Restriction map for
pFN21AA0474
(Linear) (Six-base) MAPSORT of: KIAA0474.seq Check: 5471 from: 1 to: 2043
KIAA0474.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 2043
Size: 386 1657
AatII G_ACGT'C
Cuts at: 0 491 2043
Size: 491 1552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 2043
Size: 615 1428
AflIII A'CryG_T
Cuts at: 0 800 2043
Size: 800 1243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1190 1222 2043
Size: 1190 32 821
Fragments arranged by size:
1190 821 32
ApaI G_GGCC'C
Cuts at: 0 979 1552 2043
Size: 979 573 491
Fragments arranged by size:
979 573 491
ApaLI G'TGCA_C
Cuts at: 0 97 2043
Size: 97 1946
ApoI r'AATT_y
Cuts at: 0 673 1087 2043
Size: 673 414 956
Fragments arranged by size:
956 673 414
AvaI C'yCGr_G
Cuts at: 0 90 199 241 1987 2043
Size: 90 109 42 1746 56
Fragments arranged by size:
1746 109 90 56 42
BanI G'GyrC_C
Cuts at: 0 172 2043
Size: 172 1871
BanII G_rGCy'C
Cuts at: 0 979 1531 1552 1624 1673 1702 2043
Size: 979 552 21 72 49 29 341
Fragments arranged by size:
979 552 341 72 49 29 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 491 879 1645 2043
Size: 330 161 388 766 398
Fragments arranged by size:
766 398 388 330 161
BbvCI CC'TCA_GC
Cuts at: 0 965 2043
Size: 965 1078
BclI T'GATC_A
Cuts at: 0 1106 2043
Size: 1106 937
BglI GCCn_nnn'nGGC
Cuts at: 0 983 1417 1556 2043
Size: 983 434 139 487
Fragments arranged by size:
983 487 434 139
BlpI GC'TnA_GC
Cuts at: 0 1339 2043
Size: 1339 704
Bme1580I G_kGCm'C
Cuts at: 0 101 175 917 979 1028 1552 1658 2043
Size: 101 74 742 62 49 524 106 385
Fragments arranged by size:
742 524 385 106 101 74 62 49
BmgBI CAC'GTC
Cuts at: 0 732 1659 2043
Size: 732 927 384
Fragments arranged by size:
927 732 384
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 2043
Size: 141 1902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1201 1873 1936 2043
Size: 1174 27 672 63 107
Fragments arranged by size:
1174 672 107 63 27
Bpu10I CC'TnA_GC
Cuts at: 0 391 965 2043
Size: 391 574 1078
Fragments arranged by size:
1078 574 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1747 2043
Size: 331 1416 296
Fragments arranged by size:
1416 331 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 1005 1729 2043
Size: 1005 724 314
Fragments arranged by size:
1005 724 314
BsaAI yAC'GTr
Cuts at: 0 801 1910 2043
Size: 801 1109 133
Fragments arranged by size:
1109 801 133
BsaBI GATnn'nnATC
Cuts at: 0 1996 2043
Size: 1996 47
BsaHI Gr'CG_yC
Cuts at: 0 488 587 833 1467 1958 2043
Size: 488 99 246 634 491 85
Fragments arranged by size:
634 491 488 246 99 85
BsaWI w'CCGG_w
Cuts at: 0 269 1304 2043
Size: 269 1035 739
Fragments arranged by size:
1035 739 269
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 778 808 1028 1058 1660 1690 1846 1876
Size: 778 30 220 30 602 30 156 30
Cuts at: 1876 2043
Size: 167
Fragments arranged by size:
778 602 220 167 156 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 734 1171 1770 1831 2043
Size: 85 649 437 599 61 212
Fragments arranged by size:
649 599 437 212 85 61
BseYI C'CCAG_C
Cuts at: 0 836 2030 2034 2043
Size: 836 1194 4 9
Fragments arranged by size:
1194 836 9 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1624 2043
Size: 979 645 419
Fragments arranged by size:
979 645 419
BsiHKAI G_wGCw'C
Cuts at: 0 101 377 518 1702 2043
Size: 101 276 141 1184 341
Fragments arranged by size:
1184 341 276 141 101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1177 1665 1813 2043
Size: 1177 488 148 230
Fragments arranged by size:
1177 488 230 148
Bsp1286I G_dGCh'C
Cuts at: 0 101 175 377 518 917 979 1028 1531
Size: 101 74 202 141 399 62 49 503
Cuts at: 1531 1552 1624 1658 1673 1702 2043
Size: 21 72 34 15 29 341
Fragments arranged by size:
503 399 341 202 141 101 74 72
62 49 34 29 21 15
BspEI T'CCGG_A
Cuts at: 0 1304 2043
Size: 1304 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 2043
Size: 386 1657
BsrBI CCG'CTC
Cuts at: 0 1161 1732 1778 2043
Size: 1161 571 46 265
Fragments arranged by size:
1161 571 265 46
BsrDI GCAATG_nn'
Cuts at: 0 1429 2043
Size: 1429 614
BsrFI r'CCGG_y
Cuts at: 0 1960 2043
Size: 1960 83
BssSI C'ACGA_G
Cuts at: 0 100 1533 2043
Size: 100 1433 510
Fragments arranged by size:
1433 510 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2025 2043
Size: 2025 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1812 2043
Size: 1812 231
BstYI r'GATC_y
Cuts at: 0 1997 2043
Size: 1997 46
Bsu36I CC'TnA_GG
Cuts at: 0 1869 2043
Size: 1869 174
BtgI C'CryG_G
Cuts at: 0 738 1322 1331 1496 2043
Size: 738 584 9 165 547
