Restriction map for
pFN21AA0459
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MflI MluI MroI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 86 CGCG CG^CG
AlwI 563 GGATC GGATC(4/5)
ApaI 92 GGGCCC GGGCC^C
AscI 84 GGCGCGCC GG^CGCGCC
Asp700I 1170 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 950 GGACC G^G[AT]CC
BclI 246 TGATCA T^GATCA
BfaI 980 CTAG C^TAG
BsgI 587 CTGCAC GTGCAG(16/14)
BsmAI 292 GAGAC GTCTC(1/5)
BsrDI 404 GCAATG GCAATG(2/0)
BsrFI 26 GCCGGC [GA]^CCGG[CT]
BssHII 85 GCGCGC G^CGCGC
BstAPI 793 GCAATAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1158 GGTCACC G^GT[ACGT]ACC
BstPI 1158 GGTCACC G^GT[ACGT]ACC
BstUI 86 CGCG CG^CG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
EcoNI 96 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1158 GGTCACC G^GT[ACGT]ACC
FbaI 246 TGATCA T^GATCA
MaeI 980 CTAG C^TAG
MvnI 86 CGCG CG^CG
NaeI 26 GCCGGC GCC^GGC
NgoMIV 26 GCCGGC G^CCGGC
NspI 366 GCATGT [GA]CATG^[CT]
PflMI 756 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 178 CTGCAG CTGCA^G
PvuII 175 CAGCTG CAG^CTG
SacI 236 GAGCTC GAGCT^C
SapI 21 GAAGAGC GCTCTTC(1/4)
SfcI 178 CTGCAG C^T[GA][CT]AG
SspI 781 AATATT AAT^ATT
TaqI 864 TCGA T^CGA
TthHB8I 864 TCGA T^CGA
Van91I 756 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1170 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 392 GTAC GT^AC
491 GTAC
AlwNI 181 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
757 CAGAACCTG
AspHI 236 GAGCTC G[AT]GC[AT]^C
541 GAGCAC
BalI 98 TGGCCA TGG^CCA
371 TGGCCA
BbsI 673 GAAGAC GAAGAC(2/6)
1170 GAAGAC
BglI 26 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
373 GCCAGTTTGGC
BpmI 595 CTCCAG CTGGAG(16/14)
1099 CTGGAG
BpuAI 673 GAAGAC GAAGAC(2/6)
1170 GAAGAC
BsiEI 28 CGGCCG CG[GA][CT]^CG
118 CGGCCG
BsiHKAI 236 GAGCTC G[AT]GC[AT]^C
541 GAGCAC
BspMI 585 ACCTGC ACCTGC(4/8)
1155 GCAGGT
DdeI 576 CTCAG C^T[ACGT]AG
682 CTGAG
DraII 92 GGGCCCT [GA]G^G[ACGT]CC[CT]
182 AGGCCCT
EagI 28 CGGCCG C^GGCCG
118 CGGCCG
EclXI 28 CGGCCG C^GGCCG
118 CGGCCG
Eco52I 28 CGGCCG C^GGCCG
118 CGGCCG
EcoO109I 92 GGGCCCT [GA]G^G[ACGT]CC[CT]
182 AGGCCCT
MluNI 98 TGGCCA TGG^CCA
371 TGGCCA
MscI 98 TGGCCA TGG^CCA
371 TGGCCA
MslI 619 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CACCTTGGTG
NlaIII 306 CATG CATG^
367 CATG
PleI 667 GACTC GAGTC(4/5)
124 GAGTC
RsaI 392 GTAC GT^AC
491 GTAC
SmlI 277 CTCAAG C^T[CT][GA]AG
850 CTTGAG
Tsp45I 1021 GTCAC ^GT[GC]AC
1159 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MfeI MnlI MseI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI