Restriction map for
pFN21AA0622
(Linear) (Six-base) MAPSORT of: KIAA0622.seq Check: 2036 from: 1 to: 4389
KIAA0622.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 21 4389
Size: 21 4368
AccI GT'mk_AC
Cuts at: 0 4163 4389
Size: 4163 226
AclI AA'CG_TT
Cuts at: 0 806 4389
Size: 806 3583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1241 1533 1639 2123 2560 3320 4389
Size: 845 396 292 106 484 437 760 1069
Fragments arranged by size:
1069 845 760 484 437 396 292 106
AfeI AGC'GCT
Cuts at: 0 2404 4389
Size: 2404 1985
AflII C'TTAA_G
Cuts at: 0 2722 4389
Size: 2722 1667
AflIII A'CryG_T
Cuts at: 0 588 600 4389
Size: 588 12 3789
Fragments arranged by size:
3789 588 12
AhdI GACnn_n'nnGTC
Cuts at: 0 219 1140 4389
Size: 219 921 3249
Fragments arranged by size:
3249 921 219
AleI CACnn'nnGTG
Cuts at: 0 3154 4389
Size: 3154 1235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 292 520 4066 4389
Size: 292 228 3546 323
Fragments arranged by size:
3546 323 292 228
ApaI G_GGCC'C
Cuts at: 0 4338 4389
Size: 4338 51
ApoI r'AATT_y
Cuts at: 0 417 578 664 717 1396 3061 3138 3890
Size: 417 161 86 53 679 1665 77 752
Cuts at: 3890 4389
Size: 499
Fragments arranged by size:
1665 752 679 499 417 161 86 77
53
AvaI C'yCGr_G
Cuts at: 0 428 3543 4389
Size: 428 3115 846
Fragments arranged by size:
3115 846 428
AvrII C'CTAG_G
Cuts at: 0 1327 4389
Size: 1327 3062
BamHI G'GATC_C
Cuts at: 0 850 1916 2919 4389
Size: 850 1066 1003 1470
Fragments arranged by size:
1470 1066 1003 850
BanI G'GyrC_C
Cuts at: 0 3079 3106 4389
Size: 3079 27 1283
Fragments arranged by size:
3079 1283 27
BanII G_rGCy'C
Cuts at: 0 2110 3163 3747 4338 4389
Size: 2110 1053 584 591 51
Fragments arranged by size:
2110 1053 591 584 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 851 2129 3782 3952 4271 4389
Size: 851 1278 1653 170 319 118
Fragments arranged by size:
1653 1278 851 319 170 118
BbvCI CC'TCA_GC
Cuts at: 0 3995 4389
Size: 3995 394
BclI T'GATC_A
Cuts at: 0 2808 4389
Size: 2808 1581
BglII A'GATC_T
Cuts at: 0 612 1045 2023 2286 2588 3369 4389
Size: 612 433 978 263 302 781 1020
Fragments arranged by size:
1020 978 781 612 433 302 263
Bme1580I G_kGCm'C
Cuts at: 0 1858 3665 4338 4389
Size: 1858 1807 673 51
Fragments arranged by size:
1858 1807 673 51
BmgBI CAC'GTC
Cuts at: 0 3006 3660 4389
Size: 3006 654 729
Fragments arranged by size:
3006 729 654
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 1539 1784 2111 4389
Size: 369 1170 245 327 2278
Fragments arranged by size:
2278 1170 369 327 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1768 1874 2400 3772 3850 4389
Size: 494 1274 106 526 1372 78 539
Fragments arranged by size:
1372 1274 539 526 494 106 78
Bpu10I CC'TnA_GC
Cuts at: 0 1130 3995 4389
Size: 1130 2865 394
Fragments arranged by size:
2865 1130 394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 979 1661 2370 2851 3118 3745 4389
Size: 139 840 682 709 481 267 627 644
Fragments arranged by size:
840 709 682 644 627 481 267 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 947 1148 3826 4389
Size: 947 201 2678 563
Fragments arranged by size:
2678 947 563 201
BsaAI yAC'GTr
Cuts at: 0 381 3322 4389
Size: 381 2941 1067
Fragments arranged by size:
2941 1067 381
BsaBI GATnn'nnATC
Cuts at: 0 2022 4389
Size: 2022 2367
BsaHI Gr'CG_yC
Cuts at: 0 1861 3187 4389
Size: 1861 1326 1202
Fragments arranged by size:
1861 1326 1202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 587 617 2841 2871 3513 3543 3952 3982
Size: 587 30 2224 30 642 30 409 30
Cuts at: 3982 3991 4021 4345 4375 4389
Size: 9 30 324 30 14
Fragments arranged by size:
2224 642 587 409 324 30 30 30
30 30 30 14 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1833 2100 2343 2346 3811 4012 4353 4356
Size: 1833 267 243 3 1465 201 341 3
Cuts at: 4356 4389
Size: 33
Fragments arranged by size:
1833 1465 341 267 243 201 33 3
3
BseYI C'CCAG_C
Cuts at: 0 204 2012 3524 4389
Size: 204 1808 1512 865
Fragments arranged by size:
1808 1512 865 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3106 4389
Size: 3106 1283
BsiEI CG_ry'CG
Cuts at: 0 4076 4389
Size: 4076 313
BsiHKAI G_wGCw'C
Cuts at: 0 480 1568 2278 3803 3867 4055 4389
Size: 480 1088 710 1525 64 188 334
Fragments arranged by size:
1525 1088 710 480 334 188 64
BsmI GAATG_Cn'
Cuts at: 0 398 2262 2429 4389
Size: 398 1864 167 1960
Fragments arranged by size:
1960 1864 398 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3650 4389
Size: 3650 739
Bsp1286I G_dGCh'C
Cuts at: 0 480 1568 1858 2110 2278 3163 3665 3747
Size: 480 1088 290 252 168 885 502 82
Cuts at: 3747 3803 3867 4055 4338 4389
Size: 56 64 188 283 51
Fragments arranged by size:
1088 885 502 480 290 283 252 188
168 82 64 56 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 54 1145 4389
Size: 21 33 1091 3244
Fragments arranged by size:
3244 1091 33 21
BsrBI CCG'CTC
Cuts at: 0 1661 3991 4389
Size: 1661 2330 398
Fragments arranged by size:
2330 1661 398
BsrDI GCAATG_nn'
Cuts at: 0 4144 4389
Size: 4144 245
BsrFI r'CCGG_y
Cuts at: 0 827 2051 4389
Size: 827 1224 2338
Fragments arranged by size:
2338 1224 827
BssHII G'CGCG_C
Cuts at: 0 3534 4389
Size: 3534 855
BssSI C'ACGA_G
Cuts at: 0 2114 4389
Size: 2114 2275
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 1825 4389
Size: 36 1789 2564
Fragments arranged by size:
2564 1789 36
BstBI TT'CG_AA
Cuts at: 0 3357 4389
Size: 3357 1032
BstEII G'GTnAC_C
Cuts at: 0 231 4389
Size: 231 4158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 1272 2916 4389
Size: 685 587 1644 1473
Fragments arranged by size:
1644 1473 685 587
BstYI r'GATC_y
Cuts at: 0 531 612 850 1045 1916 2023 2286 2588
Size: 531 81 238 195 871 107 263 302
Cuts at: 2588 2749 2919 3369 4389
Size: 161 170 450 1020
Fragments arranged by size:
1020 871 531 450 302 263 238 195
170 161 107 81
Bsu36I CC'TnA_GG
Cuts at: 0 1908 4389
Size: 1908 2481
BtgI C'CryG_G
Cuts at: 0 1212 3246 3550 4389
Size: 1212 2034 304 839
Fragments arranged by size:
2034 1212 839 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3440 4389
Size: 3440 949
BtsI GCAGTG_nn'
Cuts at: 0 4045 4389
Size: 4045 344
DraI TTT'AAA
Cuts at: 0 1119 3918 4389
Size: 1119 2799 471
Fragments arranged by size:
2799 1119 471
EaeI y'GGCC_r
Cuts at: 0 69 1274 2226 2906 3180 4016 4073 4389
Size: 69 1205 952 680 274 836 57 316
Fragments arranged by size:
1205 952 836 680 316 274 69 57
EagI C'GGCC_G
Cuts at: 0 4073 4389
Size: 4073 316
EarI CTCTTCn'nnn_
Cuts at: 0 890 2536 4389
Size: 890 1646 1853
Fragments arranged by size:
1853 1646 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 213 4389
Size: 213 4176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 845 1241 1533 1639 1768 1874 2123
Size: 494 351 396 292 106 129 106 249
Cuts at: 2123 2400 2560 3320 3772 3850 4389
Size: 277 160 760 452 78 539
Fragments arranged by size:
760 539 494 452 396 351 292 277
249 160 129 106 106 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 3619 4389
Size: 28 3591 770
Fragments arranged by size:
3591 770 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1134 1778 4334 4389
Size: 1134 644 2556 55
Fragments arranged by size:
2556 1134 644 55
EcoRI G'AATT_C
Cuts at: 0 3138 4389
Size: 3138 1251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2533 2713 3799 4389
Size: 2533 180 1086 590
Fragments arranged by size:
2533 1086 590 180
HaeII r_GCGC'y
Cuts at: 0 1389 2406 3437 4389
Size: 1389 1017 1031 952
Fragments arranged by size:
1389 1031 1017 952
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 205 237 342 374 422 454
Size: 76 32 97 32 105 32 48 32
Cuts at: 454 597 629 1126 1158 1648 1674 1680 1706
Size: 143 32 497 32 490 26 6 26
Cuts at: 1706 2580 2612 3403 3435 3991 4023 4389
Size: 874 32 791 32 556 32 366
Fragments arranged by size:
874 791 556 497 490 366 143 105
97 76 48 32 32 32 32 32
32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 0 1769 4164 4389
Size: 1769 2395 225
Fragments arranged by size:
2395 1769 225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1894 2947 3734 4389
Size: 789 1105 1053 787 655
Fragments arranged by size:
1105 1053 789 787 655
MscI TGG'CCA
Cuts at: 0 71 1276 2228 2908 4018 4389
Size: 71 1205 952 680 1110 371
Fragments arranged by size:
1205 1110 952 680 371 71
MslI CAynn'nnrTG
Cuts at: 0 513 2599 2678 2827 3154 4048 4105 4389
Size: 513 2086 79 149 327 894 57 284
Fragments arranged by size:
2086 894 513 327 284 149 79 57
MspA1I CmG'CkG
Cuts at: 0 865 1698 3552 4389
Size: 865 833 1854 837
Fragments arranged by size:
1854 865 837 833
NaeI GCC'GGC
Cuts at: 0 829 4389
Size: 829 3560
NcoI C'CATG_G
Cuts at: 0 1212 3246 4389
Size: 1212 2034 1143
Fragments arranged by size:
2034 1212 1143
NdeI CA'TA_TG
Cuts at: 0 2409 4389
Size: 2409 1980
NgoMIV G'CCGG_C
Cuts at: 0 827 4389
Size: 827 3562
NspI r_CATG'y
Cuts at: 0 592 3270 4389
Size: 592 2678 1119
Fragments arranged by size:
2678 1119 592
PciI A'CATG_T
Cuts at: 0 588 4389
Size: 588 3801
PflMI CCAn_nnn'nTGG
Cuts at: 0 1581 4389
Size: 1581 2808
PfoI T'CCnGG_A
Cuts at: 0 2416 3098 4389
Size: 2416 682 1291
Fragments arranged by size:
2416 1291 682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3280 3312 3613 3645 4389
Size: 3280 32 301 32 744
Fragments arranged by size:
3280 744 301 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1134 1778 4389
Size: 1134 644 2611
Fragments arranged by size:
2611 1134 644
PshAI GACnn'nnGTC
Cuts at: 0 4117 4389
Size: 4117 272
PsiI TTA'TAA
Cuts at: 0 2665 4389
Size: 2665 1724
PspOMI G'GGCC_C
Cuts at: 0 4334 4389
Size: 4334 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2453 2485 4389
Size: 2453 32 1904
Fragments arranged by size:
2453 1904 32
PstI C_TGCA'G
Cuts at: 0 245 1610 1878 2531 4154 4187 4389
Size: 245 1365 268 653 1623 33 202
Fragments arranged by size:
1623 1365 653 268 245 202 33
PvuII CAG'CTG
Cuts at: 0 865 4389
Size: 865 3524
SacII CC_GC'GG
Cuts at: 0 3553 4389
Size: 3553 836
SalI G'TCGA_C
Cuts at: 0 4162 4389
Size: 4162 227
SanDI GG'GwC_CC
Cuts at: 0 1778 4389
Size: 1778 2611
SapI GCTCTTCn'nnn_
Cuts at: 0 2536 4389
Size: 2536 1853
SexAI A'CCwGG_T
Cuts at: 0 2244 3680 4389
Size: 2244 1436 709
Fragments arranged by size:
2244 1436 709
SfcI C'TryA_G
Cuts at: 0 241 929 1606 1745 1874 2527 3309 4150
Size: 241 688 677 139 129 653 782 841
Cuts at: 4150 4183 4389
Size: 33 206
Fragments arranged by size:
841 782 688 677 653 241 206 139
129 33
SmaI CCC'GGG
Cuts at: 0 3545 4389
Size: 3545 844
SmlI C'TyrA_G
Cuts at: 0 118 428 958 1676 2385 2722 2866 3133
Size: 118 310 530 718 709 337 144 267
Cuts at: 3133 3760 4389
Size: 627 629
Fragments arranged by size:
718 709 629 627 530 337 310 267
144 118
SnaBI TAC'GTA
Cuts at: 0 381 4389
Size: 381 4008
SphI G_CATG'C
Cuts at: 0 3270 4389
Size: 3270 1119
SspI AAT'ATT
Cuts at: 0 989 4389
Size: 989 3400
StyI C'CwwG_G
Cuts at: 0 779 846 1212 1327 1580 3246 3783 3837
Size: 779 67 366 115 253 1666 537 54
Cuts at: 3837 4389
Size: 552
Fragments arranged by size:
1666 779 552 537 366 253 115 67
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 3246 3538 4389
Size: 162 3084 292 851
Fragments arranged by size:
3084 851 292 162
TatI w'GTAC_w
Cuts at: 0 933 2281 4389
Size: 933 1348 2108
Fragments arranged by size:
2108 1348 933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 3513 3545 3952 3984 4389
Size: 587 32 2894 32 407 32 405
Fragments arranged by size:
2894 587 407 405 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1776 2133 4389
Size: 1776 357 2256
Fragments arranged by size:
2256 1776 357
XbaI T'CTAG_A
Cuts at: 0 1735 2778 2965 4389
Size: 1735 1043 187 1424
Fragments arranged by size:
1735 1424 1043 187
XhoI C'TCGA_G
Cuts at: 0 428 4389
Size: 428 3961
XmaI C'CCGG_G
Cuts at: 0 3543 4389
Size: 3543 846
XmnI GAAnn'nnTTC
Cuts at: 0 2516 4389
Size: 2516 1873
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BclI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII
SacII SalI SanDI SapI SexAI SfcI SmaI SmlI
SnaBI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI AloI AlwNI ApaLI AscI AseI
AsiSI BaeI BbeI BcgI BciVI BfrBI BglI BlpI
BmtI BplI BsaWI BsiWI BspEI BspHI BsrGI BstZ17I
ClaI CspCI DraIII DrdI EcoICRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PspXI PvuI RsrII SacI SbfI ScaI SfiI SfoI
SgfI SgrAI SpeI SrfI StuI SwaI XcmI ZraI