(Circular) (Six-base) MAPSORT of: pFN21AA0563.seq Check: 6779 from: 1 to: 9607 pFN21AA0563 9607 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7417 278 Size: 53 83 186 936 5881 2468 Fragments arranged by size: 5881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5697 6007 6950 1937 Size: 3760 310 943 4594 Fragments arranged by size: 4594 3760 943 310 Acc65I G'GTAC_C Cuts at: 6928 6928 Size: 9607 AclI AA'CG_TT Cuts at: 1563 7734 8107 1563 Size: 6171 373 3063 Fragments arranged by size: 6171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 2916 4095 4389 5199 6123 7670 9054 Size: 842 442 1179 294 810 924 1547 1384 Cuts at: 9054 9322 1632 Size: 268 1917 Fragments arranged by size: 1917 1547 1384 1179 924 842 810 442 294 268 AflII C'TTAA_G Cuts at: 820 1017 5018 6088 820 Size: 197 4001 1070 4339 Fragments arranged by size: 4339 4001 1070 197 AflIII A'CryG_T Cuts at: 1203 8506 9483 1203 Size: 7303 977 1327 Fragments arranged by size: 7303 1327 977 AgeI A'CCGG_T Cuts at: 8438 8438 Size: 9607 AhdI GACnn_n'nnGTC Cuts at: 2704 3153 8336 2704 Size: 449 5183 3975 Fragments arranged by size: 5183 3975 449 AleI CACnn'nnGTG Cuts at: 1166 2032 1166 Size: 866 8741 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3513 1184 Size: 2329 7278 AlwNI CAG_nnn'CTG Cuts at: 2122 4292 4351 5494 5741 6445 7350 8922 2122 Size: 2170 59 1143 247 704 905 1572 2807 Fragments arranged by size: 2807 2170 1572 1143 905 704 247 59 ApaI G_GGCC'C Cuts at: 2066 2271 2631 3380 5516 6529 2066 Size: 205 360 749 2136 1013 5144 Fragments arranged by size: 5144 2136 1013 749 360 205 ApaLI G'TGCA_C Cuts at: 7664 8820 7664 Size: 1156 8451 ApoI r'AATT_y Cuts at: 1493 2317 4615 6058 6426 6916 7142 7294 9403 Size: 824 2298 1443 368 490 226 152 2109 Cuts at: 9403 1493 Size: 1697 Fragments arranged by size: 2298 2109 1697 1443 824 490 368 226 152 AscI GG'CGCG_CC Cuts at: 5766 5766 Size: 9607 AseI AT'TA_AT Cuts at: 160 1032 3462 6660 8161 160 Size: 872 2430 3198 1501 1606 Fragments arranged by size: 3198 2430 1606 1501 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9607 AvaI C'yCGr_G Cuts at: 1870 1943 3981 6371 6932 1870 Size: 73 2038 2390 561 4545 Fragments arranged by size: 4545 2390 2038 561 73 BamHI G'GATC_C Cuts at: 6937 7304 6937 Size: 367 9240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4598 5579 6928 8383 618 Size: 325 217 627 2811 981 1349 1455 1842 Fragments arranged by size: 2811 1842 1455 1349 981 627 325 217 BanII G_rGCy'C Cuts at: 1391 2066 2181 2271 2631 2670 3380 4503 4584 Size: 675 115 90 360 39 710 1123 81 Cuts at: 4584 5280 5516 6378 6529 6926 1391 Size: 696 236 862 151 397 4072 Fragments arranged by size: 4072 1123 862 710 696 675 397 360 236 151 115 90 81 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2703 3102 3528 4322 5888 6650 Size: 971 279 525 399 426 794 1566 762 Cuts at: 6650 928 Size: 3885 Fragments arranged by size: 3885 1566 971 794 762 525 426 399 279 BbvCI CC'TCA_GC Cuts at: 3522 3522 Size: 9607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7798 7832 1903 Size: 34 5861 34 3678 Fragments arranged by size: 5861 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2586 6622 7499 8709 2586 Size: 4036 877 1210 3484 Fragments arranged by size: 4036 3484 1210 877 BclI T'GATC_A Cuts at: 1548 2257 6415 1548 Size: 709 4158 4740 Fragments arranged by size: 4740 4158 709 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2626 8218 136 Size: 107 122 71 631 605 954 5592 1525 Fragments arranged by size: 5592 1525 954 631 605 122 107 71 BglII A'GATC_T Cuts at: 6813 9602 6813 Size: 2789 6818 BlpI GC'TnA_GC Cuts at: 2115 2469 3504 3798 4128 7024 2115 Size: 354 1035 294 330 2896 4698 Fragments arranged by size: 4698 2896 1035 354 330 294 Bme1580I G_kGCm'C Cuts at: 1790 2024 2066 2271 2631 3380 5516 5584 6359 Size: 234 42 205 360 749 2136 68 775 Cuts at: 6359 6529 7668 8824 1790 Size: 170 1139 1156 2573 Fragments arranged by size: 2573 2136 1156 1139 775 749 360 234 205 170 68 42 BmgBI CAC'GTC Cuts at: 1318 3253 1318 Size: 1935 7672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3222 7338 8296 Size: 416 53 83 399 24 1805 4116 958 Cuts at: 8296 442 Size: 1753 Fragments arranged by size: 4116 1805 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2841 3493 4252 5296 2841 Size: 652 759 1044 7152 Fragments arranged by size: 7152 1044 759 652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2144 2373 2387 2577 2607 2702 2784 Size: 890 15 229 14 190 30 95 82 Cuts at: 2784 2841 3027 3147 3177 3231 3555 3588 3651 Size: 57 186 120 30 54 324 33 63 Cuts at: 3651 3843 4065 4193 4331 4733 5764 6544 8267 Size: 192 222 128 138 402 1031 780 1723 Cuts at: 8267 1239 Size: 2579 Fragments arranged by size: 2579 1723 1031 890 780 402 324 229 222 192 190 186 138 128 120 95 82 63 57 54 33 30 30 15 14 Bpu10I CC'TnA_GC Cuts at: 2175 3522 4404 2175 Size: 1347 882 7378 Fragments arranged by size: 7378 1347 882 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 3609 4131 6090 6725 6844 7732 8597 8895 Size: 835 522 1959 635 119 888 865 298 Cuts at: 8895 2774 Size: 3486 Fragments arranged by size: 3486 1959 888 865 835 635 522 298 119 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 2969 3140 3704 4226 6087 8270 Size: 617 1182 288 171 564 522 1861 2183 Cuts at: 8270 882 Size: 2219 Fragments arranged by size: 2219 2183 1861 1182 617 564 522 288 171 BsaAI yAC'GTr Cuts at: 493 1204 7398 493 Size: 711 6194 2702 Fragments arranged by size: 6194 2702 711 BsaBI GATnn'nnATC Cuts at: 4950 9601 4950 Size: 4651 4956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7414 7796 8431 275 Size: 53 83 186 936 5881 382 635 1451 Fragments arranged by size: 5881 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3451 6498 8040 8438 8712 8859 9270 3451 Size: 3047 1542 398 274 147 411 3788 Fragments arranged by size: 3788 3047 1542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3518 3548 3649 3679 3709 Size: 30 165 30 2115 30 101 30 30 Cuts at: 3709 3739 3841 3871 4006 4036 4057 4060 4087 Size: 30 102 30 135 30 21 3 27 Cuts at: 4087 4090 4309 4339 4460 4490 1178 Size: 3 219 30 121 30 6295 Fragments arranged by size: 6295 2115 219 165 135 121 102 101 30 30 30 30 30 30 30 30 30 30 27 21 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2185 3011 3539 4065 4068 4244 4317 5288 Size: 999 826 528 526 3 176 73 971 Cuts at: 5288 6899 6902 1186 Size: 1611 3 3891 Fragments arranged by size: 3891 1611 999 971 826 528 526 176 73 3 3 BseYI C'CCAG_C Cuts at: 2236 2731 2788 2967 3138 3486 3559 3815 6287 Size: 495 57 179 171 348 73 256 2472 Cuts at: 6287 6438 8810 9306 2236 Size: 151 2372 496 2537 Fragments arranged by size: 2537 2472 2372 496 495 348 256 179 171 151 73 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3275 3303 4439 4440 1163 Size: 573 1539 28 1136 1 6330 Fragments arranged by size: 6330 1539 1136 573 28 1 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7057 7818 8846 1540 Size: 275 186 5056 761 1028 2301 Fragments arranged by size: 5056 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4503 6671 7668 7753 8824 4503 Size: 2168 997 85 1071 5286 Fragments arranged by size: 5286 2168 1071 997 85 BsmI GAATG_Cn' Cuts at: 3610 3967 4132 4896 6296 7118 7211 3610 Size: 357 165 764 1400 822 93 6006 Fragments arranged by size: 6006 1400 822 764 357 165 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6562 6562 Size: 9607 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2066 2181 2271 2631 2670 3380 Size: 399 234 42 115 90 360 39 710 Cuts at: 3380 4503 4584 5280 5516 5584 6359 6378 6529 Size: 1123 81 696 236 68 775 19 151 Cuts at: 6529 6671 6926 7668 7753 8824 1391 Size: 142 255 742 85 1071 2174 Fragments arranged by size: 2174 1123 1071 775 742 710 696 399 360 255 236 234 151 142 115 90 85 81 68 42 39 19 BspEI T'CCGG_A Cuts at: 3451 6498 9270 3451 Size: 3047 2772 3788 Fragments arranged by size: 3788 3047 2772 BspHI T'CATG_A Cuts at: 2836 3172 5031 5214 7496 9226 2836 Size: 336 1859 183 2282 1730 3217 Fragments arranged by size: 3217 2282 1859 1730 336 183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2914 4408 4517 6962 844 Size: 2070 1494 109 2445 3489 Fragments arranged by size: 3489 2445 2070 1494 109 BsrBI CCG'CTC Cuts at: 1394 1823 2015 2143 7494 1394 Size: 429 192 128 5351 3507 Fragments arranged by size: 5351 3507 429 192 128 BsrDI GCAATG_nn' Cuts at: 65 1248 4849 5137 6395 6483 8102 8276 65 Size: 1183 3601 288 1258 88 1619 174 1396 Fragments arranged by size: 3601 1619 1396 1258 1183 288 174 88 BsrFI r'CCGG_y Cuts at: 1700 1810 5484 8251 8438 1700 Size: 110 3674 2767 187 2869 Fragments arranged by size: 3674 2869 2767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9607 BssHII G'CGCG_C Cuts at: 1925 5766 1925 Size: 3841 5766 BssSI C'ACGA_G Cuts at: 7667 8679 7667 Size: 1012 8595 BstAPI GCAn_nnn'nTGC Cuts at: 794 4891 6188 794 Size: 4097 1297 4213 Fragments arranged by size: 4213 4097 1297 BstBI TT'CG_AA Cuts at: 8411 8411 Size: 9607 BstEII G'GTnAC_C Cuts at: 1184 2920 3197 1184 Size: 1736 277 7594 Fragments arranged by size: 7594 1736 277 BstYI r'GATC_y Cuts at: 1972 2461 3646 4945 6813 6937 7304 7689 7706 Size: 489 1185 1299 1868 124 367 385 17 Cuts at: 7706 9158 9244 9256 9602 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1868 1452 1299 1185 489 385 367 346 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3927 4092 6273 3927 Size: 165 2181 7261 Fragments arranged by size: 7261 2181 165 BtgI C'CryG_G Cuts at: 513 1065 2066 2207 2246 4224 513 Size: 552 1001 141 39 1978 5896 Fragments arranged by size: 5896 1978 1001 552 141 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8868 Fragments arranged by size: 8868 683 56 BtsI GCAGTG_nn' Cuts at: 5296 5601 7123 7917 7937 5296 Size: 305 1522 794 20 6966 Fragments arranged by size: 6966 1522 794 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2075 2110 529 Size: 35 1511 35 8026 Fragments arranged by size: 8026 1511 35 35 DraI TTT'AAA Cuts at: 2403 4960 5098 6911 2403 Size: 2557 138 1813 5099 Fragments arranged by size: 5099 2557 1813 138 DraIII CAC_nnn'GTG Cuts at: 3230 3230 Size: 9607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8614 809 Size: 507 7298 1802 Fragments arranged by size: 7298 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 7054 7943 9572 Size: 54 1425 50 275 18 5224 889 1629 Cuts at: 9572 8 Size: 43 Fragments arranged by size: 5224 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7054 1537 Size: 275 5242 4090 Fragments arranged by size: 5242 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2716 3893 6041 7537 1353 Size: 1363 1177 2148 1496 3423 Fragments arranged by size: 3423 2148 1496 1363 1177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4550 8194 8568 8714 387 Size: 1064 138 2961 3644 374 146 1280 Fragments arranged by size: 3644 2961 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4501 4501 Size: 9607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2144 2373 2387 2474 2577 2607 Size: 393 497 15 229 14 87 103 30 Cuts at: 2607 2702 2784 2841 2916 3027 3147 3177 3231 Size: 95 82 57 75 111 120 30 54 Cuts at: 3231 3555 3588 3651 3843 4065 4095 4193 4331 Size: 324 33 63 192 222 30 98 138 Cuts at: 4331 4389 4733 5199 5764 6123 6544 7670 8267 Size: 58 344 466 565 359 421 1126 597 Cuts at: 8267 9054 9322 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1126 787 597 565 497 466 421 393 359 344 324 268 229 222 192 138 120 111 103 98 95 87 82 75 63 58 57 54 33 30 30 30 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 4080 5801 6069 4080 Size: 1721 268 7618 Fragments arranged by size: 7618 1721 268 EcoO109I rG'GnC_Cy Cuts at: 2062 2137 2267 2268 2627 2628 2785 3028 3376 Size: 75 130 1 359 1 157 243 348 Cuts at: 3376 3556 5669 6525 2062 Size: 180 2113 856 5144 Fragments arranged by size: 5144 2113 856 359 348 243 180 157 130 75 1 1 EcoRI G'AATT_C Cuts at: 6916 7294 6916 Size: 378 9229 FspI TGC'GCA Cuts at: 8113 8113 Size: 9607 HaeII r_GCGC'y Cuts at: 1678 8754 1678 Size: 7076 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2168 2200 2690 Size: 32 135 32 427 32 137 32 490 Cuts at: 2690 2722 2939 2971 3110 3139 3142 3171 3518 Size: 32 217 32 139 29 3 29 347 Cuts at: 3518 3550 3703 3735 3837 3869 4057 4089 4225 Size: 32 153 32 102 32 188 32 136 Cuts at: 4225 4251 4257 4283 4309 4339 4341 4371 4511 Size: 26 6 26 26 30 2 30 140 Cuts at: 4511 4543 5393 5425 6000 6032 6805 6837 8248 Size: 32 850 32 575 32 773 32 1411 Cuts at: 8248 8280 8322 8354 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1411 850 773 575 490 427 347 217 188 153 140 139 137 136 135 102 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 29 29 26 26 26 6 3 2 HincII GTy'rAC Cuts at: 669 1657 1938 5395 6951 7197 669 Size: 988 281 3457 1556 246 3079 Fragments arranged by size: 3457 3079 1556 988 281 246 HindIII A'AGCT_T Cuts at: 748 2871 6382 6581 748 Size: 2123 3511 199 3774 Fragments arranged by size: 3774 3511 2123 199 HpaI GTT'AAC Cuts at: 5395 7197 5395 Size: 1802 7805 KpnI G_GTAC'C Cuts at: 6932 6932 Size: 9607 MfeI C'AATT_G Cuts at: 3033 7206 3033 Size: 4173 5434 MluI A'CGCG_T Cuts at: 9483 9483 Size: 9607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2456 2623 3158 3506 3722 3800 3859 4244 Size: 1151 167 535 348 216 78 59 385 Cuts at: 4244 4652 8721 8905 1305 Size: 408 4069 184 2007 Fragments arranged by size: 4069 2007 1151 535 408 385 348 216 184 167 78 59 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7785 Fragments arranged by size: 7785 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2032 5030 5219 6224 7565 7924 8083 Size: 648 866 2998 189 1005 1341 359 159 Cuts at: 8083 518 Size: 2042 Fragments arranged by size: 2998 2042 1341 1005 866 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2050 2149 2351 3503 3521 6287 6445 7023 Size: 444 99 202 1152 18 2766 158 578 Cuts at: 7023 7700 8848 9093 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2766 2120 1152 1148 677 578 444 245 202 158 99 18 NcoI C'CATG_G Cuts at: 513 1065 2066 513 Size: 552 1001 8054 Fragments arranged by size: 8054 1001 552 NdeI CA'TA_TG Cuts at: 387 4493 5073 387 Size: 4106 580 4921 Fragments arranged by size: 4921 4106 580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9607 NotI GC'GGCC_GC Cuts at: 7054 7054 Size: 9607 NspI r_CATG'y Cuts at: 1132 1229 5154 6229 6965 8510 1132 Size: 97 3925 1075 736 1545 2229 Fragments arranged by size: 3925 2229 1545 1075 736 97 PasI CC'CwG_GG Cuts at: 1348 2164 1348 Size: 816 8791 PciI A'CATG_T Cuts at: 8506 8506 Size: 9607 PflMI CCAn_nnn'nTGG Cuts at: 1108 6731 1108 Size: 5623 3984 PfoI T'CCnGG_A Cuts at: 2331 4081 6560 6724 2331 Size: 1750 2479 164 5214 Fragments arranged by size: 5214 2479 1750 164 PmeI GTTT'AAAC Cuts at: 6911 6911 Size: 9607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3975 4007 7676 7708 9189 9221 3975 Size: 32 3669 32 1481 32 4361 Fragments arranged by size: 4361 3669 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2137 Size: 9607 PshAI GACnn'nnGTC Cuts at: 1613 4119 4370 6099 1613 Size: 2506 251 1729 5121 Fragments arranged by size: 5121 2506 1729 251 PsiI TTA'TAA Cuts at: 4829 7177 4829 Size: 2348 7259 PspOMI G'GGCC_C Cuts at: 2062 2267 2627 3376 5512 6525 2062 Size: 205 360 749 2136 1013 5144 Fragments arranged by size: 5144 2136 1013 749 360 205 PspXI vC'TCGA_Gb Cuts at: 3981 3981 Size: 9607 PstI C_TGCA'G Cuts at: 830 2911 3502 6043 6450 6959 830 Size: 2081 591 2541 407 509 3478 Fragments arranged by size: 3478 2541 2081 591 509 407 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9607 PvuII CAG'CTG Cuts at: 2149 2351 3503 6445 2149 Size: 202 1152 2942 5311 Fragments arranged by size: 5311 2942 1152 202 SacI G_AGCT'C Cuts at: 4503 4503 Size: 9607 SalI G'TCGA_C Cuts at: 1936 6949 1936 Size: 5013 4594 SapI GCTCTTCn'nnn_ Cuts at: 6041 6041 Size: 9607 SbfI CC_TGCA'GG Cuts at: 6959 6959 Size: 9607 ScaI AGT'ACT Cuts at: 7855 9567 7855 Size: 1712 7895 SexAI A'CCwGG_T Cuts at: 3781 3951 3781 Size: 170 9437 SfcI C'TryA_G Cuts at: 826 1045 2907 3498 3906 4053 4068 4200 4284 Size: 219 1862 591 408 147 15 132 84 Cuts at: 4284 4302 4320 5190 5472 6039 6446 6955 8090 Size: 18 18 870 282 567 407 509 1135 Cuts at: 8090 8771 8962 826 Size: 681 191 1471 Fragments arranged by size: 1862 1471 1135 870 681 591 567 509 408 407 282 219 191 147 132 84 18 18 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9607 SmaI CCC'GGG Cuts at: 1872 6934 1872 Size: 5062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 3624 3981 4146 5018 6088 Size: 197 926 810 871 357 165 872 1070 Cuts at: 6088 6105 6704 6859 7711 8612 8874 820 Size: 17 599 155 852 901 262 1553 Fragments arranged by size: 1553 1070 926 901 872 871 852 810 599 357 262 197 165 155 17 SnaBI TAC'GTA Cuts at: 493 7398 493 Size: 6905 2702 SpeI A'CTAG_T Cuts at: 152 2101 152 Size: 1949 7658 SphI G_CATG'C Cuts at: 1132 6965 1132 Size: 5833 3774 SspI AAT'ATT Cuts at: 5 52 2555 7531 5 Size: 47 2503 4976 2081 Fragments arranged by size: 4976 2503 2081 47 StuI AGG'CCT Cuts at: 1517 4475 4737 4880 1517 Size: 2958 262 143 6244 Fragments arranged by size: 6244 2958 262 143 StyI C'CwwG_G Cuts at: 513 1065 2066 4621 5516 5636 5760 5961 7046 Size: 552 1001 2555 895 120 124 201 1085 Cuts at: 7046 513 Size: 3074 Fragments arranged by size: 3074 2555 1085 1001 895 552 201 124 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3241 3829 4451 4604 4691 5732 5974 Size: 1255 1661 588 622 153 87 1041 242 Cuts at: 5974 7634 7651 7804 7989 8434 325 Size: 1660 17 153 185 445 1498 Fragments arranged by size: 1661 1660 1498 1255 1041 622 588 445 242 185 153 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2561 5357 7853 Size: 274 80 33 51 1443 584 2796 2496 Cuts at: 7853 9565 96 Size: 1712 138 Fragments arranged by size: 2796 2496 1712 1443 584 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2498 2530 4460 4492 5158 5190 5825 5857 9567 Size: 32 1930 32 666 32 635 32 3710 Cuts at: 9567 9599 2498 Size: 32 2506 Fragments arranged by size: 3710 2506 1930 666 635 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9607 XbaI T'CTAG_A Cuts at: 2662 4981 6943 2662 Size: 2319 1962 5326 Fragments arranged by size: 5326 2319 1962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2244 2472 2244 Size: 228 9379 XhoI C'TCGA_G Cuts at: 1943 3981 1943 Size: 2038 7569 XmaI C'CCGG_G Cuts at: 1870 6932 1870 Size: 5062 4545 XmnI GAAnn'nnTTC Cuts at: 5753 7736 5753 Size: 1983 7624 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7415 276 Size: 53 83 186 936 5881 2468 Fragments arranged by size: 5881 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI