(Circular) (Six-base) MAPSORT of: pFN21AA0765.seq Check: 6426 from: 1 to: 7501 pFN21AA0765 7501 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5311 278 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4844 1937 Size: 2907 4594 Acc65I G'GTAC_C Cuts at: 4822 4822 Size: 7501 AclI AA'CG_TT Cuts at: 1563 2513 5628 6001 1563 Size: 950 3115 373 3063 Fragments arranged by size: 3115 3063 950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3559 3671 4254 5564 6948 7216 1632 Size: 1927 112 583 1310 1384 268 1917 Fragments arranged by size: 1927 1917 1384 1310 583 268 112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7304 AflIII A'CryG_T Cuts at: 1203 3337 3727 6400 7377 1203 Size: 2134 390 2673 977 1327 Fragments arranged by size: 2673 2134 1327 977 390 AgeI A'CCGG_T Cuts at: 6332 6332 Size: 7501 AhdI GACnn_n'nnGTC Cuts at: 6230 6230 Size: 7501 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4049 1184 Size: 2865 4636 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2464 2496 4293 4325 2464 Size: 32 1797 32 5640 Fragments arranged by size: 5640 1797 32 32 AlwNI CAG_nnn'CTG Cuts at: 4742 5244 6816 4742 Size: 502 1572 5427 Fragments arranged by size: 5427 1572 502 ApaI G_GGCC'C Cuts at: 4370 4370 Size: 7501 ApaLI G'TGCA_C Cuts at: 5558 6714 5558 Size: 1156 6345 ApoI r'AATT_y Cuts at: 1493 2803 4375 4810 5036 5188 7297 1493 Size: 1310 1572 435 226 152 2109 1697 Fragments arranged by size: 2109 1697 1572 1310 435 226 152 AseI AT'TA_AT Cuts at: 160 1032 6055 160 Size: 872 5023 1606 Fragments arranged by size: 5023 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 AvaI C'yCGr_G Cuts at: 1870 1943 4411 4722 4826 1870 Size: 73 2468 311 104 4545 Fragments arranged by size: 4545 2468 311 104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2355 2388 2355 Size: 33 7468 BamHI G'GATC_C Cuts at: 4831 5198 4831 Size: 367 7134 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 4822 6277 618 Size: 325 217 627 974 2061 1455 1842 Fragments arranged by size: 2061 1842 1455 974 627 325 217 BanII G_rGCy'C Cuts at: 1391 2510 2985 4370 4820 1391 Size: 1119 475 1385 450 4072 Fragments arranged by size: 4072 1385 1119 475 450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5692 5726 1903 Size: 34 3755 34 3678 Fragments arranged by size: 3755 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2383 5393 6603 2383 Size: 3010 1210 3281 Fragments arranged by size: 3281 3010 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7501 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3250 4709 6112 Size: 107 122 71 631 605 1578 1459 1403 Cuts at: 6112 136 Size: 1525 Fragments arranged by size: 1578 1525 1459 1403 631 605 122 107 71 BglII A'GATC_T Cuts at: 7496 7496 Size: 7501 BlpI GC'TnA_GC Cuts at: 4918 4918 Size: 7501 Bme1580I G_kGCm'C Cuts at: 1790 2676 3643 4370 4450 5562 6718 1790 Size: 886 967 727 80 1112 1156 2573 Fragments arranged by size: 2573 1156 1112 967 886 727 80 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2132 2391 2681 Size: 416 53 83 399 24 715 259 290 Cuts at: 2681 3240 3701 3987 4077 5232 6190 442 Size: 559 461 286 90 1155 958 1753 Fragments arranged by size: 1753 1155 958 715 559 461 416 399 290 286 259 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2625 2811 3180 4206 6161 1239 Size: 1386 186 369 1026 1955 2579 Fragments arranged by size: 2579 1955 1386 1026 369 186 Bpu10I CC'TnA_GC Cuts at: 2335 4399 2335 Size: 2064 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3048 5626 6491 6789 3048 Size: 2578 865 298 3760 Fragments arranged by size: 3760 2578 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 6164 882 Size: 617 537 4128 2219 Fragments arranged by size: 4128 2219 617 537 BsaAI yAC'GTr Cuts at: 493 1204 3800 5292 493 Size: 711 2596 1492 2702 Fragments arranged by size: 2702 2596 1492 711 BsaBI GATnn'nnATC Cuts at: 7495 7495 Size: 7501 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5308 5690 6325 275 Size: 53 83 186 936 3775 382 635 1451 Fragments arranged by size: 3775 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5934 6332 6606 6753 7164 5934 Size: 398 274 147 411 6271 Fragments arranged by size: 6271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2464 2494 2527 2557 2641 Size: 30 165 30 1061 30 33 30 84 Cuts at: 2641 2671 1178 Size: 30 6008 Fragments arranged by size: 6008 1061 165 84 33 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2469 2628 3207 4209 1186 Size: 1283 159 579 1002 4478 Fragments arranged by size: 4478 1283 1002 579 159 BseYI C'CCAG_C Cuts at: 4022 6704 7200 4022 Size: 2682 496 4323 Fragments arranged by size: 4323 2682 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4017 4032 4077 4107 4122 1163 Size: 573 2281 15 45 30 15 4542 Fragments arranged by size: 4542 2281 573 45 30 15 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4951 5712 6740 1540 Size: 275 186 2950 761 1028 2301 Fragments arranged by size: 2950 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2797 5562 5647 6718 2797 Size: 2765 85 1071 3580 Fragments arranged by size: 3580 2765 1071 85 BsmI GAATG_Cn' Cuts at: 2940 3102 3963 4083 5012 5105 2940 Size: 162 861 120 929 93 5336 Fragments arranged by size: 5336 929 861 162 120 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 2676 2797 2985 3643 4370 4450 Size: 399 720 166 121 188 658 727 80 Cuts at: 4450 4820 5562 5647 6718 1391 Size: 370 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 727 720 658 399 370 188 166 121 85 80 BspEI T'CCGG_A Cuts at: 7164 7164 Size: 7501 BspHI T'CATG_A Cuts at: 2425 5390 7120 2425 Size: 2965 1730 2806 Fragments arranged by size: 2965 2806 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2779 4856 844 Size: 1935 2077 3489 Fragments arranged by size: 3489 2077 1935 BsrBI CCG'CTC Cuts at: 1394 1823 5388 1394 Size: 429 3565 3507 Fragments arranged by size: 3565 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2651 2955 5996 6170 65 Size: 1183 1403 304 3041 174 1396 Fragments arranged by size: 3041 1403 1396 1183 304 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6145 6332 1700 Size: 110 4335 187 2869 Fragments arranged by size: 4335 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3724 96 Size: 3628 3873 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7501 BssSI C'ACGA_G Cuts at: 5561 6573 5561 Size: 1012 6489 BstAPI GCAn_nnn'nTGC Cuts at: 794 2935 4591 794 Size: 2141 1656 3704 Fragments arranged by size: 3704 2141 1656 BstBI TT'CG_AA Cuts at: 3556 6305 3556 Size: 2749 4752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7501 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4526 2011 Size: 2515 4986 BstYI r'GATC_y Cuts at: 1972 4831 5198 5583 5600 7052 7138 7150 7496 Size: 2859 367 385 17 1452 86 12 346 Cuts at: 7496 1972 Size: 1977 Fragments arranged by size: 2859 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 4704 513 Size: 552 939 48 2652 3310 Fragments arranged by size: 3310 2652 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2603 487 Size: 683 56 1377 5385 Fragments arranged by size: 5385 1377 683 56 BtsI GCAGTG_nn' Cuts at: 2714 2887 3003 5017 5811 5831 2714 Size: 173 116 2014 794 20 4384 Fragments arranged by size: 4384 2014 794 173 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7501 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4324 4359 529 Size: 35 3760 35 3671 Fragments arranged by size: 3760 3671 35 35 DraI TTT'AAA Cuts at: 3355 3781 4668 4805 3355 Size: 426 887 137 6051 Fragments arranged by size: 6051 887 426 137 DraIII CAC_nnn'GTG Cuts at: 2399 2399 Size: 7501 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6508 809 Size: 507 5192 1802 Fragments arranged by size: 5192 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4948 5837 7466 Size: 54 1425 50 275 18 3118 889 1629 Cuts at: 7466 8 Size: 43 Fragments arranged by size: 3118 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4948 1537 Size: 275 3136 4090 Fragments arranged by size: 4090 3136 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4107 5431 1353 Size: 2754 1324 3423 Fragments arranged by size: 3423 2754 1324 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2739 6088 6462 6608 387 Size: 1064 138 1150 3349 374 146 1280 Fragments arranged by size: 3349 1280 1150 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2625 2811 3180 3559 3671 4206 4254 Size: 393 993 186 369 379 112 535 48 Cuts at: 4254 5564 6161 6948 7216 1239 Size: 1310 597 787 268 1524 Fragments arranged by size: 1524 1310 993 787 597 535 393 379 369 268 186 112 48 EcoNI CCTnn'n_nnAGG Cuts at: 2334 3903 3933 4101 4221 4296 2334 Size: 1569 30 168 120 75 5539 Fragments arranged by size: 5539 1569 168 120 75 30 EcoO109I rG'GnC_Cy Cuts at: 3191 4239 4366 4367 3191 Size: 1048 127 1 6325 Fragments arranged by size: 6325 1048 127 1 EcoRI G'AATT_C Cuts at: 4810 5188 4810 Size: 378 7123 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4805 4805 Size: 7501 FspI TGC'GCA Cuts at: 2180 6007 2180 Size: 3827 3674 FspAI rTGC'GCAy Cuts at: 2180 2180 Size: 7501 HaeII r_GCGC'y Cuts at: 1678 6648 1678 Size: 4970 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 2857 2889 3153 Size: 32 135 32 1009 32 244 32 264 Cuts at: 3153 3185 3589 3608 3621 3640 6142 6174 6216 Size: 32 404 19 13 19 2502 32 42 Cuts at: 6216 6248 1373 Size: 32 2626 Fragments arranged by size: 2626 2502 1009 404 264 244 135 42 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2854 3622 4845 5091 669 Size: 988 281 916 768 1223 246 3079 Fragments arranged by size: 3079 1223 988 916 768 281 246 HindIII A'AGCT_T Cuts at: 748 3078 3843 748 Size: 2330 765 4406 Fragments arranged by size: 4406 2330 765 HpaI GTT'AAC Cuts at: 2854 5091 2854 Size: 2237 5264 KpnI G_GTAC'C Cuts at: 4826 4826 Size: 7501 MfeI C'AATT_G Cuts at: 2037 5100 2037 Size: 3063 4438 MluI A'CGCG_T Cuts at: 7377 7377 Size: 7501 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 4255 6615 6799 1305 Size: 1095 1855 2360 184 2007 Fragments arranged by size: 2360 2007 1855 1095 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5679 Fragments arranged by size: 5679 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4461 4524 5459 5818 5977 518 Size: 648 843 2452 63 935 359 159 2042 Fragments arranged by size: 2452 2042 935 843 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 4739 4917 5594 6742 6987 1606 Size: 3133 178 677 1148 245 2120 Fragments arranged by size: 3133 2120 1148 677 245 178 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 4704 513 Size: 552 939 48 2652 3310 Fragments arranged by size: 3310 2652 939 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 7501 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7501 NotI GC'GGCC_GC Cuts at: 4948 4948 Size: 7501 NspI r_CATG'y Cuts at: 1132 1229 2737 3341 3731 4121 4594 4859 6404 Size: 97 1508 604 390 390 473 265 1545 Cuts at: 6404 1132 Size: 2229 Fragments arranged by size: 2229 1545 1508 604 473 390 390 265 97 PasI CC'CwG_GG Cuts at: 1348 4451 1348 Size: 3103 4398 PciI A'CATG_T Cuts at: 3337 3727 6400 3337 Size: 390 2673 4438 Fragments arranged by size: 4438 2673 390 PflMI CCAn_nnn'nTGG Cuts at: 1108 4478 1108 Size: 3370 4131 PfoI T'CCnGG_A Cuts at: 3952 4222 3952 Size: 270 7231 PmeI GTTT'AAAC Cuts at: 4805 4805 Size: 7501 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2464 2496 5570 5602 7083 7115 2464 Size: 32 3074 32 1481 32 2850 Fragments arranged by size: 3074 2850 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3191 3191 Size: 7501 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7501 PsiI TTA'TAA Cuts at: 5071 5071 Size: 7501 PspOMI G'GGCC_C Cuts at: 4366 4366 Size: 7501 PstI C_TGCA'G Cuts at: 830 3570 3949 4853 830 Size: 2740 379 904 3478 Fragments arranged by size: 3478 2740 904 379 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7501 PvuII CAG'CTG Cuts at: 4739 4739 Size: 7501 SalI G'TCGA_C Cuts at: 1936 4843 1936 Size: 2907 4594 SapI GCTCTTCn'nnn_ Cuts at: 4107 4107 Size: 7501 SbfI CC_TGCA'GG Cuts at: 4853 4853 Size: 7501 ScaI AGT'ACT Cuts at: 5749 7461 5749 Size: 1712 5789 SfcI C'TryA_G Cuts at: 826 1045 3566 3945 4131 4767 4849 5984 6665 Size: 219 2521 379 186 636 82 1135 681 Cuts at: 6665 6856 826 Size: 191 1471 Fragments arranged by size: 2521 1471 1135 681 636 379 219 191 186 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7501 SmaI CCC'GGG Cuts at: 1872 4413 4828 1872 Size: 2541 415 4545 Fragments arranged by size: 4545 2541 415 SmlI C'TyrA_G Cuts at: 820 1017 1943 3063 5605 6506 6768 820 Size: 197 926 1120 2542 901 262 1553 Fragments arranged by size: 2542 1553 1120 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5292 493 Size: 4799 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7501 SphI G_CATG'C Cuts at: 1132 2737 4121 4859 1132 Size: 1605 1384 738 3774 Fragments arranged by size: 3774 1605 1384 738 SspI AAT'ATT Cuts at: 5 52 3655 5425 5 Size: 47 3603 1770 2081 Fragments arranged by size: 3603 2081 1770 47 StuI AGG'CCT Cuts at: 1517 4017 4258 4765 1517 Size: 2500 241 507 4253 Fragments arranged by size: 4253 2500 507 241 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 3904 4704 4940 513 Size: 552 939 48 1852 800 236 3074 Fragments arranged by size: 3074 1852 939 800 552 236 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 2410 2719 2737 4451 4582 5528 Size: 1255 534 296 309 18 1714 131 946 Cuts at: 5528 5545 5698 5883 6328 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1714 1498 1255 946 534 445 309 296 185 153 131 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3483 3724 5747 Size: 274 80 33 51 1443 1506 241 2023 Cuts at: 5747 7459 96 Size: 1712 138 Fragments arranged by size: 2023 1712 1506 1443 274 241 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2527 2559 3693 3725 7461 7493 2527 Size: 32 1134 32 3736 32 2535 Fragments arranged by size: 3736 2535 1134 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7501 XbaI T'CTAG_A Cuts at: 4837 4837 Size: 7501 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2072 2072 Size: 7501 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7501 XmaI C'CCGG_G Cuts at: 1870 4411 4826 1870 Size: 2541 415 4545 Fragments arranged by size: 4545 2541 415 XmnI GAAnn'nnTTC Cuts at: 5630 5630 Size: 7501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5309 276 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI