(Circular) (Six-base) MAPSORT of: pFN21AA0609.seq Check: 5387 from: 1 to: 6817 pFN21AA0609 6817 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3270 4627 278 Size: 53 83 186 936 1734 1357 2468 Fragments arranged by size: 2468 1734 1357 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4160 1937 Size: 2223 4594 Acc65I G'GTAC_C Cuts at: 2832 3726 4138 2832 Size: 894 412 5511 Fragments arranged by size: 5511 894 412 AclI AA'CG_TT Cuts at: 1563 4944 5317 1563 Size: 3381 373 3063 Fragments arranged by size: 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 4880 6264 6532 1632 Size: 1011 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 1011 268 AfeI AGC'GCT Cuts at: 3197 3197 Size: 6817 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6620 AflIII A'CryG_T Cuts at: 1203 3120 3522 4059 5716 6693 1203 Size: 1917 402 537 1657 977 1327 Fragments arranged by size: 1917 1657 1327 977 537 402 AgeI A'CCGG_T Cuts at: 5648 5648 Size: 6817 AhdI GACnn_n'nnGTC Cuts at: 5546 5546 Size: 6817 AleI CACnn'nnGTG Cuts at: 1166 2897 1166 Size: 1731 5086 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6817 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3449 3481 3914 3946 3449 Size: 32 433 32 6320 Fragments arranged by size: 6320 433 32 32 AlwNI CAG_nnn'CTG Cuts at: 2548 3310 4560 6132 2548 Size: 762 1250 1572 3233 Fragments arranged by size: 3233 1572 1250 762 ApaI G_GGCC'C Cuts at: 3342 3342 Size: 6817 ApaLI G'TGCA_C Cuts at: 4874 6030 4874 Size: 1156 5661 ApoI r'AATT_y Cuts at: 1493 2033 4126 4352 4504 6613 1493 Size: 540 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 540 226 152 AseI AT'TA_AT Cuts at: 160 1032 5371 160 Size: 872 4339 1606 Fragments arranged by size: 4339 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 AvaI C'yCGr_G Cuts at: 1870 1943 4142 1870 Size: 73 2199 4545 Fragments arranged by size: 4545 2199 73 BamHI G'GATC_C Cuts at: 2634 4147 4514 2634 Size: 1513 367 4937 Fragments arranged by size: 4937 1513 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 2832 3726 4138 5593 Size: 325 217 627 788 257 894 412 1455 Cuts at: 5593 618 Size: 1842 Fragments arranged by size: 1842 1455 894 788 627 412 325 257 217 BanII G_rGCy'C Cuts at: 1391 2275 2616 2976 3342 3407 3564 4136 1391 Size: 884 341 360 366 65 157 572 4072 Fragments arranged by size: 4072 884 572 366 360 341 157 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 928 Size: 971 149 5697 Fragments arranged by size: 5697 971 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5008 5042 1903 Size: 34 50 34 2987 34 3678 Fragments arranged by size: 3678 2987 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2435 4709 5919 2435 Size: 2274 1210 3333 Fragments arranged by size: 3333 2274 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6817 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4074 5428 136 Size: 107 122 71 631 605 2402 1354 1525 Fragments arranged by size: 2402 1525 1354 631 605 122 107 71 BglII A'GATC_T Cuts at: 6812 6812 Size: 6817 BlpI GC'TnA_GC Cuts at: 2252 3282 4234 2252 Size: 1030 952 4835 Fragments arranged by size: 4835 1030 952 Bme1580I G_kGCm'C Cuts at: 1790 2327 2580 3342 4878 6034 1790 Size: 537 253 762 1536 1156 2573 Fragments arranged by size: 2573 1536 1156 762 537 253 BmgBI CAC'GTC Cuts at: 1318 3121 3733 3842 1318 Size: 1803 612 109 4293 Fragments arranged by size: 4293 1803 612 109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3340 4548 5506 Size: 416 53 83 399 24 1923 1208 958 Cuts at: 5506 442 Size: 1753 Fragments arranged by size: 1923 1753 1208 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6817 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 2159 2219 2349 2729 3122 3287 3332 Size: 919 1 60 130 380 393 165 45 Cuts at: 3332 5477 1239 Size: 2145 2579 Fragments arranged by size: 2579 2145 919 393 380 165 130 60 45 1 Bpu10I CC'TnA_GC Cuts at: 2998 2998 Size: 6817 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2600 4022 4942 5807 6105 2600 Size: 1422 920 865 298 3312 Fragments arranged by size: 3312 1422 920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5480 882 Size: 617 3981 2219 Fragments arranged by size: 3981 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4608 493 Size: 711 3404 2702 Fragments arranged by size: 3404 2702 711 BsaBI GATnn'nnATC Cuts at: 6811 6811 Size: 6817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3267 3369 4624 5006 Size: 53 83 186 936 1734 102 1255 382 Cuts at: 5006 5641 275 Size: 635 1451 Fragments arranged by size: 1734 1451 1255 936 635 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 3861 5250 5648 5922 6069 6480 3861 Size: 1389 398 274 147 411 4198 Fragments arranged by size: 4198 1389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 2933 2963 3083 Size: 30 165 30 753 30 747 30 120 Cuts at: 3083 3113 3449 3479 3674 3704 3914 3944 1178 Size: 30 336 30 195 30 210 30 4051 Fragments arranged by size: 4051 753 747 336 210 195 165 120 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2240 2333 1186 Size: 1051 3 93 5670 Fragments arranged by size: 5670 1051 93 3 BseYI C'CCAG_C Cuts at: 2008 2068 3378 3977 6020 6516 2008 Size: 60 1310 599 2043 496 2309 Fragments arranged by size: 2309 2043 1310 599 496 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 3098 3215 3938 1163 Size: 573 756 606 117 723 4042 Fragments arranged by size: 4042 756 723 606 573 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2397 3862 4267 5028 6056 1540 Size: 275 186 396 1465 405 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 405 396 275 186 BsiHKAI G_wGCw'C Cuts at: 2616 2976 4878 4963 6034 2616 Size: 360 1902 85 1071 3399 Fragments arranged by size: 3399 1902 1071 360 85 BsmI GAATG_Cn' Cuts at: 4328 4421 4328 Size: 93 6724 BsmBI CGTCTCn'nnnn_ Cuts at: 2020 3111 3832 2020 Size: 1091 721 5005 Fragments arranged by size: 5005 1091 721 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2327 2580 2616 2976 3342 3407 Size: 399 485 52 253 36 360 366 65 Cuts at: 3407 3564 4136 4878 4963 6034 1391 Size: 157 572 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 572 485 399 366 360 253 157 85 65 52 36 BspEI T'CCGG_A Cuts at: 6480 6480 Size: 6817 BspHI T'CATG_A Cuts at: 4706 6436 4706 Size: 1730 5087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3489 4172 844 Size: 2645 683 3489 Fragments arranged by size: 3489 2645 683 BsrBI CCG'CTC Cuts at: 1394 1823 3099 4704 1394 Size: 429 1276 1605 3507 Fragments arranged by size: 3507 1605 1276 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 2754 3627 5312 5486 65 Size: 1183 1284 222 873 1685 174 1396 Fragments arranged by size: 1685 1396 1284 1183 873 222 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2392 3654 5461 5648 1700 Size: 110 582 1262 1807 187 2869 Fragments arranged by size: 2869 1807 1262 582 187 110 BsrGI T'GTAC_A Cuts at: 96 3450 96 Size: 3354 3463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6817 BssSI C'ACGA_G Cuts at: 4877 5889 4877 Size: 1012 5805 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6817 BstBI TT'CG_AA Cuts at: 2106 5621 2106 Size: 3515 3302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6817 BstXI CCAn_nnnn'nTGG Cuts at: 3349 3349 Size: 6817 BstYI r'GATC_y Cuts at: 1972 2544 2634 4147 4514 4899 4916 6368 6454 Size: 572 90 1513 367 385 17 1452 86 Cuts at: 6454 6466 6812 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1513 1452 572 385 367 346 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3575 3575 Size: 6817 BtgI C'CryG_G Cuts at: 513 1065 2313 2864 3291 513 Size: 552 1248 551 427 4039 Fragments arranged by size: 4039 1248 552 551 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2267 487 Size: 683 56 1041 5037 Fragments arranged by size: 5037 1041 683 56 BtsI GCAGTG_nn' Cuts at: 3113 3644 4333 5127 5147 3113 Size: 531 689 794 20 4783 Fragments arranged by size: 4783 794 689 531 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2737 2772 529 Size: 35 2173 35 4574 Fragments arranged by size: 4574 2173 35 35 DraI TTT'AAA Cuts at: 4121 4121 Size: 6817 DraIII CAC_nnn'GTG Cuts at: 3433 3448 3433 Size: 15 6802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5824 809 Size: 507 4508 1802 Fragments arranged by size: 4508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2550 2867 3495 Size: 54 1425 50 275 18 720 317 628 Cuts at: 3495 4264 5153 6782 8 Size: 769 889 1629 43 Fragments arranged by size: 1629 1425 889 769 720 628 317 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4264 1537 Size: 275 2452 4090 Fragments arranged by size: 4090 2452 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 2700 2875 3719 4038 4747 1353 Size: 1211 136 175 844 319 709 3423 Fragments arranged by size: 3423 1211 844 709 319 175 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3790 5404 5778 5924 387 Size: 1064 138 2201 1614 374 146 1280 Fragments arranged by size: 2201 1614 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2614 2974 2614 Size: 360 6457 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2159 2219 2349 2643 2729 3122 Size: 393 526 1 60 130 294 86 393 Cuts at: 3122 3287 3332 4880 5477 6264 6532 1239 Size: 165 45 1548 597 787 268 1524 Fragments arranged by size: 1548 1524 787 597 526 393 393 294 268 165 130 86 60 45 1 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2261 Size: 6817 EcoO109I rG'GnC_Cy Cuts at: 3338 3708 3338 Size: 370 6447 EcoRI G'AATT_C Cuts at: 4126 4504 4126 Size: 378 6439 EcoRV GAT'ATC Cuts at: 2737 2737 Size: 6817 FspI TGC'GCA Cuts at: 2207 3488 5323 2207 Size: 1281 1835 3701 Fragments arranged by size: 3701 1835 1281 FspAI rTGC'GCAy Cuts at: 2207 2207 Size: 6817 HaeII r_GCGC'y Cuts at: 1678 3199 5964 1678 Size: 1521 2765 2531 Fragments arranged by size: 2765 2531 1521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 2650 2677 2682 Size: 32 135 32 1031 32 15 27 5 Cuts at: 2682 2706 2709 2738 3674 3706 5458 5490 5532 Size: 24 3 29 936 32 1752 32 42 Cuts at: 5532 5564 1373 Size: 32 2626 Fragments arranged by size: 2626 1752 1031 936 135 42 32 32 32 32 32 32 29 27 24 15 5 3 HincII GTy'rAC Cuts at: 669 1657 1938 4161 4407 669 Size: 988 281 2223 246 3079 Fragments arranged by size: 3079 2223 988 281 246 HindIII A'AGCT_T Cuts at: 748 2675 748 Size: 1927 4890 HpaI GTT'AAC Cuts at: 4407 4407 Size: 6817 KpnI G_GTAC'C Cuts at: 2836 3730 4142 2836 Size: 894 412 5511 Fragments arranged by size: 5511 894 412 MfeI C'AATT_G Cuts at: 4416 4416 Size: 6817 MluI A'CGCG_T Cuts at: 6693 6693 Size: 6817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4029 5931 6115 1305 Size: 2724 1902 184 2007 Fragments arranged by size: 2724 2007 1902 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2552 2869 3497 10 Size: 54 1425 343 720 317 628 3330 Fragments arranged by size: 3330 1425 720 628 343 317 54 MslI CAynn'nnrTG Cuts at: 518 1166 2897 2981 4775 5134 5293 518 Size: 648 1731 84 1794 359 159 2042 Fragments arranged by size: 2042 1794 1731 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2137 2182 2582 3082 3148 3286 4233 4910 Size: 531 45 400 500 66 138 947 677 Cuts at: 4910 6058 6303 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 531 500 400 245 138 66 45 NcoI C'CATG_G Cuts at: 513 1065 2313 2864 3291 513 Size: 552 1248 551 427 4039 Fragments arranged by size: 4039 1248 552 551 427 NdeI CA'TA_TG Cuts at: 387 387 Size: 6817 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6817 NotI GC'GGCC_GC Cuts at: 4264 4264 Size: 6817 NspI r_CATG'y Cuts at: 1132 1229 2212 2986 3526 3967 4063 4175 5720 Size: 97 983 774 540 441 96 112 1545 Cuts at: 5720 1132 Size: 2229 Fragments arranged by size: 2229 1545 983 774 540 441 112 97 96 PasI CC'CwG_GG Cuts at: 1348 2263 1348 Size: 915 5902 PciI A'CATG_T Cuts at: 3522 4059 5716 3522 Size: 537 1657 4623 Fragments arranged by size: 4623 1657 537 PflMI CCAn_nnn'nTGG Cuts at: 1108 2079 2876 3310 3433 1108 Size: 971 797 434 123 4492 Fragments arranged by size: 4492 971 797 434 123 PfoI T'CCnGG_A Cuts at: 2630 2630 Size: 6817 PmeI GTTT'AAAC Cuts at: 4121 4121 Size: 6817 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 3914 3946 4886 4918 6399 6431 3449 Size: 32 433 32 940 32 1481 32 3835 Fragments arranged by size: 3835 1481 940 433 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3708 3708 Size: 6817 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6817 PsiI TTA'TAA Cuts at: 4387 4387 Size: 6817 PspOMI G'GGCC_C Cuts at: 3338 3338 Size: 6817 PstI C_TGCA'G Cuts at: 830 2021 3253 4169 830 Size: 1191 1232 916 3478 Fragments arranged by size: 3478 1232 1191 916 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6817 PvuII CAG'CTG Cuts at: 3082 3148 3286 3082 Size: 66 138 6613 Fragments arranged by size: 6613 138 66 RsrII CG'GwC_CG Cuts at: 3777 3777 Size: 6817 SacI G_AGCT'C Cuts at: 2616 2976 2616 Size: 360 6457 SalI G'TCGA_C Cuts at: 1936 4159 1936 Size: 2223 4594 SanDI GG'GwC_CC Cuts at: 3708 3708 Size: 6817 SapI GCTCTTCn'nnn_ Cuts at: 2564 2564 Size: 6817 SbfI CC_TGCA'GG Cuts at: 4169 4169 Size: 6817 ScaI AGT'ACT Cuts at: 5065 6777 5065 Size: 1712 5105 SfcI C'TryA_G Cuts at: 826 1045 2017 3112 3249 4165 5300 5981 6172 Size: 219 972 1095 137 916 1135 681 191 Cuts at: 6172 826 Size: 1471 Fragments arranged by size: 1471 1135 1095 972 916 681 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6817 SmaI CCC'GGG Cuts at: 1872 4144 1872 Size: 2272 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 4037 4921 5822 6084 820 Size: 197 926 672 1422 884 901 262 1553 Fragments arranged by size: 1553 1422 926 901 884 672 262 197 SnaBI TAC'GTA Cuts at: 493 4608 493 Size: 4115 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6817 SphI G_CATG'C Cuts at: 1132 2212 2986 4175 1132 Size: 1080 774 1189 3774 Fragments arranged by size: 3774 1189 1080 774 SspI AAT'ATT Cuts at: 5 52 4741 5 Size: 47 4689 2081 Fragments arranged by size: 4689 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6817 StyI C'CwwG_G Cuts at: 513 1065 2313 2836 2864 2875 3291 4256 513 Size: 552 1248 523 28 11 416 965 3074 Fragments arranged by size: 3074 1248 965 552 523 416 28 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3230 3769 4844 4861 5014 5199 5644 Size: 1255 1650 539 1075 17 153 185 445 Cuts at: 5644 325 Size: 1498 Fragments arranged by size: 1650 1498 1255 1075 539 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 3450 5063 Size: 274 80 33 51 1443 1245 228 1613 Cuts at: 5063 6775 96 Size: 1712 138 Fragments arranged by size: 1712 1613 1443 1245 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2106 2138 2933 2965 3083 3115 3158 3190 6777 Size: 32 795 32 118 32 43 32 3587 Cuts at: 6777 6809 2106 Size: 32 2114 Fragments arranged by size: 3587 2114 795 118 43 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6817 XbaI T'CTAG_A Cuts at: 4153 4153 Size: 6817 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2076 2076 Size: 6817 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6817 XmaI C'CCGG_G Cuts at: 1870 4142 1870 Size: 2272 4545 XmnI GAAnn'nnTTC Cuts at: 4047 4946 4047 Size: 899 5918 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3268 4625 276 Size: 53 83 186 936 1734 1357 2468 Fragments arranged by size: 2468 1734 1357 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacII SexAI SfiI SfoI SrfI SwaI