(Circular) (Six-base) MAPSORT of: pFN21AA0660.seq Check: 8444 from: 1 to: 6151 pFN21AA0660 6151 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 3072 4278 4651 1563 Size: 1509 1206 373 3063 Fragments arranged by size: 3063 1509 1206 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 2373 2643 2730 4214 5598 5866 1632 Size: 565 176 270 87 1484 1384 268 1917 Fragments arranged by size: 1917 1484 1384 565 270 268 176 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2529 1184 Size: 1345 4806 AlwNI CAG_nnn'CTG Cuts at: 2609 2702 3894 5466 2609 Size: 93 1192 1572 3294 Fragments arranged by size: 3294 1572 1192 93 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2113 2232 2372 2796 3050 3121 3460 3686 Size: 620 119 140 424 254 71 339 226 Cuts at: 3686 3838 5947 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 620 424 339 254 226 152 140 119 71 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3470 1391 Size: 2079 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4043 5253 4043 Size: 1210 4941 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 4212 5368 1790 Size: 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2016 3882 4840 Size: 416 53 83 399 24 599 1866 958 Cuts at: 4840 442 Size: 1753 Fragments arranged by size: 1866 1753 958 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4811 1239 Size: 3572 2579 Bpu10I CC'TnA_GC Cuts at: 2598 2598 Size: 6151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2281 4276 5141 5439 2281 Size: 1995 865 298 2993 Fragments arranged by size: 2993 1995 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3317 3942 493 Size: 711 2113 625 2702 Fragments arranged by size: 2702 2113 711 625 BsaBI GATnn'nnATC Cuts at: 2994 6145 2994 Size: 3151 3000 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2083 4584 4982 5256 5403 5814 2083 Size: 2501 398 274 147 411 2420 Fragments arranged by size: 2501 2420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2262 2292 2671 2674 2701 Size: 30 165 30 859 30 379 3 27 Cuts at: 2701 2704 1178 Size: 3 4625 Fragments arranged by size: 4625 859 379 165 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2576 2660 2676 2679 2684 2952 3357 1186 Size: 1390 84 16 3 5 268 405 3980 Fragments arranged by size: 3980 1390 405 268 84 16 5 3 BseYI C'CCAG_C Cuts at: 5354 5850 5354 Size: 496 5655 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5578 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4212 4297 5368 4212 Size: 85 1071 4995 Fragments arranged by size: 4995 1071 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3470 4212 4297 5368 1391 Size: 399 1680 742 85 1071 2174 Fragments arranged by size: 2174 1680 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2083 5814 2083 Size: 3731 2420 BspHI T'CATG_A Cuts at: 2584 3057 4040 5770 2584 Size: 473 983 1730 2965 Fragments arranged by size: 2965 1730 983 473 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2772 3506 844 Size: 1928 734 3489 Fragments arranged by size: 3489 1928 734 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3298 4646 4820 65 Size: 1183 2050 1348 174 1396 Fragments arranged by size: 2050 1396 1348 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 2871 4211 5223 2871 Size: 1340 1012 3799 Fragments arranged by size: 3799 1340 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6151 BstYI r'GATC_y Cuts at: 1972 2357 3481 3848 4233 4250 5702 5788 5800 Size: 385 1124 367 385 17 1452 86 12 Cuts at: 5800 6146 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1124 385 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3210 3455 3210 Size: 245 5906 DraIII CAC_nnn'GTG Cuts at: 2545 2873 2545 Size: 328 5823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 3598 4487 Size: 54 1425 50 275 18 343 1425 889 Cuts at: 4487 6116 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 2619 3213 3352 3400 4081 1353 Size: 1107 159 594 139 48 681 3423 Fragments arranged by size: 3423 1107 681 594 159 139 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4738 5112 5258 387 Size: 1064 138 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2373 2643 2730 4214 4811 5598 Size: 393 565 176 270 87 1484 597 787 Cuts at: 5598 5866 1239 Size: 268 1524 Fragments arranged by size: 1524 1484 787 597 565 393 270 268 176 87 EcoNI CCTnn'n_nnAGG Cuts at: 2916 2916 Size: 6151 EcoO109I rG'GnC_Cy Cuts at: 3302 3362 3302 Size: 60 6091 EcoRI G'AATT_C Cuts at: 2113 2796 3050 3460 3838 2113 Size: 683 254 410 378 4426 Fragments arranged by size: 4426 683 410 378 254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3070 3070 Size: 6151 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 5298 1678 Size: 3620 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2262 2294 2432 2464 2591 Size: 32 135 32 690 32 138 32 127 Cuts at: 2591 2623 2660 2692 3331 3363 4792 4824 4866 Size: 32 37 32 639 32 1429 32 42 Cuts at: 4866 4898 1373 Size: 32 2626 Fragments arranged by size: 2626 1429 690 639 138 135 127 42 37 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 3113 748 Size: 2365 3786 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5265 5449 1305 Size: 3960 184 2007 Fragments arranged by size: 3960 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2175 10 Size: 54 1425 343 343 3986 Fragments arranged by size: 3986 1425 343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2244 4109 4468 4627 518 Size: 648 843 235 1865 359 159 2042 Fragments arranged by size: 2042 1865 843 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 2029 3567 4244 5392 5637 1606 Size: 423 1538 677 1148 245 2120 Fragments arranged by size: 2120 1538 1148 677 423 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 3509 5054 1132 Size: 97 2280 1545 2229 Fragments arranged by size: 2280 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2287 3125 3377 1108 Size: 1179 838 252 3882 Fragments arranged by size: 3882 1179 838 252 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PmlI CAC'GTG Cuts at: 3317 3317 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3302 3362 3302 Size: 60 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3721 3721 Size: 6151 PstI C_TGCA'G Cuts at: 830 3503 830 Size: 2673 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SapI GCTCTTCn'nnn_ Cuts at: 2619 2619 Size: 6151 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SexAI A'CCwGG_T Cuts at: 2902 2902 Size: 6151 SfcI C'TryA_G Cuts at: 826 1045 3499 4634 5315 5506 826 Size: 219 2454 1135 681 191 1471 Fragments arranged by size: 2454 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2260 4255 5156 5418 820 Size: 197 926 317 1995 901 262 1553 Fragments arranged by size: 1995 1553 926 901 317 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 2060 2090 4075 5 Size: 47 2008 30 1985 2081 Fragments arranged by size: 2081 2008 1985 47 30 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 3096 3371 3590 513 Size: 552 939 1092 275 219 3074 Fragments arranged by size: 3074 1092 939 552 275 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 4178 4195 4348 4533 4978 325 Size: 1255 1737 861 17 153 185 445 1498 Fragments arranged by size: 1737 1498 1255 861 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4397 6109 96 Size: 274 80 33 51 1443 2420 1712 138 Fragments arranged by size: 2420 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3060 3092 6111 6143 3060 Size: 32 3019 32 3068 Fragments arranged by size: 3068 3019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3444 1316 Size: 2128 4023 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 3156 4280 3156 Size: 1124 5027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI