(Circular) (Six-base) MAPSORT of: pFN21AA0474.seq Check: 803 from: 1 to: 6748 pFN21AA0474 6748 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 2391 Size: 6748 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2496 4558 278 Size: 53 83 186 936 960 2062 2468 Fragments arranged by size: 2468 2062 960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4091 1937 Size: 2154 4594 Acc65I G'GTAC_C Cuts at: 4069 4069 Size: 6748 AclI AA'CG_TT Cuts at: 1563 4875 5248 1563 Size: 3312 373 3063 Fragments arranged by size: 3312 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2620 4811 6195 6463 1632 Size: 988 2191 1384 268 1917 Fragments arranged by size: 2191 1917 1384 988 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6551 AflIII A'CryG_T Cuts at: 1203 2805 5647 6624 1203 Size: 1602 2842 977 1327 Fragments arranged by size: 2842 1602 1327 977 AgeI A'CCGG_T Cuts at: 5579 5579 Size: 6748 AhdI GACnn_n'nnGTC Cuts at: 5477 5477 Size: 6748 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6748 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3195 3227 3195 Size: 32 6716 AlwNI CAG_nnn'CTG Cuts at: 4491 6063 4491 Size: 1572 5176 ApaI G_GGCC'C Cuts at: 2984 3557 2984 Size: 573 6175 ApaLI G'TGCA_C Cuts at: 2102 4805 5961 2102 Size: 2703 1156 2889 Fragments arranged by size: 2889 2703 1156 ApoI r'AATT_y Cuts at: 1493 2678 3092 4057 4283 4435 6544 1493 Size: 1185 414 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 1185 965 414 226 152 AseI AT'TA_AT Cuts at: 160 1032 5302 160 Size: 872 4270 1606 Fragments arranged by size: 4270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2204 2246 3992 4073 1870 Size: 73 152 109 42 1746 81 4545 Fragments arranged by size: 4545 1746 152 109 81 73 42 BamHI G'GATC_C Cuts at: 4078 4445 4078 Size: 367 6381 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 4069 5524 618 Size: 325 217 627 390 1892 1455 1842 Fragments arranged by size: 1892 1842 1455 627 390 325 217 BanII G_rGCy'C Cuts at: 1391 2984 3536 3557 3629 3678 3707 4067 1391 Size: 1593 552 21 72 49 29 360 4072 Fragments arranged by size: 4072 1593 552 360 72 49 29 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2335 2496 2884 3650 928 Size: 971 436 161 388 766 4026 Fragments arranged by size: 4026 971 766 436 388 161 BbvCI CC'TCA_GC Cuts at: 2970 2970 Size: 6748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4939 4973 1903 Size: 34 3002 34 3678 Fragments arranged by size: 3678 3002 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4640 5850 4640 Size: 1210 5538 BclI T'GATC_A Cuts at: 1548 3111 1548 Size: 1563 5185 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2988 3422 3561 Size: 107 122 71 631 605 1316 434 139 Cuts at: 3561 5359 136 Size: 1798 1525 Fragments arranged by size: 1798 1525 1316 631 605 434 139 122 107 71 BglII A'GATC_T Cuts at: 6743 6743 Size: 6748 BlpI GC'TnA_GC Cuts at: 3344 4165 3344 Size: 821 5927 Bme1580I G_kGCm'C Cuts at: 1790 2106 2180 2922 2984 3033 3557 3663 4809 Size: 316 74 742 62 49 524 106 1146 Cuts at: 4809 5965 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1146 742 524 316 106 74 62 49 BmgBI CAC'GTC Cuts at: 1318 2737 3664 1318 Size: 1419 927 4402 Fragments arranged by size: 4402 1419 927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 4479 5437 Size: 416 53 83 399 24 729 2333 958 Cuts at: 5437 442 Size: 1753 Fragments arranged by size: 2333 1753 958 729 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3179 3206 3878 3941 5408 1239 Size: 1940 27 672 63 1467 2579 Fragments arranged by size: 2579 1940 1467 672 63 27 Bpu10I CC'TnA_GC Cuts at: 2396 2970 2396 Size: 574 6174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 3752 4873 5738 6036 2336 Size: 1416 1121 865 298 3048 Fragments arranged by size: 3048 1416 1121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3010 3734 5411 882 Size: 617 1511 724 1677 2219 Fragments arranged by size: 2219 1677 1511 724 617 BsaAI yAC'GTr Cuts at: 493 1204 2806 3915 4539 493 Size: 711 1602 1109 624 2702 Fragments arranged by size: 2702 1602 1109 711 624 BsaBI GATnn'nnATC Cuts at: 4001 6742 4001 Size: 2741 4007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 2592 2838 3472 Size: 53 83 186 936 960 99 246 634 Cuts at: 3472 3963 4555 4937 5572 275 Size: 491 592 382 635 1451 Fragments arranged by size: 1451 960 936 635 634 592 491 382 246 186 99 83 53 BsaWI w'CCGG_w Cuts at: 2274 3309 5181 5579 5853 6000 6411 2274 Size: 1035 1872 398 274 147 411 2611 Fragments arranged by size: 2611 1872 1035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2783 2813 3033 3063 3665 Size: 30 165 30 1380 30 220 30 602 Cuts at: 3665 3695 3851 3881 1178 Size: 30 156 30 4045 Fragments arranged by size: 4045 1380 602 220 165 156 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2739 3176 3775 3836 1186 Size: 904 649 437 599 61 4098 Fragments arranged by size: 4098 904 649 599 437 61 BseYI C'CCAG_C Cuts at: 2841 4035 4039 5951 6447 2841 Size: 1194 4 1912 496 3142 Fragments arranged by size: 3142 1912 1194 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2984 3629 1163 Size: 573 1248 645 4282 Fragments arranged by size: 4282 1248 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4198 4959 5987 1540 Size: 275 186 2197 761 1028 2301 Fragments arranged by size: 2301 2197 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2382 2523 3707 4809 4894 5965 2106 Size: 276 141 1184 1102 85 1071 2889 Fragments arranged by size: 2889 1184 1102 1071 276 141 85 BsmI GAATG_Cn' Cuts at: 4259 4352 4259 Size: 93 6655 BsmBI CGTCTCn'nnnn_ Cuts at: 3182 3670 3818 3182 Size: 488 148 6112 Fragments arranged by size: 6112 488 148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2180 2382 2523 2922 2984 3033 Size: 399 316 74 202 141 399 62 49 Cuts at: 3033 3536 3557 3629 3663 3678 3707 4067 4809 Size: 503 21 72 34 15 29 360 742 Cuts at: 4809 4894 5965 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 503 399 399 360 316 202 141 85 74 72 62 49 34 29 21 15 BspEI T'CCGG_A Cuts at: 3309 6411 3309 Size: 3102 3646 BspHI T'CATG_A Cuts at: 4637 6367 4637 Size: 1730 5018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 4103 844 Size: 1547 1712 3489 Fragments arranged by size: 3489 1712 1547 BsrBI CCG'CTC Cuts at: 1394 1823 3166 3737 3783 4635 1394 Size: 429 1343 571 46 852 3507 Fragments arranged by size: 3507 1343 852 571 429 46 BsrDI GCAATG_nn' Cuts at: 65 1248 3434 5243 5417 65 Size: 1183 2186 1809 174 1396 Fragments arranged by size: 2186 1809 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3965 5392 5579 1700 Size: 110 2155 1427 187 2869 Fragments arranged by size: 2869 2155 1427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6748 BssSI C'ACGA_G Cuts at: 2105 3538 4808 5820 2105 Size: 1433 1270 1012 3033 Fragments arranged by size: 3033 1433 1270 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4030 794 Size: 3236 3512 BstBI TT'CG_AA Cuts at: 5552 5552 Size: 6748 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6748 BstXI CCAn_nnnn'nTGG Cuts at: 3817 3817 Size: 6748 BstYI r'GATC_y Cuts at: 1972 4002 4078 4445 4830 4847 6299 6385 6397 Size: 2030 76 367 385 17 1452 86 12 Cuts at: 6397 6743 1972 Size: 346 1977 Fragments arranged by size: 2030 1977 1452 385 367 346 86 76 17 12 Bsu36I CC'TnA_GG Cuts at: 3874 3874 Size: 6748 BtgI C'CryG_G Cuts at: 513 1065 2743 3327 3336 3501 513 Size: 552 1678 584 9 165 3760 Fragments arranged by size: 3760 1678 584 552 165 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6009 Fragments arranged by size: 6009 683 56 BtsI GCAGTG_nn' Cuts at: 2216 3012 3274 3487 4264 5058 5078 2216 Size: 796 262 213 777 794 20 3886 Fragments arranged by size: 3886 796 794 777 262 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6748 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6713 DraI TTT'AAA Cuts at: 4052 4052 Size: 6748 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2564 5755 809 Size: 507 1248 3191 1802 Fragments arranged by size: 3191 1802 1248 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2332 2686 3414 Size: 54 1425 50 275 18 502 354 728 Cuts at: 3414 4195 5084 6713 8 Size: 781 889 1629 43 Fragments arranged by size: 1629 1425 889 781 728 502 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4195 1537 Size: 275 2383 4090 Fragments arranged by size: 4090 2383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2639 3501 3524 3611 4678 1353 Size: 1286 862 23 87 1067 3423 Fragments arranged by size: 3423 1286 1067 862 87 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5335 5709 5855 387 Size: 1064 138 3746 374 146 1280 Fragments arranged by size: 3746 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3705 3705 Size: 6748 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2620 3179 3206 3878 3941 4811 5408 Size: 393 988 559 27 672 63 870 597 Cuts at: 5408 6195 6463 1239 Size: 787 268 1524 Fragments arranged by size: 1524 988 870 787 672 597 559 393 268 63 27 EcoNI CCTnn'n_nnAGG Cuts at: 2999 3416 2999 Size: 417 6331 EcoO109I rG'GnC_Cy Cuts at: 2126 2980 2981 3077 3979 2126 Size: 854 1 96 902 4895 Fragments arranged by size: 4895 902 854 96 1 EcoRI G'AATT_C Cuts at: 4057 4435 4057 Size: 378 6370 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2676 2676 Size: 6748 FspI TGC'GCA Cuts at: 5254 5254 Size: 6748 HaeII r_GCGC'y Cuts at: 1678 3582 3767 5895 1678 Size: 1904 185 2128 2531 Fragments arranged by size: 2531 2128 1904 185 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3033 3065 3665 3677 3697 Size: 32 135 32 1461 32 600 12 20 Cuts at: 3697 3709 3851 3883 5389 5421 5463 5495 1373 Size: 12 142 32 1506 32 42 32 2626 Fragments arranged by size: 2626 1506 1461 600 142 135 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4092 4338 669 Size: 988 281 2154 246 3079 Fragments arranged by size: 3079 2154 988 281 246 HindIII A'AGCT_T Cuts at: 748 2606 748 Size: 1858 4890 HpaI GTT'AAC Cuts at: 4338 4338 Size: 6748 KpnI G_GTAC'C Cuts at: 4073 4073 Size: 6748 MfeI C'AATT_G Cuts at: 4347 4347 Size: 6748 MluI A'CGCG_T Cuts at: 6624 6624 Size: 6748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2961 3663 5862 6046 1305 Size: 1656 702 2199 184 2007 Fragments arranged by size: 2199 2007 1656 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2688 3416 10 Size: 54 1425 343 856 728 3342 Fragments arranged by size: 3342 1425 856 728 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4706 5065 5224 518 Size: 648 3540 359 159 2042 Fragments arranged by size: 3540 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2854 3062 3384 3657 4009 4039 4164 4841 Size: 1248 208 322 273 352 30 125 677 Cuts at: 4841 5989 6234 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1248 1148 677 352 322 273 245 208 125 30 NaeI GCC'GGC Cuts at: 3967 3967 Size: 6748 NcoI C'CATG_G Cuts at: 513 1065 3327 3336 513 Size: 552 2262 9 3925 Fragments arranged by size: 3925 2262 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6748 NgoMIV G'CCGG_C Cuts at: 3965 3965 Size: 6748 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6748 NotI GC'GGCC_GC Cuts at: 4195 4195 Size: 6748 NspI r_CATG'y Cuts at: 1132 1229 2567 2951 4033 4106 5651 1132 Size: 97 1338 384 1082 73 1545 2229 Fragments arranged by size: 2229 1545 1338 1082 384 97 73 PasI CC'CwG_GG Cuts at: 1348 3524 1348 Size: 2176 4572 PciI A'CATG_T Cuts at: 5647 5647 Size: 6748 PflMI CCAn_nnn'nTGG Cuts at: 1108 3328 1108 Size: 2220 4528 PfoI T'CCnGG_A Cuts at: 2895 3051 3087 3183 2895 Size: 156 36 96 6460 Fragments arranged by size: 6460 156 96 36 PmeI GTTT'AAAC Cuts at: 4052 4052 Size: 6748 PmlI CAC'GTG Cuts at: 2806 3915 2806 Size: 1109 5639 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4817 4849 6330 6362 4817 Size: 32 1481 32 5203 Fragments arranged by size: 5203 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2126 3979 2126 Size: 1853 4895 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6748 PsiI TTA'TAA Cuts at: 4318 4318 Size: 6748 PspOMI G'GGCC_C Cuts at: 2980 3553 2980 Size: 573 6175 PspXI vC'TCGA_Gb Cuts at: 2246 2246 Size: 6748 PstI C_TGCA'G Cuts at: 830 2223 2706 4100 830 Size: 1393 483 1394 3478 Fragments arranged by size: 3478 1394 1393 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6748 PvuII CAG'CTG Cuts at: 4009 4039 4009 Size: 30 6718 SacI G_AGCT'C Cuts at: 3707 3707 Size: 6748 SalI G'TCGA_C Cuts at: 1936 4090 1936 Size: 2154 4594 SanDI GG'GwC_CC Cuts at: 3979 3979 Size: 6748 SapI GCTCTTCn'nnn_ Cuts at: 3524 3524 Size: 6748 SbfI CC_TGCA'GG Cuts at: 4100 4100 Size: 6748 ScaI AGT'ACT Cuts at: 4996 6708 4996 Size: 1712 5036 SfcI C'TryA_G Cuts at: 826 1045 2219 2702 3907 4096 5231 5912 6103 Size: 219 1174 483 1205 189 1135 681 191 Cuts at: 6103 826 Size: 1471 Fragments arranged by size: 1471 1205 1174 1135 681 483 219 191 189 SfiI GGCCn_nnn'nGGCC Cuts at: 2988 2988 Size: 6748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6748 SmaI CCC'GGG Cuts at: 1872 4075 1872 Size: 2203 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 2351 3767 4852 5753 6015 Size: 197 926 303 105 1416 1085 901 262 Cuts at: 6015 820 Size: 1553 Fragments arranged by size: 1553 1416 1085 926 901 303 262 197 105 SnaBI TAC'GTA Cuts at: 493 4539 493 Size: 4046 2702 SpeI A'CTAG_T Cuts at: 152 3941 152 Size: 3789 2959 SphI G_CATG'C Cuts at: 1132 2951 4106 1132 Size: 1819 1155 3774 Fragments arranged by size: 3774 1819 1155 SspI AAT'ATT Cuts at: 5 52 4672 5 Size: 47 4620 2081 Fragments arranged by size: 4620 2081 47 StuI AGG'CCT Cuts at: 1517 2730 3855 1517 Size: 1213 1125 4410 Fragments arranged by size: 4410 1213 1125 StyI C'CwwG_G Cuts at: 513 1065 2235 2529 3327 3336 3417 4187 513 Size: 552 1170 294 798 9 81 770 3074 Fragments arranged by size: 3074 1170 798 770 552 294 81 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2774 4775 4792 4945 5130 5575 325 Size: 1255 1194 2001 17 153 185 445 1498 Fragments arranged by size: 2001 1498 1255 1194 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 4994 6706 Size: 274 80 33 51 1443 792 2225 1712 Cuts at: 6706 96 Size: 138 Fragments arranged by size: 2225 1712 1443 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3206 3238 3504 3536 3971 4003 6708 6740 3206 Size: 32 266 32 435 32 2705 32 3214 Fragments arranged by size: 3214 2705 435 266 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3929 1316 Size: 2613 4135 XbaI T'CTAG_A Cuts at: 4084 4084 Size: 6748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3232 3334 3232 Size: 102 6646 XhoI C'TCGA_G Cuts at: 1943 2246 1943 Size: 303 6445 XmaI C'CCGG_G Cuts at: 1870 4073 1870 Size: 2203 4545 XmnI GAAnn'nnTTC Cuts at: 4877 4877 Size: 6748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2494 4556 276 Size: 53 83 186 936 960 2062 2468 Fragments arranged by size: 2468 2062 960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SexAI SfoI SrfI SwaI