Restriction map for
pFN21AA0472
(Linear) (Six-base) MAPSORT of: KIAA0472.seq Check: 7554 from: 1 to: 2787
KIAA0472.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 393 1680 2787
Size: 393 1287 1107
Fragments arranged by size:
1287 1107 393
AatII G_ACGT'C
Cuts at: 0 1328 2787
Size: 1328 1459
AccI GT'mk_AC
Cuts at: 0 1997 2492 2787
Size: 1997 495 295
Fragments arranged by size:
1997 495 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 2254 2787
Size: 778 1476 533
Fragments arranged by size:
1476 778 533
AflIII A'CryG_T
Cuts at: 0 2257 2787
Size: 2257 530
AleI CACnn'nnGTG
Cuts at: 0 1660 2787
Size: 1660 1127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2203 2384 2787
Size: 2203 181 403
Fragments arranged by size:
2203 403 181
AlwNI CAG_nnn'CTG
Cuts at: 0 132 950 1503 1971 2533 2787
Size: 132 818 553 468 562 254
Fragments arranged by size:
818 562 553 468 254 132
ApaLI G'TGCA_C
Cuts at: 0 520 1093 2787
Size: 520 573 1694
Fragments arranged by size:
1694 573 520
ApoI r'AATT_y
Cuts at: 0 2089 2504 2787
Size: 2089 415 283
Fragments arranged by size:
2089 415 283
AvaI C'yCGr_G
Cuts at: 0 39 482 2787
Size: 39 443 2305
Fragments arranged by size:
2305 443 39
BanI G'GyrC_C
Cuts at: 0 15 452 1357 2787
Size: 15 437 905 1430
Fragments arranged by size:
1430 905 437 15
BanII G_rGCy'C
Cuts at: 0 35 856 966 1669 2607 2787
Size: 35 821 110 703 938 180
Fragments arranged by size:
938 821 703 180 110 35
BbeI G_GCGC'C
Cuts at: 0 456 2787
Size: 456 2331
BbsI GAAGACnn'nnnn_
Cuts at: 0 2611 2787
Size: 2611 176
BbvCI CC'TCA_GC
Cuts at: 0 1726 1867 2787
Size: 1726 141 920
Fragments arranged by size:
1726 920 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1780 1814 2787
Size: 1780 34 973
Fragments arranged by size:
1780 973 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 969 2787
Size: 969 1818
BclI T'GATC_A
Cuts at: 0 1439 2787
Size: 1439 1348
BglI GCCn_nnn'nGGC
Cuts at: 0 56 233 1835 2787
Size: 56 177 1602 952
Fragments arranged by size:
1602 952 177 56
BglII A'GATC_T
Cuts at: 0 1556 1857 2787
Size: 1556 301 930
Fragments arranged by size:
1556 930 301
Bme1580I G_kGCm'C
Cuts at: 0 524 1097 2037 2787
Size: 524 573 940 750
Fragments arranged by size:
940 750 573 524
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 859 1969 1981 2022 2787
Size: 558 301 1110 12 41 765
Fragments arranged by size:
1110 765 558 301 41 12
BmtI G_CTAG'C
Cuts at: 0 2570 2787
Size: 2570 217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 54 2621 2787
Size: 54 2567 166
Fragments arranged by size:
2567 166 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 595 643 1525 2039 2160 2272 2701
Size: 295 300 48 882 514 121 112 429
Cuts at: 2701 2787
Size: 86
Fragments arranged by size:
882 514 429 300 295 121 112 86
48
Bpu10I CC'TnA_GC
Cuts at: 0 1726 1867 2526 2787
Size: 1726 141 659 261
Fragments arranged by size:
1726 659 261 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 2787
Size: 1047 1740
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 547 2111 2787
Size: 133 414 1564 676
Fragments arranged by size:
1564 676 414 133
BsaHI Gr'CG_yC
Cuts at: 0 453 1325 2787
Size: 453 872 1462
Fragments arranged by size:
1462 872 453
BsaWI w'CCGG_w
Cuts at: 0 1766 2787
Size: 1766 1021
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1183 1213 1337 1367 2787
Size: 1183 30 124 30 1420
Fragments arranged by size:
1420 1183 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 183 448 580 861 2182 2650 2787
Size: 183 265 132 281 1321 468 137
Fragments arranged by size:
1321 468 281 265 183 137 132
BseYI C'CCAG_C
Cuts at: 0 423 2239 2787
Size: 423 1816 548
Fragments arranged by size:
1816 548 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 2287 2787
Size: 421 1866 500
Fragments arranged by size:
1866 500 421
BsiEI CG_ry'CG
Cuts at: 0 96 2787
Size: 96 2691
BsiHKAI G_wGCw'C
Cuts at: 0 172 524 1033 1097 2192 2787
Size: 172 352 509 64 1095 595
Fragments arranged by size:
1095 595 509 352 172 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 42 2787
Size: 42 2745
Bsp1286I G_dGCh'C
Cuts at: 0 35 172 524 856 966 1033 1097 1669
Size: 35 137 352 332 110 67 64 572
Cuts at: 1669 2037 2192 2607 2787
Size: 368 155 415 180
Fragments arranged by size:
572 415 368 352 332 180 155 137
110 67 64 35
BspEI T'CCGG_A
Cuts at: 0 1766 2787
Size: 1766 1021
BspHI T'CATG_A
Cuts at: 0 2726 2787
Size: 2726 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 393 1157 1680 2787
Size: 140 253 764 523 1107
Fragments arranged by size:
1107 764 523 253 140
BsrBI CCG'CTC
Cuts at: 0 2205 2787
Size: 2205 582
BsrDI GCAATG_nn'
Cuts at: 0 2180 2787
Size: 2180 607
BsrFI r'CCGG_y
Cuts at: 0 1838 2787
Size: 1838 949
BssSI C'ACGA_G
Cuts at: 0 1789 2787
Size: 1789 998
BstXI CCAn_nnnn'nTGG
Cuts at: 0 423 885 1170 1683 2787
Size: 423 462 285 513 1104
Fragments arranged by size:
1104 513 462 423 285
BstYI r'GATC_y
Cuts at: 0 570 1388 1556 1857 2133 2217 2787
Size: 570 818 168 301 276 84 570
Fragments arranged by size:
818 570 570 301 276 168 84
BstZ17I GTA'TAC
Cuts at: 0 1998 2787
Size: 1998 789
Bsu36I CC'TnA_GG
Cuts at: 0 293 563 1459 2546 2787
Size: 293 270 896 1087 241
Fragments arranged by size:
1087 896 293 270 241
BtgI C'CryG_G
Cuts at: 0 19 2139 2483 2787
Size: 19 2120 344 304
Fragments arranged by size:
2120 344 304 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 251 2787
Size: 251 2536
BtsI GCAGTG_nn'
Cuts at: 0 435 1095 1101 1430 2787
Size: 435 660 6 329 1357
Fragments arranged by size:
1357 660 435 329 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2148 2183 2787
Size: 2148 35 604
Fragments arranged by size:
2148 604 35
DraI TTT'AAA
Cuts at: 0 1320 2787
Size: 1320 1467
DraIII CAC_nnn'GTG
Cuts at: 0 1047 2787
Size: 1047 1740
DrdI GACnn_nn'nnGTC
Cuts at: 0 1330 2787
Size: 1330 1457
EaeI y'GGCC_r
Cuts at: 0 93 351 969 1827 2787
Size: 93 258 618 858 960
Fragments arranged by size:
960 858 618 258 93
EagI C'GGCC_G
Cuts at: 0 93 2787
Size: 93 2694
EarI CTCTTCn'nnn_
Cuts at: 0 293 1687 2677 2787
Size: 293 1394 990 110
Fragments arranged by size:
1394 990 293 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 77 2787
Size: 77 2710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 595 643 778 1525 2039 2160 2254
Size: 295 300 48 135 747 514 121 94
Cuts at: 2254 2272 2701 2787
Size: 18 429 86
Fragments arranged by size:
747 514 429 300 295 135 121 94
86 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 876 2682 2787
Size: 352 524 1806 105
Fragments arranged by size:
1806 524 352 105
EcoO109I rG'GnC_Cy
Cuts at: 0 43 1080 1146 2787
Size: 43 1037 66 1641
Fragments arranged by size:
1641 1037 66 43
EcoRI G'AATT_C
Cuts at: 0 2504 2787
Size: 2504 283
EcoRV GAT'ATC
Cuts at: 0 2331 2787
Size: 2331 456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1940 2787
Size: 1940 847
HaeII r_GCGC'y
Cuts at: 0 456 499 1733 2787
Size: 456 43 1234 1054
Fragments arranged by size:
1234 1054 456 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 754 786 930 962 2787
Size: 465 32 257 32 144 32 1825
Fragments arranged by size:
1825 465 257 144 32 32 32
HindIII A'AGCT_T
Cuts at: 0 445 1467 2787
Size: 445 1022 1320
Fragments arranged by size:
1320 1022 445
KasI G'GCGC_C
Cuts at: 0 452 2787
Size: 452 2335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1407 1455 2787
Size: 1407 48 1332
Fragments arranged by size:
1407 1332 48
MscI TGG'CCA
Cuts at: 0 971 1829 2787
Size: 971 858 958
Fragments arranged by size:
971 958 858
MslI CAynn'nnrTG
Cuts at: 0 761 997 1660 2072 2185 2787
Size: 761 236 663 412 113 602
Fragments arranged by size:
761 663 602 412 236 113
MspA1I CmG'CkG
Cuts at: 0 378 1143 1818 2787
Size: 378 765 675 969
Fragments arranged by size:
969 765 675 378
NarI GG'CG_CC
Cuts at: 0 453 2787
Size: 453 2334
NcoI C'CATG_G
Cuts at: 0 19 2139 2787
Size: 19 2120 648
Fragments arranged by size:
2120 648 19
NdeI CA'TA_TG
Cuts at: 0 2444 2787
Size: 2444 343
NheI G'CTAG_C
Cuts at: 0 2566 2787
Size: 2566 221
NspI r_CATG'y
Cuts at: 0 996 1140 2787
Size: 996 144 1647
Fragments arranged by size:
1647 996 144
PfoI T'CCnGG_A
Cuts at: 0 1391 2787
Size: 1391 1396
PpuMI rG'GwC_Cy
Cuts at: 0 43 1146 2787
Size: 43 1103 1641
Fragments arranged by size:
1641 1103 43
PshAI GACnn'nnGTC
Cuts at: 0 484 2787
Size: 484 2303
PstI C_TGCA'G
Cuts at: 0 234 260 1154 1499 2787
Size: 234 26 894 345 1288
Fragments arranged by size:
1288 894 345 234 26
PvuII CAG'CTG
Cuts at: 0 378 1818 2787
Size: 378 1440 969
Fragments arranged by size:
1440 969 378
SanDI GG'GwC_CC
Cuts at: 0 43 2787
Size: 43 2744
SbfI CC_TGCA'GG
Cuts at: 0 234 2787
Size: 234 2553
SexAI A'CCwGG_T
Cuts at: 0 1043 1088 2787
Size: 1043 45 1699
Fragments arranged by size:
1699 1043 45
SfcI C'TryA_G
Cuts at: 0 230 256 1150 1495 2787
Size: 230 26 894 345 1292
Fragments arranged by size:
1292 894 345 230 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1835 2787
Size: 1835 952
SfoI GGC'GCC
Cuts at: 0 454 2787
Size: 454 2333
SmlI C'TyrA_G
Cuts at: 0 1026 2787
Size: 1026 1761
SpeI A'CTAG_T
Cuts at: 0 516 2787
Size: 516 2271
SspI AAT'ATT
Cuts at: 0 1221 2787
Size: 1221 1566
StuI AGG'CCT
Cuts at: 0 686 2688 2787
Size: 686 2002 99
Fragments arranged by size:
2002 686 99
StyI C'CwwG_G
Cuts at: 0 19 681 1076 2139 2787
Size: 19 662 395 1063 648
Fragments arranged by size:
1063 662 648 395 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 1650 2787
Size: 700 950 1137
Fragments arranged by size:
1137 950 700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 420 2787
Size: 420 2367
XmnI GAAnn'nnTTC
Cuts at: 0 151 727 2618 2787
Size: 151 576 1891 169
Fragments arranged by size:
1891 576 169 151
ZraI GAC'GTC
Cuts at: 0 1326 2787
Size: 1326 1461
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HindIII KasI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NspI
PfoI PpuMI PshAI PstI PvuII SanDI SbfI SexAI
SfcI SfiI SfoI SmlI SpeI SspI StuI StyI
TaqII XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BfrBI BlpI
BmgBI BsaAI BsaBI BsiWI BsmI BsrGI BssHII BstAPI
BstBI BstEII ClaI EcoICRI FseI FspI FspAI HincII
HpaI KpnI MfeI MluI NaeI NgoMIV NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI TatI TstI Tth111I XbaI XhoI XmaI