(Circular) (Six-base) MAPSORT of: pFN21AA0761.seq Check: 728 from: 1 to: 6358 pFN21AA0761 6358 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3173 3173 Size: 6358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4168 278 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3701 1937 Size: 1764 4594 Acc65I G'GTAC_C Cuts at: 3379 3679 3379 Size: 300 6058 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2751 3342 3582 4421 5805 6073 1632 Size: 462 657 591 240 839 1384 268 1917 Fragments arranged by size: 1917 1384 839 657 591 462 268 240 AfeI AGC'GCT Cuts at: 3000 3000 Size: 6358 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 2647 5257 6234 1203 Size: 1444 2610 977 1327 Fragments arranged by size: 2610 1444 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2391 1184 Size: 1207 5151 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3057 3089 3057 Size: 32 6326 AlwNI CAG_nnn'CTG Cuts at: 3651 4101 5673 3651 Size: 450 1572 4336 Fragments arranged by size: 4336 1572 450 ApaI G_GGCC'C Cuts at: 3221 3221 Size: 6358 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 2966 2991 3667 3893 4045 6154 1493 Size: 1473 25 676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1473 676 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 2484 4912 160 Size: 872 1452 2428 1606 Fragments arranged by size: 2428 1606 1452 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 3683 1870 Size: 73 1740 4545 Fragments arranged by size: 4545 1740 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3363 3396 3363 Size: 33 6325 BamHI G'GATC_C Cuts at: 2545 3634 3688 4055 2545 Size: 1089 54 367 4848 Fragments arranged by size: 4848 1089 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 3379 3679 5134 618 Size: 325 217 627 663 929 300 1455 1842 Fragments arranged by size: 1842 1455 929 663 627 325 300 217 BanII G_rGCy'C Cuts at: 1391 2859 3221 3320 3677 1391 Size: 1468 362 99 357 4072 Fragments arranged by size: 4072 1468 362 357 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2271 2442 3148 928 Size: 971 372 171 706 4138 Fragments arranged by size: 4138 971 706 372 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4549 4583 1903 Size: 34 50 34 2528 34 3678 Fragments arranged by size: 3678 2528 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3400 4250 5460 3400 Size: 850 1210 4298 Fragments arranged by size: 4298 1210 850 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BfrBI ATG'CAT Cuts at: 2368 2368 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3216 3654 4969 Size: 107 122 71 631 605 1544 438 1315 Cuts at: 4969 136 Size: 1525 Fragments arranged by size: 1544 1525 1315 631 605 438 122 107 71 BglII A'GATC_T Cuts at: 6353 6353 Size: 6358 BlpI GC'TnA_GC Cuts at: 3775 3775 Size: 6358 Bme1580I G_kGCm'C Cuts at: 1790 3221 4419 5575 1790 Size: 1431 1198 1156 2573 Fragments arranged by size: 2573 1431 1198 1156 BmgBI CAC'GTC Cuts at: 1318 2648 1318 Size: 1330 5028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3378 4089 Size: 416 53 83 399 24 1553 408 711 Cuts at: 4089 5047 442 Size: 958 1753 Fragments arranged by size: 1753 1553 958 711 416 408 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2711 1056 Size: 1655 4703 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2145 3129 2145 Size: 984 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5018 1239 Size: 3779 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3313 3539 4483 5348 5646 3313 Size: 226 944 865 298 4025 Fragments arranged by size: 4025 944 865 298 226 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5021 882 Size: 617 3522 2219 Fragments arranged by size: 3522 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3626 4149 493 Size: 711 2422 523 2702 Fragments arranged by size: 2702 2422 711 523 BsaBI GATnn'nnATC Cuts at: 6352 6352 Size: 6358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4165 4547 5182 275 Size: 53 83 186 936 2632 382 635 1451 Fragments arranged by size: 2632 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3637 4791 5189 5463 5610 6021 3637 Size: 1154 398 274 147 411 3974 Fragments arranged by size: 3974 1154 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6133 Fragments arranged by size: 6133 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6358 BseYI C'CCAG_C Cuts at: 3549 5561 6057 3549 Size: 2012 496 3850 Fragments arranged by size: 3850 2012 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5785 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2859 3330 4419 4504 5575 2859 Size: 471 1089 85 1071 3642 Fragments arranged by size: 3642 1089 1071 471 85 BsmI GAATG_Cn' Cuts at: 2036 3869 3962 2036 Size: 1833 93 4432 Fragments arranged by size: 4432 1833 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2638 2638 Size: 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2859 3221 3320 3330 3677 4419 4504 Size: 399 1069 362 99 10 347 742 85 Cuts at: 4504 5575 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1069 742 399 362 347 99 85 10 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 2059 2497 2982 4247 5977 2059 Size: 438 485 1265 1730 2440 Fragments arranged by size: 2440 1730 1265 485 438 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 3713 844 Size: 2329 540 3489 Fragments arranged by size: 3489 2329 540 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4853 5027 65 Size: 1183 3605 174 1396 Fragments arranged by size: 3605 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5002 5189 1700 Size: 110 3192 187 2869 Fragments arranged by size: 3192 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 3360 4418 5430 3360 Size: 1058 1012 4288 Fragments arranged by size: 4288 1058 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3188 794 Size: 2394 3964 BstBI TT'CG_AA Cuts at: 2526 5162 2526 Size: 2636 3722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstXI CCAn_nnnn'nTGG Cuts at: 2776 2776 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2545 3634 3688 4055 4440 4457 5909 5995 Size: 573 1089 54 367 385 17 1452 86 Cuts at: 5995 6007 6353 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1089 573 385 367 346 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 3131 3372 513 Size: 552 2066 241 3499 Fragments arranged by size: 3499 2066 552 241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 3874 4668 4688 3874 Size: 794 20 5544 Fragments arranged by size: 5544 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3594 3629 529 Size: 35 3030 35 3258 Fragments arranged by size: 3258 3030 35 35 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DraIII CAC_nnn'GTG Cuts at: 2471 2471 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3128 3483 3499 Size: 54 1425 50 275 18 1298 355 16 Cuts at: 3499 3805 4694 6323 8 Size: 306 889 1629 43 Fragments arranged by size: 1629 1425 1298 889 355 306 275 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 4288 1353 Size: 1903 1032 3423 Fragments arranged by size: 3423 1903 1032 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4945 5319 5465 387 Size: 1064 138 3356 374 146 1280 Fragments arranged by size: 3356 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2857 2857 Size: 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2751 3342 3582 4421 5018 5805 Size: 393 462 657 591 240 839 597 787 Cuts at: 5805 6073 1239 Size: 268 1524 Fragments arranged by size: 1524 839 787 657 597 591 462 393 268 240 EcoO109I rG'GnC_Cy Cuts at: 3218 3233 3218 Size: 15 6343 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2418 2418 Size: 6358 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 3002 5505 1678 Size: 1324 2503 2531 Fragments arranged by size: 2531 2503 1324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 4999 5031 5073 Size: 32 135 32 608 32 2787 32 42 Cuts at: 5073 5105 1373 Size: 32 2626 Fragments arranged by size: 2787 2626 608 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2869 3289 3702 3948 669 Size: 988 281 931 420 413 246 3079 Fragments arranged by size: 3079 988 931 420 413 281 246 HindIII A'AGCT_T Cuts at: 748 2333 748 Size: 1585 4773 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3383 3683 3383 Size: 300 6058 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5472 5656 1305 Size: 4167 184 2007 Fragments arranged by size: 4167 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3501 10 Size: 54 1425 343 1669 2867 Fragments arranged by size: 2867 1669 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2087 2373 2515 4316 4675 4834 Size: 648 898 23 286 142 1801 359 159 Cuts at: 4834 518 Size: 2042 Fragments arranged by size: 2042 1801 898 648 359 286 159 142 23 MspA1I CmG'CkG Cuts at: 1606 3539 3774 4451 5599 5844 1606 Size: 1933 235 677 1148 245 2120 Fragments arranged by size: 2120 1933 1148 677 245 235 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5806 NdeI CA'TA_TG Cuts at: 387 2620 387 Size: 2233 4125 NheI G'CTAG_C Cuts at: 1052 2707 1052 Size: 1655 4703 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NsiI A_TGCA'T Cuts at: 2370 2370 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2092 3716 5261 1132 Size: 97 863 1624 1545 2229 Fragments arranged by size: 2229 1624 1545 863 97 PacI TTA_AT'TAA Cuts at: 2484 2484 Size: 6358 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6358 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PmlI CAC'GTG Cuts at: 3626 3626 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 2232 3928 2232 Size: 1696 4662 PspOMI G'GGCC_C Cuts at: 3217 3217 Size: 6358 PstI C_TGCA'G Cuts at: 830 3710 830 Size: 2880 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 SacI G_AGCT'C Cuts at: 2859 2859 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 3466 4606 6318 3466 Size: 1140 1712 3506 Fragments arranged by size: 3506 1712 1140 SexAI A'CCwGG_T Cuts at: 2446 2446 Size: 6358 SfcI C'TryA_G Cuts at: 826 1045 3706 4841 5522 5713 826 Size: 219 2661 1135 681 191 1471 Fragments arranged by size: 2661 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3216 3216 Size: 6358 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 3554 4462 5363 5625 820 Size: 197 926 1349 262 908 901 262 1553 Fragments arranged by size: 1553 1349 926 908 901 262 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 2863 152 Size: 2711 3647 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6358 StyI C'CwwG_G Cuts at: 513 1065 2454 3417 3583 3797 513 Size: 552 1389 963 166 214 3074 Fragments arranged by size: 3074 1389 963 552 214 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4385 4402 4555 4740 5185 325 Size: 1255 2805 17 153 185 445 1498 Fragments arranged by size: 2805 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3464 4604 6316 Size: 274 80 33 51 1443 1487 1140 1712 Cuts at: 6316 96 Size: 138 Fragments arranged by size: 1712 1487 1443 1140 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2434 2466 6318 6350 2434 Size: 32 3852 32 2442 Fragments arranged by size: 3852 2442 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 4487 4487 Size: 6358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4166 276 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfoI SrfI SwaI XcmI