(Circular) (Six-base) MAPSORT of: pFN21AA0725.seq Check: 1089 from: 1 to: 6838 pFN21AA0725 6838 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3454 3454 Size: 6838 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 2572 4181 1937 Size: 370 265 1609 4594 Fragments arranged by size: 4594 1609 370 265 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2383 3179 3260 3363 3822 3864 3879 4086 Size: 751 796 81 103 459 42 15 207 Cuts at: 4086 4901 6285 6553 1632 Size: 815 1384 268 1917 Fragments arranged by size: 1917 1384 815 796 751 459 268 207 103 81 42 15 AflII C'TTAA_G Cuts at: 820 1017 3761 820 Size: 197 2744 3897 Fragments arranged by size: 3897 2744 197 AflIII A'CryG_T Cuts at: 1203 5737 6714 1203 Size: 4534 977 1327 Fragments arranged by size: 4534 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6838 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6838 AlwNI CAG_nnn'CTG Cuts at: 2203 2537 3869 4581 6153 2203 Size: 334 1332 712 1572 2888 Fragments arranged by size: 2888 1572 1332 712 334 ApaLI G'TGCA_C Cuts at: 4895 6051 4895 Size: 1156 5682 ApoI r'AATT_y Cuts at: 1493 2801 3206 3288 3671 4147 4373 4525 6634 Size: 1308 405 82 383 476 226 152 2109 Cuts at: 6634 1493 Size: 1697 Fragments arranged by size: 2109 1697 1308 476 405 383 226 152 82 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 4163 1870 Size: 73 2220 4545 Fragments arranged by size: 4545 2220 73 BamHI G'GATC_C Cuts at: 4168 4535 4168 Size: 367 6471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 5614 618 Size: 325 217 627 2372 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3817 4157 1391 Size: 2426 340 4072 Fragments arranged by size: 4072 2426 340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2917 928 Size: 971 1018 4849 Fragments arranged by size: 4849 1018 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5029 5063 1903 Size: 34 3092 34 3678 Fragments arranged by size: 3678 3092 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3049 4730 5940 3049 Size: 1681 1210 3947 Fragments arranged by size: 3947 1681 1210 BclI T'GATC_A Cuts at: 1548 4123 1548 Size: 2575 4263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5449 136 Size: 107 122 71 631 605 3777 1525 Fragments arranged by size: 3777 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2854 3271 4095 6833 2854 Size: 417 824 2738 2859 Fragments arranged by size: 2859 2738 824 417 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 4899 6055 1790 Size: 3109 1156 2573 Fragments arranged by size: 3109 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6838 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 2320 4066 Size: 416 53 83 399 24 618 285 1746 Cuts at: 4066 4569 5527 442 Size: 503 958 1753 Fragments arranged by size: 1753 1746 958 618 503 416 399 285 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5498 1239 Size: 4259 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4963 5828 6126 4963 Size: 865 298 5675 Fragments arranged by size: 5675 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3352 3905 5501 882 Size: 617 1853 553 1596 2219 Fragments arranged by size: 2219 1853 1596 617 553 BsaAI yAC'GTr Cuts at: 493 1204 2346 4629 493 Size: 711 1142 2283 2702 Fragments arranged by size: 2702 2283 1142 711 BsaBI GATnn'nnATC Cuts at: 2853 6832 2853 Size: 3979 2859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4645 5027 5662 275 Size: 53 83 186 936 3112 382 635 1451 Fragments arranged by size: 3112 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5271 5669 5943 6090 6501 5271 Size: 398 274 147 411 5608 Fragments arranged by size: 5608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 3799 3829 1178 Size: 30 165 30 618 30 1748 30 4187 Fragments arranged by size: 4187 1748 618 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2607 1186 Size: 1421 5417 BseYI C'CCAG_C Cuts at: 2876 6041 6537 2876 Size: 3165 496 3177 Fragments arranged by size: 3177 3165 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 1163 Size: 573 300 5965 Fragments arranged by size: 5965 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3817 4899 4984 6055 3817 Size: 1082 85 1071 4600 Fragments arranged by size: 4600 1082 1071 85 BsmI GAATG_Cn' Cuts at: 2833 4349 4442 2833 Size: 1516 93 5229 Fragments arranged by size: 5229 1516 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2329 2329 Size: 6838 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3817 4157 4899 4984 6055 1391 Size: 399 2027 340 742 85 1071 2174 Fragments arranged by size: 2174 2027 1071 742 399 340 85 BspEI T'CCGG_A Cuts at: 6501 6501 Size: 6838 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3454 4193 844 Size: 2610 739 3489 Fragments arranged by size: 3489 2610 739 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3042 5333 5507 65 Size: 1183 1794 2291 174 1396 Fragments arranged by size: 2291 1794 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5482 5669 1700 Size: 110 3672 187 2869 Fragments arranged by size: 3672 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2707 3260 96 Size: 2611 553 3674 Fragments arranged by size: 3674 2611 553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6838 BstBI TT'CG_AA Cuts at: 2698 5642 2698 Size: 2944 3894 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6838 BstXI CCAn_nnnn'nTGG Cuts at: 2672 2817 2672 Size: 145 6693 BstYI r'GATC_y Cuts at: 1972 2047 2854 3271 3521 4095 4168 4535 4920 Size: 75 807 417 250 574 73 367 385 Cuts at: 4920 4937 6389 6475 6487 6833 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 807 574 417 385 367 346 250 86 75 73 17 12 BstZ17I GTA'TAC Cuts at: 2308 2308 Size: 6838 Bsu36I CC'TnA_GG Cuts at: 2891 2891 Size: 6838 BtgI C'CryG_G Cuts at: 513 1065 2559 2665 513 Size: 552 1494 106 4686 Fragments arranged by size: 4686 1494 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 2737 4354 5148 5168 2737 Size: 1617 794 20 4407 Fragments arranged by size: 4407 1617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 4142 4142 Size: 6838 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5845 809 Size: 507 4529 1802 Fragments arranged by size: 4529 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4285 5174 6803 Size: 54 1425 50 275 18 2455 889 1629 Cuts at: 6803 8 Size: 43 Fragments arranged by size: 2455 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 2582 3903 4768 1353 Size: 849 380 1321 865 3423 Fragments arranged by size: 3423 1321 865 849 380 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3815 3815 Size: 6838 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2383 3179 3260 3363 3822 3864 3879 Size: 393 751 796 81 103 459 42 15 Cuts at: 3879 4086 4901 5498 6285 6553 1239 Size: 207 815 597 787 268 1524 Fragments arranged by size: 1524 815 796 787 751 597 459 393 268 207 103 81 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 3378 3378 Size: 6838 EcoRI G'AATT_C Cuts at: 3206 3288 4147 4525 3206 Size: 82 859 378 5519 Fragments arranged by size: 5519 859 378 82 EcoRV GAT'ATC Cuts at: 3082 3217 3082 Size: 135 6703 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3787 3787 Size: 6838 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 3679 3711 5479 Size: 32 135 32 449 32 1626 32 1768 Cuts at: 5479 5511 5553 5585 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1768 1626 449 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2573 2614 2815 3901 4182 4428 Size: 988 281 635 41 201 1086 281 246 Cuts at: 4428 669 Size: 3079 Fragments arranged by size: 3079 1086 988 635 281 281 246 201 41 HindIII A'AGCT_T Cuts at: 748 2504 3834 748 Size: 1756 1330 3752 Fragments arranged by size: 3752 1756 1330 HpaI GTT'AAC Cuts at: 3901 4428 3901 Size: 527 6311 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3611 5952 6136 1305 Size: 2306 2341 184 2007 Fragments arranged by size: 2341 2306 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5016 Fragments arranged by size: 5016 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3533 4796 5155 5314 518 Size: 648 2367 1263 359 159 2042 Fragments arranged by size: 2367 2042 1263 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2203 4254 4931 6079 6324 1606 Size: 597 2051 677 1148 245 2120 Fragments arranged by size: 2120 2051 1148 677 597 245 NcoI C'CATG_G Cuts at: 513 1065 2559 2665 513 Size: 552 1494 106 4686 Fragments arranged by size: 4686 1494 552 106 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 2428 4196 5741 1132 Size: 97 1199 1768 1545 2229 Fragments arranged by size: 2229 1768 1545 1199 97 PasI CC'CwG_GG Cuts at: 1348 4108 1348 Size: 2760 4078 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6838 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2978 3010 2978 Size: 32 6806 PstI C_TGCA'G Cuts at: 830 2862 3416 4190 830 Size: 2032 554 774 3478 Fragments arranged by size: 3478 2032 774 554 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 PvuII CAG'CTG Cuts at: 2203 2203 Size: 6838 SacI G_AGCT'C Cuts at: 3817 3817 Size: 6838 SalI G'TCGA_C Cuts at: 1936 2571 4180 1936 Size: 635 1609 4594 Fragments arranged by size: 4594 1609 635 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SfcI C'TryA_G Cuts at: 826 1045 2750 2858 3008 3412 3577 3637 3992 Size: 219 1705 108 150 404 165 60 355 Cuts at: 3992 4186 5321 6002 6193 826 Size: 194 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 681 404 355 219 194 191 165 150 108 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 4165 1872 Size: 2293 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3761 4942 5843 6105 820 Size: 197 926 1818 1181 901 262 1553 Fragments arranged by size: 1818 1553 1181 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 4196 1132 Size: 3064 3774 SspI AAT'ATT Cuts at: 5 52 3142 3610 4762 5 Size: 47 3090 468 1152 2081 Fragments arranged by size: 3090 2081 1152 468 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6838 StyI C'CwwG_G Cuts at: 513 1065 2559 2665 4277 513 Size: 552 1494 106 1612 3074 Fragments arranged by size: 3074 1612 1494 552 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 3282 4865 4882 5035 5220 5665 Size: 1255 992 710 1583 17 153 185 445 Cuts at: 5665 325 Size: 1498 Fragments arranged by size: 1583 1498 1255 992 710 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2707 3260 5084 Size: 274 80 33 51 1443 730 553 1824 Cuts at: 5084 6796 96 Size: 1712 138 Fragments arranged by size: 1824 1712 1443 730 553 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6798 6830 6798 Size: 32 6806 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3751 3751 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 4163 1870 Size: 2293 4545 XmnI GAAnn'nnTTC Cuts at: 2805 4967 2805 Size: 2162 4676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI