Restriction map for
pFN21AA0562
(Circular) (Six-base) MAPSORT of: pFN21AA0562.seq Check: 6007 from: 1 to: 7480
pFN21AA0562 7480 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5290 278
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3081 4823 1937
Size: 1144 1742 4594
Fragments arranged by size:
4594 1742 1144
Acc65I G'GTAC_C
Cuts at: 4801 4801
Size: 7480
AclI AA'CG_TT
Cuts at: 1563 5607 5980 1563
Size: 4044 373 3063
Fragments arranged by size:
4044 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2044 3011 3726 4167 4242 4543 5543 6927
Size: 412 967 715 441 75 301 1000 1384
Cuts at: 6927 7195 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1000 967 715 441 412 301
268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7283
AflIII A'CryG_T
Cuts at: 1203 2295 3909 6379 7356 1203
Size: 1092 1614 2470 977 1327
Fragments arranged by size:
2470 1614 1327 1092 977
AgeI A'CCGG_T
Cuts at: 4533 6311 4533
Size: 1778 5702
AhdI GACnn_n'nnGTC
Cuts at: 6209 6209
Size: 7480
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 2859 1184
Size: 1021 654 5805
Fragments arranged by size:
5805 1021 654
AlwNI CAG_nnn'CTG
Cuts at: 4037 4217 5223 6795 4037
Size: 180 1006 1572 4722
Fragments arranged by size:
4722 1572 1006 180
ApaI G_GGCC'C
Cuts at: 4601 4601
Size: 7480
ApaLI G'TGCA_C
Cuts at: 5537 6693 5537
Size: 1156 6324
ApoI r'AATT_y
Cuts at: 1493 3652 4137 4789 5015 5167 7276 1493
Size: 2159 485 652 226 152 2109 1697
Fragments arranged by size:
2159 2109 1697 652 485 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6034 160
Size: 872 5002 1606
Fragments arranged by size:
5002 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2334 2499 3811 4805 1870
Size: 73 124 267 165 1312 994 4545
Fragments arranged by size:
4545 1312 994 267 165 124 73
BamHI G'GATC_C
Cuts at: 4810 5177 4810
Size: 367 7113
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4801 6256 618
Size: 325 217 627 3014 1455 1842
Fragments arranged by size:
3014 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2076 3261 4601 4757 4799 1391
Size: 685 1185 1340 156 42 4072
Fragments arranged by size:
4072 1340 1185 685 156 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 2290 2802 2908 928
Size: 971 159 232 512 106 5500
Fragments arranged by size:
5500 971 512 232 159 106
BbvCI CC'TCA_GC
Cuts at: 3066 3327 3066
Size: 261 7219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2528 2562 4603 4637 5671
Size: 34 50 34 507 34 2041 34 1034
Cuts at: 5671 5705 1903
Size: 34 3678
Fragments arranged by size:
3678 2041 1034 507 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5372 6582 5372
Size: 1210 6270
BclI T'GATC_A
Cuts at: 1548 3522 1548
Size: 1974 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4605 6091 136
Size: 107 122 71 631 605 2933 1486 1525
Fragments arranged by size:
2933 1525 1486 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7475 7475
Size: 7480
BlpI GC'TnA_GC
Cuts at: 4675 4897 4675
Size: 222 7258
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2532 3803 3856 4051 4601 5541 6697
Size: 278 464 1271 53 195 550 940 1156
Cuts at: 6697 1790
Size: 2573
Fragments arranged by size:
2573 1271 1156 940 550 464 278 195
53
BmgBI CAC'GTC
Cuts at: 1318 3345 1318
Size: 2027 5453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5211 6169 442
Size: 416 53 83 399 24 3794 958 1753
Fragments arranged by size:
3794 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2885 2906 3636 3878 3936 3959 4587 4741
Size: 1646 21 730 242 58 23 628 154
Cuts at: 4741 6140 1239
Size: 1399 2579
Fragments arranged by size:
2579 1646 1399 730 628 242 154 58
23 21
Bpu10I CC'TnA_GC
Cuts at: 3066 3327 3066
Size: 261 7219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 5605 6470 6768 3298
Size: 2307 865 298 4010
Fragments arranged by size:
4010 2307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 6143 882
Size: 617 955 3689 2219
Fragments arranged by size:
3689 2219 955 617
BsaAI yAC'GTr
Cuts at: 493 1204 2296 5271 493
Size: 711 1092 2975 2702
Fragments arranged by size:
2975 2702 1092 711
BsaBI GATnn'nnATC
Cuts at: 3108 7474 3108
Size: 4366 3114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5287 5669 6304 275
Size: 53 83 186 936 3754 382 635 1451
Fragments arranged by size:
3754 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4516 4533 5913 6311 6585 6732 7143 4516
Size: 17 1380 398 274 147 411 4853
Fragments arranged by size:
4853 1380 411 398 274 147 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3046 3076 1178
Size: 30 165 30 1643 30 5582
Fragments arranged by size:
5582 1643 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3054 1186
Size: 1868 5612
BseYI C'CCAG_C
Cuts at: 4600 6683 7179 4600
Size: 2083 496 4901
Fragments arranged by size:
4901 2083 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2855 3740 4594 1163
Size: 573 1119 885 854 4049
Fragments arranged by size:
4049 1119 885 854 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4930 5691 6719 1540
Size: 275 186 2929 761 1028 2301
Fragments arranged by size:
2929 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 4248 4398 4757 5541 5626 6697 2076
Size: 2172 150 359 784 85 1071 2859
Fragments arranged by size:
2859 2172 1071 784 359 150 85
BsiWI C'GTAC_G
Cuts at: 2562 4767 2562
Size: 2205 5275
BsmI GAATG_Cn'
Cuts at: 3070 4991 5084 3070
Size: 1921 93 5466
Fragments arranged by size:
5466 1921 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3878 3878
Size: 7480
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2076 2532 3261 3803 3856 4051
Size: 399 278 8 456 729 542 53 195
Cuts at: 4051 4248 4398 4601 4757 4799 5541 5626 6697
Size: 197 150 203 156 42 742 85 1071
Cuts at: 6697 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 729 542 456 399 278
203 197 195 156 150 85 53 42
8
BspEI T'CCGG_A
Cuts at: 7143 7143
Size: 7480
BspHI T'CATG_A
Cuts at: 2076 4549 5369 7099 2076
Size: 2473 820 1730 2457
Fragments arranged by size:
2473 2457 1730 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4629 4835 844
Size: 3785 206 3489
Fragments arranged by size:
3785 3489 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 3148 5367 1394
Size: 429 452 873 2219 3507
Fragments arranged by size:
3507 2219 873 452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 3762 5975 6149 65
Size: 1183 1473 1041 2213 174 1396
Fragments arranged by size:
2213 1473 1396 1183 1041 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4533 4604 6124 6311 1700
Size: 110 2723 71 1520 187 2869
Fragments arranged by size:
2869 2723 1520 187 110 71
BsrGI T'GTAC_A
Cuts at: 96 3912 96
Size: 3816 3664
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7480
BssSI C'ACGA_G
Cuts at: 5540 6552 5540
Size: 1012 6468
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2949 3371 4217 794
Size: 2155 422 846 4057
Fragments arranged by size:
4057 2155 846 422
BstBI TT'CG_AA
Cuts at: 2281 3895 6284 2281
Size: 1614 2389 3477
Fragments arranged by size:
3477 2389 1614
BstEII G'GTnAC_C
Cuts at: 1184 2109 1184
Size: 925 6555
BstXI CCAn_nnnn'nTGG
Cuts at: 2018 2525 2018
Size: 507 6973
BstYI r'GATC_y
Cuts at: 1972 2623 3103 4312 4722 4810 5177 5562 5579
Size: 651 480 1209 410 88 367 385 17
Cuts at: 5579 7031 7117 7129 7475 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1209 651 480 410 385 367
346 88 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2784 513
Size: 552 1719 5209
Fragments arranged by size:
5209 1719 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3237 487
Size: 683 56 2011 4730
Fragments arranged by size:
4730 2011 683 56
BtsI GCAGTG_nn'
Cuts at: 3192 3365 4357 4456 4655 4996 5790 5810 3192
Size: 173 992 99 199 341 794 20 4862
Fragments arranged by size:
4862 992 794 341 199 173 99 20
ClaI AT'CG_AT
Cuts at: 1554 3300 1554
Size: 1746 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7445
DraI TTT'AAA
Cuts at: 3426 3679 4784 3426
Size: 253 1105 6122
Fragments arranged by size:
6122 1105 253
DraIII CAC_nnn'GTG
Cuts at: 3580 3580
Size: 7480
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6487 809
Size: 507 5171 1802
Fragments arranged by size:
5171 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4683 4927 5816
Size: 54 1425 50 275 18 2853 244 889
Cuts at: 5816 7445 8
Size: 1629 43
Fragments arranged by size:
2853 1629 1425 889 275 244 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4927 1537
Size: 275 3115 4090
Fragments arranged by size:
4090 3115 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3159 3457 3745 4464 4566 5410 1353
Size: 1806 298 288 719 102 844 3423
Fragments arranged by size:
3423 1806 844 719 298 288 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4755 6067 6441 6587 387
Size: 1064 138 3166 1312 374 146 1280
Fragments arranged by size:
3166 1312 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2074 4755 2074
Size: 2681 4799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2885 2906 3011 3636 3726 3878
Size: 393 412 841 21 105 625 90 152
Cuts at: 3878 3936 3959 4167 4242 4543 4587 4741 5543
Size: 58 23 208 75 301 44 154 802
Cuts at: 5543 6140 6927 7195 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 841 802 787 625 597 412 393
301 268 208 154 152 105 90 75
58 44 23 21
EcoO109I rG'GnC_Cy
Cuts at: 2542 4648 4694 2542
Size: 2106 46 5328
Fragments arranged by size:
5328 2106 46
EcoRI G'AATT_C
Cuts at: 4789 5167 4789
Size: 378 7102
EcoRV GAT'ATC
Cuts at: 4380 4380
Size: 7480
FspI TGC'GCA
Cuts at: 4625 5986 4625
Size: 1361 6119
HaeII r_GCGC'y
Cuts at: 1678 2752 6627 1678
Size: 1074 3875 2531
Fragments arranged by size:
3875 2531 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2570 2602 3432
Size: 32 135 32 441 32 525 32 830
Cuts at: 3432 3464 4369 4401 6121 6153 6195 6227 1373
Size: 32 905 32 1720 32 42 32 2626
Fragments arranged by size:
2626 1720 905 830 525 441 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2407 4824 5070 669
Size: 988 281 469 2417 246 3079
Fragments arranged by size:
3079 2417 988 469 281 246
HindIII A'AGCT_T
Cuts at: 748 2239 4104 748
Size: 1491 1865 4124
Fragments arranged by size:
4124 1865 1491
HpaI GTT'AAC
Cuts at: 5070 5070
Size: 7480
KpnI G_GTAC'C
Cuts at: 4805 4805
Size: 7480
MfeI C'AATT_G
Cuts at: 5079 5079
Size: 7480
MluI A'CGCG_T
Cuts at: 7356 7356
Size: 7480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3385 6594 6778 1305
Size: 2080 3209 184 2007
Fragments arranged by size:
3209 2080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5658
Fragments arranged by size:
5658 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 5438 5797 5956 518
Size: 648 2136 2136 359 159 2042
Fragments arranged by size:
2136 2136 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2863 3647 4120 4679 4896 5573 6721 6966
Size: 1257 784 473 559 217 677 1148 245
Cuts at: 6966 1606
Size: 2120
Fragments arranged by size:
2120 1257 1148 784 677 559 473 245
217
NaeI GCC'GGC
Cuts at: 4606 4606
Size: 7480
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6928
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7480
NgoMIV G'CCGG_C
Cuts at: 4604 4604
Size: 7480
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7480
NotI GC'GGCC_GC
Cuts at: 4927 4927
Size: 7480
NspI r_CATG'y
Cuts at: 1132 1229 3244 3913 4838 6383 1132
Size: 97 2015 669 925 1545 2229
Fragments arranged by size:
2229 2015 1545 925 669 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7480
PciI A'CATG_T
Cuts at: 3909 6379 3909
Size: 2470 5010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7480
PfoI T'CCnGG_A
Cuts at: 2880 3936 4236 2880
Size: 1056 300 6124
Fragments arranged by size:
6124 1056 300
PmeI GTTT'AAAC
Cuts at: 4784 4784
Size: 7480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5549 5581 7062 7094 5549
Size: 32 1481 32 5935
Fragments arranged by size:
5935 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 2542
Size: 7480
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7480
PsiI TTA'TAA
Cuts at: 5050 5050
Size: 7480
PspOMI G'GGCC_C
Cuts at: 4597 4597
Size: 7480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 7448
PstI C_TGCA'G
Cuts at: 830 2184 2470 3079 4644 4662 4832 830
Size: 1354 286 609 1565 18 170 3478
Fragments arranged by size:
3478 1565 1354 609 286 170 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7480
PvuII CAG'CTG
Cuts at: 2863 3647 4120 4679 2863
Size: 784 473 559 5664
Fragments arranged by size:
5664 784 559 473
SacI G_AGCT'C
Cuts at: 2076 4757 2076
Size: 2681 4799
SalI G'TCGA_C
Cuts at: 1936 4822 1936
Size: 2886 4594
SapI GCTCTTCn'nnn_
Cuts at: 3457 4464 3457
Size: 1007 6473
SbfI CC_TGCA'GG
Cuts at: 4832 4832
Size: 7480
ScaI AGT'ACT
Cuts at: 5728 7440 5728
Size: 1712 5768
SexAI A'CCwGG_T
Cuts at: 3714 3714
Size: 7480
SfcI C'TryA_G
Cuts at: 826 1045 2180 2466 3075 4029 4065 4640 4658
Size: 219 1135 286 609 954 36 575 18
Cuts at: 4658 4828 5963 6644 6835 826
Size: 170 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 954 681 609 575 286
219 191 170 36 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7480
SmaI CCC'GGG
Cuts at: 1872 2069 2336 4807 1872
Size: 197 267 2471 4545
Fragments arranged by size:
4545 2471 267 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 3277 5584 6485 6747 820
Size: 197 926 556 778 2307 901 262 1553
Fragments arranged by size:
2307 1553 926 901 778 556 262 197
SnaBI TAC'GTA
Cuts at: 493 5271 493
Size: 4778 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7480
SphI G_CATG'C
Cuts at: 1132 4838 1132
Size: 3706 3774
SspI AAT'ATT
Cuts at: 5 52 2252 5404 5
Size: 47 2200 3152 2081
Fragments arranged by size:
3152 2200 2081 47
StuI AGG'CCT
Cuts at: 1517 3275 3332 1517
Size: 1758 57 5665
Fragments arranged by size:
5665 1758 57
StyI C'CwwG_G
Cuts at: 513 1065 2524 2752 2811 3318 4698 4919 513
Size: 552 1459 228 59 507 1380 221 3074
Fragments arranged by size:
3074 1459 1380 552 507 228 221 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 5507 5524 5677 5862 6307 325
Size: 1255 511 3416 17 153 185 445 1498
Fragments arranged by size:
3416 1498 1255 511 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3912 5726 7438
Size: 274 80 33 51 1443 1935 1814 1712
Cuts at: 7438 96
Size: 138
Fragments arranged by size:
1935 1814 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3914 3946 7440 7472 3914
Size: 32 3494 32 3922
Fragments arranged by size:
3922 3494 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3346 1316
Size: 2030 5450
XbaI T'CTAG_A
Cuts at: 2551 4252 4816 2551
Size: 1701 564 5215
Fragments arranged by size:
5215 1701 564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3304 3304
Size: 7480
XhoI C'TCGA_G
Cuts at: 1943 2499 1943
Size: 556 6924
XmaI C'CCGG_G
Cuts at: 1870 2067 2334 4805 1870
Size: 197 267 2471 4545
Fragments arranged by size:
4545 2471 267 197
XmnI GAAnn'nnTTC
Cuts at: 5609 5609
Size: 7480
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5288 276
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI RsrII SacII
SanDI SfiI SfoI SrfI SwaI