Fragments arranged by size:
738 584 547 165 9
BtsI GCAGTG_nn'
Cuts at: 0 211 1007 1269 1482 2043
Size: 211 796 262 213 561
Fragments arranged by size:
796 561 262 213 211
DrdI GACnn_nn'nnGTC
Cuts at: 0 559 2043
Size: 559 1484
EaeI y'GGCC_r
Cuts at: 0 327 681 1409 2043
Size: 327 354 728 634
Fragments arranged by size:
728 634 354 327
EarI CTCTTCn'nnn_
Cuts at: 0 634 1496 1519 1606 2043
Size: 634 862 23 87 437
Fragments arranged by size:
862 634 437 87 23
EcoICRI GAG'CTC
Cuts at: 0 1700 2043
Size: 1700 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1174 1201 1873 1936 2043
Size: 615 559 27 672 63 107
Fragments arranged by size:
672 615 559 107 63 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 994 1411 2043
Size: 994 417 632
Fragments arranged by size:
994 632 417
EcoO109I rG'GnC_Cy
Cuts at: 0 121 975 976 1072 1974 2043
Size: 121 854 1 96 902 69
Fragments arranged by size:
902 854 121 96 69 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 671 2043
Size: 671 1372
HaeII r_GCGC'y
Cuts at: 0 1577 1762 2043
Size: 1577 185 281
Fragments arranged by size:
1577 281 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1028 1060 1660 1672 1692 1704 1846 1878
Size: 1028 32 600 12 20 12 142 32
Cuts at: 1878 2043
Size: 165
Fragments arranged by size:
1028 600 165 142 32 32 20 12
12
HindIII A'AGCT_T
Cuts at: 0 601 2043
Size: 601 1442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1658 2043
Size: 956 702 385
Fragments arranged by size:
956 702 385
MscI TGG'CCA
Cuts at: 0 683 1411 2043
Size: 683 728 632
Fragments arranged by size:
728 683 632
MspA1I CmG'CkG
Cuts at: 0 849 1057 1379 1652 2004 2034 2043
Size: 849 208 322 273 352 30 9
Fragments arranged by size:
849 352 322 273 208 30 9
NaeI GCC'GGC
Cuts at: 0 1962 2043
Size: 1962 81
NcoI C'CATG_G
Cuts at: 0 1322 1331 2043
Size: 1322 9 712
Fragments arranged by size:
1322 712 9
NgoMIV G'CCGG_C
Cuts at: 0 1960 2043
Size: 1960 83
NspI r_CATG'y
Cuts at: 0 562 946 2028 2043
Size: 562 384 1082 15
Fragments arranged by size:
1082 562 384 15
PasI CC'CwG_GG
Cuts at: 0 1519 2043
Size: 1519 524
PflMI CCAn_nnn'nTGG
Cuts at: 0 1323 2043
Size: 1323 720
PfoI T'CCnGG_A
Cuts at: 0 890 1046 1082 1178 2043
Size: 890 156 36 96 865
Fragments arranged by size:
890 865 156 96 36
PmlI CAC'GTG
Cuts at: 0 801 1910 2043
Size: 801 1109 133
Fragments arranged by size:
1109 801 133
PpuMI rG'GwC_Cy
Cuts at: 0 121 1974 2043
Size: 121 1853 69
Fragments arranged by size:
1853 121 69
PspOMI G'GGCC_C
Cuts at: 0 975 1548 2043
Size: 975 573 495
Fragments arranged by size:
975 573 495
PspXI vC'TCGA_Gb
Cuts at: 0 241 2043
Size: 241 1802
PstI C_TGCA'G
Cuts at: 0 218 701 2043
Size: 218 483 1342
Fragments arranged by size:
1342 483 218
PvuII CAG'CTG
Cuts at: 0 2004 2034 2043
Size: 2004 30 9
Fragments arranged by size:
2004 30 9
SacI G_AGCT'C
Cuts at: 0 1702 2043
Size: 1702 341
SanDI GG'GwC_CC
Cuts at: 0 1974 2043
Size: 1974 69
SapI GCTCTTCn'nnn_
Cuts at: 0 1519 2043
Size: 1519 524
SfcI C'TryA_G
Cuts at: 0 214 697 1902 2043
Size: 214 483 1205 141
Fragments arranged by size:
1205 483 214 141
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 983 2043
Size: 983 1060
SmlI C'TyrA_G
Cuts at: 0 241 346 1762 2043
Size: 241 105 1416 281
Fragments arranged by size:
1416 281 241 105
SpeI A'CTAG_T
Cuts at: 0 1936 2043
Size: 1936 107
SphI G_CATG'C
Cuts at: 0 946 2043
Size: 946 1097
StuI AGG'CCT
Cuts at: 0 725 1850 2043
Size: 725 1125 193
Fragments arranged by size:
1125 725 193
StyI C'CwwG_G
Cuts at: 0 230 524 1322 1331 1412 2043
Size: 230 294 798 9 81 631
Fragments arranged by size:
798 631 294 230 81 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 769 2043
Size: 769 1274
TatI w'GTAC_w
Cuts at: 0 764 2043
Size: 764 1279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 1499 1531 1966 1998 2043
Size: 1201 32 266 32 435 32 45
Fragments arranged by size:
1201 435 266 45 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1924 2043
Size: 1924 119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1227 1329 2043
Size: 1227 102 714
Fragments arranged by size:
1227 714 102
XhoI C'TCGA_G
Cuts at: 0 241 2043
Size: 241 1802
ZraI GAC'GTC
Cuts at: 0 489 2043
Size: 489 1554
Enzymes that do cut:
AarI AatII AcuI AflIII AloI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII MmeI MscI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SapI SfcI SfiI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BfrBI BglII BmtI BplI BsiEI
BsiWI BsmI BspHI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SrfI SspI SwaI XbaI
XmaI XmnI