Restriction map for
pFN21AA0472
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoT22I FseI FspI HgaI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SfuI SgrAI SmaI SnaBI
SphI SspBI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1324 GACGTC GACGT^C
AccIII 1766 TCCGGA T^CCGGA
AflIII 2257 ACACGT A^C[GA][CT]GT
BbeI 452 GGCGCC GGCGC^C
BbsI 2618 GTCTTC GAAGAC(2/6)
BcgI 1791 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 975 GGATAC GTATCC(6/5)
BclI 1439 TGATCA T^GATCA
BpuAI 2618 GTCTTC GAAGAC(2/6)
BsaWI 1766 TCCGGA [AT]^CCGG[AT]
BseAI 1766 TCCGGA T^CCGGA
BsiEI 93 CGGCCG CG[GA][CT]^CG
BsmBI 36 CGTCTC CGTCTC(1/5)
BspEI 1766 TCCGGA T^CCGGA
BspHI 2726 TCATGA T^CATGA
BsrBI 2203 GAGCGG CCGCTC(-3/-3)
BsrDI 2181 CATTGC GCAATG(2/0)
BsrFI 1838 GCCGGT [GA]^CCGG[CT]
BssSI 1789 CACGAG CACGAG(-5/-1)
Bst1107I 1996 GTATAC GTA^TAC
BstZ17I 1996 GTATAC GTA^TAC
Cfr10I 1838 GCCGGT [GA]^CCGG[CT]
DraI 1318 TTTAAA TTT^AAA
DraIII 1042 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1324 GACGTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 93 CGGCCG C^GGCCG
EclXI 93 CGGCCG C^GGCCG
Eco52I 93 CGGCCG C^GGCCG
EcoRI 2504 GAATTC G^AATTC
EcoRV 2329 GATATC GAT^ATC
FbaI 1439 TGATCA T^GATCA
KasI 452 GGCGCC G^GCGCC
MroI 1766 TCCGGA T^CCGGA
NarI 452 GGCGCC GG^CGCC
NdeI 2443 CATATG CA^TATG
NheI 2566 GCTAGC G^CTAGC
PshAI 480 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2726 TCATGA T^CATGA
SbfI 229 CCTGCAGG CCTGCA^GG
SfiI 1828 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 452 GGCGCC GGC^GCC
SmlI 1026 CTTGAG C^T[CT][GA]AG
SpeI 516 ACTAGT A^CTAGT
Sse8387I 229 CCTGCAGG CCTGCA^GG
SspI 1219 AATATT AAT^ATT
TaqI 1182 TCGA T^CGA
TthHB8I 1182 TCGA T^CGA
XcmI 413 CCACCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 1996 GTATAC GT^[AC][GT]AC
2491 GTCTAC
AcsI 2089 GAATTT [GA]^AATT[CT]
2504 GAATTC
AcyI 452 GGCGCC G[GA]^CG[CT]C
1324 GACGTC
AfaI 306 GTAC GT^AC
2472 GTAC
Alw44I 520 GTGCAC G^TGCAC
1093 GTGCAC
ApaLI 520 GTGCAC G^TGCAC
1093 GTGCAC
ApoI 2089 GAATTT [GA]^AATT[CT]
2504 GAATTC
AvaI 39 CTCGGG C^[CT]CG[GA]G
482 CTCGGG
BalI 969 TGGCCA TGG^CCA
1827 TGGCCA
BbvCI 1725 CCTCAGC CCTCAGC(-5/-2)
1866 GCTGAGG
BglII 1556 AGATCT A^GATCT
1857 AGATCT
BsaHI 452 GGCGCC G[GA]^CG[CT]C
1324 GACGTC
BsgI 2302 CTGCAC GTGCAG(16/14)
400 GTGCAG
BsoBI 39 CTCGGG C^[CT]CG[GA]G
482 CTCGGG
Hin1I 452 GGCGCC G[GA]^CG[CT]C
1324 GACGTC
HindIII 445 AAGCTT A^AGCTT
1467 AAGCTT
MluNI 969 TGGCCA TGG^CCA
1827 TGGCCA
MscI 969 TGGCCA TGG^CCA
1827 TGGCCA
NcoI 19 CCATGG C^CATGG
2139 CCATGG
NspI 992 GCATGT [GA]CATG^[CT]
1136 ACATGC
PpuMI 42 GGGTCCC [GA]G^G[AT]CC[CT]
1145 GGGACCT
PvuII 376 CAGCTG CAG^CTG
1816 CAGCTG
RsaI 306 GTAC GT^AC
2472 GTAC
SexAI 1043 ACCAGGT A^CC[AT]GGT
1088 ACCTGGT
StuI 684 AGGCCT AGG^CCT
2686 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AocI Asp700I AspHI AvaII BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI