(Circular) (Six-base) MAPSORT of: pFN21AA0611.seq Check: 6492 from: 1 to: 7438 pFN21AA0611 7438 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3966 5248 278 Size: 53 83 186 936 2430 1282 2468 Fragments arranged by size: 2468 2430 1282 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3078 4781 1937 Size: 436 705 1703 4594 Fragments arranged by size: 4594 1703 705 436 Acc65I G'GTAC_C Cuts at: 2580 4759 2580 Size: 2179 5259 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2543 3896 5501 6885 7153 1632 Size: 911 1353 1605 1384 268 1917 Fragments arranged by size: 1917 1605 1384 1353 911 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 6337 7314 1203 Size: 5134 977 1327 Fragments arranged by size: 5134 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 6167 6167 Size: 7438 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2978 3275 3786 1184 Size: 1794 297 511 4836 Fragments arranged by size: 4836 1794 511 297 AlwNI CAG_nnn'CTG Cuts at: 3196 5181 6753 3196 Size: 1985 1572 3881 Fragments arranged by size: 3881 1985 1572 ApaI G_GGCC'C Cuts at: 2921 2921 Size: 7438 ApaLI G'TGCA_C Cuts at: 2259 2963 3470 3652 4448 5495 6651 2259 Size: 704 507 182 796 1047 1156 3046 Fragments arranged by size: 3046 1156 1047 796 704 507 182 ApoI r'AATT_y Cuts at: 1493 4747 4973 5125 7234 1493 Size: 3254 226 152 2109 1697 Fragments arranged by size: 3254 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2702 3372 3737 3784 4763 1870 Size: 73 153 606 670 365 47 979 4545 Fragments arranged by size: 4545 979 670 606 365 153 73 47 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2388 2421 2388 Size: 33 7405 BamHI G'GATC_C Cuts at: 4768 5135 4768 Size: 367 7071 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2522 2580 4759 6214 Size: 325 217 627 304 431 58 2179 1455 Cuts at: 6214 618 Size: 1842 Fragments arranged by size: 2179 1842 1455 627 431 325 304 217 58 BanII G_rGCy'C Cuts at: 1391 2921 4323 4757 1391 Size: 1530 1402 434 4072 Fragments arranged by size: 4072 1530 1402 434 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3069 3153 4705 928 Size: 971 1170 84 1552 3661 Fragments arranged by size: 3661 1552 1170 971 84 BbvCI CC'TCA_GC Cuts at: 3042 3173 4121 3042 Size: 131 948 6359 Fragments arranged by size: 6359 948 131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5629 5663 1903 Size: 34 3692 34 3678 Fragments arranged by size: 3692 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2127 4323 5330 6540 2127 Size: 2196 1007 1210 3025 Fragments arranged by size: 3025 2196 1210 1007 BclI T'GATC_A Cuts at: 1548 3034 1548 Size: 1486 5952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2978 3543 6049 Size: 107 122 71 631 605 1306 565 2506 Cuts at: 6049 136 Size: 1525 Fragments arranged by size: 2506 1525 1306 631 605 565 122 107 71 BglII A'GATC_T Cuts at: 3216 7433 3216 Size: 4217 3221 BlpI GC'TnA_GC Cuts at: 4553 4855 4553 Size: 302 7136 Bme1580I G_kGCm'C Cuts at: 1790 2094 2263 2527 2921 2967 3474 3656 3775 Size: 304 169 264 394 46 507 182 119 Cuts at: 3775 3854 3880 4452 5499 6655 1790 Size: 79 26 572 1047 1156 2573 Fragments arranged by size: 2573 1156 1047 572 507 394 304 264 182 169 119 79 46 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 5169 6127 Size: 416 53 83 399 24 867 2885 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 2885 1753 958 867 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2136 2136 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2704 3297 3533 3605 3659 3818 6098 1239 Size: 1465 593 236 72 54 159 2280 2579 Fragments arranged by size: 2579 2280 1465 593 236 159 72 54 Bpu10I CC'TnA_GC Cuts at: 2733 3042 3173 4121 4317 2733 Size: 309 131 948 196 5854 Fragments arranged by size: 5854 948 309 196 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3794 4322 4670 5563 6428 6726 3794 Size: 528 348 893 865 298 4506 Fragments arranged by size: 4506 893 865 528 348 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2100 2542 3147 3581 6101 882 Size: 617 601 442 605 434 2520 2219 Fragments arranged by size: 2520 2219 617 605 601 442 434 BsaAI yAC'GTr Cuts at: 493 1204 2344 3651 5229 493 Size: 711 1140 1307 1578 2702 Fragments arranged by size: 2702 1578 1307 1140 711 BsaBI GATnn'nnATC Cuts at: 4388 7432 4388 Size: 3044 4394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3963 5245 5627 6262 Size: 53 83 186 936 2430 1282 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2430 1451 1282 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2436 5871 6269 6543 6690 7101 2436 Size: 3435 398 274 147 411 2773 Fragments arranged by size: 3435 2773 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3080 3294 3324 3341 Size: 30 165 30 1647 30 214 30 17 Cuts at: 3341 3371 3464 3494 4135 4165 4181 4211 4559 Size: 30 93 30 641 30 16 30 348 Cuts at: 4559 4589 1178 Size: 30 4027 Fragments arranged by size: 4027 1647 641 348 214 165 93 30 30 30 30 30 30 30 30 30 17 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2594 3434 1186 Size: 1156 252 840 5190 Fragments arranged by size: 5190 1156 840 252 BseYI C'CCAG_C Cuts at: 2086 2673 2740 2886 4715 6641 7137 2086 Size: 587 67 146 1829 1926 496 2387 Fragments arranged by size: 2387 1926 1829 587 496 146 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3350 3960 1163 Size: 573 1614 610 4641 Fragments arranged by size: 4641 1614 610 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 3858 4349 4888 5649 6677 Size: 275 186 1477 380 491 539 761 1028 Cuts at: 6677 1540 Size: 2301 Fragments arranged by size: 2301 1477 1028 761 539 491 380 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 2967 3393 3474 3656 4323 4406 4452 5499 Size: 704 426 81 182 667 83 46 1047 Cuts at: 5499 5584 6655 2263 Size: 85 1071 3046 Fragments arranged by size: 3046 1071 1047 704 667 426 182 85 83 81 46 BsmI GAATG_Cn' Cuts at: 3604 3670 4949 5042 3604 Size: 66 1279 93 6000 Fragments arranged by size: 6000 1279 93 66 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2263 2527 2921 2967 3393 3474 Size: 399 304 169 264 394 46 426 81 Cuts at: 3474 3656 3775 3854 3880 4323 4406 4452 4757 Size: 182 119 79 26 443 83 46 305 Cuts at: 4757 5499 5584 6655 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 426 399 394 305 304 264 182 169 119 85 83 81 79 46 46 26 BspEI T'CCGG_A Cuts at: 2436 7101 2436 Size: 4665 2773 BspHI T'CATG_A Cuts at: 2445 4533 5327 7057 2445 Size: 2088 794 1730 2826 Fragments arranged by size: 2826 2088 1730 794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2592 4701 4793 844 Size: 1748 2109 92 3489 Fragments arranged by size: 3489 2109 1748 92 BsrBI CCG'CTC Cuts at: 1394 1823 3480 5325 1394 Size: 429 1657 1845 3507 Fragments arranged by size: 3507 1845 1657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3964 5933 6107 65 Size: 1183 2716 1969 174 1396 Fragments arranged by size: 2716 1969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 2905 4071 6082 6269 1700 Size: 110 569 526 1166 2011 187 2869 Fragments arranged by size: 2869 2011 1166 569 526 187 110 BsrGI T'GTAC_A Cuts at: 96 2667 96 Size: 2571 4867 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 2391 3392 4592 5498 6510 2391 Size: 1001 1200 906 1012 3319 Fragments arranged by size: 3319 1200 1012 1001 906 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7438 BstBI TT'CG_AA Cuts at: 2683 6242 2683 Size: 3559 3879 BstEII G'GTnAC_C Cuts at: 1184 3676 3712 1184 Size: 2492 36 4910 Fragments arranged by size: 4910 2492 36 BstYI r'GATC_y Cuts at: 1972 2331 2511 3192 3216 4330 4768 5135 5520 Size: 359 180 681 24 1114 438 367 385 Cuts at: 5520 5537 6989 7075 7087 7433 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1114 681 438 385 367 359 346 180 86 24 17 12 BstZ17I GTA'TAC Cuts at: 3079 3079 Size: 7438 Bsu36I CC'TnA_GG Cuts at: 3593 3593 Size: 7438 BtgI C'CryG_G Cuts at: 513 1065 2004 2709 3495 3721 513 Size: 552 939 705 786 226 4230 Fragments arranged by size: 4230 939 786 705 552 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2968 4555 4600 487 Size: 683 56 1742 1587 45 3325 Fragments arranged by size: 3325 1742 1587 683 56 45 BtsI GCAGTG_nn' Cuts at: 4954 5748 5768 4954 Size: 794 20 6624 Fragments arranged by size: 6624 794 20 ClaI AT'CG_AT Cuts at: 1554 4602 1554 Size: 3048 4390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DraIII CAC_nnn'GTG Cuts at: 3001 4456 3001 Size: 1455 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2357 4286 6445 809 Size: 507 1041 1929 2159 1802 Fragments arranged by size: 2159 1929 1802 1041 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 3492 3855 Size: 54 1425 50 275 18 468 1194 363 Cuts at: 3855 4069 4090 4346 4458 4885 5774 7403 8 Size: 214 21 256 112 427 889 1629 43 Fragments arranged by size: 1629 1425 1194 889 468 427 363 275 256 214 112 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3855 4346 4885 1537 Size: 275 2043 491 539 4090 Fragments arranged by size: 4090 2043 539 491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 3348 5368 1353 Size: 743 1252 2020 3423 Fragments arranged by size: 3423 2020 1252 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2959 4559 6025 6399 6545 387 Size: 1064 138 1370 1600 1466 374 146 1280 Fragments arranged by size: 1600 1466 1370 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4321 4321 Size: 7438 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 2704 3297 3533 3605 3659 3818 Size: 393 911 161 593 236 72 54 159 Cuts at: 3818 3896 5501 6098 6885 7153 1239 Size: 78 1605 597 787 268 1524 Fragments arranged by size: 1605 1524 911 787 597 593 393 268 236 161 159 78 72 54 EcoNI CCTnn'n_nnAGG Cuts at: 3754 3911 3754 Size: 157 7281 EcoO109I rG'GnC_Cy Cuts at: 2016 2070 2899 2917 3177 2016 Size: 54 829 18 260 6277 Fragments arranged by size: 6277 829 260 54 18 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2533 4384 2533 Size: 1851 5587 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 4424 4508 6585 1678 Size: 2746 84 2077 2531 Fragments arranged by size: 2746 2531 2077 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2764 2796 4209 4241 4277 Size: 32 135 32 1192 32 1413 32 36 Cuts at: 4277 4309 6079 6111 6153 6185 1373 Size: 32 1770 32 42 32 2626 Fragments arranged by size: 2626 1770 1413 1192 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 4782 5028 669 Size: 988 281 424 2420 246 3079 Fragments arranged by size: 3079 2420 988 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7438 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 2584 4763 2584 Size: 2179 5259 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2640 3045 3327 4419 6552 6736 1305 Size: 1335 405 282 1092 2133 184 2007 Fragments arranged by size: 2133 2007 1335 1092 405 282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3494 4460 10 Size: 54 1425 343 1662 966 2988 Fragments arranged by size: 2988 1662 1425 966 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 3768 4409 4538 4604 4633 5396 Size: 648 2091 511 641 129 66 29 763 Cuts at: 5396 5755 5914 518 Size: 359 159 2042 Fragments arranged by size: 2091 2042 763 648 641 511 359 159 129 66 29 MspA1I CmG'CkG Cuts at: 1606 2673 2740 3172 3559 3723 4665 4854 5531 Size: 1067 67 432 387 164 942 189 677 Cuts at: 5531 6679 6924 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1067 942 677 432 387 245 189 164 67 NaeI GCC'GGC Cuts at: 2381 2381 Size: 7438 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5947 Fragments arranged by size: 5947 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NgoMIV G'CCGG_C Cuts at: 2379 2379 Size: 7438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 4796 6341 1132 Size: 97 3567 1545 2229 Fragments arranged by size: 3567 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2284 1348 Size: 936 6502 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7438 PfoI T'CCnGG_A Cuts at: 4275 4275 Size: 7438 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 4427 4459 5507 5539 7020 7052 3812 Size: 32 583 32 1048 32 1481 32 4198 Fragments arranged by size: 4198 1481 1048 583 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2899 3177 2899 Size: 278 7160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PspOMI G'GGCC_C Cuts at: 2917 2917 Size: 7438 PspXI vC'TCGA_Gb Cuts at: 3737 3737 Size: 7438 PstI C_TGCA'G Cuts at: 830 2589 3564 4039 4790 830 Size: 1759 975 475 751 3478 Fragments arranged by size: 3478 1759 975 751 475 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 2673 2740 3172 3559 4665 2673 Size: 67 432 387 1106 5446 Fragments arranged by size: 5446 1106 432 387 67 SacI G_AGCT'C Cuts at: 4323 4323 Size: 7438 SacII CC_GC'GG Cuts at: 3724 3724 Size: 7438 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 3815 5686 7398 3815 Size: 1871 1712 3855 Fragments arranged by size: 3855 1871 1712 SfcI C'TryA_G Cuts at: 826 1045 2374 2585 3212 3560 4035 4786 5921 Size: 219 1329 211 627 348 475 751 1135 Cuts at: 5921 6602 6793 826 Size: 681 191 1471 Fragments arranged by size: 1471 1329 1135 751 681 627 475 348 219 211 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 2704 4765 1872 Size: 832 2061 4545 Fragments arranged by size: 4545 2061 832 SmlI C'TyrA_G Cuts at: 820 1017 1943 3737 3809 4337 4685 5542 6443 Size: 197 926 1794 72 528 348 857 901 Cuts at: 6443 6705 820 Size: 262 1553 Fragments arranged by size: 1794 1553 926 901 857 528 348 262 197 72 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7438 StyI C'CwwG_G Cuts at: 513 1065 2004 4877 513 Size: 552 939 2873 3074 Fragments arranged by size: 3074 2873 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2396 2665 2900 3899 5465 5482 5635 Size: 1255 816 269 235 999 1566 17 153 Cuts at: 5635 5820 6265 325 Size: 185 445 1498 Fragments arranged by size: 1566 1498 1255 999 816 445 269 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2667 2857 Size: 274 80 33 51 1443 168 522 190 Cuts at: 2857 3333 3813 4608 5684 7396 96 Size: 476 480 795 1076 1712 138 Fragments arranged by size: 1712 1443 1076 795 522 480 476 274 190 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2149 2181 3112 3144 3294 3326 3341 3373 3464 Size: 32 931 32 150 32 15 32 91 Cuts at: 3464 3496 3518 3550 4240 4272 7398 7430 2149 Size: 32 22 32 690 32 3126 32 2157 Fragments arranged by size: 3126 2157 931 690 150 91 32 32 32 32 32 32 32 32 22 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7438 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XhoI C'TCGA_G Cuts at: 1943 3737 1943 Size: 1794 5644 XmaI C'CCGG_G Cuts at: 1870 2702 4763 1870 Size: 832 2061 4545 Fragments arranged by size: 4545 2061 832 XmnI GAAnn'nnTTC Cuts at: 3749 5567 3749 Size: 1818 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3964 5246 276 Size: 53 83 186 936 2430 1282 2468 Fragments arranged by size: 2468 2430 1282 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI