(Circular) (Six-base) MAPSORT of: pFN21AA0713.seq Check: 9909 from: 1 to: 7576 pFN21AA0713 7576 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5386 278 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 4919 1937 Size: 1204 1778 4594 Fragments arranged by size: 4594 1778 1204 Acc65I G'GTAC_C Cuts at: 4897 4897 Size: 7576 AclI AA'CG_TT Cuts at: 1563 4510 5703 6076 1563 Size: 2947 1193 373 3063 Fragments arranged by size: 3063 2947 1193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3496 4658 5639 7023 7291 1632 Size: 1864 1162 981 1384 268 1917 Fragments arranged by size: 1917 1864 1384 1162 981 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7379 AflIII A'CryG_T Cuts at: 1203 3555 6475 7452 1203 Size: 2352 2920 977 1327 Fragments arranged by size: 2920 2352 1327 977 AgeI A'CCGG_T Cuts at: 6407 6407 Size: 7576 AhdI GACnn_n'nnGTC Cuts at: 6305 6305 Size: 7576 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7576 AlwNI CAG_nnn'CTG Cuts at: 3329 3961 4265 4553 5319 6891 3329 Size: 632 304 288 766 1572 4014 Fragments arranged by size: 4014 1572 766 632 304 288 ApaLI G'TGCA_C Cuts at: 2644 5633 6789 2644 Size: 2989 1156 3431 Fragments arranged by size: 3431 2989 1156 ApoI r'AATT_y Cuts at: 1493 2775 3336 3634 4775 4885 5111 5263 7372 Size: 1282 561 298 1141 110 226 152 2109 Cuts at: 7372 1493 Size: 1697 Fragments arranged by size: 2109 1697 1282 1141 561 298 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 2789 2820 4443 6130 160 Size: 872 1757 31 1623 1687 1606 Fragments arranged by size: 1757 1687 1623 1606 872 31 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 AvaI C'yCGr_G Cuts at: 1870 1943 4901 1870 Size: 73 2958 4545 Fragments arranged by size: 4545 2958 73 BamHI G'GATC_C Cuts at: 3581 4906 5273 3581 Size: 1325 367 5884 Fragments arranged by size: 5884 1325 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 4231 4897 6352 618 Size: 325 217 627 504 1940 666 1455 1842 Fragments arranged by size: 1940 1842 1455 666 627 504 325 217 BanII G_rGCy'C Cuts at: 1391 4895 1391 Size: 3504 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4726 928 Size: 971 2827 3778 Fragments arranged by size: 3778 2827 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5767 5801 1903 Size: 34 3830 34 3678 Fragments arranged by size: 3830 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3221 5468 6678 3221 Size: 2247 1210 4119 Fragments arranged by size: 4119 2247 1210 BclI T'GATC_A Cuts at: 1548 2554 3721 1548 Size: 1006 1167 5403 Fragments arranged by size: 5403 1167 1006 BfrBI ATG'CAT Cuts at: 3740 3947 4523 3740 Size: 207 576 6793 Fragments arranged by size: 6793 576 207 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6187 136 Size: 107 122 71 631 605 4515 1525 Fragments arranged by size: 4515 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2578 2957 7571 2578 Size: 379 4614 2583 Fragments arranged by size: 4614 2583 379 BlpI GC'TnA_GC Cuts at: 3546 4993 3546 Size: 1447 6129 Bme1580I G_kGCm'C Cuts at: 1790 2648 5637 6793 1790 Size: 858 2989 1156 2573 Fragments arranged by size: 2989 2573 1156 858 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5307 6265 442 Size: 416 53 83 399 24 3890 958 1753 Fragments arranged by size: 3890 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7576 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2739 3207 6236 1239 Size: 1061 439 468 3029 2579 Fragments arranged by size: 3029 2579 1061 468 439 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2359 3025 3847 4043 4836 5701 6566 6864 2359 Size: 666 822 196 793 865 865 298 3071 Fragments arranged by size: 3071 865 865 822 793 666 298 196 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3621 6239 882 Size: 617 2122 2618 2219 Fragments arranged by size: 2618 2219 2122 617 BsaAI yAC'GTr Cuts at: 493 1204 5367 493 Size: 711 4163 2702 Fragments arranged by size: 4163 2702 711 BsaBI GATnn'nnATC Cuts at: 7570 7570 Size: 7576 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5383 5765 6400 275 Size: 53 83 186 936 3850 382 635 1451 Fragments arranged by size: 3850 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6009 6407 6681 6828 7239 6009 Size: 398 274 147 411 6346 Fragments arranged by size: 6346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7351 Fragments arranged by size: 7351 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3797 1186 Size: 2611 4965 BseYI C'CCAG_C Cuts at: 3991 6779 7275 3991 Size: 2788 496 4292 Fragments arranged by size: 4292 2788 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7003 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5026 5787 6815 1540 Size: 275 186 3025 761 1028 2301 Fragments arranged by size: 3025 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2648 5637 5722 6793 2648 Size: 2989 85 1071 3431 Fragments arranged by size: 3431 2989 1071 85 BsmI GAATG_Cn' Cuts at: 3132 3526 4801 5087 5180 3132 Size: 394 1275 286 93 5528 Fragments arranged by size: 5528 1275 394 286 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2648 4895 5637 5722 6793 1391 Size: 399 858 2247 742 85 1071 2174 Fragments arranged by size: 2247 2174 1071 858 742 399 85 BspEI T'CCGG_A Cuts at: 7239 7239 Size: 7576 BspHI T'CATG_A Cuts at: 3424 5465 7195 3424 Size: 2041 1730 3805 Fragments arranged by size: 3805 2041 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4931 844 Size: 4087 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5463 1394 Size: 429 3640 3507 Fragments arranged by size: 3640 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6071 6245 65 Size: 1183 4823 174 1396 Fragments arranged by size: 4823 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3762 6220 6407 1700 Size: 110 1952 2458 187 2869 Fragments arranged by size: 2869 2458 1952 187 110 BsrGI T'GTAC_A Cuts at: 96 3757 96 Size: 3661 3915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7576 BssSI C'ACGA_G Cuts at: 2769 5636 6648 2769 Size: 2867 1012 3697 Fragments arranged by size: 3697 2867 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3746 794 Size: 2952 4624 BstBI TT'CG_AA Cuts at: 4579 6380 4579 Size: 1801 5775 BstEII G'GTnAC_C Cuts at: 1184 3235 1184 Size: 2051 5525 BstYI r'GATC_y Cuts at: 1972 2578 2957 3430 3565 3581 3937 4906 5273 Size: 606 379 473 135 16 356 969 367 Cuts at: 5273 5658 5675 7127 7213 7225 7571 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 969 606 473 385 379 367 356 346 135 86 17 16 12 Bsu36I CC'TnA_GG Cuts at: 2675 2675 Size: 7576 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7024 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2391 4256 487 Size: 683 56 1165 1865 3807 Fragments arranged by size: 3807 1865 1165 683 56 BtsI GCAGTG_nn' Cuts at: 5092 5886 5906 5092 Size: 794 20 6762 Fragments arranged by size: 6762 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7576 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7541 DraI TTT'AAA Cuts at: 4880 4880 Size: 7576 DraIII CAC_nnn'GTG Cuts at: 4009 4009 Size: 7576 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4165 6583 809 Size: 507 2849 2418 1802 Fragments arranged by size: 2849 2418 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4529 5023 5912 Size: 54 1425 50 275 18 2699 494 889 Cuts at: 5912 7541 8 Size: 1629 43 Fragments arranged by size: 2699 1629 1425 889 494 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5023 1537 Size: 275 3211 4090 Fragments arranged by size: 4090 3211 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2418 5506 1353 Size: 1065 3088 3423 Fragments arranged by size: 3423 3088 1065 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6163 6537 6683 387 Size: 1064 138 4574 374 146 1280 Fragments arranged by size: 4574 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2739 3207 3496 4658 5639 6236 Size: 393 668 439 468 289 1162 981 597 Cuts at: 6236 7023 7291 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1162 981 787 668 597 468 439 393 289 268 EcoRI G'AATT_C Cuts at: 2775 4885 5263 2775 Size: 2110 378 5088 Fragments arranged by size: 5088 2110 378 EcoRV GAT'ATC Cuts at: 2983 2983 Size: 7576 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4694 4694 Size: 7576 FspI TGC'GCA Cuts at: 3536 6082 3536 Size: 2546 5030 FspAI rTGC'GCAy Cuts at: 3536 3536 Size: 7576 HaeII r_GCGC'y Cuts at: 1678 4859 6723 1678 Size: 3181 1864 2531 Fragments arranged by size: 3181 2531 1864 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2736 2768 3587 Size: 32 135 32 797 32 335 32 819 Cuts at: 3587 3619 6217 6249 6291 6323 1373 Size: 32 2598 32 42 32 2626 Fragments arranged by size: 2626 2598 819 797 335 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 4459 4468 4920 5166 669 Size: 988 281 72 2449 9 452 246 3079 Fragments arranged by size: 3079 2449 988 452 281 246 72 9 HindIII A'AGCT_T Cuts at: 748 748 Size: 7576 HpaI GTT'AAC Cuts at: 2010 5166 2010 Size: 3156 4420 KpnI G_GTAC'C Cuts at: 4901 4901 Size: 7576 MfeI C'AATT_G Cuts at: 5175 5175 Size: 7576 MluI A'CGCG_T Cuts at: 7452 7452 Size: 7576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2234 3313 3458 6690 6874 1305 Size: 929 1079 145 3232 184 2007 Fragments arranged by size: 3232 2007 1079 929 184 145 MscI TGG'CCA Cuts at: 10 64 1489 1832 4531 10 Size: 54 1425 343 2699 3055 Fragments arranged by size: 3055 2699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 5534 5893 6052 518 Size: 648 1671 2697 359 159 2042 Fragments arranged by size: 2697 2042 1671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2972 3326 4992 5669 6817 7062 1606 Size: 1366 354 1666 677 1148 245 2120 Fragments arranged by size: 2120 1666 1366 1148 677 354 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7024 NdeI CA'TA_TG Cuts at: 387 2030 2834 3736 4429 387 Size: 1643 804 902 693 3534 Fragments arranged by size: 3534 1643 902 804 693 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7576 NotI GC'GGCC_GC Cuts at: 5023 5023 Size: 7576 NsiI A_TGCA'T Cuts at: 3742 3949 4525 3742 Size: 207 576 6793 Fragments arranged by size: 6793 576 207 NspI r_CATG'y Cuts at: 1132 1229 3178 3541 3559 4934 6479 1132 Size: 97 1949 363 18 1375 1545 2229 Fragments arranged by size: 2229 1949 1545 1375 363 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7576 PciI A'CATG_T Cuts at: 3555 6475 3555 Size: 2920 4656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7576 PfoI T'CCnGG_A Cuts at: 2716 3880 2716 Size: 1164 6412 PmeI GTTT'AAAC Cuts at: 4880 4880 Size: 7576 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5645 5677 7158 7190 5645 Size: 32 1481 32 6031 Fragments arranged by size: 6031 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7576 PsiI TTA'TAA Cuts at: 5146 5146 Size: 7576 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4019 4051 4019 Size: 32 7544 PstI C_TGCA'G Cuts at: 830 4928 830 Size: 4098 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7576 PvuII CAG'CTG Cuts at: 2972 3326 2972 Size: 354 7222 SalI G'TCGA_C Cuts at: 1936 4918 1936 Size: 2982 4594 SbfI CC_TGCA'GG Cuts at: 4928 4928 Size: 7576 ScaI AGT'ACT Cuts at: 5824 7536 5824 Size: 1712 5864 SfcI C'TryA_G Cuts at: 826 1045 3621 4036 4924 6059 6740 6931 826 Size: 219 2576 415 888 1135 681 191 1471 Fragments arranged by size: 2576 1471 1135 888 681 415 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7576 SgrAI Cr'CCGG_yG Cuts at: 1700 3762 1700 Size: 2062 5514 SmaI CCC'GGG Cuts at: 1872 4903 1872 Size: 3031 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 3040 3862 4022 4851 5680 Size: 197 926 395 702 822 160 829 829 Cuts at: 5680 6581 6843 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 829 829 822 702 395 262 197 160 SnaBI TAC'GTA Cuts at: 493 5367 493 Size: 4874 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7576 SphI G_CATG'C Cuts at: 1132 4934 1132 Size: 3802 3774 SspI AAT'ATT Cuts at: 5 52 2091 2902 4315 5500 5 Size: 47 2039 811 1413 1185 2081 Fragments arranged by size: 2081 2039 1413 1185 811 47 StuI AGG'CCT Cuts at: 1517 2870 1517 Size: 1353 6223 StyI C'CwwG_G Cuts at: 513 1065 2047 5015 513 Size: 552 982 2968 3074 Fragments arranged by size: 3074 2968 982 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 5603 5620 5773 5958 6403 325 Size: 1255 534 3489 17 153 185 445 1498 Fragments arranged by size: 3489 1498 1255 534 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3679 3757 5822 Size: 274 80 33 51 1443 1702 78 2065 Cuts at: 5822 7534 96 Size: 1712 138 Fragments arranged by size: 2065 1712 1702 1443 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2830 2862 7536 7568 2830 Size: 32 4674 32 2838 Fragments arranged by size: 4674 2838 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7576 XbaI T'CTAG_A Cuts at: 2962 4912 2962 Size: 1950 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7576 XmaI C'CCGG_G Cuts at: 1870 4901 1870 Size: 3031 4545 XmnI GAAnn'nnTTC Cuts at: 2388 4511 4695 5705 2388 Size: 2123 184 1010 4259 Fragments arranged by size: 4259 2123 1010 184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5384 276 Size: 53 83 186 936 3850 2468 Fragments arranged by size: 3850 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI