Restriction map for
pFN21AA0761
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI Asp700I AspEI
AspI AvaI AviII AvrII BbeI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI SfcI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1374 GGTACC G^GTACC
AflIII 642 ACGTGT A^C[GA][CT]GT
AlwNI 1641 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 993 AGCGCT AGC^GCT
ApaI 1212 GGGCCC GGGCC^C
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
Asp718I 1374 GGTACC G^GTACC
BalI 1494 TGGCCA TGG^CCA
BbrPI 1619 CACGTG CAC^GTG
BciVI 1401 GGATAC GTATCC(6/5)
BcnI 1326 CCGGG CC^[GC]GG
BsaAI 1619 CACGTG [CT]AC^GT[GA]
BsaWI 1632 TCCGGT [AT]^CCGG[AT]
BsmBI 639 GAGACG CGTCTC(1/5)
BsmFI 880 GTCCC GGGAC(10/14)
BsmI 25 GAATGC GAATGC(1/-1)
BspMI 1177 GCAGGT ACCTGC(4/8)
BssSI 1355 CACGAG CACGAG(-5/-1)
BstAPI 1177 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 520 TTCGAA TT^CGAA
BstXI 764 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 994 GCGC GCG^C
DraIII 461 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1256 GAAGAG CTCTTC(1/4)
Eco47III 993 AGCGCT AGC^GCT
EcoT22I 361 ATGCAT ATGCA^T
HaeII 993 AGCGCT [GA]GCGC^[CT]
HgaI 1345 GACGC GACGC(5/10)
HhaI 994 GCGC GCG^C
HinP1I 994 GCGC G^CGC
HindIII 328 AAGCTT A^AGCTT
KpnI 1374 GGTACC GGTAC^C
Ksp632I 1256 GAAGAG CTCTTC(1/4)
MluNI 1494 TGGCCA TGG^CCA
MscI 1494 TGGCCA TGG^CCA
MspA1I 1532 CAGCGG C[AC]G^C[GT]G
NciI 1326 CCGGG CC^[GC]GG
NdeI 614 CATATG CA^TATG
NheI 702 GCTAGC G^CTAGC
NsiI 361 ATGCAT ATGCA^T
NspI 83 ACATGC [GA]CATG^[CT]
NspV 520 TTCGAA TT^CGAA
PacI 475 TTAATTAA TTAAT^TAA
PmaCI 1619 CACGTG CAC^GTG
PmlI 1619 CACGTG CAC^GTG
PshBI 478 ATTAAT AT^TAAT
SacI 850 GAGCTC GAGCT^C
ScaI 1459 AGTACT AGT^ACT
SexAI 441 ACCAGGT A^CC[AT]GGT
SfiI 1204 GGCCAAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 520 TTCGAA TT^CGAA
SpeI 858 ACTAGT A^CTAGT
TaqI 521 TCGA T^CGA
TthHB8I 521 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 961 GAATTT [GA]^AATT[CT]
986 AAATTC
ApoI 961 GAATTT [GA]^AATT[CT]
986 AAATTC
AspHI 850 GAGCTC G[AT]GC[AT]^C
1321 GTGCTC
BamHI 540 GGATCC G^GATCC
1629 GGATCC
BanI 445 GGTGCC G^G[CT][GA]CC
1374 GGTACC
BfaI 703 CTAG C^TAG
859 CTAG
BglI 1205 GCCAAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1643 GCCCCTGTGGC
BsiHKAI 850 GAGCTC G[AT]GC[AT]^C
1321 GTGCTC
BstYI 540 GGATCC [GA]^GATC[CT]
1629 GGATCC
Cac8I 702 GCTAGC GC[ACGT]^[ACGT]GC
1564 GCAGGC
DpnII 541 GATC ^GATC
1630 GATC
DraII 1212 GGGCCCC [GA]G^G[ACGT]CC[CT]
1227 AGGCCCT
DsaI 1126 CCACGG C^C[GA][CT]GG
1367 CCACGG
EcoO109I 1212 GGGCCCC [GA]G^G[ACGT]CC[CT]
1227 AGGCCCT
FauI 1528 CCCGC CCCGC(4/6)
1597 CCCGC
HapII 1326 CCGG C^CGG
1633 CCGG
HincII 862 GTCAAC GT[CT]^[GA]AC
1282 GTTGAC
HindII 862 GTCAAC GT[CT]^[GA]AC
1282 GTTGAC
HpaII 1326 CCGG C^CGG
1633 CCGG
MaeI 703 CTAG C^TAG
859 CTAG
MaeIII 904 GTTAC ^GT[ACGT]AC
922 GTAAC
MboI 541 GATC ^GATC
1630 GATC
MflI 540 GGATCC [GA]^GATC[CT]
1629 GGATCC
MspI 1326 CCGG C^CGG
1633 CCGG
NdeII 541 GATC ^GATC
1630 GATC
Sau3AI 541 GATC ^GATC
1630 GATC
SmlI 1287 CTTGAG C^T[CT][GA]AG
1549 CTCAAG
TspRI 1010 CAGTG CA[GC]TG[ACGT][ACGT]^
1218 CACTG
XhoII 540 GGATCC [GA]^GATC[CT]
1629 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BbvI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MboII MnlI MseI
MslI MvaI MwoI NlaIII NlaIV
RcaI RsaI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I