Restriction map for
pFN21AA0528
(Linear) (Six-base) MAPSORT of: KIAA0528.seq Check: 3493 from: 1 to: 3000
KIAA0528.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 383 903 3000
Size: 383 520 2097
Fragments arranged by size:
2097 520 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1931 2087 2162 2360 2783 3000
Size: 34 1897 156 75 198 423 217
Fragments arranged by size:
1897 423 217 198 156 75 34
AflII C'TTAA_G
Cuts at: 0 130 3000
Size: 130 2870
AflIII A'CryG_T
Cuts at: 0 1086 1378 2687 3000
Size: 1086 292 1309 313
Fragments arranged by size:
1309 1086 313 292
AleI CACnn'nnGTG
Cuts at: 0 1747 3000
Size: 1747 1253
AlwNI CAG_nnn'CTG
Cuts at: 0 1300 3000
Size: 1300 1700
ApaI G_GGCC'C
Cuts at: 0 989 3000
Size: 989 2011
ApaLI G'TGCA_C
Cuts at: 0 1106 3000
Size: 1106 1894
ApoI r'AATT_y
Cuts at: 0 94 169 433 542 1039 1368 1694 2256
Size: 94 75 264 109 497 329 326 562
Cuts at: 2256 2319 2571 2604 3000
Size: 63 252 33 396
Fragments arranged by size:
562 497 396 329 326 264 252 109
94 75 63 33
AseI AT'TA_AT
Cuts at: 0 2350 3000
Size: 2350 650
AvaI C'yCGr_G
Cuts at: 0 1160 3000
Size: 1160 1840
AvrII C'CTAG_G
Cuts at: 0 1942 3000
Size: 1942 1058
BanI G'GyrC_C
Cuts at: 0 35 1330 3000
Size: 35 1295 1670
Fragments arranged by size:
1670 1295 35
BanII G_rGCy'C
Cuts at: 0 989 2787 3000
Size: 989 1798 213
Fragments arranged by size:
1798 989 213
BbeI G_GCGC'C
Cuts at: 0 39 3000
Size: 39 2961
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 521 1804 2835 3000
Size: 194 327 1283 1031 165
Fragments arranged by size:
1283 1031 327 194 165
BciVI GTATCCnnnnn_n'
Cuts at: 0 361 1144 3000
Size: 361 783 1856
Fragments arranged by size:
1856 783 361
BfrBI ATG'CAT
Cuts at: 0 1168 3000
Size: 1168 1832
BglII A'GATC_T
Cuts at: 0 1966 3000
Size: 1966 1034
BlpI GC'TnA_GC
Cuts at: 0 1731 3000
Size: 1731 1269
Bme1580I G_kGCm'C
Cuts at: 0 989 1110 3000
Size: 989 121 1890
Fragments arranged by size:
1890 989 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1883 2672 3000
Size: 703 1180 789 328
Fragments arranged by size:
1180 789 703 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1938 2248 3000
Size: 1938 310 752
Fragments arranged by size:
1938 752 310
BsaBI GATnn'nnATC
Cuts at: 0 278 401 821 1971 3000
Size: 278 123 420 1150 1029
Fragments arranged by size:
1150 1029 420 278 123
BsaHI Gr'CG_yC
Cuts at: 0 36 3000
Size: 36 2964
BsaWI w'CCGG_w
Cuts at: 0 2576 3000
Size: 2576 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 3000
Size: 676 30 2294
Fragments arranged by size:
2294 676 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2087 2744 3000
Size: 2087 657 256
Fragments arranged by size:
2087 657 256
BseYI C'CCAG_C
Cuts at: 0 750 1792 3000
Size: 750 1042 1208
Fragments arranged by size:
1208 1042 750
BsiHKAI G_wGCw'C
Cuts at: 0 1110 2787 3000
Size: 1110 1677 213
Fragments arranged by size:
1677 1110 213
BsmI GAATG_Cn'
Cuts at: 0 757 1680 2814 3000
Size: 757 923 1134 186
Fragments arranged by size:
1134 923 757 186
Bsp1286I G_dGCh'C
Cuts at: 0 989 1110 2787 3000
Size: 989 121 1677 213
Fragments arranged by size:
1677 989 213 121
BspEI T'CCGG_A
Cuts at: 0 2576 3000
Size: 2576 424
BsrDI GCAATG_nn'
Cuts at: 0 2434 2780 3000
Size: 2434 346 220
Fragments arranged by size:
2434 346 220
BsrGI T'GTAC_A
Cuts at: 0 266 1083 3000
Size: 266 817 1917
Fragments arranged by size:
1917 817 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 250 3000
Size: 250 2750
BstYI r'GATC_y
Cuts at: 0 816 1966 3000
Size: 816 1150 1034
Fragments arranged by size:
1150 1034 816
BtgI C'CryG_G
Cuts at: 0 354 1334 2060 3000
Size: 354 980 726 940
Fragments arranged by size:
980 940 726 354
BtsI GCAGTG_nn'
Cuts at: 0 2842 2909 3000
Size: 2842 67 91
Fragments arranged by size:
2842 91 67
ClaI AT'CG_AT
Cuts at: 0 405 3000
Size: 405 2595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2540 2575 3000
Size: 2540 35 425
Fragments arranged by size:
2540 425 35
DraI TTT'AAA
Cuts at: 0 114 168 401 993 2280 3000
Size: 114 54 233 592 1287 720
Fragments arranged by size:
1287 720 592 233 114 54
DraIII CAC_nnn'GTG
Cuts at: 0 1384 2599 3000
Size: 1384 1215 401
Fragments arranged by size:
1384 1215 401
EaeI y'GGCC_r
Cuts at: 0 29 3000
Size: 29 2971
EarI CTCTTCn'nnn_
Cuts at: 0 394 918 1259 1907 2720 3000
Size: 394 524 341 648 813 280
Fragments arranged by size:
813 648 524 394 341 280
EcoICRI GAG'CTC
Cuts at: 0 2785 3000
Size: 2785 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 703 1883 1931 2087 2162 2360 2672
Size: 34 669 1180 48 156 75 198 312
Cuts at: 2672 2783 3000
Size: 111 217
Fragments arranged by size:
1180 669 312 217 198 156 111 75
48 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2102 3000
Size: 2102 898
EcoO109I rG'GnC_Cy
Cuts at: 0 844 985 986 3000
Size: 844 141 1 2014
Fragments arranged by size:
2014 844 141 1
EcoRI G'AATT_C
Cuts at: 0 542 1694 3000
Size: 542 1152 1306
Fragments arranged by size:
1306 1152 542
EcoRV GAT'ATC
Cuts at: 0 1907 3000
Size: 1907 1093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 639 1552 3000
Size: 639 913 1448
Fragments arranged by size:
1448 913 639
HaeII r_GCGC'y
Cuts at: 0 39 3000
Size: 39 2961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 566 598 676 708 1916 1948 2279 2311
Size: 566 32 78 32 1208 32 331 32
Cuts at: 2311 2619 2651 2697 2729 3000
Size: 308 32 46 32 271
Fragments arranged by size:
1208 566 331 308 271 78 46 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 904 3000
Size: 904 2096
HindIII A'AGCT_T
Cuts at: 0 1120 3000
Size: 1120 1880
KasI G'GCGC_C
Cuts at: 0 35 3000
Size: 35 2965
MfeI C'AATT_G
Cuts at: 0 1862 3000
Size: 1862 1138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 3000
Size: 1007 1993
MslI CAynn'nnrTG
Cuts at: 0 1747 3000
Size: 1747 1253
MspA1I CmG'CkG
Cuts at: 0 1297 3000
Size: 1297 1703
NarI GG'CG_CC
Cuts at: 0 36 3000
Size: 36 2964
NcoI C'CATG_G
Cuts at: 0 2060 3000
Size: 2060 940
NdeI CA'TA_TG
Cuts at: 0 1404 1818 1838 3000
Size: 1404 414 20 1162
Fragments arranged by size:
1404 1162 414 20
NsiI A_TGCA'T
Cuts at: 0 1170 3000
Size: 1170 1830
NspI r_CATG'y
Cuts at: 0 773 1207 2691 3000
Size: 773 434 1484 309
Fragments arranged by size:
1484 773 434 309
PciI A'CATG_T
Cuts at: 0 2687 3000
Size: 2687 313
PflMI CCAn_nnn'nTGG
Cuts at: 0 1774 3000
Size: 1774 1226
PfoI T'CCnGG_A
Cuts at: 0 1071 2649 3000
Size: 1071 1578 351
Fragments arranged by size:
1578 1071 351
PpuMI rG'GwC_Cy
Cuts at: 0 844 3000
Size: 844 2156
PsiI TTA'TAA
Cuts at: 0 2884 3000
Size: 2884 116
PspOMI G'GGCC_C
Cuts at: 0 985 3000
Size: 985 2015
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 711 743 3000
Size: 711 32 2257
Fragments arranged by size:
2257 711 32
PstI C_TGCA'G
Cuts at: 0 611 1325 1459 3000
Size: 611 714 134 1541
Fragments arranged by size:
1541 714 611 134
SacI G_AGCT'C
Cuts at: 0 2787 3000
Size: 2787 213
SalI G'TCGA_C
Cuts at: 0 902 3000
Size: 902 2098
ScaI AGT'ACT
Cuts at: 0 632 3000
Size: 632 2368
SfcI C'TryA_G
Cuts at: 0 465 607 897 1229 1239 1321 1455 1493
Size: 465 142 290 332 10 82 134 38
Cuts at: 1493 1592 3000
Size: 99 1408
Fragments arranged by size:
1408 465 332 290 142 134 99 82
38 10
SfoI GGC'GCC
Cuts at: 0 37 3000
Size: 37 2963
SmlI C'TyrA_G
Cuts at: 0 130 1160 1953 2263 3000
Size: 130 1030 793 310 737
Fragments arranged by size:
1030 793 737 310 130
StyI C'CwwG_G
Cuts at: 0 553 1942 2060 3000
Size: 553 1389 118 940
Fragments arranged by size:
1389 940 553 118
SwaI ATTT'AAAT
Cuts at: 0 401 3000
Size: 401 2599
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 957 1699 1944 2551 3000
Size: 957 742 245 607 449
Fragments arranged by size:
957 742 607 449 245
TatI w'GTAC_w
Cuts at: 0 266 630 727 1083 1302 2273 2977 3000
Size: 266 364 97 356 219 971 704 23
Fragments arranged by size:
971 704 364 356 266 219 97 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 527 951 3000
Size: 527 424 2049
Fragments arranged by size:
2049 527 424
XhoI C'TCGA_G
Cuts at: 0 1160 3000
Size: 1160 1840
XmnI GAAnn'nnTTC
Cuts at: 0 2351 3000
Size: 2351 649
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BanI BanII BbeI BbsI
BciVI BfrBI BglII BlpI Bme1580I BpmI BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BsrDI BsrGI BstAPI BstYI BtgI BtsI ClaI
CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MslI MspA1I NarI NcoI NdeI
NsiI NspI PciI PflMI PfoI PpuMI PsiI PspOMI
PsrI PstI SacI SalI ScaI SfcI SfoI SmlI
StyI SwaI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AscI AsiSI BaeI BamHI BbvCI BcgI BclI
BglI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsgI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
DrdI EagI EciI FseI FspI FspAI HpaI KpnI
MluI MscI NaeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PshAI PspXI PvuI PvuII
RsrII SacII SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
TstI Tth111I XbaI XmaI ZraI