Restriction map for
pFN21AA0476
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BclI BfrI BglII BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SacII SalI SfoI
SfuI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1474 GTAGAC GT^[AC][GT]AC
AccIII 2630 TCCGGA T^CCGGA
AclI 1078 AACGTT AA^CGTT
Alw44I 4213 GTGCAC G^TGCAC
ApaLI 4213 GTGCAC G^TGCAC
AviII 935 TGCGCA TGC^GCA
BbrPI 4333 CACGTG CAC^GTG
BbvCI 4410 GCTGAGG CCTCAGC(-5/-2)
BsaAI 4333 CACGTG [CT]AC^GT[GA]
BsaWI 2630 TCCGGA [AT]^CCGG[AT]
BseAI 2630 TCCGGA T^CCGGA
BsiEI 2350 CGGTCG CG[GA][CT]^CG
BspEI 2630 TCCGGA T^CCGGA
BssSI 1787 CTCGTG CACGAG(-5/-1)
DraIII 2600 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1939 GAATTC G^AATTC
EcoT22I 198 ATGCAT ATGCA^T
FspI 935 TGCGCA TGC^GCA
HincII 1795 GTTGAC GT[CT]^[GA]AC
HindII 1795 GTTGAC GT[CT]^[GA]AC
MroI 2630 TCCGGA T^CCGGA
MseI 4376 TTAA T^TAA
NsiI 198 ATGCAT ATGCA^T
PmaCI 4333 CACGTG CAC^GTG
PmlI 4333 CACGTG CAC^GTG
Psp1406I 1078 AACGTT AA^CGTT
SapI 1494 GCTCTTC GCTCTTC(1/4)
SbfI 1812 CCTGCAGG CCTGCA^GG
ScaI 2099 AGTACT AGT^ACT
SexAI 2601 ACCAGGT A^CC[AT]GGT
SfiI 2925 GGCCGGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4417 CACCGGCG C[GA]^CCGG[CT]G
SphI 468 GCATGC GCATG^C
Sse8387I 1812 CCTGCAGG CCTGCA^GG
Tru9I 4376 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 991 CGCG CG^CG
1690 CGCG
AcsI 1939 GAATTC [GA]^AATT[CT]
3452 AAATTC
AcyI 2609 GGCGTC G[GA]^CG[CT]C
2867 GACGCC
AocI 76 CCTGAGG CC^T[ACGT]AGG
1615 CCTGAGG
ApoI 1939 GAATTC [GA]^AATT[CT]
3452 AAATTC
BamHI 3313 GGATCC G^GATCC
3427 GGATCC
BcgI 2058 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3706 CGAAGGCTCTGC
BciVI 1159 GTATCC GTATCC(6/5)
1421 GTATCC
BsaHI 2609 GGCGTC G[GA]^CG[CT]C
2867 GACGCC
BsaI 1101 GAGACC GGTCTC(1/5)
2846 GAGACC
BsmBI 1023 CGTCTC CGTCTC(1/5)
1320 CGTCTC
BsmI 4483 GAATGC GAATGC(1/-1)
3224 GCATTC
BstUI 991 CGCG CG^CG
1690 CGCG
Bsu36I 76 CCTGAGG CC^T[ACGT]AGG
1615 CCTGAGG
Eco81I 76 CCTGAGG CC^T[ACGT]AGG
1615 CCTGAGG
EcoNI 606 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2173 CCTGTGCCAGG
Hin1I 2609 GGCGTC G[GA]^CG[CT]C
2867 GACGCC
MvnI 991 CGCG CG^CG
1690 CGCG
NaeI 539 GCCGGC GCC^GGC
941 GCCGGC
NcoI 716 CCATGG C^CATGG
3278 CCATGG
NgoMIV 539 GCCGGC G^CCGGC
941 GCCGGC
SmlI 1732 CTCAAG C^T[CT][GA]AG
2670 CTTGAG
StuI 2948 AGGCCT AGG^CCT
3954 AGGCCT
Tsp509I 1940 AATT ^AATT
3453 AATT
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI