(Circular) (Six-base) MAPSORT of: pFN21AA0476.seq Check: 7050 from: 1 to: 9193 pFN21AA0476 9193 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4729 6285 4729 Size: 1556 7637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7003 278 Size: 53 83 186 936 5467 2468 Fragments arranged by size: 5467 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3480 6536 1937 Size: 1543 3056 4594 Fragments arranged by size: 4594 3056 1543 Acc65I G'GTAC_C Cuts at: 6514 6514 Size: 9193 AclI AA'CG_TT Cuts at: 1563 3084 7320 7693 1563 Size: 1521 4236 373 3063 Fragments arranged by size: 4236 3063 1521 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4799 7256 8640 8908 1632 Size: 3167 2457 1384 268 1917 Fragments arranged by size: 3167 2457 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8996 AflIII A'CryG_T Cuts at: 1203 8092 9069 1203 Size: 6889 977 1327 Fragments arranged by size: 6889 1327 977 AgeI A'CCGG_T Cuts at: 8024 8024 Size: 9193 AhdI GACnn_n'nnGTC Cuts at: 7922 7922 Size: 9193 AleI CACnn'nnGTG Cuts at: 1166 2150 2630 2786 1166 Size: 984 480 156 7573 Fragments arranged by size: 7573 984 480 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2954 3137 4445 5879 1184 Size: 1770 183 1308 1434 4498 Fragments arranged by size: 4498 1770 1434 1308 183 AlwNI CAG_nnn'CTG Cuts at: 3208 3217 3312 3598 3874 4277 4381 6035 6152 Size: 9 95 286 276 403 104 1654 117 Cuts at: 6152 6936 8508 3208 Size: 784 1572 3893 Fragments arranged by size: 3893 1654 1572 784 403 286 276 117 104 95 9 ApaI G_GGCC'C Cuts at: 2115 2582 3224 3685 4174 4297 6258 2115 Size: 467 642 461 489 123 1961 5050 Fragments arranged by size: 5050 1961 642 489 467 461 123 ApaLI G'TGCA_C Cuts at: 6218 7250 8406 6218 Size: 1032 1156 7005 Fragments arranged by size: 7005 1156 1032 ApoI r'AATT_y Cuts at: 1493 3944 5457 6502 6728 6880 8989 1493 Size: 2451 1513 1045 226 152 2109 1697 Fragments arranged by size: 2451 2109 1697 1513 1045 226 152 AseI AT'TA_AT Cuts at: 160 1032 7747 160 Size: 872 6715 1606 Fragments arranged by size: 6715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9193 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2693 3837 4061 4368 4905 4974 Size: 73 474 276 1144 224 307 537 69 Cuts at: 4974 5304 6518 1870 Size: 330 1214 4545 Fragments arranged by size: 4545 1214 1144 537 474 330 307 276 224 73 69 BamHI G'GATC_C Cuts at: 5318 5432 6523 6890 5318 Size: 114 1091 367 7621 Fragments arranged by size: 7621 1091 367 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2822 3188 4299 4360 Size: 325 217 627 634 401 366 1111 61 Cuts at: 4360 5156 5177 5533 5651 6420 6514 7969 618 Size: 796 21 356 118 769 94 1455 1842 Fragments arranged by size: 1842 1455 1111 796 769 634 627 401 366 356 325 217 118 94 61 21 BanII G_rGCy'C Cuts at: 1391 2115 2142 2582 3224 3685 3793 4041 4174 Size: 724 27 440 642 461 108 248 133 Cuts at: 4174 4297 4782 5066 5339 5739 5810 6237 6258 Size: 123 485 284 273 400 71 427 21 Cuts at: 6258 6512 1391 Size: 254 4072 Fragments arranged by size: 4072 724 642 485 461 440 427 400 284 273 254 248 133 123 108 71 27 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2701 2767 3745 5285 5387 928 Size: 971 802 66 978 1540 102 4734 Fragments arranged by size: 4734 1540 978 971 802 102 66 BbvCI CC'TCA_GC Cuts at: 6416 6416 Size: 9193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4052 4086 5700 5734 7384 7418 1903 Size: 34 2115 34 1614 34 1650 34 3678 Fragments arranged by size: 3678 2115 1650 1614 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3175 3437 7085 8295 3175 Size: 262 3648 1210 4073 Fragments arranged by size: 4073 3648 1210 262 BclI T'GATC_A Cuts at: 1548 1548 Size: 9193 BfrBI ATG'CAT Cuts at: 2205 2205 Size: 9193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 4910 4937 Size: 107 122 71 631 605 354 2884 27 Cuts at: 4937 5061 7804 136 Size: 124 2743 1525 Fragments arranged by size: 2884 2743 1525 631 605 354 124 122 107 71 27 BglII A'GATC_T Cuts at: 9188 9188 Size: 9193 BlpI GC'TnA_GC Cuts at: 2136 2897 5071 5338 6610 2136 Size: 761 2174 267 1272 4719 Fragments arranged by size: 4719 2174 1272 761 267 Bme1580I G_kGCm'C Cuts at: 1790 2115 2173 2190 2424 2528 2582 2706 2772 Size: 325 58 17 234 104 54 124 66 Cuts at: 2772 2887 2923 3224 3359 3377 3406 3414 3685 Size: 115 36 301 135 18 29 8 271 Cuts at: 3685 4174 4297 4314 4542 4906 4915 4992 5086 Size: 489 123 17 228 364 9 77 94 Cuts at: 5086 5182 5536 5592 5757 6222 6258 7254 8410 Size: 96 354 56 165 465 36 996 1156 Cuts at: 8410 1790 Size: 2573 Fragments arranged by size: 2573 1156 996 489 465 364 354 325 301 271 234 228 165 135 124 123 115 104 96 94 77 66 58 56 54 36 36 29 18 17 17 9 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 2183 2505 Size: 416 53 83 399 24 683 83 322 Cuts at: 2505 2897 2933 3401 4096 5080 5619 5940 6924 Size: 392 36 468 695 984 539 321 984 Cuts at: 6924 7882 442 Size: 958 1753 Fragments arranged by size: 1753 984 984 958 695 683 539 468 416 399 392 322 321 83 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3928 3731 Size: 197 8996 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2394 3418 3659 3710 3832 4535 5067 5852 Size: 1155 1024 241 51 122 703 532 785 Cuts at: 5852 6127 6468 7853 1239 Size: 275 341 1385 2579 Fragments arranged by size: 2579 1385 1155 1024 785 703 532 341 275 241 122 51 Bpu10I CC'TnA_GC Cuts at: 3225 3993 4284 5018 5065 5241 5804 6416 3225 Size: 768 291 734 47 176 563 612 6002 Fragments arranged by size: 6002 768 734 612 563 291 176 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3722 4696 7318 8183 8481 3722 Size: 974 2622 865 298 4434 Fragments arranged by size: 4434 2622 974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3100 4845 7856 882 Size: 617 1601 1745 3011 2219 Fragments arranged by size: 3011 2219 1745 1601 617 BsaAI yAC'GTr Cuts at: 493 1204 6340 6984 493 Size: 711 5136 644 2702 Fragments arranged by size: 5136 2702 711 644 BsaBI GATnn'nnATC Cuts at: 9187 9187 Size: 9193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4615 4873 7000 7382 Size: 53 83 186 936 3082 258 2127 382 Cuts at: 7382 8017 275 Size: 635 1451 Fragments arranged by size: 3082 2127 1451 936 635 382 258 186 83 53 BsaWI w'CCGG_w Cuts at: 4635 7626 8024 8298 8445 8856 4635 Size: 2991 398 274 147 411 4972 Fragments arranged by size: 4972 2991 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3008 3038 4727 4757 5819 Size: 30 165 30 1605 30 1689 30 1062 Cuts at: 5819 5849 1178 Size: 30 4522 Fragments arranged by size: 4522 1689 1605 1062 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2520 2681 3140 3515 3638 3920 4034 Size: 841 493 161 459 375 123 282 114 Cuts at: 4034 4079 4209 5048 6425 1186 Size: 45 130 839 1377 3954 Fragments arranged by size: 3954 1377 841 839 493 459 375 282 161 130 123 114 45 BseYI C'CCAG_C Cuts at: 2051 2219 2317 2581 2650 4205 4306 4623 4835 Size: 168 98 264 69 1555 101 317 212 Cuts at: 4835 4855 5173 5266 5529 5633 5739 6129 8396 Size: 20 318 93 263 104 106 390 2267 Cuts at: 8396 8892 2051 Size: 496 2352 Fragments arranged by size: 2352 2267 1555 496 390 318 317 264 263 212 168 106 104 101 98 93 69 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2820 3143 3329 3860 4367 4394 5282 Size: 573 1084 323 186 531 507 27 888 Cuts at: 5282 6257 1163 Size: 975 4099 Fragments arranged by size: 4099 1084 975 888 573 531 507 323 186 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4358 6643 7404 8432 1540 Size: 275 186 2357 2285 761 1028 2301 Fragments arranged by size: 2357 2301 2285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 2793 3258 3267 3793 3914 4041 4434 5339 Size: 719 465 9 526 121 127 393 905 Cuts at: 5339 5703 6105 6222 6481 7254 7339 8410 2074 Size: 364 402 117 259 773 85 1071 2857 Fragments arranged by size: 2857 1071 905 773 719 526 465 402 393 364 259 127 121 117 85 9 BsmI GAATG_Cn' Cuts at: 5229 6494 6704 6797 5229 Size: 1265 210 93 7625 Fragments arranged by size: 7625 1265 210 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3034 3331 3034 Size: 297 8896 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2115 2142 2173 2190 2424 2528 Size: 399 284 41 27 31 17 234 104 Cuts at: 2528 2582 2706 2772 2793 2887 2923 3224 3258 Size: 54 124 66 21 94 36 301 34 Cuts at: 3258 3267 3359 3377 3406 3414 3685 3793 3914 Size: 9 92 18 29 8 271 108 121 Cuts at: 3914 4041 4174 4297 4314 4434 4542 4782 4906 Size: 127 133 123 17 120 108 240 124 Cuts at: 4906 4915 4992 5066 5086 5182 5339 5536 5592 Size: 9 77 74 20 96 157 197 56 Cuts at: 5592 5703 5739 5757 5810 6105 6222 6237 6258 Size: 111 36 18 53 295 117 15 21 Cuts at: 6258 6481 6512 7254 7339 8410 1391 Size: 223 31 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 301 295 284 271 240 234 223 197 157 133 127 124 124 123 121 120 117 111 108 108 104 96 94 92 85 77 74 66 56 54 53 41 36 36 34 31 31 29 27 21 21 20 18 18 17 17 15 9 9 8 BspEI T'CCGG_A Cuts at: 4635 8856 4635 Size: 4221 4972 BspHI T'CATG_A Cuts at: 7082 8812 7082 Size: 1730 7463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3306 4729 5265 5570 6166 6285 6548 844 Size: 2462 1423 536 305 596 119 263 3489 Fragments arranged by size: 3489 2462 1423 596 536 305 263 119 BsrBI CCG'CTC Cuts at: 1394 1823 2017 2911 4419 5041 7080 1394 Size: 429 194 894 1508 622 2039 3507 Fragments arranged by size: 3507 2039 1508 894 622 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 7688 7862 65 Size: 1183 6440 174 1396 Fragments arranged by size: 6440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 2544 2946 4931 6423 7837 8024 Size: 110 635 99 402 1985 1492 1414 187 Cuts at: 8024 1700 Size: 2869 Fragments arranged by size: 2869 1985 1492 1414 635 402 187 110 99 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9193 BssSI C'ACGA_G Cuts at: 3792 7253 8265 3792 Size: 3461 1012 4720 Fragments arranged by size: 4720 3461 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3208 3271 4381 4492 794 Size: 2414 63 1110 111 5495 Fragments arranged by size: 5495 2414 1110 111 63 BstBI TT'CG_AA Cuts at: 7997 7997 Size: 9193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9193 BstXI CCAn_nnnn'nTGG Cuts at: 2629 3348 6012 6400 2629 Size: 719 2664 388 5422 Fragments arranged by size: 5422 2664 719 388 BstYI r'GATC_y Cuts at: 1972 5318 5432 6123 6523 6890 7275 7292 8744 Size: 3346 114 691 400 367 385 17 1452 Cuts at: 8744 8830 8842 9188 1972 Size: 86 12 346 1977 Fragments arranged by size: 3346 1977 1452 691 400 385 367 346 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2082 3621 2082 Size: 1539 7654 BtgI C'CryG_G Cuts at: 513 1065 2004 2721 2904 5283 513 Size: 552 939 717 183 2379 4423 Fragments arranged by size: 4423 2379 939 717 552 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2146 2941 487 Size: 683 56 920 795 6739 Fragments arranged by size: 6739 920 795 683 56 BtsI GCAGTG_nn' Cuts at: 2592 3239 3341 3359 3672 4093 4312 4379 4906 Size: 647 102 18 313 421 219 67 527 Cuts at: 4906 5322 5359 5863 6709 7503 7523 2592 Size: 416 37 504 846 794 20 4262 Fragments arranged by size: 4262 846 794 647 527 504 421 416 313 219 102 67 37 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5116 5151 529 Size: 35 4552 35 4571 Fragments arranged by size: 4571 4552 35 35 DraI TTT'AAA Cuts at: 6497 6497 Size: 9193 DraIII CAC_nnn'GTG Cuts at: 4610 4610 Size: 9193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8200 809 Size: 507 6884 1802 Fragments arranged by size: 6884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 3290 3570 Size: 54 1425 50 275 18 299 1161 280 Cuts at: 3570 3673 4592 6143 6390 6640 7529 9158 8 Size: 103 919 1551 247 250 889 1629 43 Fragments arranged by size: 1629 1551 1425 1161 919 889 299 280 275 250 247 103 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6640 1537 Size: 275 4828 4090 Fragments arranged by size: 4828 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3506 4158 4818 4877 7123 1353 Size: 2153 652 660 59 2246 3423 Fragments arranged by size: 3423 2246 2153 660 652 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4655 7780 8154 8300 387 Size: 1064 138 435 2631 3125 374 146 1280 Fragments arranged by size: 3125 2631 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3791 4039 5337 3791 Size: 248 1298 7647 Fragments arranged by size: 7647 1298 248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 3418 3659 3710 3832 4535 4799 Size: 393 762 1024 241 51 122 703 264 Cuts at: 4799 5067 5852 6127 6468 7256 7853 8640 8908 Size: 268 785 275 341 788 597 787 268 Cuts at: 8908 1239 Size: 1524 Fragments arranged by size: 1524 1024 788 787 785 762 703 597 393 341 275 268 268 264 241 122 51 EcoNI CCTnn'n_nnAGG Cuts at: 2615 4182 2615 Size: 1567 7626 EcoO109I rG'GnC_Cy Cuts at: 2112 2414 2578 3035 3221 3319 3681 3775 4170 Size: 302 164 457 186 98 362 94 395 Cuts at: 4170 4171 4187 4963 5098 5123 5362 6001 6149 Size: 1 16 776 135 25 239 639 148 Cuts at: 6149 6254 6255 2112 Size: 105 1 5050 Fragments arranged by size: 5050 776 639 457 395 362 302 239 186 164 148 135 105 98 94 25 16 1 1 EcoRI G'AATT_C Cuts at: 3944 6502 6880 3944 Size: 2558 378 6257 Fragments arranged by size: 6257 2558 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 6394 2374 Size: 4020 5173 FspI TGC'GCA Cuts at: 2942 7699 2942 Size: 4757 4436 HaeII r_GCGC'y Cuts at: 1678 2904 4203 4795 4822 5314 5416 8340 1678 Size: 1226 1299 592 27 492 102 2924 2531 Fragments arranged by size: 2924 2531 1299 1226 592 492 102 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 3099 3131 3617 Size: 32 135 32 489 32 1006 32 486 Cuts at: 3617 3649 4010 4042 4727 4759 4844 4876 5819 Size: 32 361 32 685 32 85 32 943 Cuts at: 5819 5851 6017 6049 6427 6459 7834 7866 7908 Size: 32 166 32 378 32 1375 32 42 Cuts at: 7908 7940 1373 Size: 32 2626 Fragments arranged by size: 2626 1375 1006 943 685 489 486 378 361 166 135 85 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3802 6537 6783 669 Size: 988 281 1864 2735 246 3079 Fragments arranged by size: 3079 2735 1864 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9193 HpaI GTT'AAC Cuts at: 6783 6783 Size: 9193 KpnI G_GTAC'C Cuts at: 6518 6518 Size: 9193 MfeI C'AATT_G Cuts at: 6792 6792 Size: 9193 MluI A'CGCG_T Cuts at: 9069 9069 Size: 9193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4560 5212 5953 6162 6184 8307 8491 1305 Size: 3255 652 741 209 22 2123 184 2007 Fragments arranged by size: 3255 2123 2007 741 652 209 184 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 3572 6145 6392 10 Size: 54 1425 343 1740 2573 247 2811 Fragments arranged by size: 2811 2573 1740 1425 343 247 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2630 2786 2960 3119 3884 4317 Size: 648 984 480 156 174 159 765 433 Cuts at: 4317 7151 7510 7669 518 Size: 2834 359 159 2042 Fragments arranged by size: 2834 2042 984 765 648 480 433 359 174 159 159 156 MspA1I CmG'CkG Cuts at: 1606 2135 2689 2804 2973 3205 3379 3598 3871 Size: 529 554 115 169 232 174 219 273 Cuts at: 3871 4255 4277 5194 5472 6133 6609 7286 8434 Size: 384 22 917 278 661 476 677 1148 Cuts at: 8434 8679 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 917 677 661 554 529 476 384 278 273 245 232 219 174 169 115 22 NaeI GCC'GGC Cuts at: 2546 2948 2546 Size: 402 8791 NcoI C'CATG_G Cuts at: 513 1065 2004 2721 5283 513 Size: 552 939 717 2562 4423 Fragments arranged by size: 4423 2562 939 717 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9193 NgoMIV G'CCGG_C Cuts at: 2544 2946 2544 Size: 402 8791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9193 NotI GC'GGCC_GC Cuts at: 6640 6640 Size: 9193 NsiI A_TGCA'T Cuts at: 2207 2207 Size: 9193 NspI r_CATG'y Cuts at: 1132 1229 2205 2477 2575 6551 8096 1132 Size: 97 976 272 98 3976 1545 2229 Fragments arranged by size: 3976 2229 1545 976 272 98 97 PasI CC'CwG_GG Cuts at: 1348 2191 2479 5057 1348 Size: 843 288 2578 5484 Fragments arranged by size: 5484 2578 843 288 PciI A'CATG_T Cuts at: 8092 8092 Size: 9193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 3598 6136 6266 1108 Size: 1125 1365 2538 130 4035 Fragments arranged by size: 4035 2538 1365 1125 130 PfoI T'CCnGG_A Cuts at: 4965 4965 Size: 9193 PmeI GTTT'AAAC Cuts at: 6497 6497 Size: 9193 PmlI CAC'GTG Cuts at: 6340 6340 Size: 9193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7262 7294 8775 8807 7262 Size: 32 1481 32 7648 Fragments arranged by size: 7648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4963 5123 5362 4963 Size: 160 239 8794 Fragments arranged by size: 8794 239 160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9193 PsiI TTA'TAA Cuts at: 6763 6763 Size: 9193 PspOMI G'GGCC_C Cuts at: 2111 2578 3220 3681 4170 4293 6254 2111 Size: 467 642 461 489 123 1961 5050 Fragments arranged by size: 5050 1961 642 489 467 461 123 PstI C_TGCA'G Cuts at: 830 2835 3204 3213 3822 6282 6545 830 Size: 2005 369 9 609 2460 263 3478 Fragments arranged by size: 3478 2460 2005 609 369 263 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9193 PvuII CAG'CTG Cuts at: 2135 2804 3205 3598 3871 4277 5194 5472 2135 Size: 669 401 393 273 406 917 278 5856 Fragments arranged by size: 5856 917 669 406 401 393 278 273 SacI G_AGCT'C Cuts at: 3793 4041 5339 3793 Size: 248 1298 7647 Fragments arranged by size: 7647 1298 248 SalI G'TCGA_C Cuts at: 1936 6535 1936 Size: 4599 4594 SanDI GG'GwC_CC Cuts at: 5123 5123 Size: 9193 SapI GCTCTTCn'nnn_ Cuts at: 3506 3506 Size: 9193 SbfI CC_TGCA'GG Cuts at: 3822 6545 3822 Size: 2723 6470 ScaI AGT'ACT Cuts at: 4106 7441 9153 4106 Size: 3335 1712 4146 Fragments arranged by size: 4146 3335 1712 SexAI A'CCwGG_T Cuts at: 4606 4606 Size: 9193 SfcI C'TryA_G Cuts at: 826 1045 2383 2831 3200 3209 3818 6278 6541 Size: 219 1338 448 369 9 609 2460 263 Cuts at: 6541 7676 8357 8548 826 Size: 1135 681 191 1471 Fragments arranged by size: 2460 1471 1338 1135 681 609 448 369 263 219 191 9 SfiI GGCCn_nnn'nGGCC Cuts at: 4937 4937 Size: 9193 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9193 SgrAI Cr'CCGG_yG Cuts at: 1700 6423 1700 Size: 4723 4470 SmaI CCC'GGG Cuts at: 1872 2419 3839 4976 5306 6520 1872 Size: 547 1420 1137 330 1214 4545 Fragments arranged by size: 4545 1420 1214 1137 547 330 SmlI C'TyrA_G Cuts at: 820 1017 1943 3737 4675 7297 8198 8460 820 Size: 197 926 1794 938 2622 901 262 1553 Fragments arranged by size: 2622 1794 1553 938 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6984 493 Size: 6491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9193 SphI G_CATG'C Cuts at: 1132 2477 6551 1132 Size: 1345 4074 3774 Fragments arranged by size: 4074 3774 1345 SspI AAT'ATT Cuts at: 5 52 7117 5 Size: 47 7065 2081 Fragments arranged by size: 7065 2081 47 StuI AGG'CCT Cuts at: 1517 4955 5961 1517 Size: 3438 1006 4749 Fragments arranged by size: 4749 3438 1006 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 2721 2852 3051 3703 4956 Size: 552 939 228 489 131 199 652 1253 Cuts at: 4956 5127 5283 6011 6447 6632 513 Size: 171 156 728 436 185 3074 Fragments arranged by size: 3074 1253 939 728 652 552 489 436 228 199 185 171 156 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 2246 3785 3990 5165 5724 6277 Size: 1255 509 157 1539 205 1175 559 553 Cuts at: 6277 7220 7237 7390 7575 8020 325 Size: 943 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 1175 943 559 553 509 445 205 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4104 6061 6366 Size: 274 80 33 51 1443 2127 1957 305 Cuts at: 6366 7439 9151 96 Size: 1073 1712 138 Fragments arranged by size: 2127 1957 1712 1443 1073 305 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4574 4606 4863 4895 4971 5003 9153 9185 4574 Size: 32 257 32 76 32 4150 32 4582 Fragments arranged by size: 4582 4150 257 76 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9193 XbaI T'CTAG_A Cuts at: 6529 6529 Size: 9193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2216 3611 5098 2149 Size: 67 1395 1487 6244 Fragments arranged by size: 6244 1487 1395 67 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9193 XmaI C'CCGG_G Cuts at: 1870 2417 3837 4974 5304 6518 1870 Size: 547 1420 1137 330 1214 4545 Fragments arranged by size: 4545 1420 1214 1137 547 330 XmnI GAAnn'nnTTC Cuts at: 7322 7322 Size: 9193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7001 276 Size: 53 83 186 936 5467 2468 Fragments arranged by size: 5467 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SfoI SrfI SwaI