(Circular) (Six-base) MAPSORT of: pFN21AA0528.seq Check: 4799 from: 1 to: 7705 pFN21AA0528 7705 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5515 278 Size: 53 83 186 936 3979 2468 Fragments arranged by size: 3979 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 2908 5048 1937 Size: 451 520 2140 4594 Fragments arranged by size: 4594 2140 520 451 Acc65I G'GTAC_C Cuts at: 5026 5026 Size: 7705 AclI AA'CG_TT Cuts at: 1563 5832 6205 1563 Size: 4269 373 3063 Fragments arranged by size: 4269 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 3936 4092 4167 4365 4788 5007 5768 Size: 407 1897 156 75 198 423 219 761 Cuts at: 5768 7152 7420 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1897 1384 761 423 407 268 219 198 156 75 AflII C'TTAA_G Cuts at: 820 1017 2135 820 Size: 197 1118 6390 Fragments arranged by size: 6390 1118 197 AflIII A'CryG_T Cuts at: 1203 3091 3383 4692 6604 7581 1203 Size: 1888 292 1309 1912 977 1327 Fragments arranged by size: 1912 1888 1327 1309 977 292 AgeI A'CCGG_T Cuts at: 6536 6536 Size: 7705 AhdI GACnn_n'nnGTC Cuts at: 6434 6434 Size: 7705 AleI CACnn'nnGTG Cuts at: 1166 3752 1166 Size: 2586 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7705 AlwNI CAG_nnn'CTG Cuts at: 3305 5448 7020 3305 Size: 2143 1572 3990 Fragments arranged by size: 3990 2143 1572 ApaI G_GGCC'C Cuts at: 2994 2994 Size: 7705 ApaLI G'TGCA_C Cuts at: 3111 5762 6918 3111 Size: 2651 1156 3898 Fragments arranged by size: 3898 2651 1156 ApoI r'AATT_y Cuts at: 1493 2099 2174 2438 2547 3044 3373 3699 4261 Size: 606 75 264 109 497 329 326 562 Cuts at: 4261 4324 4576 4609 5014 5240 5392 7501 1493 Size: 63 252 33 405 226 152 2109 1697 Fragments arranged by size: 2109 1697 606 562 497 405 329 326 264 252 226 152 109 75 63 33 AseI AT'TA_AT Cuts at: 160 1032 4355 6259 160 Size: 872 3323 1904 1606 Fragments arranged by size: 3323 1904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7705 AvaI C'yCGr_G Cuts at: 1870 1943 3165 5030 1870 Size: 73 1222 1865 4545 Fragments arranged by size: 4545 1865 1222 73 AvrII C'CTAG_G Cuts at: 3947 3947 Size: 7705 BamHI G'GATC_C Cuts at: 5035 5402 5035 Size: 367 7338 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 3335 5026 6481 618 Size: 325 217 627 253 1295 1691 1455 1842 Fragments arranged by size: 1842 1691 1455 1295 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2994 4792 5024 1391 Size: 1603 1798 232 4072 Fragments arranged by size: 4072 1798 1603 232 BbeI G_GCGC'C Cuts at: 2044 2044 Size: 7705 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 2526 3809 4840 928 Size: 971 300 327 1283 1031 3793 Fragments arranged by size: 3793 1283 1031 971 327 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5896 5930 1903 Size: 34 50 34 3875 34 3678 Fragments arranged by size: 3875 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2366 3149 5597 6807 2366 Size: 783 2448 1210 3264 Fragments arranged by size: 3264 2448 1210 783 BclI T'GATC_A Cuts at: 1548 1548 Size: 7705 BfrBI ATG'CAT Cuts at: 3173 3173 Size: 7705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6316 136 Size: 107 122 71 631 605 4644 1525 Fragments arranged by size: 4644 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3971 7700 3971 Size: 3729 3976 BlpI GC'TnA_GC Cuts at: 3736 5122 3736 Size: 1386 6319 Bme1580I G_kGCm'C Cuts at: 1790 2994 3115 5766 6922 1790 Size: 1204 121 2651 1156 2573 Fragments arranged by size: 2651 2573 1204 1156 121 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7705 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5436 6394 442 Size: 416 53 83 399 24 4019 958 1753 Fragments arranged by size: 4019 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7705 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 3888 4677 6365 1239 Size: 1469 1180 789 1688 2579 Fragments arranged by size: 2579 1688 1469 1180 789 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4253 5830 6695 6993 3943 Size: 310 1577 865 298 4655 Fragments arranged by size: 4655 1577 865 310 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6368 882 Size: 617 4869 2219 Fragments arranged by size: 4869 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5496 493 Size: 711 4292 2702 Fragments arranged by size: 4292 2702 711 BsaBI GATnn'nnATC Cuts at: 2283 2406 2826 3976 7699 2283 Size: 123 420 1150 3723 2289 Fragments arranged by size: 3723 2289 1150 420 123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 5512 5894 6529 Size: 53 83 186 936 508 3471 382 635 Cuts at: 6529 275 Size: 1451 Fragments arranged by size: 3471 1451 936 635 508 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 6138 6536 6810 6957 7368 4581 Size: 1557 398 274 147 411 4918 Fragments arranged by size: 4918 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 1178 Size: 30 165 30 1278 30 6172 Fragments arranged by size: 6172 1278 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4092 4749 1186 Size: 2906 657 4142 Fragments arranged by size: 4142 2906 657 BseYI C'CCAG_C Cuts at: 2755 3797 6908 7404 2755 Size: 1042 3111 496 3056 Fragments arranged by size: 3111 3056 1042 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5155 5916 6944 1540 Size: 275 186 3154 761 1028 2301 Fragments arranged by size: 3154 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3115 4792 5766 5851 6922 3115 Size: 1677 974 85 1071 3898 Fragments arranged by size: 3898 1677 1071 974 85 BsmI GAATG_Cn' Cuts at: 2762 3685 4819 5216 5309 2762 Size: 923 1134 397 93 5158 Fragments arranged by size: 5158 1134 923 397 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2994 3115 4792 5024 5766 5851 6922 Size: 399 1204 121 1677 232 742 85 1071 Cuts at: 6922 1391 Size: 2174 Fragments arranged by size: 2174 1677 1204 1071 742 399 232 121 85 BspEI T'CCGG_A Cuts at: 4581 7368 4581 Size: 2787 4918 BspHI T'CATG_A Cuts at: 5594 7324 5594 Size: 1730 5975 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5060 844 Size: 4216 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5592 1394 Size: 429 3769 3507 Fragments arranged by size: 3769 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4785 6200 6374 65 Size: 1183 3191 346 1415 174 1396 Fragments arranged by size: 3191 1415 1396 1183 346 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6349 6536 1700 Size: 110 4539 187 2869 Fragments arranged by size: 4539 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 3088 96 Size: 2175 817 4713 Fragments arranged by size: 4713 2175 817 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7705 BssSI C'ACGA_G Cuts at: 5765 6777 5765 Size: 1012 6693 BstAPI GCAn_nnn'nTGC Cuts at: 794 2255 794 Size: 1461 6244 BstBI TT'CG_AA Cuts at: 6509 6509 Size: 7705 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7705 BstYI r'GATC_y Cuts at: 1972 2821 3971 5035 5402 5787 5804 7256 7342 Size: 849 1150 1064 367 385 17 1452 86 Cuts at: 7342 7354 7700 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1150 1064 849 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2359 3339 4065 513 Size: 552 1294 980 726 4153 Fragments arranged by size: 4153 1294 980 726 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6966 Fragments arranged by size: 6966 683 56 BtsI GCAGTG_nn' Cuts at: 4847 4914 5221 6015 6035 4847 Size: 67 307 794 20 6517 Fragments arranged by size: 6517 794 307 67 20 ClaI AT'CG_AT Cuts at: 1554 2410 1554 Size: 856 6849 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4545 4580 529 Size: 35 3981 35 3654 Fragments arranged by size: 3981 3654 35 35 DraI TTT'AAA Cuts at: 2119 2173 2406 2998 4285 5009 2119 Size: 54 233 592 1287 724 4815 Fragments arranged by size: 4815 1287 724 592 233 54 DraIII CAC_nnn'GTG Cuts at: 3389 4604 3389 Size: 1215 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6712 809 Size: 507 5396 1802 Fragments arranged by size: 5396 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 5152 6041 Size: 54 1425 50 275 18 204 3118 889 Cuts at: 6041 7670 8 Size: 1629 43 Fragments arranged by size: 3118 1629 1425 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5152 1537 Size: 275 3340 4090 Fragments arranged by size: 4090 3340 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 2923 3264 3912 4725 5635 1353 Size: 1046 524 341 648 813 910 3423 Fragments arranged by size: 3423 1046 910 813 648 524 341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6292 6666 6812 387 Size: 1064 138 4703 374 146 1280 Fragments arranged by size: 4703 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4790 4790 Size: 7705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2708 3888 3936 4092 4167 4365 Size: 393 407 669 1180 48 156 75 198 Cuts at: 4365 4677 4788 5007 5768 6365 7152 7420 1239 Size: 312 111 219 761 597 787 268 1524 Fragments arranged by size: 1524 1180 787 761 669 597 407 393 312 268 219 198 156 111 75 48 EcoNI CCTnn'n_nnAGG Cuts at: 4107 4107 Size: 7705 EcoO109I rG'GnC_Cy Cuts at: 2849 2990 2991 2849 Size: 141 1 7563 Fragments arranged by size: 7563 141 1 EcoRI G'AATT_C Cuts at: 2547 3699 5014 5392 2547 Size: 1152 1315 378 4860 Fragments arranged by size: 4860 1315 1152 378 EcoRV GAT'ATC Cuts at: 3912 3912 Size: 7705 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2644 3557 2644 Size: 913 6792 FspI TGC'GCA Cuts at: 6211 6211 Size: 7705 HaeII r_GCGC'y Cuts at: 1678 2044 6852 1678 Size: 366 4808 2531 Fragments arranged by size: 4808 2531 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2571 2603 2681 2713 3921 Size: 32 135 32 999 32 78 32 1208 Cuts at: 3921 3953 4284 4316 4624 4656 4702 4734 6346 Size: 32 331 32 308 32 46 32 1612 Cuts at: 6346 6378 6420 6452 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1612 1208 999 331 308 135 78 46 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2909 5049 5295 669 Size: 988 281 971 2140 246 3079 Fragments arranged by size: 3079 2140 988 971 281 246 HindIII A'AGCT_T Cuts at: 748 3125 748 Size: 2377 5328 HpaI GTT'AAC Cuts at: 5295 5295 Size: 7705 KasI G'GCGC_C Cuts at: 2040 2040 Size: 7705 KpnI G_GTAC'C Cuts at: 5030 5030 Size: 7705 MfeI C'AATT_G Cuts at: 3867 5304 3867 Size: 1437 6268 MluI A'CGCG_T Cuts at: 7581 7581 Size: 7705 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 6819 7003 1305 Size: 1707 3807 184 2007 Fragments arranged by size: 3807 2007 1707 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5883 Fragments arranged by size: 5883 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3752 5663 6022 6181 518 Size: 648 2586 1911 359 159 2042 Fragments arranged by size: 2586 2042 1911 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3302 5121 5798 6946 7191 1606 Size: 1696 1819 677 1148 245 2120 Fragments arranged by size: 2120 1819 1696 1148 677 245 NarI GG'CG_CC Cuts at: 2041 2041 Size: 7705 NcoI C'CATG_G Cuts at: 513 1065 4065 513 Size: 552 3000 4153 Fragments arranged by size: 4153 3000 552 NdeI CA'TA_TG Cuts at: 387 3409 3823 3843 387 Size: 3022 414 20 4249 Fragments arranged by size: 4249 3022 414 20 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7705 NotI GC'GGCC_GC Cuts at: 5152 5152 Size: 7705 NsiI A_TGCA'T Cuts at: 3175 3175 Size: 7705 NspI r_CATG'y Cuts at: 1132 1229 2778 3212 4696 5063 6608 1132 Size: 97 1549 434 1484 367 1545 2229 Fragments arranged by size: 2229 1549 1545 1484 434 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7705 PciI A'CATG_T Cuts at: 4692 6604 4692 Size: 1912 5793 PflMI CCAn_nnn'nTGG Cuts at: 1108 3779 1108 Size: 2671 5034 PfoI T'CCnGG_A Cuts at: 3076 4654 3076 Size: 1578 6127 PmeI GTTT'AAAC Cuts at: 5009 5009 Size: 7705 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5774 5806 7287 7319 5774 Size: 32 1481 32 6160 Fragments arranged by size: 6160 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2849 2849 Size: 7705 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7705 PsiI TTA'TAA Cuts at: 4889 5275 4889 Size: 386 7319 PspOMI G'GGCC_C Cuts at: 2990 2990 Size: 7705 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2716 2748 2716 Size: 32 7673 PstI C_TGCA'G Cuts at: 830 2616 3330 3464 5057 830 Size: 1786 714 134 1593 3478 Fragments arranged by size: 3478 1786 1593 714 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7705 SacI G_AGCT'C Cuts at: 4792 4792 Size: 7705 SalI G'TCGA_C Cuts at: 1936 2907 5047 1936 Size: 971 2140 4594 Fragments arranged by size: 4594 2140 971 SbfI CC_TGCA'GG Cuts at: 5057 5057 Size: 7705 ScaI AGT'ACT Cuts at: 2637 5953 7665 2637 Size: 3316 1712 2677 Fragments arranged by size: 3316 2677 1712 SfcI C'TryA_G Cuts at: 826 1045 2470 2612 2902 3234 3244 3326 3460 Size: 219 1425 142 290 332 10 82 134 Cuts at: 3460 3498 3597 5053 6188 6869 7060 826 Size: 38 99 1456 1135 681 191 1471 Fragments arranged by size: 1471 1456 1425 1135 681 332 290 219 191 142 134 99 82 38 10 SfoI GGC'GCC Cuts at: 2042 2042 Size: 7705 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7705 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7705 SmaI CCC'GGG Cuts at: 1872 5032 1872 Size: 3160 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 3165 3958 4268 5809 6710 Size: 197 926 192 1030 793 310 1541 901 Cuts at: 6710 6972 820 Size: 262 1553 Fragments arranged by size: 1553 1541 1030 926 901 793 310 262 197 192 SnaBI TAC'GTA Cuts at: 493 5496 493 Size: 5003 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7705 SphI G_CATG'C Cuts at: 1132 5063 1132 Size: 3931 3774 SspI AAT'ATT Cuts at: 5 52 5629 5 Size: 47 5577 2081 Fragments arranged by size: 5577 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7705 StyI C'CwwG_G Cuts at: 513 1065 2558 3947 4065 5144 513 Size: 552 1493 1389 118 1079 3074 Fragments arranged by size: 3074 1493 1389 1079 552 118 SwaI ATTT'AAAT Cuts at: 2406 2406 Size: 7705 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2962 3704 3949 4556 5732 5749 5902 Size: 1255 1382 742 245 607 1176 17 153 Cuts at: 5902 6087 6532 325 Size: 185 445 1498 Fragments arranged by size: 1498 1382 1255 1176 742 607 445 245 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 2635 2732 Size: 274 80 33 51 1443 294 364 97 Cuts at: 2732 3088 3307 4278 4982 5951 7663 96 Size: 356 219 971 704 969 1712 138 Fragments arranged by size: 1712 1443 971 969 704 364 356 294 274 219 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7665 7697 7665 Size: 32 7673 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7705 XbaI T'CTAG_A Cuts at: 5041 5041 Size: 7705 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2532 2956 2532 Size: 424 7281 XhoI C'TCGA_G Cuts at: 1943 3165 1943 Size: 1222 6483 XmaI C'CCGG_G Cuts at: 1870 5030 1870 Size: 3160 4545 XmnI GAAnn'nnTTC Cuts at: 4356 5834 4356 Size: 1478 6227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5513 276 Size: 53 83 186 936 3979 2468 Fragments arranged by size: 3979 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI