(Circular) (Six-base) MAPSORT of: pFN21AA0595.seq Check: 299 from: 1 to: 9697 pFN21AA0595 9697 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2146 3349 4531 4643 2146 Size: 1203 1182 112 7200 Fragments arranged by size: 7200 1203 1182 112 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7507 278 Size: 53 83 186 936 5971 2468 Fragments arranged by size: 5971 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 3495 3735 4988 7040 1937 Size: 1527 31 240 1253 2052 4594 Fragments arranged by size: 4594 2052 1527 1253 240 31 Acc65I G'GTAC_C Cuts at: 4217 7018 4217 Size: 2801 6896 AclI AA'CG_TT Cuts at: 1563 7824 8197 1563 Size: 6261 373 3063 Fragments arranged by size: 6261 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2752 2978 4197 5286 5487 5813 5820 7760 Size: 1120 226 1219 1089 201 326 7 1940 Cuts at: 7760 9144 9412 1632 Size: 1384 268 1917 Fragments arranged by size: 1940 1917 1384 1219 1120 1089 326 268 226 201 7 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9500 AflIII A'CryG_T Cuts at: 1203 4931 8596 9573 1203 Size: 3728 3665 977 1327 Fragments arranged by size: 3728 3665 1327 977 AgeI A'CCGG_T Cuts at: 5649 8528 5649 Size: 2879 6818 AhdI GACnn_n'nnGTC Cuts at: 8426 8426 Size: 9697 AleI CACnn'nnGTG Cuts at: 1166 6732 1166 Size: 5566 4131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9697 AlwNI CAG_nnn'CTG Cuts at: 2161 2423 2894 3203 5186 6926 7440 9012 2161 Size: 262 471 309 1983 1740 514 1572 2846 Fragments arranged by size: 2846 1983 1740 1572 514 471 309 262 ApaI G_GGCC'C Cuts at: 2060 2216 2568 4947 4961 6020 2060 Size: 156 352 2379 14 1059 5737 Fragments arranged by size: 5737 2379 1059 352 156 14 ApaLI G'TGCA_C Cuts at: 6718 7754 8910 6718 Size: 1036 1156 7505 Fragments arranged by size: 7505 1156 1036 ApoI r'AATT_y Cuts at: 1493 3604 6938 7006 7232 7384 9493 1493 Size: 2111 3334 68 226 152 2109 1697 Fragments arranged by size: 3334 2111 2109 1697 226 152 68 AscI GG'CGCG_CC Cuts at: 2012 2012 Size: 9697 AseI AT'TA_AT Cuts at: 160 1032 8251 160 Size: 872 7219 1606 Fragments arranged by size: 7219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9697 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2478 3567 3579 6054 7022 1870 Size: 73 104 431 1089 12 2475 968 4545 Fragments arranged by size: 4545 2475 1089 968 431 104 73 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3465 3498 3465 Size: 33 9664 BamHI G'GATC_C Cuts at: 7027 7394 7027 Size: 367 9330 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4217 4543 4831 4856 Size: 325 217 627 1529 901 326 288 25 Cuts at: 4856 4894 7018 8473 618 Size: 38 2124 1455 1842 Fragments arranged by size: 2124 1842 1529 1455 901 627 326 325 288 217 38 25 BanII G_rGCy'C Cuts at: 1391 2060 2086 2216 2489 2568 3373 3580 4947 Size: 669 26 130 273 79 805 207 1367 Cuts at: 4947 4961 5146 5688 5882 5960 6020 7016 1391 Size: 14 185 542 194 78 60 996 4072 Fragments arranged by size: 4072 1367 996 805 669 542 273 207 194 185 130 79 78 60 26 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4421 4700 6339 6468 6631 6915 928 Size: 971 2522 279 1639 129 163 284 3710 Fragments arranged by size: 3710 2522 1639 971 284 279 163 129 BbvCI CC'TCA_GC Cuts at: 3440 6100 6190 6777 3440 Size: 2660 90 587 6360 Fragments arranged by size: 6360 2660 587 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6210 6244 7888 7922 1903 Size: 34 4273 34 1644 34 3678 Fragments arranged by size: 4273 3678 1644 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2825 7589 8799 2825 Size: 4764 1210 3723 Fragments arranged by size: 4764 3723 1210 BclI T'GATC_A Cuts at: 1548 3897 6067 1548 Size: 2349 2170 5178 Fragments arranged by size: 5178 2349 2170 BfrBI ATG'CAT Cuts at: 2833 6042 2833 Size: 3209 6488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4771 5678 Size: 107 122 71 631 605 337 2762 907 Cuts at: 5678 6273 8308 136 Size: 595 2035 1525 Fragments arranged by size: 2762 2035 1525 907 631 605 595 337 122 107 71 BglII A'GATC_T Cuts at: 2403 9692 2403 Size: 7289 2408 BlpI GC'TnA_GC Cuts at: 3885 7114 3885 Size: 3229 6468 Bme1580I G_kGCm'C Cuts at: 1790 2060 2216 2568 3213 3321 4614 4836 4897 Size: 270 156 352 645 108 1293 222 61 Cuts at: 4897 4947 4961 5066 6020 6722 7758 8914 1790 Size: 50 14 105 954 702 1036 1156 2573 Fragments arranged by size: 2573 1293 1156 1036 954 702 645 352 270 222 156 108 105 61 50 14 BmgBI CAC'GTC Cuts at: 1318 4088 1318 Size: 2770 6927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 2562 2570 Size: 416 53 83 399 24 1126 19 8 Cuts at: 2570 3083 3899 4046 4341 4668 4904 7428 8386 Size: 513 816 147 295 327 236 2524 958 Cuts at: 8386 442 Size: 1753 Fragments arranged by size: 2524 1753 1126 958 816 513 416 399 327 295 236 147 83 53 24 19 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9697 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2506 2926 5974 2506 Size: 420 3048 6229 Fragments arranged by size: 6229 3048 420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 2698 3062 3101 3206 3533 4026 4998 Size: 1142 317 364 39 105 327 493 972 Cuts at: 4998 5052 5552 5679 8357 1239 Size: 54 500 127 2678 2579 Fragments arranged by size: 2678 2579 1142 972 500 493 364 327 317 127 105 54 39 Bpu10I CC'TnA_GC Cuts at: 3440 4497 6100 6190 6777 3440 Size: 1057 1603 90 587 6360 Fragments arranged by size: 6360 1603 1057 587 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3002 4469 6232 7822 8687 8985 3002 Size: 1467 1763 1590 865 298 3714 Fragments arranged by size: 3714 1763 1590 1467 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2645 2674 3112 4567 5306 5315 8360 Size: 617 1146 29 438 1455 739 9 3045 Cuts at: 8360 882 Size: 2219 Fragments arranged by size: 3045 2219 1455 1146 739 617 438 29 9 BsaAI yAC'GTr Cuts at: 493 1204 5453 7488 493 Size: 711 4249 2035 2702 Fragments arranged by size: 4249 2702 2035 711 BsaBI GATnn'nnATC Cuts at: 6520 9691 6520 Size: 3171 6526 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7504 7886 8521 275 Size: 53 83 186 936 5971 382 635 1451 Fragments arranged by size: 5971 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2065 5649 5904 6309 8130 8528 8802 8949 9360 Size: 3584 255 405 1821 398 274 147 411 Cuts at: 9360 2065 Size: 2402 Fragments arranged by size: 3584 2402 1821 411 405 398 274 255 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4113 4130 4143 4160 6160 Size: 30 165 30 2710 17 13 17 2000 Cuts at: 6160 6190 1178 Size: 30 4685 Fragments arranged by size: 4685 2710 2000 165 30 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2261 2479 2765 2768 2864 2902 3275 Size: 1000 75 218 286 3 96 38 373 Cuts at: 3275 4121 5100 5136 5297 5562 6728 6794 6892 Size: 846 979 36 161 265 1166 66 