(Circular) (Six-base) MAPSORT of: pFN21AA0721.seq Check: 830 from: 1 to: 5947 pFN21AA0721 5947 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 3290 1937 Size: 268 1085 4594 Fragments arranged by size: 4594 1085 268 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2666 3214 4010 5394 5662 1632 Size: 1034 548 796 1384 268 1917 Fragments arranged by size: 1917 1384 1034 796 548 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 4846 5823 1203 Size: 3643 977 1327 Fragments arranged by size: 3643 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2295 1184 Size: 1111 4836 AlwNI CAG_nnn'CTG Cuts at: 2315 3690 5262 2315 Size: 1375 1572 3000 Fragments arranged by size: 3000 1572 1375 ApaI G_GGCC'C Cuts at: 2596 3177 2596 Size: 581 5366 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 2888 3098 3186 3256 3482 3634 5743 1493 Size: 1395 210 88 70 226 152 2109 1697 Fragments arranged by size: 2109 1697 1395 226 210 152 88 70 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 2197 3277 3644 2197 Size: 1080 367 4500 Fragments arranged by size: 4500 1080 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3000 3268 4723 618 Size: 325 217 627 1213 268 1455 1842 Fragments arranged by size: 1842 1455 1213 627 325 268 217 BanII G_rGCy'C Cuts at: 1391 2112 2370 2596 3177 3266 1391 Size: 721 258 226 581 89 4072 Fragments arranged by size: 4072 721 581 258 226 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 4046 Fragments arranged by size: 4046 971 930 BbvCI CC'TCA_GC Cuts at: 2831 2831 Size: 5947 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2082 2116 3092 3126 4138 4172 1903 Size: 34 145 34 976 34 1012 34 3678 Fragments arranged by size: 3678 1012 976 145 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 2805 2841 1548 Size: 1257 36 4654 Fragments arranged by size: 4654 1257 36 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 2483 4558 Size: 107 122 71 631 605 652 159 2075 Cuts at: 4558 136 Size: 1525 Fragments arranged by size: 2075 1525 652 631 605 159 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2596 3177 4008 5164 1790 Size: 806 581 831 1156 2573 Fragments arranged by size: 2573 1156 831 806 581 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2225 3678 4636 Size: 416 53 83 399 24 808 1453 958 Cuts at: 4636 442 Size: 1753 Fragments arranged by size: 1753 1453 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2645 4607 1239 Size: 947 459 1962 2579 Fragments arranged by size: 2579 1962 947 459 Bpu10I CC'TnA_GC Cuts at: 2831 2831 Size: 5947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 4072 4937 5235 2708 Size: 1364 865 298 3420 Fragments arranged by size: 3420 1364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2088 2162 2430 4610 882 Size: 617 589 74 268 2180 2219 Fragments arranged by size: 2219 2180 617 589 268 74 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 4380 4778 5052 5199 5610 2086 Size: 2294 398 274 147 411 2423 Fragments arranged by size: 2423 2294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 2873 1186 Size: 1405 282 4260 Fragments arranged by size: 4260 1405 282 BseYI C'CCAG_C Cuts at: 2381 2790 5150 5646 2381 Size: 409 2360 496 2682 Fragments arranged by size: 2682 2360 496 409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 2561 1163 Size: 573 538 287 4549 Fragments arranged by size: 4549 573 538 287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4008 4093 5164 4008 Size: 85 1071 4791 Fragments arranged by size: 4791 1071 85 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2370 2596 3177 3266 4008 4093 Size: 399 322 258 226 581 89 742 85 Cuts at: 4093 5164 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 581 399 322 258 226 89 85 BspEI T'CCGG_A Cuts at: 2086 5610 2086 Size: 3524 2423 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3834 1394 Size: 429 187 1824 3507 Fragments arranged by size: 3507 1824 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 4591 4778 1700 Size: 110 238 2543 187 2869 Fragments arranged by size: 2869 2543 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 2315 794 Size: 1521 4426 BstBI TT'CG_AA Cuts at: 2779 4751 2779 Size: 1972 3975 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2348 2763 2997 2348 Size: 415 234 5298 Fragments arranged by size: 5298 415 234 BstYI r'GATC_y Cuts at: 1972 2083 2197 2957 3224 3277 3644 4029 4046 Size: 111 114 760 267 53 367 385 17 Cuts at: 4046 5498 5584 5596 5942 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 267 114 111 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2077 2077 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 2371 513 Size: 552 1306 4089 Fragments arranged by size: 4089 1306 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2534 487 Size: 683 56 1308 3900 Fragments arranged by size: 3900 1308 683 56 BtsI GCAGTG_nn' Cuts at: 2496 3463 4257 4277 2496 Size: 967 794 20 4166 Fragments arranged by size: 4166 967 794 20 ClaI AT'CG_AT Cuts at: 1554 2634 1554 Size: 1080 4867 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2701 2812 2965 Size: 54 1425 50 275 18 871 111 153 Cuts at: 2965 3394 4283 5912 8 Size: 429 889 1629 43 Fragments arranged by size: 1629 1425 889 871 429 275 153 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3877 1353 Size: 2524 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2645 2666 3214 4010 4607 5394 Size: 393 554 459 21 548 796 597 787 Cuts at: 5394 5662 1239 Size: 268 1524 Fragments arranged by size: 1524 796 787 597 554 548 459 393 268 21 EcoO109I rG'GnC_Cy Cuts at: 2592 2593 2673 3173 3174 2592 Size: 1 80 500 1 5365 Fragments arranged by size: 5365 500 80 1 1 EcoRI G'AATT_C Cuts at: 3098 3186 3256 3634 3098 Size: 88 70 378 5411 Fragments arranged by size: 5411 378 88 70 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 3222 5094 1678 Size: 1544 1872 2531 Fragments arranged by size: 2531 1872 1544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 4588 4620 4662 Size: 32 135 32 488 32 2496 32 42 Cuts at: 4662 4694 1373 Size: 32 2626 Fragments arranged by size: 2626 2496 488 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3291 3537 669 Size: 988 281 268 1085 246 3079 Fragments arranged by size: 3079 1085 988 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5947 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2814 10 Size: 54 1425 343 982 3143 Fragments arranged by size: 3143 1425 982 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3905 4264 4423 518 Size: 648 2739 359 159 2042 Fragments arranged by size: 2739 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2794 2997 3363 4040 5188 5433 1606 Size: 1188 203 366 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 677 366 245 203 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2710 2725 2830 3305 4850 1132 Size: 97 1481 15 105 475 1545 2229 Fragments arranged by size: 2229 1545 1481 475 105 97 15 PasI CC'CwG_GG Cuts at: 1348 2589 1348 Size: 1241 4706 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PspOMI G'GGCC_C Cuts at: 2592 3173 2592 Size: 581 5366 PstI C_TGCA'G Cuts at: 830 3299 830 Size: 2469 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 PvuII CAG'CTG Cuts at: 2794 2794 Size: 5947 RsrII CG'GwC_CG Cuts at: 2089 2089 Size: 5947 SalI G'TCGA_C Cuts at: 1936 2204 3289 1936 Size: 268 1085 4594 Fragments arranged by size: 4594 1085 268 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 3295 4430 5111 5302 826 Size: 219 2250 1135 681 191 1471 Fragments arranged by size: 2250 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 4051 4952 5214 820 Size: 197 926 744 1364 901 262 1553 Fragments arranged by size: 1553 1364 926 901 744 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 2710 3305 1132 Size: 1578 595 3774 Fragments arranged by size: 3774 1578 595 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 2104 2451 3200 3386 513 Size: 552 1039 347 749 186 3074 Fragments arranged by size: 3074 1039 749 552 347 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 3155 3974 3991 4144 4329 4774 Size: 1255 526 1049 819 17 153 185 445 Cuts at: 4774 325 Size: 1498 Fragments arranged by size: 1498 1255 1049 819 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4193 5905 96 Size: 274 80 33 51 1443 2216 1712 138 Fragments arranged by size: 2216 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 2395 3094 3283 2395 Size: 699 189 5059 Fragments arranged by size: 5059 699 189 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI