Restriction map for
pFN21AA0622
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp718I AviII BbeI BbrPI BcgI
BciVI BglI BlpI Bpu1102I BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI Bst1107I BstZ17I CelII
ClaI CpoI DraIII DrdI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacI SbfI ScaI SfiI SfoI
SgrAI SpeI Sse8387I SspBI StuI
SwaI XcmI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 4162 GTCGAC GT^[AC][GT]AC
AclI 805 AACGTT AA^CGTT
AflII 2722 CTTAAG C^TTAAG
AocI 1907 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2402 AGCGCT AGC^GCT
ApaI 4334 GGGCCC GGGCC^C
Asp700I 2512 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1327 CCTAGG C^CTAGG
BbvCI 3994 GCTGAGG CCTCAGC(-5/-2)
BclI 2808 TGATCA T^GATCA
BfrI 2722 CTTAAG C^TTAAG
BlnI 1327 CCTAGG C^CTAGG
BsaBI 2018 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3121 CTGCAC GTGCAG(16/14)
BsiEI 4073 CGGCCG CG[GA][CT]^CG
BsmBI 3656 GAGACG CGTCTC(1/5)
BspLU11I 588 ACATGT A^CATGT
BsrDI 4145 CATTGC GCAATG(2/0)
BssHII 3534 GCGCGC G^CGCGC
BssSI 2114 CACGAG CACGAG(-5/-1)
BstBI 3356 TTCGAA TT^CGAA
BstEII 231 GGTGACC G^GT[ACGT]ACC
BstPI 231 GGTGACC G^GT[ACGT]ACC
Bsu36I 1907 CCTTAGG CC^T[ACGT]AGG
EagI 4073 CGGCCG C^GGCCG
EclXI 4073 CGGCCG C^GGCCG
Eco47III 2402 AGCGCT AGC^GCT
Eco52I 4073 CGGCCG C^GGCCG
Eco81I 1907 CCTTAGG CC^T[ACGT]AGG
EcoO65I 231 GGTGACC G^GT[ACGT]ACC
EcoRI 3138 GAATTC G^AATTC
FbaI 2808 TGATCA T^GATCA
KspI 3550 CCGCGG CCGC^GG
MamI 2018 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 827 GCCGGC GCC^GGC
NdeI 2408 CATATG CA^TATG
NgoMIV 827 GCCGGC G^CCGGC
NspV 3356 TTCGAA TT^CGAA
PaeR7I 428 CTCGAG C^TCGAG
PflMI 1575 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4113 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 805 AACGTT AA^CGTT
PvuII 863 CAGCTG CAG^CTG
SacII 3550 CCGCGG CCGC^GG
SalI 4162 GTCGAC G^TCGAC
SapI 2541 GAAGAGC GCTCTTC(1/4)
SfuI 3356 TTCGAA TT^CGAA
SmaI 3543 CCCGGG CCC^GGG
SnaBI 379 TACGTA TAC^GTA
SphI 3266 GCATGC GCATG^C
SspI 987 AATATT AAT^ATT
Van91I 1575 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 428 CTCGAG C^TCGAG
XmaCI 3543 CCCGGG C^CCGGG
XmaI 3543 CCCGGG C^CCGGG
XmnI 2512 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1860 GACGCC G[GA]^CG[CT]C
3186 GACGCC
AflIII 588 ACATGT A^C[GA][CT]GT
600 ACGTGT
AhdI 214 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1135 GACCTGCGGTC
AspEI 214 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1135 GACCTGCGGTC
AspI 1773 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2130 GACACCGTC
AvaI 428 CTCGAG C^[CT]CG[GA]G
3543 CCCGGG
BanI 3079 GGTGCC G^G[CT][GA]CC
3106 GGTGCC
BsaAI 379 TACGTA [CT]AC^GT[GA]
3320 TACGTG
BsaHI 1860 GACGCC G[GA]^CG[CT]C
3186 GACGCC
BsoBI 428 CTCGAG C^[CT]CG[GA]G
3543 CCCGGG
BsrBI 1659 CCGCTC CCGCTC(-3/-3)
3989 GAGCGG
BsrFI 827 GCCGGC [GA]^CCGG[CT]
2051 GCCGGT
BstAPI 30 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1819 GCAGCCAGTGC
Cfr10I 827 GCCGGC [GA]^CCGG[CT]
2051 GCCGGT
DraI 1117 TTTAAA TTT^AAA
3916 TTTAAA
Eam1105I 214 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1135 GACCTGCGGTC
EarI 895 GAAGAG CTCTTC(1/4)
2541 GAAGAG
EcoNI 24 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3615 CCTGAAAGAGG
Hin1I 1860 GACGCC G[GA]^CG[CT]C
3186 GACGCC
HincII 1767 GTCAAC GT[CT]^[GA]AC
4162 GTCGAC
HindII 1767 GTCAAC GT[CT]^[GA]AC
4162 GTCGAC
Ksp632I 895 GAAGAG CTCTTC(1/4)
2541 GAAGAG
NcoI 1212 CCATGG C^CATGG
3246 CCATGG
NspI 588 ACATGT [GA]CATG^[CT]
3266 GCATGC
PflFI 1773 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2130 GACACCGTC
PpuMI 1133 AGGACCT [GA]G^G[AT]CC[CT]
1777 GGGTCCC
SexAI 2244 ACCTGGT A^CC[AT]GGT
3680 ACCTGGT
Tth111I 1773 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2130 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BamHI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII