Restriction map for
pFN21AA0528
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AviII BalI BamHI
BbrPI BbvCI BcgI BclI BglI
BsaAI BsaI BsgI BsiEI BsiWI
BsmBI BsmFI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FauI FbaI FseI FspI
HpaI KpnI KspI MluI MluNI
MscI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 2576 TCCGGA T^CCGGA
AciI 1297 GCGG CCGC(-3/-1)
AcyI 35 GGCGCC G[GA]^CG[CT]C
AflII 130 CTTAAG C^TTAAG
Alw44I 1106 GTGCAC G^TGCAC
AlwNI 1295 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 985 GGGCCC GGGCC^C
ApaLI 1106 GTGCAC G^TGCAC
AseI 2349 ATTAAT AT^TAAT
AsnI 2349 ATTAAT AT^TAAT
Asp700I 2347 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1160 CTCGAG C^[CT]CG[GA]G
AvrII 1942 CCTAGG C^CTAGG
BbeI 35 GGCGCC GGCGC^C
BfrI 130 CTTAAG C^TTAAG
BglII 1966 AGATCT A^GATCT
BlnI 1942 CCTAGG C^CTAGG
BlpI 1730 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1730 GCTTAGC GC^T[ACGT]AGC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
BsaWI 2576 TCCGGA [AT]^CCGG[AT]
BseAI 2576 TCCGGA T^CCGGA
BsoBI 1160 CTCGAG C^[CT]CG[GA]G
BspDI 404 ATCGAT AT^CGAT
BspEI 2576 TCCGGA T^CCGGA
BspLU11I 2687 ACATGT A^CATGT
BstAPI 244 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 708 GCGAGC GC[ACGT]^[ACGT]GC
CelII 1730 GCTTAGC GC^T[ACGT]AGC
CfoI 36 GCGC GCG^C
ClaI 404 ATCGAT AT^CGAT
EaeI 29 TGGCCG [CT]^GGCC[GA]
EcoNI 2098 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1905 GATATC GAT^ATC
EcoT22I 1166 ATGCAT ATGCA^T
HaeII 35 GGCGCC [GA]GCGC^[CT]
HhaI 36 GCGC GCG^C
Hin1I 35 GGCGCC G[GA]^CG[CT]C
HinP1I 36 GCGC G^CGC
HincII 902 GTCGAC GT[CT]^[GA]AC
HindII 902 GTCGAC GT[CT]^[GA]AC
HindIII 1120 AAGCTT A^AGCTT
KasI 35 GGCGCC G^GCGCC
MfeI 1862 CAATTG C^AATTG
MroI 2576 TCCGGA T^CCGGA
MslI 1743 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1295 CAGCGG C[AC]G^C[GT]G
MunI 1862 CAATTG C^AATTG
NarI 35 GGCGCC GG^CGCC
NcoI 2060 CCATGG C^CATGG
NsiI 1166 ATGCAT ATGCA^T
PaeR7I 1160 CTCGAG C^TCGAG
PflMI 1768 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 843 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2349 ATTAAT AT^TAAT
SacI 2783 GAGCTC GAGCT^C
SalI 902 GTCGAC G^TCGAC
ScaI 630 AGTACT AGT^ACT
SfoI 35 GGCGCC GGC^GCC
SwaI 398 ATTTAAAT ATTT^AAAT
Van91I 1768 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1160 CTCGAG C^TCGAG
XmnI 2347 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 382 GTAGAC GT^[AC][GT]AC
902 GTCGAC
AspHI 1106 GTGCAC G[AT]GC[AT]^C
2783 GAGCTC
BanI 35 GGCGCC G^G[CT][GA]CC
1330 GGCACC
BanII 985 GGGCCC G[GA]GC[CT]^C
2783 GAGCTC
BciVI 350 GTATCC GTATCC(6/5)
1133 GTATCC
BcnI 32 CCGGG CC^[GC]GG
2137 CCCGG
BseRI 2096 CTCCTC GAGGAG(10/8)
2729 GAGGAG
BsiHKAI 1106 GTGCAC G[AT]GC[AT]^C
2783 GAGCTC
Bsp1407I 266 TGTACA T^GTACA
1083 TGTACA
BsrDI 2435 CATTGC GCAATG(2/0)
2773 GCAATG
BsrGI 266 TGTACA T^GTACA
1083 TGTACA
BstYI 816 AGATCC [GA]^GATC[CT]
1966 AGATCT
DraIII 1379 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2594 CACCTTGTG
EcoRI 542 GAATTC G^AATTC
1694 GAATTC
HgaI 2899 GACGC GACGC(5/10)
1735 GCGTC
MflI 816 AGATCC [GA]^GATC[CT]
1966 AGATCT
NciI 32 CCGGG CC^[GC]GG
2137 CCCGG
SspBI 266 TGTACA T^GTACA
1083 TGTACA
XcmI 520 CCAGAATATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
944 CCAAAACTGGAATGG
XhoII 816 AGATCC [GA]^GATC[CT]
1966 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MnlI
MseI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I