1248 5021 5195 65 Size: 1183 3773 174 1396 Fragments arranged by size: 3773 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5170 5357 1700 Size: 110 3360 187 2869 Fragments arranged by size: 3360 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 2224 1925 Size: 299 6227 BssSI C'ACGA_G Cuts at: 2298 4586 5598 2298 Size: 2288 1012 3226 Fragments arranged by size: 3226 2288 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6526 BstBI TT'CG_AA Cuts at: 3249 5330 3249 Size: 2081 4445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6526 BstYI r'GATC_y Cuts at: 1972 2463 2623 3856 4223 4608 4625 6077 6163 Size: 491 160 1233 367 385 17 1452 86 Cuts at: 6163 6175 6521 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1233 491 385 367 346 160 86 17 12 BstZ17I GTA'TAC Cuts at: 2368 2368 Size: 6526 Bsu36I CC'TnA_GG Cuts at: 3437 3437 Size: 6526 BtgI C'CryG_G Cuts at: 513 1065 2265 2590 513 Size: 552 1200 325 4449 Fragments arranged by size: 4449 1200 552 325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 4892 Fragments arranged by size: 4892 895 683 56 BtsI GCAGTG_nn' Cuts at: 4042 4836 4856 4042 Size: 794 20 5712 Fragments arranged by size: 5712 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 2521 3048 3487 3830 2521 Size: 527 439 343 5217 Fragments arranged by size: 5217 527 439 343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5533 809 Size: 507 4217 1802 Fragments arranged by size: 4217 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3973 4862 6491 Size: 54 1425 50 275 18 2143 889 1629 Cuts at: 6491 8 Size: 43 Fragments arranged by size: 2143 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 4456 1353 Size: 927 2176 3423 Fragments arranged by size: 3423 2176 927 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2228 5113 5487 5633 387 Size: 1064 138 639 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 639 374 146 138 EcoICRI GAG'CTC Cuts at: 2868 2868 Size: 6526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2427 3518 4589 5186 5973 6241 Size: 393 672 123 1091 1071 597 787 268 Cuts at: 6241 1239 Size: 1524 Fragments arranged by size: 1524 1091 1071 787 672 597 393 268 123 EcoO109I rG'GnC_Cy Cuts at: 2112 2113 2600 3433 2112 Size: 1 487 833 5205 Fragments arranged by size: 5205 833 487 1 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 6526 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2642 2642 Size: 6526 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 2173 5673 1678 Size: 495 3500 2531 Fragments arranged by size: 3500 2531 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3385 3417 5167 5199 5241 Size: 32 135 32 1813 32 1750 32 42 Cuts at: 5241 5273 1373 Size: 32 2626 Fragments arranged by size: 2626 1813 1750 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 2434 3870 4116 669 Size: 988 281 103 393 1436 246 3079 Fragments arranged by size: 3079 1436 988 393 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KasI G'GCGC_C Cuts at: 2169 2169 Size: 6526 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 3022 4125 3022 Size: 1103 5423 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2589 2710 2801 5640 5824 1305 Size: 1284 121 91 2839 184 2007 Fragments arranged by size: 2839 2007 1284 184 121 91 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4704 Fragments arranged by size: 4704 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2381 2658 4484 4843 5002 518 Size: 648 843 372 277 1826 359 159 2042 Fragments arranged by size: 2042 1826 843 648 372 359 277 159 MspA1I CmG'CkG Cuts at: 1606 2592 3942 4619 5767 6012 1606 Size: 986 1350 677 1148 245 2120 Fragments arranged by size: 2120 1350 1148 986 677 245 NarI GG'CG_CC Cuts at: 2170 2170 Size: 6526 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5974 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 2414 3884 5429 1132 Size: 97 1185 1470 1545 2229 Fragments arranged by size: 2229 1545 1470 1185 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6526 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2820 1108 Size: 1269 443 4814 Fragments arranged by size: 4814 1269 443 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3433 3433 Size: 6526 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6526 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 6526 PstI C_TGCA'G Cuts at: 830 2700 3878 830 Size: 1870 1178 3478 Fragments arranged by size: 3478 1870 1178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 SacI G_AGCT'C Cuts at: 2870 2870 Size: 6526 SacII CC_GC'GG Cuts at: 2593 2593 Size: 6526 SalI G'TCGA_C Cuts at: 1936 2039 3868 1936 Size: 103 1829 4594 Fragments arranged by size: 4594 1829 103 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2696 3874 5009 5690 5881 826 Size: 219 1651 1178 1135 681 191 1471 Fragments arranged by size: 1651 1471 1178 1135 681 219 191 SfoI GGC'GCC Cuts at: 2171 2171 Size: 6526 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 3853 1872 Size: 1981 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4630 5531 5793 820 Size: 197 926 2687 901 262 1553 Fragments arranged by size: 2687 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 3057 3366 4450 5 Size: 47 3005 309 1084 2081 Fragments arranged by size: 3005 2081 1084 309 47 StuI AGG'CCT Cuts at: 1517 2307 1517 Size: 790 5736 StyI C'CwwG_G Cuts at: 513 1065 2538 3965 513 Size: 552 1473 1427 3074 Fragments arranged by size: 3074 1473 1427 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4553 4570 4723 4908 5353 325 Size: 1255 2973 17 153 185 445 1498 Fragments arranged by size: 2973 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3242 4772 6484 Size: 274 80 33 51 1443 1265 1530 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 1712 1530 1443 1265 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 6486 6518 2749 Size: 32 3705 32 2757 Fragments arranged by size: 3705 2757 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3012 1316 Size: 1696 4830 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3772 3772 Size: 6526 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6526 XmaI C'CCGG_G Cuts at: 1870 3851 1870 Size: 1981 4545 XmnI GAAnn'nnTTC Cuts at: 2625 3174 4655 2625 Size: 549 1481 4496 Fragments arranged by size: 4496 1481 549 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2164 4334 276 Size: 53 83 186 936 630 2170 2468 Fragments arranged by size: 2468 2170 936 630 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI