(Circular) (Six-base) MAPSORT of: pFN21AA0523.seq Check: 1746 from: 1 to: 6430 pFN21AA0523 6430 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 2994 3773 1937 Size: 619 438 779 4594 Fragments arranged by size: 4594 779 619 438 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2434 4493 5877 6145 1632 Size: 802 2059 1384 268 1917 Fragments arranged by size: 2059 1917 1384 802 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 3096 3531 5329 6306 1203 Size: 1893 435 1798 977 1327 Fragments arranged by size: 1893 1798 1327 977 435 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 3240 1166 Size: 2074 4356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2117 2234 3512 1184 Size: 933 117 1278 4102 Fragments arranged by size: 4102 1278 933 117 AlwNI CAG_nnn'CTG Cuts at: 2056 2880 2974 4173 5745 2056 Size: 824 94 1199 1572 2741 Fragments arranged by size: 2741 1572 1199 824 94 ApaI G_GGCC'C Cuts at: 2141 2399 2141 Size: 258 6172 ApaLI G'TGCA_C Cuts at: 3467 4487 5643 3467 Size: 1020 1156 4254 Fragments arranged by size: 4254 1156 1020 ApoI r'AATT_y Cuts at: 1493 3317 3739 3965 4117 6226 1493 Size: 1824 422 226 152 2109 1697 Fragments arranged by size: 2109 1824 1697 422 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2142 2229 2399 2833 3755 1870 Size: 73 199 87 170 434 922 4545 Fragments arranged by size: 4545 922 434 199 170 87 73 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2432 3108 3339 3496 Size: 325 217 627 359 286 676 231 157 Cuts at: 3496 3751 5206 618 Size: 255 1455 1842 Fragments arranged by size: 1842 1455 676 627 359 325 286 255 231 217 157 BanII G_rGCy'C Cuts at: 1391 2141 2218 2276 2399 2663 2723 3624 3749 Size: 750 77 58 123 264 60 901 125 Cuts at: 3749 1391 Size: 4072 Fragments arranged by size: 4072 901 750 264 125 123 77 60 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5459 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2210 2244 2999 3033 4621 4655 1903 Size: 34 273 34 755 34 1588 34 3678 Fragments arranged by size: 3678 1588 755 273 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 3648 1548 Size: 2100 4330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2183 5041 136 Size: 107 122 71 631 605 511 2858 1525 Fragments arranged by size: 2858 1525 631 605 511 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2541 3847 2541 Size: 1306 5124 Bme1580I G_kGCm'C Cuts at: 1790 2141 2149 2399 3106 3342 3471 3501 3726 Size: 351 8 250 707 236 129 30 225 Cuts at: 3726 4491 5647 1790 Size: 765 1156 2573 Fragments arranged by size: 2573 1156 765 707 351 250 236 225 129 30 8 BmgBI CAC'GTC Cuts at: 1318 3427 1318 Size: 2109 4321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4161 5119 442 Size: 416 53 83 399 24 2744 958 1753 Fragments arranged by size: 2744 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2677 2677 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2012 2707 5090 1239 Size: 773 695 2383 2579 Fragments arranged by size: 2579 2383 773 695 Bpu10I CC'TnA_GC Cuts at: 3618 3618 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2521 3425 4555 5420 5718 2521 Size: 904 1130 865 298 3233 Fragments arranged by size: 3233 1130 904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5093 882 Size: 617 3594 2219 Fragments arranged by size: 3594 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3099 4221 493 Size: 711 1895 1122 2702 Fragments arranged by size: 2702 1895 1122 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4237 4619 5254 275 Size: 53 83 186 936 2704 382 635 1451 Fragments arranged by size: 2704 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2310 3288 3663 4863 5261 5535 5682 6093 2310 Size: 978 375 1200 398 274 147 411 2647 Fragments arranged by size: 2647 1200 978 411 398 375 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 4906 Fragments arranged by size: 4906 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3491 3560 1186 Size: 2305 69 4056 Fragments arranged by size: 4056 2305 69 BseYI C'CCAG_C Cuts at: 2188 2251 2792 2880 5633 6129 2188 Size: 63 541 88 2753 496 2489 Fragments arranged by size: 2753 2489 541 496 88 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 1163 Size: 573 468 5389 Fragments arranged by size: 5389 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 3880 4641 5669 1540 Size: 275 186 1600 279 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 279 275 186 BsiHKAI G_wGCw'C Cuts at: 3471 4491 4576 5647 3471 Size: 1020 85 1071 4254 Fragments arranged by size: 4254 1071 1020 85 BsmI GAATG_Cn' Cuts at: 2891 3941 4034 2891 Size: 1050 93 5287 Fragments arranged by size: 5287 1050 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2149 2218 2276 2399 2663 2723 Size: 399 351 8 69 58 123 264 60 Cuts at: 2723 3106 3342 3471 3501 3624 3726 3749 4491 Size: 383 236 129 30 123 102 23 742 Cuts at: 4491 4576 5647 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 383 351 264 236 129 123 123 102 85 69 60 58 30 23 8 BspEI T'CCGG_A Cuts at: 2310 3288 3663 6093 2310 Size: 978 375 2430 2647 Fragments arranged by size: 2647 2430 978 375 BspHI T'CATG_A Cuts at: 2364 4319 6049 2364 Size: 1955 1730 2745 Fragments arranged by size: 2745 1955 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2928 3785 844 Size: 2084 857 3489 Fragments arranged by size: 3489 2084 857 BsrBI CCG'CTC Cuts at: 1394 1823 2545 3550 4317 1394 Size: 429 722 1005 767 3507 Fragments arranged by size: 3507 1005 767 722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 2561 2598 2682 3600 5074 5261 Size: 110 280 471 37 84 918 1474 187 Cuts at: 5261 1700 Size: 2869 Fragments arranged by size: 2869 1474 918 471 280 187 110 84 37 BsrGI T'GTAC_A Cuts at: 96 3023 96 Size: 2927 3503 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 2465 3236 3409 4490 5502 2465 Size: 771 173 1081 1012 3393 Fragments arranged by size: 3393 1081 1012 771 173 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 5057 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 3242 3242 Size: 6430 BstYI r'GATC_y Cuts at: 1972 3760 4127 4512 4529 5981 6067 6079 6425 Size: 1788 367 385 17 1452 86 12 346 Cuts at: 6425 1972 Size: 1977 Fragments arranged by size: 1977 1788 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2961 2961 Size: 6430 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2749 2820 2931 513 Size: 552 939 177 568 71 111 4012 Fragments arranged by size: 4012 939 568 552 177 111 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 2521 3946 4740 4760 2521 Size: 1425 794 20 4191 Fragments arranged by size: 4191 1425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2231 5437 809 Size: 507 915 3206 1802 Fragments arranged by size: 3206 1802 915 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2175 2461 Size: 54 1425 50 275 18 177 168 286 Cuts at: 2461 2865 2950 3383 3598 3877 4766 6395 8 Size: 404 85 433 215 279 889 1629 43 Fragments arranged by size: 1629 1425 889 433 404 286 279 275 215 177 168 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 3877 1537 Size: 275 1786 279 4090 Fragments arranged by size: 4090 1786 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 3365 4360 1353 Size: 1269 743 995 3423 Fragments arranged by size: 3423 1269 995 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2594 2749 3220 5017 5391 Size: 1064 138 441 564 155 471 1797 374 Cuts at: 5391 5537 387 Size: 146 1280 Fragments arranged by size: 1797 1280 1064 564 471 441 374 155 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2434 2707 4493 5090 5877 6145 Size: 393 380 422 273 1786 597 787 268 Cuts at: 6145 1239 Size: 1524 Fragments arranged by size: 1786 1524 787 597 422 393 380 273 268 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2137 2138 2396 2956 3303 2137 Size: 1 258 560 347 5264 Fragments arranged by size: 5264 560 347 258 1 EcoRI G'AATT_C Cuts at: 3317 3739 4117 3317 Size: 422 378 5630 Fragments arranged by size: 5630 422 378 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2189 3607 5577 1678 Size: 511 1418 1970 2531 Fragments arranged by size: 2531 1970 1418 511 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 3204 3236 5071 Size: 32 135 32 1100 32 500 32 1835 Cuts at: 5071 5103 5145 5177 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1835 1100 500 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3397 3774 4020 669 Size: 988 281 1459 377 246 3079 Fragments arranged by size: 3079 1459 988 377 281 246 HindIII A'AGCT_T Cuts at: 748 3076 748 Size: 2328 4102 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 2785 5544 5728 1305 Size: 742 738 2759 184 2007 Fragments arranged by size: 2759 2007 742 738 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2463 2867 2952 10 Size: 54 1425 343 177 454 404 85 3488 Fragments arranged by size: 3488 1425 454 404 343 177 85 54 MslI CAynn'nnrTG Cuts at: 518 1166 3240 4388 4747 4906 518 Size: 648 2074 1148 359 159 2042 Fragments arranged by size: 2074 2042 1148 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2304 2349 2609 2751 3356 3846 4523 Size: 507 191 45 260 142 605 490 677 Cuts at: 4523 5671 5916 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 605 507 490 260 245 191 142 45 NaeI GCC'GGC Cuts at: 2563 3602 2563 Size: 1039 5391 NcoI C'CATG_G Cuts at: 513 1065 2004 2931 513 Size: 552 939 927 4012 Fragments arranged by size: 4012 939 927 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2561 3600 2561 Size: 1039 5391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2266 2786 3125 3788 5333 1132 Size: 97 1037 520 339 663 1545 2229 Fragments arranged by size: 2229 1545 1037 663 520 339 97 PasI CC'CwG_GG Cuts at: 1348 2377 2392 3711 1348 Size: 1029 15 1319 4067 Fragments arranged by size: 4067 1319 1029 15 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6430 PfoI T'CCnGG_A Cuts at: 2723 3429 2723 Size: 706 5724 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PmlI CAC'GTG Cuts at: 3099 3099 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2956 3303 2956 Size: 347 6083 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2137 2395 2137 Size: 258 6172 PspXI vC'TCGA_Gb Cuts at: 2229 2229 Size: 6430 PstI C_TGCA'G Cuts at: 830 2112 2163 2271 2715 3782 830 Size: 1282 51 108 444 1067 3478 Fragments arranged by size: 3478 1282 1067 444 108 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 3356 3356 Size: 6430 RsrII CG'GwC_CG Cuts at: 2736 2736 Size: 6430 SacII CC_GC'GG Cuts at: 2752 2752 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SapI GCTCTTCn'nnn_ Cuts at: 3365 3365 Size: 6430 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2108 2159 2267 2711 3778 4913 5594 Size: 219 1063 51 108 444 1067 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1067 1063 681 444 219 191 108 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 2144 3757 1872 Size: 272 1613 4545 Fragments arranged by size: 4545 1613 272 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 2500 3440 4534 5435 5697 Size: 197 926 286 271 940 1094 901 262 Cuts at: 5697 820 Size: 1553 Fragments arranged by size: 1553 1094 940 926 901 286 271 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3125 3788 1132 Size: 1993 663 3774 Fragments arranged by size: 3774 1993 663 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2004 2862 2931 3869 513 Size: 552 939 858 69 938 3074 Fragments arranged by size: 3074 939 938 858 552 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 4457 4474 4627 4812 5257 325 Size: 1255 663 2214 17 153 185 445 1498 Fragments arranged by size: 2214 1498 1255 663 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3023 4676 6388 Size: 274 80 33 51 1443 1046 1653 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 1712 1653 1443 1046 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2772 2804 6390 6422 2772 Size: 32 3586 32 2780 Fragments arranged by size: 3586 2780 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3392 1316 Size: 2076 4354 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2818 3061 2818 Size: 243 6187 XhoI C'TCGA_G Cuts at: 1943 2229 1943 Size: 286 6144 XmaI C'CCGG_G Cuts at: 1870 2142 3755 1870 Size: 272 1613 4545 Fragments arranged by size: 4545 1613 272 XmnI GAAnn'nnTTC Cuts at: 2855 4559 2855 Size: 1704 4726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PsrI SacI SanDI SexAI SfiI SfoI SrfI SwaI