Restriction map for
pFN21AA0660
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 78 TCCGGA T^CCGGA
AclI 1066 AACGTT AA^CGTT
AlwI 352 GGATC GGATC(4/5)
Asp700I 1147 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1436 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 168 TGGCCA TGG^CCA
BbrPI 1310 CACGTG CAC^GTG
BsaAI 1310 CACGTG [CT]AC^GT[GA]
BsaBI 985 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 78 TCCGGA [AT]^CCGG[AT]
BseAI 78 TCCGGA T^CCGGA
BsmAI 817 GTCTC GTCTC(1/5)
BsmFI 1298 GTCCC GGGAC(10/14)
BspEI 78 TCCGGA T^CCGGA
BspMI 758 ACCTGC ACCTGC(4/8)
BsrDI 1286 GCAATG GCAATG(2/0)
BssSI 866 CTCGTG CACGAG(-5/-1)
BstF5I 240 GGATG GGATG(2/0)
BstYI 352 GGATCT [GA]^GATC[CT]
CfoI 1284 GCGC GCG^C
DraI 1203 TTTAAA TTT^AAA
EaeI 168 TGGCCA [CT]^GGCC[GA]
EcoNI 907 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 36 GCGGG CCCGC(4/6)
FokI 240 GGATG GGATG(9/13)
HgaI 1440 GCGTC GACGC(5/10)
HhaI 1284 GCGC GCG^C
HinP1I 1284 GCGC G^CGC
HindIII 1108 AAGCTT A^AGCTT
MamI 985 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 352 GGATCT [GA]^GATC[CT]
MluNI 168 TGGCCA TGG^CCA
MroI 78 TCCGGA T^CCGGA
MscI 168 TGGCCA TGG^CCA
MslI 235 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 22 CCGCTG C[AC]G^C[GT]G
PflFI 1436 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1310 CACGTG CAC^GTG
PmlI 1310 CACGTG CAC^GTG
Psp1406I 1066 AACGTT AA^CGTT
SapI 619 GAAGAGC GCTCTTC(1/4)
SexAI 897 ACCTGGT A^CC[AT]GGT
SmlI 255 CTTGAG C^T[CT][GA]AG
Tth111I 1436 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 352 GGATCT [GA]^GATC[CT]
XmnI 1147 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 773 GTAC GT^AC
1198 GTAC
AlwNI 599 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
692 CAGAACCTG
BbvI 24 GCTGC GCAGC(8/12)
1231 GCTGC
BcnI 1300 CCCGG CC^[GC]GG
1409 CCGGG
BspHI 579 TCATGA T^CATGA
1052 TCATGA
DraII 1296 AGGTCCC [GA]G^G[ACGT]CC[CT]
1356 AGGACCT
DraIII 535 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
863 CACCTCGTG
EcoO109I 1296 AGGTCCC [GA]G^G[ACGT]CC[CT]
1356 AGGACCT
EcoT14I 1091 CCAAGG C^C[AT][AT]GG
1366 CCAAGG
HaeIII 169 GGCC GG^CC
1423 GGCC
MwoI 15 GCCCAGTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
27 GCTTGTAGGGC
NciI 1300 CCCGG CC^[GC]GG
1409 CCGGG
PpuMI 1296 AGGTCCC [GA]G^G[AT]CC[CT]
1356 AGGACCT
RcaI 579 TCATGA T^CATGA
1052 TCATGA
RsaI 773 GTAC GT^AC
1198 GTAC
SspI 53 AATATT AAT^ATT
83 AATATT
StyI 1091 CCAAGG C^C[AT][AT]GG
1366 CCAAGG
TseI 24 GCTGC G^C[AT]GC
1231 GCTGC
Tsp45I 541 GTGAC ^GT[GC]AC
745 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BfaI BsaJI BseRI BsiYI BslI
BsrI BssKI BstNI Cfr13I DdeI
DpnII EarI EcoRI EcoRII Fnu4HI
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
NdeII NlaIII NlaIV PflMI PleI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I Tsp509I TspRI TthHB8I
Van91I