98 Cuts at: 6892 1186 Size: 3991 Fragments arranged by size: 3991 1166 1000 979 846 373 286 265 218 161 98 96 75 66 38 36 3 BseYI C'CCAG_C Cuts at: 2209 2275 2703 2724 3991 4125 4405 4475 5413 Size: 66 428 21 1267 134 280 70 938 Cuts at: 5413 6019 6919 8900 9396 2209 Size: 606 900 1981 496 2510 Fragments arranged by size: 2510 1981 1267 938 900 606 496 428 280 134 70 66 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 4113 4610 1163 Size: 573 744 1633 497 6250 Fragments arranged by size: 6250 1633 744 573 497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7147 7908 8936 1540 Size: 275 186 5146 761 1028 2301 Fragments arranged by size: 5146 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3132 3373 5146 5387 6722 7758 7843 8914 3132 Size: 241 1773 241 1335 1036 85 1071 3915 Fragments arranged by size: 3915 1773 1335 1071 1036 241 241 85 BsmI GAATG_Cn' Cuts at: 2448 7208 7301 2448 Size: 4760 93 4844 Fragments arranged by size: 4844 4760 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2022 2022 Size: 9697 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2086 2216 2489 2568 3132 3213 Size: 399 270 26 130 273 79 564 81 Cuts at: 3213 3321 3373 3580 4614 4836 4897 4947 4961 Size: 108 52 207 1034 222 61 50 14 Cuts at: 4961 5066 5146 5387 5688 5882 5960 6020 6722 Size: 105 80 241 301 194 78 60 702 Cuts at: 6722 7016 7758 7843 8914 1391 Size: 294 742 85 1071 2174 Fragments arranged by size: 2174 1071 1034 742 702 564 399 301 294 273 270 241 222 207 194 130 108 105 85 81 80 79 78 61 60 52 50 26 14 BspEI T'CCGG_A Cuts at: 2065 5904 9360 2065 Size: 3839 3456 2402 Fragments arranged by size: 3839 3456 2402 BspHI T'CATG_A Cuts at: 7586 9316 7586 Size: 1730 7967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2146 2340 3349 4531 4643 6222 7052 844 Size: 1302 194 1009 1182 112 1579 830 3489 Fragments arranged by size: 3489 1579 1302 1182 1009 830 194 112 BsrBI CCG'CTC Cuts at: 1394 1823 2145 6510 7584 1394 Size: 429 322 4365 1074 3507 Fragments arranged by size: 4365 3507 1074 429 322 BsrDI GCAATG_nn' Cuts at: 65 1248 8192 8366 65 Size: 1183 6944 174 1396 Fragments arranged by size: 6944 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3310 4272 5649 8341 8528 1700 Size: 110 1500 962 1377 2692 187 2869 Fragments arranged by size: 2869 2692 1500 1377 962 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9697 BssHII G'CGCG_C Cuts at: 1925 2012 1925 Size: 87 9610 BssSI C'ACGA_G Cuts at: 4133 7757 8769 4133 Size: 3624 1012 5061 Fragments arranged by size: 5061 3624 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 2503 6598 794 Size: 1367 342 4095 3893 Fragments arranged by size: 4095 3893 1367 342 BstBI TT'CG_AA Cuts at: 8501 8501 Size: 9697 BstEII G'GTnAC_C Cuts at: 1184 3217 1184 Size: 2033 7664 BstXI CCAn_nnnn'nTGG Cuts at: 2836 4369 5530 2836 Size: 1533 1161 7003 Fragments arranged by size: 7003 1533 1161 BstYI r'GATC_y Cuts at: 1972 2403 2614 3084 6094 7027 7394 7779 7796 Size: 431 211 470 3010 933 367 385 17 Cuts at: 7796 9248 9334 9346 9692 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3010 1977 1452 933 470 431 385 367 346 211 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2847 3024 3516 6138 6989 2847 Size: 177 492 2622 851 5555 Fragments arranged by size: 5555 2622 851 492 177 BtgI C'CryG_G Cuts at: 513 1065 2004 2086 5137 5854 513 Size: 552 939 82 3051 717 4356 Fragments arranged by size: 4356 3051 939 717 552 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3087 487 Size: 683 56 1861 7097 Fragments arranged by size: 7097 1861 683 56 BtsI GCAGTG_nn' Cuts at: 2098 2270 2740 2889 3017 3786 3945 4101 4888 Size: 172 470 149 128 769 159 156 787 Cuts at: 4888 5741 6088 7213 8007 8027 2098 Size: 853 347 1125 794 20 3768 Fragments arranged by size: 3768 1125 853 794 787 769 470 347 172 159 156 149 128 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3775 3810 4674 4709 4989 5024 529 Size: 35 3211 35 864 35 280 35 5202 Fragments arranged by size: 5202 3211 864 280 35 35 35 35 DraI TTT'AAA Cuts at: 7001 7001 Size: 9697 DraIII CAC_nnn'GTG Cuts at: 5212 5212 Size: 9697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3645 5900 8704 809 Size: 507 2329 2255 2804 1802 Fragments arranged by size: 2804 2329 2255 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2760 2773 5134 Size: 54 1425 50 275 18 930 13 2361 Cuts at: 5134 5670 5748 6658 6805 7144 8033 9662 8 Size: 536 78 910 147 339 889 1629 43 Fragments arranged by size: 2361 1629 1425 930 910 889 536 339 275 147 78 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 7144 1537 Size: 275 5332 4090 Fragments arranged by size: 5332 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2613 2662 2742 3386 4302 5630 6448 6454 Size: 1260 49 80 644 916 1328 818 6 Cuts at: 6454 6475 7627 1353 Size: 21 1152 3423 Fragments arranged by size: 3423 1328 1260 1152 916 818 644 80 49 21 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6021 8284 8658 8804 387 Size: 1064 138 4432 2263 374 146 1280 Fragments arranged by size: 4432 2263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3371 5144 3371 Size: 1773 7924 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2698 2752 2978 3062 3101 3206 Size: 393 749 317 54 226 84 39 105 Cuts at: 3206 3533 4026 4197 4998 5052 5286 5487 5552 Size: 327 493 171 801 54 234 201 65 Cuts at: 5552 5679 5813 5820 7760 8357 9144 9412 1239 Size: 127 134 7 1940 597 787 268 1524 Fragments arranged by size: 1940 1524 801 787 749 597 493 393 327 317 268 234 226 201 171 134 127 105 84 65 54 54 39 7 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2321 Size: 9697 EcoO109I rG'GnC_Cy Cuts at: 2056 2057 2233 2564 3662 4472 4944 4957 4964 Size: 1 176 331 1098 810 472 13 7 Cuts at: 4964 5640 5913 6372 2056 Size: 676 273 459 5381 Fragments arranged by size: 5381 1098 810 676 472 459 331 273 176 13 7 1 EcoRI G'AATT_C Cuts at: 3604 7006 7384 3604 Size: 3402 378 5917 Fragments arranged by size: 5917 3402 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5362 5452 6459 5362 Size: 90 1007 8600 Fragments arranged by size: 8600 1007 90 FspI TGC'GCA Cuts at: 8203 8203 Size: 9697 HaeII r_GCGC'y Cuts at: 1678 2120 2139 5980 8844 1678 Size: 442 19 3841 2864 2531 Fragments arranged by size: 3841 2864 2531 442 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 3230 3262 4130 Size: 32 135 32 447 32 1179 32 868 Cuts at: 4130 4162 5095 5127 5230 5262 5276 5308 5955 Size: 32 933 32 103 32 14 32 647 Cuts at: 5955 5987 6881 6913 8338 8370 8412 8444 1373 Size: 32 894 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 1179 933 894 868 647 447 135 103 42 32 32 32 32 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2554 3736 3766 3826 3841 3931 Size: 988 281 616 1182 30 60 15 90 Cuts at: 3931 4018 4048 5620 7041 7287 669 Size: 87 30 1572 1421 246 3079 Fragments arranged by size: 3079 1572 1421 1182 988 616 281 246 90 87 60 30 30 15 HindIII A'AGCT_T Cuts at: 748 2666 748 Size: 1918 7779 HpaI GTT'AAC Cuts at: 7287 7287 Size: 9697 KpnI G_GTAC'C Cuts at: 4221 7022 4221 Size: 2801 6896 MfeI C'AATT_G Cuts at: 7296 7296 Size: 9697 MluI A'CGCG_T Cuts at: 9573 9573 Size: 9697 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6330 6924 8811 8995 1305 Size: 5025 594 1887 184 2007 Fragments arranged by size: 5025 2007 1887 594 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2762 2775 5136 5672 5750 Size: 54 1425 343 930 13 2361 536 78 Cuts at: 5750 6807 10 Size: 1057 2900 Fragments arranged by size: 2900 2361 1425 1057 930 536 343 78 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 6732 7655 8014 8173 518 Size: 648 5566 923 359 159 2042 Fragments arranged by size: 5566 2042 923 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2894 3068 3169 3593 5072 5828 6023 Size: 482 806 174 101 424 1479 756 195 Cuts at: 6023 6202 6411 7113 7790 8938 9183 1606 Size: 179 209 702 677 1148 245 2120 Fragments arranged by size: 2120 1479 1148 806 756 702 677 482 424 245 209 195 179 174 101 NcoI C'CATG_G Cuts at: 513 1065 2004 5137 5854 513 Size: 552 939 3133 717 4356 Fragments arranged by size: 4356 3133 939 717 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9697 NotI GC'GGCC_GC Cuts at: 7144 7144 Size: 9697 NsiI A_TGCA'T Cuts at: 2835 6044 2835 Size: 3209 6488 NspI r_CATG'y Cuts at: 1132 1229 4669 4935 4976 5256 7055 8600 1132 Size: 97 3440 266 41 280 1799 1545 2229 Fragments arranged by size: 3440 2229 1799 1545 280 266 97 41 PasI CC'CwG_GG Cuts at: 1348 3294 3830 4108 4961 5331 1348 Size: 1946 536 278 853 370 5714 Fragments arranged by size: 5714 1946 853 536 370 278 PciI A'CATG_T Cuts at: 4931 8596 4931 Size: 3665 6032 PflMI CCAn_nnn'nTGG Cuts at: 1108 2894 1108 Size: 1786 7911 PfoI T'CCnGG_A Cuts at: 2817 3112 3132 2817 Size: 295 20 9382 Fragments arranged by size: 9382 295 20 PmeI GTTT'AAAC Cuts at: 7001 7001 Size: 9697 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2523 2555 7766 7798 9279 9311 2523 Size: 32 5211 32 1481 32 2909 Fragments arranged by size: 5211 2909 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 4472 5913 6372 2233 Size: 2239 1441 459 5558 Fragments arranged by size: 5558 2239 1441 459 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9697 PsiI TTA'TAA Cuts at: 7267 7267 Size: 9697 PspOMI G'GGCC_C Cuts at: 2056 2212 2564 4943 4957 6016 2056 Size: 156 352 2379 14 1059 5737 Fragments arranged by size: 5737 2379 1059 352 156 14 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5446 5478 5446 Size: 32 9665 PstI C_TGCA'G Cuts at: 830 2262 3793 3940 4428 4654 5833 6233 7049 Size: 1432 1531 147 488 226 1179 400 816 Cuts at: 7049 830 Size: 3478 Fragments arranged by size: 3478 1531 1432 1179 816 488 400 226 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9697 PvuII CAG'CTG Cuts at: 2894 3068 3169 3593 5072 5828 6023 6411 2894 Size: 174 101 424 1479 756 195 388 6180 Fragments arranged by size: 6180 1479 756 424 388 195 174 101 RsrII CG'GwC_CG Cuts at: 2063 2063 Size: 9697 SacI G_AGCT'C Cuts at: 3373 5146 3373 Size: 1773 7924 SacII CC_GC'GG Cuts at: 2089 2089 Size: 9697 SalI G'TCGA_C Cuts at: 1936 3734 7039 1936 Size: 1798 3305 4594 Fragments arranged by size: 4594 3305 1798 SapI GCTCTTCn'nnn_ Cuts at: 3386 6448 3386 Size: 3062 6635 SbfI CC_TGCA'GG Cuts at: 4654 6233 7049 4654 Size: 1579 816 7302 Fragments arranged by size: 7302 1579 816 ScaI AGT'ACT Cuts at: 4731 7945 9657 4731 Size: 3214 1712 4771 Fragments arranged by size: 4771 3214 1712 SexAI A'CCwGG_T Cuts at: 3355 3355 Size: 9697 SfcI C'TryA_G Cuts at: 826 1045 2258 3468 3789 3936 4424 4467 4650 Size: 219 1213 1210 321 147 488 43 183 Cuts at: 4650 5598 5829 6229 6412 7045 8180 8861 9052 Size: 948 231 400 183 633 1135 681 191 Cuts at: 9052 826 Size: 1471 Fragments arranged by size: 1471 1213 1210 1135 948 681 633 488 400 321 231 219 191 183 183 147 43 SfiI GGCCn_nnn'nGGCC Cuts at: 6273 6273 Size: 9697 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9697 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9697 SmaI CCC'GGG Cuts at: 1872 2480 3569 6056 7024 1872 Size: 608 1089 2487 968 4545 Fragments arranged by size: 4545 2487 1089 968 608 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 4448 6247 7801 8702 8964 Size: 197 926 1038 1467 1799 1554 901 262 Cuts at: 8964 820 Size: 1553 Fragments arranged by size: 1799 1554 1553 1467 1038 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7488 493 Size: 6995 2702 SpeI A'CTAG_T Cuts at: 152 3152 152 Size: 3000 6697 SphI G_CATG'C Cuts at: 1132 4669 7055 1132 Size: 3537 2386 3774 Fragments arranged by size: 3774 3537 2386 SspI AAT'ATT Cuts at: 5 52 7621 5 Size: 47 7569 2081 Fragments arranged by size: 7569 2081 47 StuI AGG'CCT Cuts at: 1517 6143 1517 Size: 4626 5071 StyI C'CwwG_G Cuts at: 513 1065 2004 2338 3213 3321 3597 4149 4889 Size: 552 939 334 875 108 276 552 740 Cuts at: 4889 4947 5137 5163 5358 5375 5854 6012 6739 Size: 58 190 26 195 17 479 158 727 Cuts at: 6739 7136 513 Size: 397 3074 Fragments arranged by size: 3074 939 875 740 727 552 552 479 397 334 276 195 190 158 108 58 26 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2958 2981 5427 6354 7724 7741 7894 Size: 1255 1378 23 2446 927 1370 17 153 Cuts at: 7894 8079 8524 325 Size: 185 445 1498 Fragments arranged by size: 2446 1498 1378 1370 1255 927 445 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2570 4729 5089 Size: 274 80 33 51 1443 593 2159 360 Cuts at: 5089 7943 9655 96 Size: 2854 1712 138 Fragments arranged by size: 2854 2159 1712 1443 593 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4113 4145 6067 6099 6160 6192 9657 9689 4113 Size: 32 1922 32 61 32 3465 32 4121 Fragments arranged by size: 4121 3465 1922 61 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2033 1316 Size: 717 8980 XbaI T'CTAG_A Cuts at: 3667 7033 3667 Size: 3366 6331 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2120 2619 3301 3862 4335 5879 6393 2120 Size: 499 682 561 473 1544 514 5424 Fragments arranged by size: 5424 1544 682 561 514 499 473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9697 XmaI C'CCGG_G Cuts at: 1870 2478 3567 6054 7022 1870 Size: 608 1089 2487 968 4545 Fragments arranged by size: 4545 2487 1089 968 608 XmnI GAAnn'nnTTC Cuts at: 3525 7826 3525 Size: 4301 5396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7505 276 Size: 53 83 186 936 5971 2468 Fragments arranged by size: 5971 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI SanDI SfoI SrfI SwaI