Restriction map for
pFN21AA0476
(Linear) (Six-base) MAPSORT of: KIAA0476.seq Check: 605 from: 1 to: 4488
KIAA0476.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2724 4280 4488
Size: 2724 1556 208
Fragments arranged by size:
2724 1556 208
AccI GT'mk_AC
Cuts at: 0 1475 4488
Size: 1475 3013
AclI AA'CG_TT
Cuts at: 0 1079 4488
Size: 1079 3409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2794 4488
Size: 2794 1694
AleI CACnn'nnGTG
Cuts at: 0 145 625 781 4488
Size: 145 480 156 3707
Fragments arranged by size:
3707 480 156 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 1132 2440 3874 4488
Size: 949 183 1308 1434 614
Fragments arranged by size:
1434 1308 949 614 183
AlwNI CAG_nnn'CTG
Cuts at: 0 1203 1212 1307 1593 1869 2272 2376 4030
Size: 1203 9 95 286 276 403 104 1654
Cuts at: 4030 4147 4488
Size: 117 341
Fragments arranged by size:
1654 1203 403 341 286 276 117 104
95 9
ApaI G_GGCC'C
Cuts at: 0 110 577 1219 1680 2169 2292 4253 4488
Size: 110 467 642 461 489 123 1961 235
Fragments arranged by size:
1961 642 489 467 461 235 123 110
ApaLI G'TGCA_C
Cuts at: 0 4213 4488
Size: 4213 275
ApoI r'AATT_y
Cuts at: 0 1939 3452 4488
Size: 1939 1513 1036
Fragments arranged by size:
1939 1513 1036
AvaI C'yCGr_G
Cuts at: 0 412 688 1832 2056 2363 2900 2969 3299
Size: 412 276 1144 224 307 537 69 330
Cuts at: 3299 4488
Size: 1189
Fragments arranged by size:
1189 1144 537 412 330 307 276 224
69
BamHI G'GATC_C
Cuts at: 0 3313 3427 4488
Size: 3313 114 1061
Fragments arranged by size:
3313 1061 114
BanI G'GyrC_C
Cuts at: 0 416 817 1183 2294 2355 3151 3172 3528
Size: 416 401 366 1111 61 796 21 356
Cuts at: 3528 3646 4415 4488
Size: 118 769 73
Fragments arranged by size:
1111 796 769 416 401 366 356 118
73 61 21
BanII G_rGCy'C
Cuts at: 0 110 137 577 1219 1680 1788 2036 2169
Size: 110 27 440 642 461 108 248 133
Cuts at: 2169 2292 2777 3061 3334 3734 3805 4232 4253
Size: 123 485 284 273 400 71 427 21
Cuts at: 4253 4488
Size: 235
Fragments arranged by size:
642 485 461 440 427 400 284 273
248 235 133 123 110 108 71 27
21
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 762 1740 3280 3382 4488
Size: 696 66 978 1540 102 1106
Fragments arranged by size:
1540 1106 978 696 102 66
BbvCI CC'TCA_GC
Cuts at: 0 4411 4488
Size: 4411 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2047 2081 3695 3729 4488
Size: 2047 34 1614 34 759
Fragments arranged by size:
2047 1614 759 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1170 1432 4488
Size: 1170 262 3056
Fragments arranged by size:
3056 1170 262
BfrBI ATG'CAT
Cuts at: 0 200 4488
Size: 200 4288
BglI GCCn_nnn'nGGC
Cuts at: 0 21 2905 2932 3056 4488
Size: 21 2884 27 124 1432
Fragments arranged by size:
2884 1432 124 27 21
BlpI GC'TnA_GC
Cuts at: 0 131 892 3066 3333 4488
Size: 131 761 2174 267 1155
Fragments arranged by size:
2174 1155 761 267 131
Bme1580I G_kGCm'C
Cuts at: 0 110 168 185 419 523 577 701 767
Size: 110 58 17 234 104 54 124 66
Cuts at: 767 882 918 1219 1354 1372 1401 1409 1680
Size: 115 36 301 135 18 29 8 271
Cuts at: 1680 2169 2292 2309 2537 2901 2910 2987 3081
Size: 489 123 17 228 364 9 77 94
Cuts at: 3081 3177 3531 3587 3752 4217 4253 4488
Size: 96 354 56 165 465 36 235
Fragments arranged by size:
489 465 364 354 301 271 235 234
228 165 135 124 123 115 110 104
96 94 77 66 58 56 54 36
36 29 18 17 17 9 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 178 500 892 928 1396 2091 3075
Size: 95 83 322 392 36 468 695 984
Cuts at: 3075 3614 3935 4488
Size: 539 321 553
Fragments arranged by size:
984 695 553 539 468 392 322 321
95 83 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1726 1923 4488
Size: 1726 197 2565
Fragments arranged by size:
2565 1726 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1413 1654 1705 1827 2530 3062 3847
Size: 389 1024 241 51 122 703 532 785
Cuts at: 3847 4122 4463 4488
Size: 275 341 25
Fragments arranged by size:
1024 785 703 532 389 341 275 241
122 51 25
Bpu10I CC'TnA_GC
Cuts at: 0 1220 1988 2279 3013 3060 3236 3799 4411
Size: 1220 768 291 734 47 176 563 612
Cuts at: 4411 4488
Size: 77
Fragments arranged by size:
1220 768 734 612 563 291 176 77
47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1717 2691 4488
Size: 1717 974 1797
Fragments arranged by size:
1797 1717 974
BsaI GGTCTCn'nnnn_
Cuts at: 0 1095 2840 4488
Size: 1095 1745 1648
Fragments arranged by size:
1745 1648 1095
BsaAI yAC'GTr
Cuts at: 0 4335 4488
Size: 4335 153
BsaHI Gr'CG_yC
Cuts at: 0 2610 2868 4488
Size: 2610 258 1620
Fragments arranged by size:
2610 1620 258
BsaWI w'CCGG_w
Cuts at: 0 2630 4488
Size: 2630 1858
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1003 1033 2722 2752 3814 3844 4488
Size: 1003 30 1689 30 1062 30 644
Fragments arranged by size:
1689 1062 1003 644 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 515 676 1135 1510 1633 1915 2029
Size: 22 493 161 459 375 123 282 114
Cuts at: 2029 2074 2204 3043 4420 4488
Size: 45 130 839 1377 68
Fragments arranged by size:
1377 839 493 459 375 282 161 130
123 114 68 45 22
BseYI C'CCAG_C
Cuts at: 0 46 214 312 576 645 2200 2301 2618
Size: 46 168 98 264 69 1555 101 317
Cuts at: 2618 2830 2850 3168 3261 3524 3628 3734 4124
Size: 212 20 318 93 263 104 106 390
Cuts at: 4124 4488
Size: 364
Fragments arranged by size:
1555 390 364 318 317 264 263 212
168 106 104 101 98 93 69 46
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1138 1324 1855 2362 2389 3277 4252
Size: 815 323 186 531 507 27 888 975
Cuts at: 4252 4488
Size: 236
Fragments arranged by size:
975 888 815 531 507 323 236 186
27
BsiEI CG_ry'CG
Cuts at: 0 2353 4488
Size: 2353 2135
BsiHKAI G_wGCw'C
Cuts at: 0 69 788 1253 1262 1788 1909 2036 2429
Size: 69 719 465 9 526 121 127 393
Cuts at: 2429 3334 3698 4100 4217 4476 4488
Size: 905 364 402 117 259 12
Fragments arranged by size:
905 719 526 465 402 393 364 259
127 121 117 69 12 9
BsmI GAATG_Cn'
Cuts at: 0 3224 4488
Size: 3224 1264
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1029 1326 4488
Size: 1029 297 3162
Fragments arranged by size:
3162 1029 297
Bsp1286I G_dGCh'C
Cuts at: 0 69 110 137 168 185 419 523 577
Size: 69 41 27 31 17 234 104 54
Cuts at: 577 701 767 788 882 918 1219 1253 1262
Size: 124 66 21 94 36 301 34 9
Cuts at: 1262 1354 1372 1401 1409 1680 1788 1909 2036
Size: 92 18 29 8 271 108 121 127
Cuts at: 2036 2169 2292 2309 2429 2537 2777 2901 2910
Size: 133 123 17 120 108 240 124 9
Cuts at: 2910 2987 3061 3081 3177 3334 3531 3587 3698
Size: 77 74 20 96 157 197 56 111
Cuts at: 3698 3734 3752 3805 4100 4217 4232 4253 4476
Size: 36 18 53 295 117 15 21 223
Cuts at: 4476 4488
Size: 12
Fragments arranged by size:
301 295 271 240 234 223 197 157
133 127 124 124 123 121 120 117
111 108 108 104 96 94 92 77
74 69 66 56 54 53 41 36
36 34 31 29 27 21 21 20
18 18 17 17 15 12 9 9
8
BspEI T'CCGG_A
Cuts at: 0 2630 4488
Size: 2630 1858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1301 2724 3260 3565 4161 4280 4488
Size: 1301 1423 536 305 596 119 208
Fragments arranged by size:
1423 1301 596 536 305 208 119
BsrBI CCG'CTC
Cuts at: 0 12 906 2414 3036 4488
Size: 12 894 1508 622 1452
Fragments arranged by size:
1508 1452 894 622 12
BsrFI r'CCGG_y
Cuts at: 0 440 539 941 2926 4418 4488
Size: 440 99 402 1985 1492 70
Fragments arranged by size:
1985 1492 440 402 99 70
BssSI C'ACGA_G
Cuts at: 0 1787 4488
Size: 1787 2701
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1203 1266 2376 2487 4488
Size: 1203 63 1110 111 2001
Fragments arranged by size:
2001 1203 1110 111 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 1343 4007 4395 4488
Size: 624 719 2664 388 93
Fragments arranged by size:
2664 719 624 388 93
BstYI r'GATC_y
Cuts at: 0 3313 3427 4118 4488
Size: 3313 114 691 370
Fragments arranged by size:
3313 691 370 114
Bsu36I CC'TnA_GG
Cuts at: 0 77 1616 4488
Size: 77 1539 2872
Fragments arranged by size:
2872 1539 77
BtgI C'CryG_G
Cuts at: 0 716 899 3278 4488
Size: 716 183 2379 1210
Fragments arranged by size:
2379 1210 716 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 141 936 4488
Size: 141 795 3552
Fragments arranged by size:
3552 795 141
BtsI GCAGTG_nn'
Cuts at: 0 587 1234 1336 1354 1667 2088 2307 2374
Size: 587 647 102 18 313 421 219 67
Cuts at: 2374 2901 3317 3354 3858 4488
Size: 527 416 37 504 630
Fragments arranged by size:
647 630 587 527 504 421 416 313
219 102 67 37 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3111 3146 4488
Size: 3111 35 1342
Fragments arranged by size:
3111 1342 35
DraIII CAC_nnn'GTG
Cuts at: 0 2605 4488
Size: 2605 1883
EaeI y'GGCC_r
Cuts at: 0 124 1285 1565 1668 2587 4138 4385 4488
Size: 124 1161 280 103 919 1551 247 103
Fragments arranged by size:
1551 1161 919 280 247 124 103 103
EarI CTCTTCn'nnn_
Cuts at: 0 1501 2153 2813 2872 4488
Size: 1501 652 660 59 1616
Fragments arranged by size:
1616 1501 660 652 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2650 4488
Size: 19 2631 1838
Fragments arranged by size:
2631 1838 19
EcoICRI GAG'CTC
Cuts at: 0 1786 2034 3332 4488
Size: 1786 248 1298 1156
Fragments arranged by size:
1786 1298 1156 248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1413 1654 1705 1827 2530 2794 3062
Size: 389 1024 241 51 122 703 264 268
Cuts at: 3062 3847 4122 4463 4488
Size: 785 275 341 25
Fragments arranged by size:
1024 785 703 389 341 275 268 264
241 122 51 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 2177 4488
Size: 610 1567 2311
Fragments arranged by size:
2311 1567 610
EcoO109I rG'GnC_Cy
Cuts at: 0 107 409 573 1030 1216 1314 1676 1770
Size: 107 302 164 457 186 98 362 94
Cuts at: 1770 2165 2166 2182 2958 3093 3118 3357 3996
Size: 395 1 16 776 135 25 239 639
Cuts at: 3996 4144 4249 4250 4488
Size: 148 105 1 238
Fragments arranged by size:
776 639 457 395 362 302 239 238
186 164 148 135 107 105 98 94
25 16 1 1
EcoRI G'AATT_C
Cuts at: 0 1939 4488
Size: 1939 2549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 4389 4488
Size: 369 4020 99
Fragments arranged by size:
4020 369 99
FspI TGC'GCA
Cuts at: 0 937 4488
Size: 937 3551
HaeII r_GCGC'y
Cuts at: 0 899 2198 2790 2817 3309 3411 4488
Size: 899 1299 592 27 492 102 1077
Fragments arranged by size:
1299 1077 899 592 492 102 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 1094 1126 1612 1644 2005 2037
Size: 56 32 1006 32 486 32 361 32
Cuts at: 2037 2722 2754 2839 2871 3814 3846 4012 4044
Size: 685 32 85 32 943 32 166 32
Cuts at: 4044 4422 4454 4488
Size: 378 32 34
Fragments arranged by size:
1006 943 685 486 378 361 166 85
56 34 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1797 4488
Size: 1797 2691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2555 3207 3948 4157 4179 4488
Size: 2555 652 741 209 22 309
Fragments arranged by size:
2555 741 652 309 209 22
MscI TGG'CCA
Cuts at: 0 1567 4140 4387 4488
Size: 1567 2573 247 101
Fragments arranged by size:
2573 1567 247 101
MslI CAynn'nnrTG
Cuts at: 0 145 625 781 955 1114 1879 2312 4488
Size: 145 480 156 174 159 765 433 2176
Fragments arranged by size:
2176 765 480 433 174 159 156 145
MspA1I CmG'CkG
Cuts at: 0 130 684 799 968 1200 1374 1593 1866
Size: 130 554 115 169 232 174 219 273
Cuts at: 1866 2250 2272 3189 3467 4128 4488
Size: 384 22 917 278 661 360
Fragments arranged by size:
917 661 554 384 360 278 273 232
219 174 169 130 115 22
NaeI GCC'GGC
Cuts at: 0 541 943 4488
Size: 541 402 3545
Fragments arranged by size:
3545 541 402
NcoI C'CATG_G
Cuts at: 0 716 3278 4488
Size: 716 2562 1210
Fragments arranged by size:
2562 1210 716
NgoMIV G'CCGG_C
Cuts at: 0 539 941 4488
Size: 539 402 3547
Fragments arranged by size:
3547 539 402
NsiI A_TGCA'T
Cuts at: 0 202 4488
Size: 202 4286
NspI r_CATG'y
Cuts at: 0 200 472 570 4488
Size: 200 272 98 3918
Fragments arranged by size:
3918 272 200 98
PasI CC'CwG_GG
Cuts at: 0 186 474 3052 4488
Size: 186 288 2578 1436
Fragments arranged by size:
2578 1436 288 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 1593 4131 4261 4488
Size: 228 1365 2538 130 227
Fragments arranged by size:
2538 1365 228 227 130
PfoI T'CCnGG_A
Cuts at: 0 2960 4488
Size: 2960 1528
PmlI CAC'GTG
Cuts at: 0 4335 4488
Size: 4335 153
PpuMI rG'GwC_Cy
Cuts at: 0 2958 3118 3357 4488
Size: 2958 160 239 1131
Fragments arranged by size:
2958 1131 239 160
PspOMI G'GGCC_C
Cuts at: 0 106 573 1215 1676 2165 2288 4249 4488
Size: 106 467 642 461 489 123 1961 239
Fragments arranged by size:
1961 642 489 467 461 239 123 106
PstI C_TGCA'G
Cuts at: 0 830 1199 1208 1817 4277 4488
Size: 830 369 9 609 2460 211
Fragments arranged by size:
2460 830 609 369 211 9
PvuII CAG'CTG
Cuts at: 0 130 799 1200 1593 1866 2272 3189 3467
Size: 130 669 401 393 273 406 917 278
Cuts at: 3467 4488
Size: 1021
Fragments arranged by size:
1021 917 669 406 401 393 278 273
130
SacI G_AGCT'C
Cuts at: 0 1788 2036 3334 4488
Size: 1788 248 1298 1154
Fragments arranged by size:
1788 1298 1154 248
SanDI GG'GwC_CC
Cuts at: 0 3118 4488
Size: 3118 1370
SapI GCTCTTCn'nnn_
Cuts at: 0 1501 4488
Size: 1501 2987
SbfI CC_TGCA'GG
Cuts at: 0 1817 4488
Size: 1817 2671
ScaI AGT'ACT
Cuts at: 0 2101 4488
Size: 2101 2387
SexAI A'CCwGG_T
Cuts at: 0 2601 4488
Size: 2601 1887
SfcI C'TryA_G
Cuts at: 0 378 826 1195 1204 1813 4273 4488
Size: 378 448 369 9 609 2460 215
Fragments arranged by size:
2460 609 448 378 369 215 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2932 4488
Size: 2932 1556
SgrAI Cr'CCGG_yG
Cuts at: 0 4418 4488
Size: 4418 70
SmaI CCC'GGG
Cuts at: 0 414 1834 2971 3301 4488
Size: 414 1420 1137 330 1187
Fragments arranged by size:
1420 1187 1137 414 330
SmlI C'TyrA_G
Cuts at: 0 1732 2670 4488
Size: 1732 938 1818
Fragments arranged by size:
1818 1732 938
SphI G_CATG'C
Cuts at: 0 472 4488
Size: 472 4016
StuI AGG'CCT
Cuts at: 0 2950 3956 4488
Size: 2950 1006 532
Fragments arranged by size:
2950 1006 532
StyI C'CwwG_G
Cuts at: 0 227 716 847 1046 1698 2951 3122 3278
Size: 227 489 131 199 652 1253 171 156
Cuts at: 3278 4006 4442 4488
Size: 728 436 46
Fragments arranged by size:
1253 728 652 489 436 227 199 171
156 131 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 241 1780 1985 3160 3719 4272 4488
Size: 84 157 1539 205 1175 559 553 216
Fragments arranged by size:
1539 1175 559 553 216 205 157 84
TatI w'GTAC_w
Cuts at: 0 2099 4056 4361 4488
Size: 2099 1957 305 127
Fragments arranged by size:
2099 1957 305 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2569 2601 2858 2890 2966 2998 4488
Size: 2569 32 257 32 76 32 1490
Fragments arranged by size:
2569 1490 257 76 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 211 1606 3093 4488
Size: 144 67 1395 1487 1395
Fragments arranged by size:
1487 1395 1395 144 67
XmaI C'CCGG_G
Cuts at: 0 412 1832 2969 3299 4488
Size: 412 1420 1137 330 1189
Fragments arranged by size:
1420 1189 1137 412 330
Enzymes that do cut:
AarI AccI AclI AcuI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BbeI BclI BglII
BmgBI BmtI BsaBI BsiWI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I ClaI DraI DrdI EagI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SfoI SgfI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI