Restriction map for
pFN21AA0660
(Linear) (Six-base) MAPSORT of: KIAA0660.seq Check: 4683 from: 1 to: 1446
KIAA0660.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AclI AA'CG_TT
Cuts at: 0 1067 1446
Size: 1067 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 368 638 725 1446
Size: 192 176 270 87 721
Fragments arranged by size:
721 270 192 176 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 1446
Size: 524 922
AlwNI CAG_nnn'CTG
Cuts at: 0 604 697 1446
Size: 604 93 749
Fragments arranged by size:
749 604 93
ApoI r'AATT_y
Cuts at: 0 108 227 367 791 1045 1116 1446
Size: 108 119 140 424 254 71 330
Fragments arranged by size:
424 330 254 140 119 108 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 11 1446
Size: 11 1435
Bpu10I CC'TnA_GC
Cuts at: 0 593 1446
Size: 593 853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 1446
Size: 276 1170
BsaAI yAC'GTr
Cuts at: 0 1312 1446
Size: 1312 134
BsaBI GATnn'nnATC
Cuts at: 0 989 1446
Size: 989 457
BsaWI w'CCGG_w
Cuts at: 0 78 1446
Size: 78 1368
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 666 669 696 699 1446
Size: 257 30 379 3 27 3 747
Fragments arranged by size:
747 379 257 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 655 671 674 679 947 1352 1446
Size: 571 84 16 3 5 268 405 94
Fragments arranged by size:
571 405 268 94 84 16 5 3
BspEI T'CCGG_A
Cuts at: 0 78 1446
Size: 78 1368
BspHI T'CATG_A
Cuts at: 0 579 1052 1446
Size: 579 473 394
Fragments arranged by size:
579 473 394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 1446
Size: 767 679
BsrDI GCAATG_nn'
Cuts at: 0 1293 1446
Size: 1293 153
BssSI C'ACGA_G
Cuts at: 0 866 1446
Size: 866 580
BstYI r'GATC_y
Cuts at: 0 352 1446
Size: 352 1094
DraI TTT'AAA
Cuts at: 0 1205 1446
Size: 1205 241
DraIII CAC_nnn'GTG
Cuts at: 0 540 868 1446
Size: 540 328 578
Fragments arranged by size:
578 540 328
EaeI y'GGCC_r
Cuts at: 0 168 1446
Size: 168 1278
EarI CTCTTCn'nnn_
Cuts at: 0 455 614 1208 1347 1395 1446
Size: 455 159 594 139 48 51
Fragments arranged by size:
594 455 159 139 51 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 368 638 725 1446
Size: 192 176 270 87 721
Fragments arranged by size:
721 270 192 176 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 911 1446
Size: 911 535
EcoO109I rG'GnC_Cy
Cuts at: 0 1297 1357 1446
Size: 1297 60 89
Fragments arranged by size:
1297 89 60
EcoRI G'AATT_C
Cuts at: 0 108 791 1045 1446
Size: 108 683 254 401
Fragments arranged by size:
683 401 254 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1065 1446
Size: 1065 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 427 459 586 618 655 687
Size: 257 32 138 32 127 32 37 32
Cuts at: 687 1326 1358 1446
Size: 639 32 88
Fragments arranged by size:
639 257 138 127 88 37 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1108 1446
Size: 1108 338
MscI TGG'CCA
Cuts at: 0 170 1446
Size: 170 1276
MslI CAynn'nnrTG
Cuts at: 0 239 1446
Size: 239 1207
MspA1I CmG'CkG
Cuts at: 0 24 1446
Size: 24 1422
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 1120 1372 1446
Size: 282 838 252 74
Fragments arranged by size:
838 282 252 74
PmlI CAC'GTG
Cuts at: 0 1312 1446
Size: 1312 134
PpuMI rG'GwC_Cy
Cuts at: 0 1297 1357 1446
Size: 1297 60 89
Fragments arranged by size:
1297 89 60
SapI GCTCTTCn'nnn_
Cuts at: 0 614 1446
Size: 614 832
SexAI A'CCwGG_T
Cuts at: 0 897 1446
Size: 897 549
SmlI C'TyrA_G
Cuts at: 0 255 1446
Size: 255 1191
SspI AAT'ATT
Cuts at: 0 55 85 1446
Size: 55 30 1361
Fragments arranged by size:
1361 55 30
StyI C'CwwG_G
Cuts at: 0 1091 1366 1446
Size: 1091 275 80
Fragments arranged by size:
1091 275 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1312 1446
Size: 1312 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 1446
Size: 1055 32 359
Fragments arranged by size:
1055 359 32
Tth111I GACn'n_nGTC
Cuts at: 0 1439 1446
Size: 1439 7
XmnI GAAnn'nnTTC
Cuts at: 0 1151 1446
Size: 1151 295
Enzymes that do cut:
AclI AcuI AlfI AlwNI ApoI BmrI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BspEI BspHI BspMI
BsrDI BssSI BstYI DraI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HindIII MscI MslI
MspA1I PflMI PmlI PpuMI SapI SexAI SmlI SspI
StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI BsaI BsaHI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI XbaI XcmI XhoI XmaI ZraI