Restriction map for
pFN21AA0584
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1700 GGTACC G^GTACC
AlwI 1195 GATCC GGATC(4/5)
Asp700I 1641 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1700 GGTACC G^GTACC
AvaI 584 CTCGGG C^[CT]CG[GA]G
AviII 1079 TGCGCA TGC^GCA
BanI 1700 GGTACC G^G[CT][GA]CC
BanII 153 GAGCCC G[GA]GC[CT]^C
BbvCI 50 CCTCAGC CCTCAGC(-5/-2)
BclI 273 TGATCA T^GATCA
BfaI 1285 CTAG C^TAG
BglI 256 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1375 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1375 GCTCAGC GC^T[ACGT]AGC
BsaBI 252 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1717 GAGACC GGTCTC(1/5)
BsaWI 1070 ACCGGA [AT]^CCGG[AT]
BsgI 934 GTGCAG GTGCAG(16/14)
BsoBI 584 CTCGGG C^[CT]CG[GA]G
BspHI 1034 TCATGA T^CATGA
BspMI 872 ACCTGC ACCTGC(4/8)
BsrFI 123 GCCGGT [GA]^CCGG[CT]
BssSI 166 CTCGTG CACGAG(-5/-1)
BstAPI 195 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 455 TTCGAA TT^CGAA
BstXI 397 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1194 AGATCC [GA]^GATC[CT]
CelII 1375 GCTCAGC GC^T[ACGT]AGC
Cfr10I 123 GCCGGT [GA]^CCGG[CT]
DrdI 796 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 726 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 698 CCATGG C^C[AT][AT]GG
FbaI 273 TGATCA T^GATCA
FspI 1079 TGCGCA TGC^GCA
HincII 502 GTTGAC GT[CT]^[GA]AC
HindII 502 GTTGAC GT[CT]^[GA]AC
KpnI 1700 GGTACC GGTAC^C
MaeI 1285 CTAG C^TAG
MaeIII 1709 GTGAC ^GT[ACGT]AC
MamI 252 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1194 AGATCC [GA]^GATC[CT]
MseI 1334 TTAA T^TAA
NcoI 698 CCATGG C^CATGG
NspI 1770 ACATGC [GA]CATG^[CT]
NspV 455 TTCGAA TT^CGAA
RcaI 1034 TCATGA T^CATGA
SexAI 1460 ACCTGGT A^CC[AT]GGT
SfuI 455 TTCGAA TT^CGAA
StyI 698 CCATGG C^C[AT][AT]GG
Tru9I 1334 TTAA T^TAA
Tsp45I 1709 GTGAC ^GT[GC]AC
XhoII 1194 AGATCC [GA]^GATC[CT]
XmnI 1641 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 622 CCTAAGG CC^T[ACGT]AGG
726 CCTCAGG
Bsp1407I 324 TGTACA T^GTACA
1586 TGTACA
BsrBI 94 GAGCGG CCGCTC(-3/-3)
226 GAGCGG
BsrDI 946 GCAATG GCAATG(2/0)
1808 GCAATG
BsrGI 324 TGTACA T^GTACA
1586 TGTACA
BssHII 7 GCGCGC G^CGCGC
79 GCGCGC
Bsu36I 622 CCTAAGG CC^T[ACGT]AGG
726 CCTCAGG
DraIII 277 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
340 CACTATGTG
DsaI 158 CCACGG C^C[GA][CT]GG
698 CCATGG
EarI 672 GAAGAG CTCTTC(1/4)
1410 GAAGAG
Eco81I 622 CCTAAGG CC^T[ACGT]AGG
726 CCTCAGG
HphI 1225 GGTGA GGTGA(8/7)
617 TCACC
Ksp632I 672 GAAGAG CTCTTC(1/4)
1410 GAAGAG
MaeII 653 ACGT A^CGT
1499 ACGT
MslI 834 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
927 CATCCATGTG
MspA1I 1153 CAGCTG C[AC]G^C[GT]G
1378 CAGCTG
PflMI 1724 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1751 CCACCGACTGG
PpuMI 640 AGGACCC [GA]G^G[AT]CC[CT]
1634 GGGACCT
PvuII 1153 CAGCTG CAG^CTG
1378 CAGCTG
SspBI 324 TGTACA T^GTACA
1586 TGTACA
SspI 300 AATATT AAT^ATT
1168 AATATT
Van91I 1724 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1751 CCACCGACTGG
XcmI 158 CCACGGTGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
242 CCAAGAGCAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BalI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MluNI MnlI
MscI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TfiI
TseI Tsp509I TspRI TthHB8I