Restriction map for
pFN21AA0761
(Linear) (Six-base) MAPSORT of: KIAA0761.seq Check: 7938 from: 1 to: 1653
KIAA0761.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1168 1653
Size: 1168 485
Acc65I G'GTAC_C
Cuts at: 0 1374 1653
Size: 1374 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 746 1337 1577 1653
Size: 89 657 591 240 76
Fragments arranged by size:
657 591 240 89 76
AfeI AGC'GCT
Cuts at: 0 995 1653
Size: 995 658
AflIII A'CryG_T
Cuts at: 0 642 1653
Size: 642 1011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 386 1653
Size: 386 1267
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1052 1084 1653
Size: 1052 32 569
Fragments arranged by size:
1052 569 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1646 1653
Size: 1646 7
ApaI G_GGCC'C
Cuts at: 0 1216 1653
Size: 1216 437
ApoI r'AATT_y
Cuts at: 0 961 986 1653
Size: 961 25 667
Fragments arranged by size:
961 667 25
AseI AT'TA_AT
Cuts at: 0 479 1653
Size: 479 1174
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1358 1391 1653
Size: 1358 33 262
Fragments arranged by size:
1358 262 33
BamHI G'GATC_C
Cuts at: 0 540 1629 1653
Size: 540 1089 24
Fragments arranged by size:
1089 540 24
BanI G'GyrC_C
Cuts at: 0 445 1374 1653
Size: 445 929 279
Fragments arranged by size:
929 445 279
BanII G_rGCy'C
Cuts at: 0 854 1216 1315 1653
Size: 854 362 99 338
Fragments arranged by size:
854 362 338 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 266 437 1143 1653
Size: 266 171 706 510
Fragments arranged by size:
706 510 266 171
BciVI GTATCCnnnnn_n'
Cuts at: 0 1395 1653
Size: 1395 258
BfrBI ATG'CAT
Cuts at: 0 363 1653
Size: 363 1290
BglI GCCn_nnn'nGGC
Cuts at: 0 1211 1649 1653
Size: 1211 438 4
Fragments arranged by size:
1211 438 4
Bme1580I G_kGCm'C
Cuts at: 0 1216 1653
Size: 1216 437
BmgBI CAC'GTC
Cuts at: 0 643 1653
Size: 643 1010
BmrI ACTGGGnnnn_n'
Cuts at: 0 965 1373 1653
Size: 965 408 280
Fragments arranged by size:
965 408 280
BmtI G_CTAG'C
Cuts at: 0 706 1653
Size: 706 947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 140 1124 1653
Size: 140 984 529
Fragments arranged by size:
984 529 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1308 1534 1653
Size: 1308 226 119
Fragments arranged by size:
1308 226 119
BsaAI yAC'GTr
Cuts at: 0 1621 1653
Size: 1621 32
BsaWI w'CCGG_w
Cuts at: 0 1632 1653
Size: 1632 21
BseYI C'CCAG_C
Cuts at: 0 1544 1653
Size: 1544 109
BsiHKAI G_wGCw'C
Cuts at: 0 854 1325 1653
Size: 854 471 328
Fragments arranged by size:
854 471 328
BsmI GAATG_Cn'
Cuts at: 0 31 1653
Size: 31 1622
BsmBI CGTCTCn'nnnn_
Cuts at: 0 633 1653
Size: 633 1020
Bsp1286I G_dGCh'C
Cuts at: 0 854 1216 1315 1325 1653
Size: 854 362 99 10 328
Fragments arranged by size:
854 362 328 99 10
BspHI T'CATG_A
Cuts at: 0 54 492 977 1653
Size: 54 438 485 676
Fragments arranged by size:
676 485 438 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1168 1653
Size: 1168 485
BssSI C'ACGA_G
Cuts at: 0 1355 1653
Size: 1355 298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1183 1653
Size: 1183 470
BstBI TT'CG_AA
Cuts at: 0 521 1653
Size: 521 1132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 771 1653
Size: 771 882
BstYI r'GATC_y
Cuts at: 0 540 1629 1653
Size: 540 1089 24
Fragments arranged by size:
1089 540 24
BtgI C'CryG_G
Cuts at: 0 1126 1367 1653
Size: 1126 241 286
Fragments arranged by size:
1126 286 241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1589 1624 1653
Size: 1589 35 29
Fragments arranged by size:
1589 35 29
DraIII CAC_nnn'GTG
Cuts at: 0 466 1653
Size: 466 1187
EaeI y'GGCC_r
Cuts at: 0 1123 1478 1494 1653
Size: 1123 355 16 159
Fragments arranged by size:
1123 355 159 16
EarI CTCTTCn'nnn_
Cuts at: 0 1251 1653
Size: 1251 402
EcoICRI GAG'CTC
Cuts at: 0 852 1653
Size: 852 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 746 1337 1577 1653
Size: 89 657 591 240 76
Fragments arranged by size:
657 591 240 89 76
EcoO109I rG'GnC_Cy
Cuts at: 0 1213 1228 1653
Size: 1213 15 425
Fragments arranged by size:
1213 425 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 413 1653
Size: 413 1240
HaeII r_GCGC'y
Cuts at: 0 997 1653
Size: 997 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1653
Size: 175 32 1446
Fragments arranged by size:
1446 175 32
HincII GTy'rAC
Cuts at: 0 864 1284 1653
Size: 864 420 369
Fragments arranged by size:
864 420 369
HindIII A'AGCT_T
Cuts at: 0 328 1653
Size: 328 1325
KpnI G_GTAC'C
Cuts at: 0 1378 1653
Size: 1378 275
MscI TGG'CCA
Cuts at: 0 1496 1653
Size: 1496 157
MslI CAynn'nnrTG
Cuts at: 0 59 82 368 510 1653
Size: 59 23 286 142 1143
Fragments arranged by size:
1143 286 142 59 23
MspA1I CmG'CkG
Cuts at: 0 1534 1653
Size: 1534 119
NdeI CA'TA_TG
Cuts at: 0 615 1653
Size: 615 1038
NheI G'CTAG_C
Cuts at: 0 702 1653
Size: 702 951
NsiI A_TGCA'T
Cuts at: 0 365 1653
Size: 365 1288
NspI r_CATG'y
Cuts at: 0 87 1653
Size: 87 1566
PacI TTA_AT'TAA
Cuts at: 0 479 1653
Size: 479 1174
PmlI CAC'GTG
Cuts at: 0 1621 1653
Size: 1621 32
PsiI TTA'TAA
Cuts at: 0 227 1653
Size: 227 1426
PspOMI G'GGCC_C
Cuts at: 0 1212 1653
Size: 1212 441
SacI G_AGCT'C
Cuts at: 0 854 1653
Size: 854 799
ScaI AGT'ACT
Cuts at: 0 1461 1653
Size: 1461 192
SexAI A'CCwGG_T
Cuts at: 0 441 1653
Size: 441 1212
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1211 1653
Size: 1211 442
SmlI C'TyrA_G
Cuts at: 0 1287 1549 1653
Size: 1287 262 104
Fragments arranged by size:
1287 262 104
SpeI A'CTAG_T
Cuts at: 0 858 1653
Size: 858 795
StyI C'CwwG_G
Cuts at: 0 449 1412 1578 1653
Size: 449 963 166 75
Fragments arranged by size:
963 449 166 75
TatI w'GTAC_w
Cuts at: 0 1459 1653
Size: 1459 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 429 461 1653
Size: 429 32 1192
Fragments arranged by size:
1192 429 32
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AloI AlwNI
ApaI ApoI AseI BaeI BamHI BanI BanII BbsI
BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaWI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BssSI BstAPI BstBI BstXI BstYI BtgI
CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII KpnI MscI MslI MspA1I
NdeI NheI NsiI NspI PacI PmlI PsiI PspOMI
SacI ScaI SexAI SfiI SmlI SpeI StyI TatI
TstI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI ApaLI
AscI AsiSI AvaI AvrII BbeI BbvCI BcgI BclI
BglII BlpI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BsgI BsiEI BsiWI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
DraI DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NgoMIV NotI NruI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SfcI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI