Restriction map for
pFN21AA0541
(Linear) (Six-base) MAPSORT of: KIAA0541.seq Check: 7357 from: 1 to: 4470
KIAA0541.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 2132 3269 4470
Size: 577 1555 1137 1201
Fragments arranged by size:
1555 1201 1137 577
AflIII A'CryG_T
Cuts at: 0 1402 2064 4274 4470
Size: 1402 662 2210 196
Fragments arranged by size:
2210 1402 662 196
AleI CACnn'nnGTG
Cuts at: 0 4195 4470
Size: 4195 275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4315 4470
Size: 4315 155
AlwNI CAG_nnn'CTG
Cuts at: 0 2614 3087 3247 3745 4470
Size: 2614 473 160 498 725
Fragments arranged by size:
2614 725 498 473 160
ApoI r'AATT_y
Cuts at: 0 307 915 1992 2154 3007 3335 3406 3464
Size: 307 608 1077 162 853 328 71 58
Cuts at: 3464 3914 3932 4470
Size: 450 18 538
Fragments arranged by size:
1077 853 608 538 450 328 307 162
71 58 18
AseI AT'TA_AT
Cuts at: 0 161 4470
Size: 161 4309
AvaI C'yCGr_G
Cuts at: 0 167 4470
Size: 167 4303
BamHI G'GATC_C
Cuts at: 0 3496 4470
Size: 3496 974
BanI G'GyrC_C
Cuts at: 0 4311 4344 4470
Size: 4311 33 126
Fragments arranged by size:
4311 126 33
BanII G_rGCy'C
Cuts at: 0 2194 3153 3739 4116 4470
Size: 2194 959 586 377 354
Fragments arranged by size:
2194 959 586 377 354
BbsI GAAGACnn'nnnn_
Cuts at: 0 4027 4470
Size: 4027 443
BbvCI CC'TCA_GC
Cuts at: 0 4316 4470
Size: 4316 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3667 3701 3731 3765 4470
Size: 3667 34 30 34 705
Fragments arranged by size:
3667 705 34 34 30
BciVI GTATCCnnnnn_n'
Cuts at: 0 591 1761 4470
Size: 591 1170 2709
Fragments arranged by size:
2709 1170 591
BclI T'GATC_A
Cuts at: 0 1440 2733 2883 4470
Size: 1440 1293 150 1587
Fragments arranged by size:
1587 1440 1293 150
BfrBI ATG'CAT
Cuts at: 0 1655 4470
Size: 1655 2815
BglI GCCn_nnn'nGGC
Cuts at: 0 3082 4470
Size: 3082 1388
Bme1580I G_kGCm'C
Cuts at: 0 4349 4470
Size: 4349 121
BmgBI CAC'GTC
Cuts at: 0 3180 3619 4277 4470
Size: 3180 439 658 193
Fragments arranged by size:
3180 658 439 193
BmrI ACTGGGnnnn_n'
Cuts at: 0 3004 4470
Size: 3004 1466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1481 1523 3046 3212 4470
Size: 1481 42 1523 166 1258
Fragments arranged by size:
1523 1481 1258 166 42
Bpu10I CC'TnA_GC
Cuts at: 0 4316 4470
Size: 4316 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4372 4470
Size: 4372 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 1431 4470
Size: 1431 3039
BsaBI GATnn'nnATC
Cuts at: 0 2882 4470
Size: 2882 1588
BsaWI w'CCGG_w
Cuts at: 0 4145 4470
Size: 4145 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2654 2684 3213 3243 4470
Size: 2654 30 529 30 1227
Fragments arranged by size:
2654 1227 529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 2302 2311 2534 4470
Size: 631 1671 9 223 1936
Fragments arranged by size:
1936 1671 631 223 9
BseYI C'CCAG_C
Cuts at: 0 3738 4050 4284 4470
Size: 3738 312 234 186
Fragments arranged by size:
3738 312 234 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2475 2837 4375 4470
Size: 2475 362 1538 95
Fragments arranged by size:
2475 1538 362 95
BsiEI CG_ry'CG
Cuts at: 0 101 4470
Size: 101 4369
BsiHKAI G_wGCw'C
Cuts at: 0 174 4116 4457 4470
Size: 174 3942 341 13
Fragments arranged by size:
3942 341 174 13
BsmI GAATG_Cn'
Cuts at: 0 2480 4470
Size: 2480 1990
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1893 4470
Size: 1893 2577
Bsp1286I G_dGCh'C
Cuts at: 0 174 2194 3153 3739 4116 4349 4457 4470
Size: 174 2020 959 586 377 233 108 13
Fragments arranged by size:
2020 959 586 377 233 174 108 13
BspEI T'CCGG_A
Cuts at: 0 4145 4470
Size: 4145 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 864 1308 4052 4470
Size: 864 444 2744 418
Fragments arranged by size:
2744 864 444 418
BsrBI CCG'CTC
Cuts at: 0 2869 3758 4089 4470
Size: 2869 889 331 381
Fragments arranged by size:
2869 889 381 331
BsrDI GCAATG_nn'
Cuts at: 0 57 1121 3782 4470
Size: 57 1064 2661 688
Fragments arranged by size:
2661 1064 688 57
BsrGI T'GTAC_A
Cuts at: 0 930 1238 4470
Size: 930 308 3232
Fragments arranged by size:
3232 930 308
BssSI C'ACGA_G
Cuts at: 0 3899 3973 4470
Size: 3899 74 497
Fragments arranged by size:
3899 497 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3879 4470
Size: 3879 591
BstYI r'GATC_y
Cuts at: 0 138 3496 3966 4012 4470
Size: 138 3358 470 46 458
Fragments arranged by size:
3358 470 458 138 46
Bsu36I CC'TnA_GG
Cuts at: 0 3240 3470 4470
Size: 3240 230 1000
Fragments arranged by size:
3240 1000 230
BtgI C'CryG_G
Cuts at: 0 393 1380 3224 4119 4170 4470
Size: 393 987 1844 895 51 300
Fragments arranged by size:
1844 987 895 393 300 51
BtsI GCAGTG_nn'
Cuts at: 0 897 1590 2393 2487 4190 4202 4279 4470
Size: 897 693 803 94 1703 12 77 191
Fragments arranged by size:
1703 897 803 693 191 94 77 12
ClaI AT'CG_AT
Cuts at: 0 3054 4470
Size: 3054 1416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3942 3977 4470
Size: 3942 35 493
Fragments arranged by size:
3942 493 35
DraIII CAC_nnn'GTG
Cuts at: 0 1381 4470
Size: 1381 3089
DrdI GACnn_nn'nnGTC
Cuts at: 0 128 4470
Size: 128 4342
EaeI y'GGCC_r
Cuts at: 0 1313 1360 3512 4470
Size: 1313 47 2152 958
Fragments arranged by size:
2152 1313 958 47
EarI CTCTTCn'nnn_
Cuts at: 0 1347 2230 2279 3263 4470
Size: 1347 883 49 984 1207
Fragments arranged by size:
1347 1207 984 883 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3112 4470
Size: 3112 1358
EcoICRI GAG'CTC
Cuts at: 0 4114 4470
Size: 4114 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1481 1523 2132 3046 3212 3269 4470
Size: 577 904 42 609 914 166 57 1201
Fragments arranged by size:
1201 914 904 609 577 166 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2788 4470
Size: 2788 1682
EcoO109I rG'GnC_Cy
Cuts at: 0 754 2749 3084 3244 4470
Size: 754 1995 335 160 1226
Fragments arranged by size:
1995 1226 754 335 160
EcoRV GAT'ATC
Cuts at: 0 467 1457 4222 4470
Size: 467 990 2765 248
Fragments arranged by size:
2765 990 467 248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 400 677 4470
Size: 400 277 3793
Fragments arranged by size:
3793 400 277
HaeII r_GCGC'y
Cuts at: 0 52 4470
Size: 52 4418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 781 813 1890 1922 3432 3464
Size: 607 32 142 32 1077 32 1510 32
Cuts at: 3464 4470
Size: 1006
Fragments arranged by size:
1510 1077 1006 607 142 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1878 3285 3485 4470
Size: 1878 1407 200 985
Fragments arranged by size:
1878 1407 985 200
HindIII A'AGCT_T
Cuts at: 0 215 4470
Size: 215 4255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2253 2833 2929 3524 4089 4439 4470
Size: 2253 580 96 595 565 350 31
Fragments arranged by size:
2253 595 580 565 350 96 31
MscI TGG'CCA
Cuts at: 0 1362 3514 4470
Size: 1362 2152 956
Fragments arranged by size:
2152 1362 956
MslI CAynn'nnrTG
Cuts at: 0 1535 2065 3649 3718 3994 4195 4297 4432
Size: 1535 530 1584 69 276 201 102 135
Cuts at: 4432 4470
Size: 38
Fragments arranged by size:
1584 1535 530 276 201 135 102 69
38
MspA1I CmG'CkG
Cuts at: 0 70 1084 1188 1326 3658 3745 4320 4470
Size: 70 1014 104 138 2332 87 575 150
Fragments arranged by size:
2332 1014 575 150 138 104 87 70
NcoI C'CATG_G
Cuts at: 0 4119 4170 4470
Size: 4119 51 300
Fragments arranged by size:
4119 300 51
NdeI CA'TA_TG
Cuts at: 0 2528 2738 4470
Size: 2528 210 1732
Fragments arranged by size:
2528 1732 210
NsiI A_TGCA'T
Cuts at: 0 1657 4470
Size: 1657 2813
NspI r_CATG'y
Cuts at: 0 594 1347 1406 2068 3772 4302 4470
Size: 594 753 59 662 1704 530 168
Fragments arranged by size:
1704 753 662 594 530 168 59
PciI A'CATG_T
Cuts at: 0 1402 2064 4470
Size: 1402 662 2406
Fragments arranged by size:
2406 1402 662
PflMI CCAn_nnn'nTGG
Cuts at: 0 710 1252 4470
Size: 710 542 3218
Fragments arranged by size:
3218 710 542
PfoI T'CCnGG_A
Cuts at: 0 3260 4470
Size: 3260 1210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1331 1363 2204 2236 4470
Size: 1331 32 841 32 2234
Fragments arranged by size:
2234 1331 841 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 754 2749 4470
Size: 754 1995 1721
Fragments arranged by size:
1995 1721 754
PsiI TTA'TAA
Cuts at: 0 2552 4470
Size: 2552 1918
PspXI vC'TCGA_Gb
Cuts at: 0 167 4470
Size: 167 4303
PstI C_TGCA'G
Cuts at: 0 4063 4197 4470
Size: 4063 134 273
Fragments arranged by size:
4063 273 134
PvuI CG_AT'CG
Cuts at: 0 101 4470
Size: 101 4369
PvuII CAG'CTG
Cuts at: 0 1084 1188 1326 3745 4320 4470
Size: 1084 104 138 2419 575 150
Fragments arranged by size:
2419 1084 575 150 138 104
SacI G_AGCT'C
Cuts at: 0 4116 4470
Size: 4116 354
SapI GCTCTTCn'nnn_
Cuts at: 0 3263 4470
Size: 3263 1207
ScaI AGT'ACT
Cuts at: 0 2444 4470
Size: 2444 2026
SexAI A'CCwGG_T
Cuts at: 0 1721 4340 4470
Size: 1721 2619 130
Fragments arranged by size:
2619 1721 130
SfcI C'TryA_G
Cuts at: 0 1572 1604 3550 4059 4193 4470
Size: 1572 32 1946 509 134 277
Fragments arranged by size:
1946 1572 509 277 134 32
SmlI C'TyrA_G
Cuts at: 0 167 4387 4470
Size: 167 4220 83
Fragments arranged by size:
4220 167 83
SpeI A'CTAG_T
Cuts at: 0 3487 4470
Size: 3487 983
SphI G_CATG'C
Cuts at: 0 3772 4302 4470
Size: 3772 530 168
Fragments arranged by size:
3772 530 168
SspI AAT'ATT
Cuts at: 0 611 709 1015 1062 2005 2823 4470
Size: 611 98 306 47 943 818 1647
Fragments arranged by size:
1647 943 818 611 306 98 47
StuI AGG'CCT
Cuts at: 0 1707 3015 4470
Size: 1707 1308 1455
Fragments arranged by size:
1707 1455 1308
StyI C'CwwG_G
Cuts at: 0 2469 4119 4170 4470
Size: 2469 1650 51 300
Fragments arranged by size:
2469 1650 300 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2330 2861 4470
Size: 2330 531 1609
Fragments arranged by size:
2330 1609 531
TatI w'GTAC_w
Cuts at: 0 930 1238 1320 1584 2442 3167 3998 4470
Size: 930 308 82 264 858 725 831 472
Fragments arranged by size:
930 858 831 725 472 308 264 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2654 2686 3213 3245 4470
Size: 2654 32 527 32 1225
Fragments arranged by size:
2654 1225 527 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 793 1904 3178 4470
Size: 793 1111 1274 1292
Fragments arranged by size:
1292 1274 1111 793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 358 3507 3717 3960 4470
Size: 358 3149 210 243 510
Fragments arranged by size:
3149 510 358 243 210
XhoI C'TCGA_G
Cuts at: 0 167 4470
Size: 167 4303
XmnI GAAnn'nnTTC
Cuts at: 0 1838 4470
Size: 1838 2632
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PsiI PspXI PstI
PvuI PvuII SacI SapI ScaI SexAI SfcI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BbeI BglII BlpI BmtI BplI BsaAI BsaHI BsiWI
BspHI BsrFI BssHII BstBI BstEII BstXI BstZ17I BtgZI
DraI EagI EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PshAI PspOMI PsrI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SwaI XbaI XmaI ZraI